Plant Circular RNA Database

Detail information of AT1G02300_circ_g.6

			
						 General Information
					
						CircRNA Name
						AT1G02300_circ_g.6
					
						ID in PlantcircBase
						ath_circ_000256
					
						Alias
						At_ciR399, AT1G02300_C1
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr1: 454415-455040  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, PcircRNA_finder, circRNA_finder, find_circ, CIRI-full, CIRI2
					
						Parent gene
						
							AT1G02300						
					
						Parent gene annotation
						
							Cathepsin B-like protease 1
							
						Parent gene strand
						+
					
						Alternative splicing
						
							1_circ_ag.36 AT1G02090_circ_g.1 AT1G02090_circ_g.2 AT1G02090_circ_g.3 AT1G02090_circ_g.4 AT1G02100_circ_g.1 AT1G02100_circ_g.2 AT1G02110_circ_g.1 AT1G02120_circ_g.1 AT1G02120_circ_g.2 AT1G02120_circ_g.3 AT1G02120_circ_g.4 AT1G02120_circ_g.5 AT1G02120_circ_g.6 AT1G02120_circ_g.7 AT1G02120_circ_g.8 AT1G02120_circ_g.9 AT1G02130_circ_g.1 AT1G02130_circ_g.2 AT1G02130_circ_g.3 AT1G02140_circ_g.1 AT1G02145_circ_g.1 AT1G02145_circ_g.2 AT1G02145_circ_g.3 AT1G02145_circ_g.4 AT1G02145_circ_g.5 AT1G02145_circ_g.6 AT1G02145_circ_g.7 AT1G02145_circ_g.8 AT1G02145_circ_g.9 AT1G02170_circ_g.1 AT1G02170_circ_g.2 AT1G02190_circ_g.1 1_circ_ag.2 AT1G02190_circ_g.3 AT1G02190_circ_g.4 AT1G02190_circ_g.5 AT1G02205_circ_g.1 AT1G02205_circ_g.2 AT1G02205_circ_g.3 AT1G02205_circ_g.4 AT1G02205_circ_g.5 1_circ_igg.1 AT1G02280_circ_g.1 AT1G02300_circ_g.1 1_circ_ag.2 AT1G02300_circ_g.3 1_circ_ag.4 1_circ_ag.5 AT1G02300_circ_g.7 1_circ_ag.8 AT1G02300_circ_g.9 1_circ_ag.10 1_circ_ag.11 1_circ_ag.12 AT1G02305_circ_g.1 AT1G02305_circ_g.2 AT1G02305_circ_g.3 AT1G02305_circ_g.4 AT1G02305_circ_g.5 AT1G02305_circ_g.6 AT1G02305_circ_g.7 						
					
						Support reads
						7/17
					
						Tissues
						leaf/whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT1G02300.1:3

General Information
CircRNA Name	AT1G02300_circ_g.6
ID in PlantcircBase	ath_circ_000256
Alias	At_ciR399, AT1G02300_C1
Organism	Arabidpsis thaliana
Position	chr1: 454415-455040 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, PcircRNA_finder, circRNA_finder, find_circ, CIRI-full, CIRI2
Parent gene	AT1G02300
Parent gene annotation	Cathepsin B-like protease 1
Parent gene strand	+
Alternative splicing	1_circ_ag.36 AT1G02090_circ_g.1 AT1G02090_circ_g.2 AT1G02090_circ_g.3 AT1G02090_circ_g.4 AT1G02100_circ_g.1 AT1G02100_circ_g.2 AT1G02110_circ_g.1 AT1G02120_circ_g.1 AT1G02120_circ_g.2 AT1G02120_circ_g.3 AT1G02120_circ_g.4 AT1G02120_circ_g.5 AT1G02120_circ_g.6 AT1G02120_circ_g.7 AT1G02120_circ_g.8 AT1G02120_circ_g.9 AT1G02130_circ_g.1 AT1G02130_circ_g.2 AT1G02130_circ_g.3 AT1G02140_circ_g.1 AT1G02145_circ_g.1 AT1G02145_circ_g.2 AT1G02145_circ_g.3 AT1G02145_circ_g.4 AT1G02145_circ_g.5 AT1G02145_circ_g.6 AT1G02145_circ_g.7 AT1G02145_circ_g.8 AT1G02145_circ_g.9 AT1G02170_circ_g.1 AT1G02170_circ_g.2 AT1G02190_circ_g.1 1_circ_ag.2 AT1G02190_circ_g.3 AT1G02190_circ_g.4 AT1G02190_circ_g.5 AT1G02205_circ_g.1 AT1G02205_circ_g.2 AT1G02205_circ_g.3 AT1G02205_circ_g.4 AT1G02205_circ_g.5 1_circ_igg.1 AT1G02280_circ_g.1 AT1G02300_circ_g.1 1_circ_ag.2 AT1G02300_circ_g.3 1_circ_ag.4 1_circ_ag.5 AT1G02300_circ_g.7 1_circ_ag.8 AT1G02300_circ_g.9 1_circ_ag.10 1_circ_ag.11 1_circ_ag.12 AT1G02305_circ_g.1 AT1G02305_circ_g.2 AT1G02305_circ_g.3 AT1G02305_circ_g.4 AT1G02305_circ_g.5 AT1G02305_circ_g.6 AT1G02305_circ_g.7
Support reads	7/17
Tissues	leaf/whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT1G02300.1:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CAAATCAGGAGTATACAAGTACATAACGGGTACTAAGATTGGAGGTCATGCAGTTAAGCTCATT
GGCTGGGGAACTTCTGATGACGGCGAAGATTATTGGaatgtttccttatctgccaatgatgtca
tagcatgttgtggattactttgcggttttggttgtaatggtggtttcccaatgggtgcatggtt
gtacttta

