Plant Circular RNA Database

Detail information of AT5G25220_circ_g.6

			
						 General Information
					
						CircRNA Name
						AT5G25220_circ_g.6
					
						ID in PlantcircBase
						ath_circ_040350
					
						Alias
						AT5G25220_C2, CircKNAT3-2
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 8737373-8737557  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						KNIFE, PcircRNA_finder, circRNA_finder, find_circ, CIRI2
					
						Parent gene
						
							AT5G25220						
					
						Parent gene annotation
						
							KNAT3
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT5G25220_circ_g.4 AT5G25220_circ_g.5 AT5G25220_circ_g.7 AT5G25220_circ_g.8 						
					
						Support reads
						6
					
						Tissues
						whole_plant, leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G25220.1:1
AT5G25220.2:1

General Information
CircRNA Name	AT5G25220_circ_g.6
ID in PlantcircBase	ath_circ_040350
Alias	AT5G25220_C2, CircKNAT3-2
Organism	Arabidpsis thaliana
Position	chr5: 8737373-8737557 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	KNIFE, PcircRNA_finder, circRNA_finder, find_circ, CIRI2
Parent gene	AT5G25220
Parent gene annotation	KNAT3
Parent gene strand	+
Alternative splicing	AT5G25220_circ_g.4 AT5G25220_circ_g.5 AT5G25220_circ_g.7 AT5G25220_circ_g.8
Support reads	6
Tissues	whole_plant, leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G25220.1:1 AT5G25220.2:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F:AGGAACTGGCATAGCAATCCA
 R:GCCACCAAGCTTTGAGAACAG
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F:AGGAACTGGCATAGCAATCCA R:GCCACCAAGCTTTGAGAACAG PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ATGTGTTGGGTTTTGGTCCTTTGATTCCTACTGAGAGTGAGAGGTCGTTGATGGAAAGAGTTAG
ACAAGAACTTAAACATGAACTCAAACAGgagtgtctcctggagaagggatgggagcaacaatgt
ctgacgatgaagatgaacaagtagagagtgatgctaatatgttcgatgggggattag

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 185
Transcript exons: 8737373-8737557
GAGTGTCTCCTGGAGAAGGGATGGGAGCAACAATGTCTGACGATGAAGATGAACAAGTAGAGAG
TGATGCTAATATGTTCGATGGGGGATTAGATGTGTTGGGTTTTGGTCCTTTGATTCCTACTGAG
AGTGAGAGGTCGTTGATGGAAAGAGTTAGACAAGAACTTAAACATGAACTCAAACAG
						
						Genomic sequence
						
							GAGTGTCTCCTGGAGAAGGGATGGGAGCAACAATGTCTGACGATGAAGATGAACAAGTAGAGAG
TGATGCTAATATGTTCGATGGGGGATTAGATGTGTTGGGTTTTGGTCCTTTGATTCCTACTGAG
AGTGAGAGGTCGTTGATGGAAAGAGTTAGACAAGAACTTAAACATGAACTCAAACAG

Sequences
Splice junction sequence	ATGTGTTGGGTTTTGGTCCTTTGATTCCTACTGAGAGTGAGAGGTCGTTGATGGAAAGAGTTAG ACAAGAACTTAAACATGAACTCAAACAGgagtgtctcctggagaagggatgggagcaacaatgt ctgacgatgaagatgaacaagtagagagtgatgctaatatgttcgatgggggattag
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 185 Transcript exons: 8737373-8737557 GAGTGTCTCCTGGAGAAGGGATGGGAGCAACAATGTCTGACGATGAAGATGAACAAGTAGAGAG TGATGCTAATATGTTCGATGGGGGATTAGATGTGTTGGGTTTTGGTCCTTTGATTCCTACTGAG AGTGAGAGGTCGTTGATGGAAAGAGTTAGACAAGAACTTAAACATGAACTCAAACAG
Genomic sequence	GAGTGTCTCCTGGAGAAGGGATGGGAGCAACAATGTCTGACGATGAAGATGAACAAGTAGAGAG TGATGCTAATATGTTCGATGGGGGATTAGATGTGTTGGGTTTTGGTCCTTTGATTCCTACTGAG AGTGAGAGGTCGTTGATGGAAAGAGTTAGACAAGAACTTAAACATGAACTCAAACAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.548707016

Conservation Information
Conserved circRNAs	NA
PMCS	0.548707016

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						response to drought stress

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	response to drought stress

			
						 Other Information
					
						References
						Chu et al., 2017;Zhang et al., 2019

circRNA

Detail information of AT5G25220_circ_g.6

Address

Any questions?

Supported by