Plant Circular RNA Database

Detail information of BGIOSGA003293_circ_ag.2

			
						 General Information
					
						CircRNA Name
						BGIOSGA003293_circ_ag.2
					
						ID in PlantcircBase
						osi_circ_009010
					
						Alias
						Chr11:5315929_5320224(MH63RS2), Chr11:5380629_5384925(ZS97RS2), Chr11:5315929_5320224(MH63RS2)
					
						Organism
						
							Oryza sativa ssp. indica						
					
						Position
						
							chr1: 11310819-11315115  JBrowse»						
					
						Reference genome
						
							Oryza_indica.ASM465v1.42						
					
						Type  
						
							ag-circRNA							
									
						Identification method
						CIRI2, CIRI2, CIRI2
					
						Parent gene
						
							NA						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						NA
					
						Alternative splicing
						
							BGIOSGA003293_circ_ag.1 BGIOSGA003294_circ_ag.1 BGIOSGA003294_circ_ag.2 BGIOSGA003295_circ_g.1 						
					
						Support reads
						NA
					
						Tissues
						root, leaf, panicle
					
						Exon boundary  
						
							NA-NA							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							TA-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						NA

General Information
CircRNA Name	BGIOSGA003293_circ_ag.2
ID in PlantcircBase	osi_circ_009010
Alias	Chr11:5315929_5320224(MH63RS2), Chr11:5380629_5384925(ZS97RS2), Chr11:5315929_5320224(MH63RS2)
Organism	Oryza sativa ssp. indica
Position	chr1: 11310819-11315115 JBrowse»
Reference genome	Oryza_indica.ASM465v1.42
Type	ag-circRNA
Identification method	CIRI2, CIRI2, CIRI2
Parent gene	NA
Parent gene annotation	NA
Parent gene strand	NA
Alternative splicing	BGIOSGA003293_circ_ag.1 BGIOSGA003294_circ_ag.1 BGIOSGA003294_circ_ag.2 BGIOSGA003295_circ_g.1
Support reads	NA
Tissues	root, leaf, panicle
Exon boundary	NA-NA "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	TA-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	NA

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							AATGAATACTGGTGTGTGCCCGATGATTTTAGTGCACTAACTTGCATTCTTCAACATGCACCGG
TTCTAGAGAATCTCATTCTTCAGATCTGTTCCAAGGggacctaaacatagaatgaaaatcaaag
gaaactgccattcaatggatagatcacttgtaatatcagcacaccttgagatagttgaaatcaa
atgcaaaa

