Plant Circular RNA Database

Detail information of AT5G25220_circ_g.5

			
						 General Information
					
						CircRNA Name
						AT5G25220_circ_g.5
					
						ID in PlantcircBase
						ath_circ_040349
					
						Alias
						At_ciR4012
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 8737164-8737557  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder, find_circ, CIRI-full
					
						Parent gene
						
							AT5G25220						
					
						Parent gene annotation
						
							KNAT3
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT5G25220_circ_g.4 AT5G25220_circ_g.6 AT5G25220_circ_g.7 AT5G25220_circ_g.8 						
					
						Support reads
						5/5
					
						Tissues
						leaf/whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G25220.2:2
AT5G25220.1:2

General Information
CircRNA Name	AT5G25220_circ_g.5
ID in PlantcircBase	ath_circ_040349
Alias	At_ciR4012
Organism	Arabidpsis thaliana
Position	chr5: 8737164-8737557 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, PcircRNA_finder, find_circ, CIRI-full
Parent gene	AT5G25220
Parent gene annotation	KNAT3
Parent gene strand	+
Alternative splicing	AT5G25220_circ_g.4 AT5G25220_circ_g.6 AT5G25220_circ_g.7 AT5G25220_circ_g.8
Support reads	5/5
Tissues	leaf/whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G25220.2:2 AT5G25220.1:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GGGATTAGATGTGTTGGGTTTTGGTCCTTTGATTCCTACTGAGAGTGAGAGGTCGTTGATGGAA
AGAGTTAGACAAGAACTTAAACATGAACTCAAACAGacacattatgtgttgctactgtgttcat
ttaaagagcaattgcaacaacatgtgcgtgttcatgcaatggaagctgtgatggcttgttggga
gattgagc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 306
Transcript exons: 8737164-8737284,8737373-8737557
ACACATTATGTGTTGCTACTGTGTTCATTTAAAGAGCAATTGCAACAACATGTGCGTGTTCATG
CAATGGAAGCTGTGATGGCTTGTTGGGAGATTGAGCAGTCTCTTCAAAGCTTAACAGGAGTGTC
TCCTGGAGAAGGGATGGGAGCAACAATGTCTGACGATGAAGATGAACAAGTAGAGAGTGATGCT
AATATGTTCGATGGGGGATTAGATGTGTTGGGTTTTGGTCCTTTGATTCCTACTGAGAGTGAGA
GGTCGTTGATGGAAAGAGTTAGACAAGAACTTAAACATGAACTCAAACAG
						
						Genomic sequence
						
							ACACATTATGTGTTGCTACTGTGTTCATTTAAAGAGCAATTGCAACAACATGTGCGTGTTCATG
CAATGGAAGCTGTGATGGCTTGTTGGGAGATTGAGCAGTCTCTTCAAAGCTTAACAGGTGAGAA
AATGAATCCTGTGATTAGCTCATGAATCCAATAGTTAGTTTTATGACATTGACAAGTTTTGTGT
TTTATTGGTGAGATTAGGAGTGTCTCCTGGAGAAGGGATGGGAGCAACAATGTCTGACGATGAA
GATGAACAAGTAGAGAGTGATGCTAATATGTTCGATGGGGGATTAGATGTGTTGGGTTTTGGTC
CTTTGATTCCTACTGAGAGTGAGAGGTCGTTGATGGAAAGAGTTAGACAAGAACTTAAACATGA
ACTCAAACAG

Sequences
Splice junction sequence	GGGATTAGATGTGTTGGGTTTTGGTCCTTTGATTCCTACTGAGAGTGAGAGGTCGTTGATGGAA AGAGTTAGACAAGAACTTAAACATGAACTCAAACAGacacattatgtgttgctactgtgttcat ttaaagagcaattgcaacaacatgtgcgtgttcatgcaatggaagctgtgatggcttgttggga gattgagc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 306 Transcript exons: 8737164-8737284,8737373-8737557 ACACATTATGTGTTGCTACTGTGTTCATTTAAAGAGCAATTGCAACAACATGTGCGTGTTCATG CAATGGAAGCTGTGATGGCTTGTTGGGAGATTGAGCAGTCTCTTCAAAGCTTAACAGGAGTGTC TCCTGGAGAAGGGATGGGAGCAACAATGTCTGACGATGAAGATGAACAAGTAGAGAGTGATGCT AATATGTTCGATGGGGGATTAGATGTGTTGGGTTTTGGTCCTTTGATTCCTACTGAGAGTGAGA GGTCGTTGATGGAAAGAGTTAGACAAGAACTTAAACATGAACTCAAACAG
Genomic sequence	ACACATTATGTGTTGCTACTGTGTTCATTTAAAGAGCAATTGCAACAACATGTGCGTGTTCATG CAATGGAAGCTGTGATGGCTTGTTGGGAGATTGAGCAGTCTCTTCAAAGCTTAACAGGTGAGAA AATGAATCCTGTGATTAGCTCATGAATCCAATAGTTAGTTTTATGACATTGACAAGTTTTGTGT TTTATTGGTGAGATTAGGAGTGTCTCCTGGAGAAGGGATGGGAGCAACAATGTCTGACGATGAA GATGAACAAGTAGAGAGTGATGCTAATATGTTCGATGGGGGATTAGATGTGTTGGGTTTTGGTC CTTTGATTCCTACTGAGAGTGAGAGGTCGTTGATGGAAAGAGTTAGACAAGAACTTAAACATGA ACTCAAACAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.36764592

Conservation Information
Conserved circRNAs	NA
PMCS	0.36764592

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							8737230-8737554(+)
						
						Potential amino acid sequence
						
							MEAVMACWEIEQSLQSLTGVSPGEGMGATMSDDEDEQVESDANMFDGGLDVLGFGPLIPTESER
SLMERVRQELKHELKQTHYVLLLCSFKEQLQQHVRVHAMEAVMACWEIEQSLQSLTGVSPGEGM
GATMSDDEDEQVESDANMFDGGLDVLGFGPLIPTESERSLMERVRQELKHELKQTHYVLLLCSF
KEQLQQHVRVHAMEAVMACWEIEQSLQSLTGVSPGEGMGATMSDDEDEQVESDANMFDGGLDVL
GFGPLIPTESERSLMERVRQELKHELKQTHYVLLLCSFKEQLQQHVRVHAMEAVMACWEIEQSL
QSLTGVSPGEGMGATMSDDEDEQVESDANMFDGGLDVLGFGPLIPTESERSLMERVRQELKHEL
KQ(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	8737230-8737554(+)
Potential amino acid sequence	MEAVMACWEIEQSLQSLTGVSPGEGMGATMSDDEDEQVESDANMFDGGLDVLGFGPLIPTESER SLMERVRQELKHELKQTHYVLLLCSFKEQLQQHVRVHAMEAVMACWEIEQSLQSLTGVSPGEGM GATMSDDEDEQVESDANMFDGGLDVLGFGPLIPTESERSLMERVRQELKHELKQTHYVLLLCSF KEQLQQHVRVHAMEAVMACWEIEQSLQSLTGVSPGEGMGATMSDDEDEQVESDANMFDGGLDVL GFGPLIPTESERSLMERVRQELKHELKQTHYVLLLCSFKEQLQQHVRVHAMEAVMACWEIEQSL QSLTGVSPGEGMGATMSDDEDEQVESDANMFDGGLDVLGFGPLIPTESERSLMERVRQELKHEL KQ(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of AT5G25220_circ_g.5

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