Plant Circular RNA Database

Detail information of AT5G25220_circ_g.4

			
						 General Information
					
						CircRNA Name
						AT5G25220_circ_g.4
					
						ID in PlantcircBase
						ath_circ_040348
					
						Alias
						Ath_circ_FC3518
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 8736569-8738208  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							AT5G25220						
					
						Parent gene annotation
						
							KNAT3
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT5G25220_circ_g.5 AT5G25220_circ_g.6 AT5G25220_circ_g.7 AT5G25220_circ_g.8 						
					
						Support reads
						NA
					
						Tissues
						aerial
					
						Exon boundary  
						
							No-No							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							TT-CG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G25220.1:6
AT5G25220.2:6

General Information
CircRNA Name	AT5G25220_circ_g.4
ID in PlantcircBase	ath_circ_040348
Alias	Ath_circ_FC3518
Organism	Arabidpsis thaliana
Position	chr5: 8736569-8738208 JBrowse»
Reference genome	TAIR10.38
Type	ue-circRNA
Identification method	find_circ
Parent gene	AT5G25220
Parent gene annotation	KNAT3
Parent gene strand	+
Alternative splicing	AT5G25220_circ_g.5 AT5G25220_circ_g.6 AT5G25220_circ_g.7 AT5G25220_circ_g.8
Support reads	NA
Tissues	aerial
Exon boundary	No-No "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	TT-CG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G25220.1:6 AT5G25220.2:6

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F: TTGAGCAGTCTCTTCAAAGCTTAAC
 R: AGCCATCACAGCTTCCATTG
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F: TTGAGCAGTCTCTTCAAAGCTTAAC R: AGCCATCACAGCTTCCATTG PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CGCGTAGAAACAACAAACATATGATGTGAATTGGGGAGGTGGAAGATGGGATTTGAAAGCAGGG
TTTTAGGGATTTAAAGTTGAGAATTTTATGGAGGAGgaggagcagacactatgattcagggaga
gatgaaaaccggcggtggagaaaacaaaaacgacggcggaggagctacggcggcggatggagta
gtgagctg

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							GAGGAGCAGACACTATGATTCAGGGAGAGATGAAAACCGGCGGTGGAGAAAACAAAAACGACGG
CGGAGGAGCTACGGCGGCGGATGGAGTAGTGAGCTGGCAGAATGCGAGACACAAGGCGGAGATC
CTTTCGCATCCTCTTTACGAGCAGCTTTTGTCGGCGCACGTTGCTTGTTTGAGAATCGCGACTC
CGGTTGATCAGCTTCCGAGAATCGATGCTCAGCTTGCTCAGTCTCAACACGTCGTCGCTAAATA
CTCAGCTTTAGGCGCCGCCGCTCAAGGTCTCGTCGGCGACGATAAAGAACTTGACCAGTTCATG
GTATATAAATTTCAGATTTTTTTGTTCTATAATTTTATAATTAACGATTTCATAATTATACTTA
GCACAAATTTTAATAATACACACCGACTTAGGTTTCTTGATTTTTGAAATCTTAAAATATTAAA
ATCAAGATTTGTGTTTTTAGGGATAAACAAGACTAAAATCCCAAAAAAAGAGCTTATGATATAA
TAATGGTCATGATGATGAAGAAAATACAAATTTTGTTCTTTGTGTTTATTGTGTGTTTGATTAA
TGTTTTTTTTATGGTGTAGACACATTATGTGTTGCTACTGTGTTCATTTAAAGAGCAATTGCAA
CAACATGTGCGTGTTCATGCAATGGAAGCTGTGATGGCTTGTTGGGAGATTGAGCAGTCTCTTC
AAAGCTTAACAGGTGAGAAAATGAATCCTGTGATTAGCTCATGAATCCAATAGTTAGTTTTATG
ACATTGACAAGTTTTGTGTTTTATTGGTGAGATTAGGAGTGTCTCCTGGAGAAGGGATGGGAGC
AACAATGTCTGACGATGAAGATGAACAAGTAGAGAGTGATGCTAATATGTTCGATGGGGGATTA
GATGTGTTGGGTTTTGGTCCTTTGATTCCTACTGAGAGTGAGAGGTCGTTGATGGAAAGAGTTA
GACAAGAACTTAAACATGAACTCAAACAGGTAATAATCAACAAACTTGTTCTTTGAACTAGTTC
ATGAACTAACAATGTAGAGGGCTTTGTCTTGTTGATGAAAAGGGTTACAAGGAGAAGATAGTAG
ACATAAGAGAGGAGATATTAAGGAAGAGAAGAGCTGGGAAGTTACCAGGAGATACCACCTCTGT
TCTCAAAGCTTGGTGGCAATCTCATTCCAAATGGCCTTACCCTACTGTGAGTTCACTCATCTAA
CATCTCTTTATAACTTCTTCAAGTTCATTCATTGAGCCATAACCAAATATTGGTTTGTAGGAGG
AAGATAAGGCGAGGTTGGTGCAAGAGACAGGTTTGCAGCTAAAACAGATAAACAATTGGTTCAT
CAATCAGAGAAAGAGGAACTGGCATAGCAATCCATCTTCTTCCACTGTATTGAAGAACAAACGC
AAAAGGTATTATTATACTTTAATATAGTCTTGTAACATAACCGAAAAACTTATGGATCGGACTC
AAAGAAGACTGAAACTTTTGTTTGTCTGATGAAAGCAATGCAGGTGACAATAGCGGAAGAGAGC
GGTTCGCGTAGAAACAACAAACATATGATGTGAATTGGGGAGGTGGAAGATGGGATTTGAAAGC
AGGGTTTTAGGGATTTAAAGTTGAGAATTTTATGGAGGAG
						