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 459
Transcript exons: 454415-454540,454622-454840,454927-455040
AATGTTTCCTTATCTGCCAATGATGTCATAGCATGTTGTGGATTACTTTGCGGTTTTGGTTGTA
ATGGTGGTTTCCCAATGGGTGCATGGTTGTACTTTAAGTACCACGGTGTAGTAACCCAAGAGTG
TGATCCATACTTTGACAATACTGGTTGCTCTCACCCGGGATGTGAACCCACTTACCCTACACCA
AAATGTGAGAGGAAATGTGTAAGCAGAAACCAGCTATGGGGTGAATCAAAACACTATGGTGTCG
GCGCATACAGAATCAATCCTGACCCTCAAGACATTATGGCTGAGGTTTACAAGAATGGACCTGT
CGAGGTTGCTTTTACCGTTTACGAGGACTTTGCACATTACAAATCAGGAGTATACAAGTACATA
ACGGGTACTAAGATTGGAGGTCATGCAGTTAAGCTCATTGGCTGGGGAACTTCTGATGACGGCG
AAGATTATTGG
						
						Genomic sequence
						
							AATGTTTCCTTATCTGCCAATGATGTCATAGCATGTTGTGGATTACTTTGCGGTTTTGGTTGTA
ATGGTGGTTTCCCAATGGGTGCATGGTTGTACTTTAAGTACCACGGTGTAGTAACCCAAGAGGT
AAATTCTCAGCAAAGAACAAATAATAATGGCAATGTGGTCAAAGTGTATTGAACTTTTGTAATG
CTGTTGGTTCTTCAGTGTGATCCATACTTTGACAATACTGGTTGCTCTCACCCGGGATGTGAAC
CCACTTACCCTACACCAAAATGTGAGAGGAAATGTGTAAGCAGAAACCAGCTATGGGGTGAATC
AAAACACTATGGTGTCGGCGCATACAGAATCAATCCTGACCCTCAAGACATTATGGCTGAGGTT
TACAAGAATGGACCTGTCGAGGTTGCTTTTACCGTTTACGAGGTAATGAAAAAGATTTTTGATT
GATCAAACCATATTGATTATATCTTGGTGAAGTCCACATTTTCATGTATCTTTTTGTTCTTCAG
GACTTTGCACATTACAAATCAGGAGTATACAAGTACATAACGGGTACTAAGATTGGAGGTCATG
CAGTTAAGCTCATTGGCTGGGGAACTTCTGATGACGGCGAAGATTATTGG