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							GGACCTAAACATAGAATGAAAATCAAAGGAAACTGCCATTCAATGGATAGATCACTTGTAATAT
CAGCACACCTTGAGATAGTTGAAATCAAATGCAAAACGGTTGATAAGAGAGTACTCAAAGTTTT
GAAGTTTCTGTCTACTTTTAACATACGTAAACATTCTTCATTCTTTGTATATTTCTAAAATTTT
CCTTTACCTTAATATGCATGATATATAGCATGCTGTATTTAAGTTCTTTGGAGATGGATGCTAG
GAACATGGATTAATTGAGAAATGAAAGCATGCACTCCATTGGGTACTATTGGGAAGACGTTATC
TATTCTACTCTTTTGGCTAGTAGTATTCGAAAAAACCATTCTGATTTCTAAAATTTTCTTGCAT
CGCGTAACCGGTATGATAACATGTATGCCAGTATGTGTGTAATTATATCTTTCAATAATTTTAT
TTGATTTTCTTTCCCATCAGTTGCCATCTTGAATTCCAGTTTATTAGTTGCTCTTATAGCTCGC
ATGCCATTTGCCATGTATGCAAGTACCTCACAGACTTTGTTCTGCTATGATAATCAGTTTACCA
TTACCAGAGGTGCTGTATGCAAGTTTGCTGCACTAGTCATCCAGGTTGTATCATAGCAGCTAGT
AATTAATAATTGGGCAAGATTGCTTAGTTGTGTTGTTTGGCTAAGGTAATTTTTTTTTTGTCAC
ACTAGATTATATTTGTTATGTTGAGCTCACAACGCCTGGTGATTATAGCCTGGTGTCCAAAAAT
CTTGTTTACAAATTGCCATGGGTACTCACATAGTCCCTAGACCATGCAATTTTCTCTCTTGCCG
TGTGCTAGGTTGTAGAGAATCCAACTACTATGCATTATAGGATGCAACCATGTTGTAAAGGACC
AATGCTTGTTCAAGTGAGGGCATTTTATTTTGTTTCTTTTCTTTAAATGTAGTGGATACAATCA
GATTTTAGTCATGAAGAAGTAATGAAGTCTTTATGTCCATTTGGATTTTGAAGGATTGGTCCAA
TGGACTAAGTTTGTCAATGGATTTGAAGTTTTTGTTTTGAAGTGAAGTTTCAGGTGAGACCTCT
TGAAACTTACTGTGCTCTTCTTATTTGTTTTTGTGCAGTTTTTAGTTTTGAGGAAATCGAGATT
TTGGAAGAAGATGAAAATGACCAGGATGAAGAGGAGGAAGATGAGGATGAAGACGAGGACTCTT
ATGAAAACCCTTTTGAAGACGAAAATGAAGACTCTTACAAAGGCGAACATGAAGACGAAGACAA
TGAAGACGAAGACGAAGACGATGACGATGAAGACGAAGACGAAGACGAAGATGAAGAGTAAGAG
GAAGAGGAAGAGAAAAATGGTTGATGCACCACTGTTTTGATTTATTAAGAAGGTTAAGCTTCCA
TGTGCTCACTACCTGGGCTTCCGAGCAGTGGTGGACGCAACTTCAAAATATAGATGTGGCGGAG
AAAAGAGATGTTCACTCGATAAGAGCAATTAAACACAACACATATCGTATTTATAGTTCAATCG
CATATAACTGTCTCAATCATTGTTTGCGGCGTGCGTATGTTGTGAAGAAACGAACATCTATTGA
GTGCTTACACCTTCTTATACCTAATTTTGATGGTCCACAACTATTATAAGGGGTTAGGGGGGGT
TTGAGGGTGTGGGGCAATACGAGTCGTAGCTGGGACAGTCGACCTACCCTGCTTAATGGGTAGA
TCCGCCCCTGCTTCCGAGGGTTTTTTAATTCATAGGAAAAACATAAAAAGTTTAAGGATTTTAA
TTCTGTAGAAAAAATTCTGAAATGACCTTTAAAACAAATGGTTAAATCAATTTGTTTTATTTAT
GAAGACTTTGAAATTCTATGAAATGTACAATCATATAAAGACTTTGGAGGAAAGTTACCAAGAG
CTCTAACGTTTTTGGAATTTTAGTTTAAGTCTATCTCTCATCCATCTTGTATCTTATCCTGCTA
AACGATTATTCTTATATTTTTTTTATTTTGTAATCATCTGTCTTGTAATTTATTTTTATCTGTT
ATATTCAGATTCGTAGTAGTATGATATGTCACATCCTGTATTAGAGTGCATTTATATAGGGACA