					

			
		

Sequences
Splice junction sequence	CGCGTAGAAACAACAAACATATGATGTGAATTGGGGAGGTGGAAGATGGGATTTGAAAGCAGGG TTTTAGGGATTTAAAGTTGAGAATTTTATGGAGGAGgaggagcagacactatgattcagggaga gatgaaaaccggcggtggagaaaacaaaaacgacggcggaggagctacggcggcggatggagta gtgagctg
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GAGGAGCAGACACTATGATTCAGGGAGAGATGAAAACCGGCGGTGGAGAAAACAAAAACGACGG CGGAGGAGCTACGGCGGCGGATGGAGTAGTGAGCTGGCAGAATGCGAGACACAAGGCGGAGATC CTTTCGCATCCTCTTTACGAGCAGCTTTTGTCGGCGCACGTTGCTTGTTTGAGAATCGCGACTC CGGTTGATCAGCTTCCGAGAATCGATGCTCAGCTTGCTCAGTCTCAACACGTCGTCGCTAAATA CTCAGCTTTAGGCGCCGCCGCTCAAGGTCTCGTCGGCGACGATAAAGAACTTGACCAGTTCATG GTATATAAATTTCAGATTTTTTTGTTCTATAATTTTATAATTAACGATTTCATAATTATACTTA GCACAAATTTTAATAATACACACCGACTTAGGTTTCTTGATTTTTGAAATCTTAAAATATTAAA ATCAAGATTTGTGTTTTTAGGGATAAACAAGACTAAAATCCCAAAAAAAGAGCTTATGATATAA TAATGGTCATGATGATGAAGAAAATACAAATTTTGTTCTTTGTGTTTATTGTGTGTTTGATTAA TGTTTTTTTTATGGTGTAGACACATTATGTGTTGCTACTGTGTTCATTTAAAGAGCAATTGCAA CAACATGTGCGTGTTCATGCAATGGAAGCTGTGATGGCTTGTTGGGAGATTGAGCAGTCTCTTC AAAGCTTAACAGGTGAGAAAATGAATCCTGTGATTAGCTCATGAATCCAATAGTTAGTTTTATG ACATTGACAAGTTTTGTGTTTTATTGGTGAGATTAGGAGTGTCTCCTGGAGAAGGGATGGGAGC AACAATGTCTGACGATGAAGATGAACAAGTAGAGAGTGATGCTAATATGTTCGATGGGGGATTA GATGTGTTGGGTTTTGGTCCTTTGATTCCTACTGAGAGTGAGAGGTCGTTGATGGAAAGAGTTA GACAAGAACTTAAACATGAACTCAAACAGGTAATAATCAACAAACTTGTTCTTTGAACTAGTTC ATGAACTAACAATGTAGAGGGCTTTGTCTTGTTGATGAAAAGGGTTACAAGGAGAAGATAGTAG ACATAAGAGAGGAGATATTAAGGAAGAGAAGAGCTGGGAAGTTACCAGGAGATACCACCTCTGT TCTCAAAGCTTGGTGGCAATCTCATTCCAAATGGCCTTACCCTACTGTGAGTTCACTCATCTAA CATCTCTTTATAACTTCTTCAAGTTCATTCATTGAGCCATAACCAAATATTGGTTTGTAGGAGG AAGATAAGGCGAGGTTGGTGCAAGAGACAGGTTTGCAGCTAAAACAGATAAACAATTGGTTCAT CAATCAGAGAAAGAGGAACTGGCATAGCAATCCATCTTCTTCCACTGTATTGAAGAACAAACGC AAAAGGTATTATTATACTTTAATATAGTCTTGTAACATAACCGAAAAACTTATGGATCGGACTC AAAGAAGACTGAAACTTTTGTTTGTCTGATGAAAGCAATGCAGGTGACAATAGCGGAAGAGAGC GGTTCGCGTAGAAACAACAAACATATGATGTGAATTGGGGAGGTGGAAGATGGGATTTGAAAGC AGGGTTTTAGGGATTTAAAGTTGAGAATTTTATGGAGGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.175128333

Conservation Information
Conserved circRNAs	NA
PMCS	0.175128333

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							ath-miR5658 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	ath-miR5658
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017;Chen et al., 2017a

circRNA

Detail information of AT5G25220_circ_g.4

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