Sequences
Splice junction sequence	CAAATCAGGAGTATACAAGTACATAACGGGTACTAAGATTGGAGGTCATGCAGTTAAGCTCATT GGCTGGGGAACTTCTGATGACGGCGAAGATTATTGGaatgtttccttatctgccaatgatgtca tagcatgttgtggattactttgcggttttggttgtaatggtggtttcccaatgggtgcatggtt gtacttta
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 459 Transcript exons: 454415-454540,454622-454840,454927-455040 AATGTTTCCTTATCTGCCAATGATGTCATAGCATGTTGTGGATTACTTTGCGGTTTTGGTTGTA ATGGTGGTTTCCCAATGGGTGCATGGTTGTACTTTAAGTACCACGGTGTAGTAACCCAAGAGTG TGATCCATACTTTGACAATACTGGTTGCTCTCACCCGGGATGTGAACCCACTTACCCTACACCA AAATGTGAGAGGAAATGTGTAAGCAGAAACCAGCTATGGGGTGAATCAAAACACTATGGTGTCG GCGCATACAGAATCAATCCTGACCCTCAAGACATTATGGCTGAGGTTTACAAGAATGGACCTGT CGAGGTTGCTTTTACCGTTTACGAGGACTTTGCACATTACAAATCAGGAGTATACAAGTACATA ACGGGTACTAAGATTGGAGGTCATGCAGTTAAGCTCATTGGCTGGGGAACTTCTGATGACGGCG AAGATTATTGG
Genomic sequence	AATGTTTCCTTATCTGCCAATGATGTCATAGCATGTTGTGGATTACTTTGCGGTTTTGGTTGTA ATGGTGGTTTCCCAATGGGTGCATGGTTGTACTTTAAGTACCACGGTGTAGTAACCCAAGAGGT AAATTCTCAGCAAAGAACAAATAATAATGGCAATGTGGTCAAAGTGTATTGAACTTTTGTAATG CTGTTGGTTCTTCAGTGTGATCCATACTTTGACAATACTGGTTGCTCTCACCCGGGATGTGAAC CCACTTACCCTACACCAAAATGTGAGAGGAAATGTGTAAGCAGAAACCAGCTATGGGGTGAATC AAAACACTATGGTGTCGGCGCATACAGAATCAATCCTGACCCTCAAGACATTATGGCTGAGGTT TACAAGAATGGACCTGTCGAGGTTGCTTTTACCGTTTACGAGGTAATGAAAAAGATTTTTGATT GATCAAACCATATTGATTATATCTTGGTGAAGTCCACATTTTCATGTATCTTTTTGTTCTTCAG GACTTTGCACATTACAAATCAGGAGTATACAAGTACATAACGGGTACTAAGATTGGAGGTCATG CAGTTAAGCTCATTGGCTGGGGAACTTCTGATGACGGCGAAGATTATTGG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.3762462

Conservation Information
Conserved circRNAs	NA
PMCS	0.3762462

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							454493-455037(+)
						
						Potential amino acid sequence
						
							MGAWLYFKYHGVVTQECDPYFDNTGCSHPGCEPTYPTPKCERKCVSRNQLWGESKHYGVGAYRI
NPDPQDIMAEVYKNGPVEVAFTVYEDFAHYKSGVYKYITGTKIGGHAVKLIGWGTSDDGEDYWN
VSLSANDVIACCGLLCGFGCNGGFPMGAWLYFKYHGVVTQECDPYFDNTGCSHPGCEPTYPTPK
CERKCVSRNQLWGESKHYGVGAYRINPDPQDIMAEVYKNGPVEVAFTVYEDFAHYKSGVYKYIT
GTKIGGHAVKLIGWGTSDDGEDYWNVSLSANDVIACCGLLCGFGCNGGFPMGAWLYFKYHGVVT
QECDPYFDNTGCSHPGCEPTYPTPKCERKCVSRNQLWGESKHYGVGAYRINPDPQDIMAEVYKN
GPVEVAFTVYEDFAHYKSGVYKYITGTKIGGHAVKLIGWGTSDDGEDYWNVSLSANDVIACCGL
LCGFGCNGGFPMGAWLYFKYHGVVTQECDPYFDNTGCSHPGCEPTYPTPKCERKCVSRNQLWGE
SKHYGVGAYRINPDPQDIMAEVYKNGPVEVAFTVYEDFAHYKSGVYKYITGTKIGGHAVKLIGW
GTSDDGEDYW(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						response to drought stress

Functional Information
Coding potential	Y
Potential coding position	454493-455037(+)
Potential amino acid sequence	MGAWLYFKYHGVVTQECDPYFDNTGCSHPGCEPTYPTPKCERKCVSRNQLWGESKHYGVGAYRI NPDPQDIMAEVYKNGPVEVAFTVYEDFAHYKSGVYKYITGTKIGGHAVKLIGWGTSDDGEDYWN VSLSANDVIACCGLLCGFGCNGGFPMGAWLYFKYHGVVTQECDPYFDNTGCSHPGCEPTYPTPK CERKCVSRNQLWGESKHYGVGAYRINPDPQDIMAEVYKNGPVEVAFTVYEDFAHYKSGVYKYIT GTKIGGHAVKLIGWGTSDDGEDYWNVSLSANDVIACCGLLCGFGCNGGFPMGAWLYFKYHGVVT QECDPYFDNTGCSHPGCEPTYPTPKCERKCVSRNQLWGESKHYGVGAYRINPDPQDIMAEVYKN GPVEVAFTVYEDFAHYKSGVYKYITGTKIGGHAVKLIGWGTSDDGEDYWNVSLSANDVIACCGL LCGFGCNGGFPMGAWLYFKYHGVVTQECDPYFDNTGCSHPGCEPTYPTPKCERKCVSRNQLWGE SKHYGVGAYRINPDPQDIMAEVYKNGPVEVAFTVYEDFAHYKSGVYKYITGTKIGGHAVKLIGW GTSDDGEDYW(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	response to drought stress

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017;Zhang et al., 2019

circRNA

Detail information of AT1G02300_circ_g.6

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