GAGGAAATATATTTTAAATGAGCTCTAAACTGAGAGCCCCATCAATTAGCCTAATAAGTAAAAG
CAAAAGCCAAAATTTAAATTTTTAACTTGATTTTAAGATATTTCAACGTAATTTCTTTTTAGCA
TTGCCCTTTAAATCGCTATGAACCAATATATAAAAGTTTTATCTAGAAACTAATTTTTATTTCC
CAATAACTTATTTTGGCTTATTAAGCTGCGTTCGTTAGTCCAGGTTCGGAACTCTCCTGTCTCG
ATTTCCGTGCGTACGCTTTTGAAACTGTTAAACGGTGCGCTTTTTTTAAAAAAAGTTTCTGTAC
GAAAGTTGTTTAAAAAATCATATTGATTCTTTTTGAAAAAAATTAGCTAATACTTAATTAATCA
CGCGCTAATGGACCACTCCGTTTTCTGTGCTGGAGGGCAGCATTGGACTACCGAACACAACCTA
AGGCTATAAGCTTGATATGAAATAATTATTTTATATAAGTTGCTTAAAAATATTAGATAAATTT
ATTTGTAAGTTTATAATAATGAAAACTCAATTAATAATGCGGTAATATTTTTTCCCGCGTCCTA
ACTTTATTTTCATGCTAGTAACAAAAAAATAAAATAAAACTATATGCACAGCAAACTGACTCCC
AGGCTCTCCCCAGTCATGACTCACCTCAGTAGAGATAGAAACTGACTACAAACTCTCCGTCTTC
TCCACCCAGCACAGCATCCCACCCTTTCCTCCCCTCCGATCCAGAGCTCTCCGTCCGCTTCCCG
CGCTCGTGTCGCGCTTCTCCTCTCCAGCGTCGCGCCGCGCCATTGGCGTATCCGTATCCTTGCA
ACGCGGGCGCCGCGCGTTCCACTCTCTGGTCGGTCTCCGGTGGCCGGGGGGAGGCTGCCGGTGG
CGATGTGGGCAGCTTGGTCCTGACCCTTACAGCCCTCGCTCCTGGTGTTACTGTTACCTGAGCT
GTTCTTGCTACGCTGGGAGGGGAGGGGTGGCGCCGCGGCGACGCCCGCAACCTGTTCGACGGAA
TGTCTCCGCGGAAGATGCCCAAGGAATCGCCTGGCTCGACCGGGGGAGACCGCATAGGGGACCT
CCCGGATGAGGTGCTCCACCACGTGCTCTCCTTCCTACCGGCGCAGGAGGCTGTCCGGACGTGC
CTGCTGGCTCGGCGATGGCTCCACCTCTGGAAGTCCGCCACGGGGCTGCGCATTGGCGAGGACT
GCGACTATCTGGGAAGCGTGAAGACACAGAAGGAGTTTCTCGACCGTCTGCTGCTACTCCGTGA
TGGAGCCCCTCTCGACACGTGCGTGCTAATGTTCGATTATTACGGCGACATGGACATCGAGGAC
ACAGCTCGGGTGAACCTATGGTTCAGGCATGCTCTTATCCACAAGGCTCGGTTCCTCTGGCTCG
ACGTCGGATACTACCACTCATTTGTGATAGATGAGATGCCACTTGTCTCCCAGCACTTGACGAG
GCTACAGCTTCGAAATATAAGGGTAAACGACAGCTTCCTTAACTTCTCGAGCTGTCCAGCATTG
GAACATCTAGTGTTTCAGTCATGTAAATTTGGCTGTGCCAAGATCTCATCTAGATCTGCAAAAC
GTTTGAGCATAACCAATTCCTATTTCAGTGAGATATCTCGGGTACGTATCGCTATCCCGAGTCT
TGTTTCTCTGCAGCTGGATGGCTTCCATGACAGGGCTCCAGTGCTTGAGAGGATGCCATCATTA
GTTGATGCATTTGTTGGAGTCCTCAACTGGACTAAAGACTATTGCATTTGGTCTGACTCTGGGG
ATTGTGGTCATGAGAACTGCGAATCTTGTTACGGTATCAAGGACAACAATTGTGTGCTTTTGGA
AGGTTTATCGGAAGCTAAGACGTTGGGGCTGATAAGTGAACGTGGATCGGTATGCTTGCACTTC
CATCTTTAAGTTGTTTGTATTATCTCATTATTTATATTAATGTGCTTATATCCACTTGAACTCT
GTGGTTTTTGTTTATCTTCTTTTTTGCACTCCGCGCAGTTTATTTTGAAAAGGGATTTGAAATG
GTGCCCAACATTTACCAAGCTAAAGACTTTGTTACTCAATGAATACTGGTGTGTGCCCGATGAT
TTTAGTGCACTAACTTGCATTCTTCAACATGCACCGGTTCTAGAGAATCTCATTCTTCAGATCT
GTTCCAAGG
						
					

			
		

Sequences
Splice junction sequence	AATGAATACTGGTGTGTGCCCGATGATTTTAGTGCACTAACTTGCATTCTTCAACATGCACCGG TTCTAGAGAATCTCATTCTTCAGATCTGTTCCAAGGggacctaaacatagaatgaaaatcaaag gaaactgccattcaatggatagatcacttgtaatatcagcacaccttgagatagttgaaatcaa atgcaaaa
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GGACCTAAACATAGAATGAAAATCAAAGGAAACTGCCATTCAATGGATAGATCACTTGTAATAT CAGCACACCTTGAGATAGTTGAAATCAAATGCAAAACGGTTGATAAGAGAGTACTCAAAGTTTT GAAGTTTCTGTCTACTTTTAACATACGTAAACATTCTTCATTCTTTGTATATTTCTAAAATTTT CCTTTACCTTAATATGCATGATATATAGCATGCTGTATTTAAGTTCTTTGGAGATGGATGCTAG GAACATGGATTAATTGAGAAATGAAAGCATGCACTCCATTGGGTACTATTGGGAAGACGTTATC TATTCTACTCTTTTGGCTAGTAGTATTCGAAAAAACCATTCTGATTTCTAAAATTTTCTTGCAT CGCGTAACCGGTATGATAACATGTATGCCAGTATGTGTGTAATTATATCTTTCAATAATTTTAT TTGATTTTCTTTCCCATCAGTTGCCATCTTGAATTCCAGTTTATTAGTTGCTCTTATAGCTCGC ATGCCATTTGCCATGTATGCAAGTACCTCACAGACTTTGTTCTGCTATGATAATCAGTTTACCA TTACCAGAGGTGCTGTATGCAAGTTTGCTGCACTAGTCATCCAGGTTGTATCATAGCAGCTAGT AATTAATAATTGGGCAAGATTGCTTAGTTGTGTTGTTTGGCTAAGGTAATTTTTTTTTTGTCAC ACTAGATTATATTTGTTATGTTGAGCTCACAACGCCTGGTGATTATAGCCTGGTGTCCAAAAAT CTTGTTTACAAATTGCCATGGGTACTCACATAGTCCCTAGACCATGCAATTTTCTCTCTTGCCG TGTGCTAGGTTGTAGAGAATCCAACTACTATGCATTATAGGATGCAACCATGTTGTAAAGGACC AATGCTTGTTCAAGTGAGGGCATTTTATTTTGTTTCTTTTCTTTAAATGTAGTGGATACAATCA GATTTTAGTCATGAAGAAGTAATGAAGTCTTTATGTCCATTTGGATTTTGAAGGATTGGTCCAA TGGACTAAGTTTGTCAATGGATTTGAAGTTTTTGTTTTGAAGTGAAGTTTCAGGTGAGACCTCT TGAAACTTACTGTGCTCTTCTTATTTGTTTTTGTGCAGTTTTTAGTTTTGAGGAAATCGAGATT TTGGAAGAAGATGAAAATGACCAGGATGAAGAGGAGGAAGATGAGGATGAAGACGAGGACTCTT ATGAAAACCCTTTTGAAGACGAAAATGAAGACTCTTACAAAGGCGAACATGAAGACGAAGACAA TGAAGACGAAGACGAAGACGATGACGATGAAGACGAAGACGAAGACGAAGATGAAGAGTAAGAG GAAGAGGAAGAGAAAAATGGTTGATGCACCACTGTTTTGATTTATTAAGAAGGTTAAGCTTCCA TGTGCTCACTACCTGGGCTTCCGAGCAGTGGTGGACGCAACTTCAAAATATAGATGTGGCGGAG AAAAGAGATGTTCACTCGATAAGAGCAATTAAACACAACACATATCGTATTTATAGTTCAATCG CATATAACTGTCTCAATCATTGTTTGCGGCGTGCGTATGTTGTGAAGAAACGAACATCTATTGA GTGCTTACACCTTCTTATACCTAATTTTGATGGTCCACAACTATTATAAGGGGTTAGGGGGGGT TTGAGGGTGTGGGGCAATACGAGTCGTAGCTGGGACAGTCGACCTACCCTGCTTAATGGGTAGA TCCGCCCCTGCTTCCGAGGGTTTTTTAATTCATAGGAAAAACATAAAAAGTTTAAGGATTTTAA TTCTGTAGAAAAAATTCTGAAATGACCTTTAAAACAAATGGTTAAATCAATTTGTTTTATTTAT GAAGACTTTGAAATTCTATGAAATGTACAATCATATAAAGACTTTGGAGGAAAGTTACCAAGAG CTCTAACGTTTTTGGAATTTTAGTTTAAGTCTATCTCTCATCCATCTTGTATCTTATCCTGCTA AACGATTATTCTTATATTTTTTTTATTTTGTAATCATCTGTCTTGTAATTTATTTTTATCTGTT ATATTCAGATTCGTAGTAGTATGATATGTCACATCCTGTATTAGAGTGCATTTATATAGGGACA GAGGAAATATATTTTAAATGAGCTCTAAACTGAGAGCCCCATCAATTAGCCTAATAAGTAAAAG CAAAAGCCAAAATTTAAATTTTTAACTTGATTTTAAGATATTTCAACGTAATTTCTTTTTAGCA TTGCCCTTTAAATCGCTATGAACCAATATATAAAAGTTTTATCTAGAAACTAATTTTTATTTCC CAATAACTTATTTTGGCTTATTAAGCTGCGTTCGTTAGTCCAGGTTCGGAACTCTCCTGTCTCG ATTTCCGTGCGTACGCTTTTGAAACTGTTAAACGGTGCGCTTTTTTTAAAAAAAGTTTCTGTAC GAAAGTTGTTTAAAAAATCATATTGATTCTTTTTGAAAAAAATTAGCTAATACTTAATTAATCA CGCGCTAATGGACCACTCCGTTTTCTGTGCTGGAGGGCAGCATTGGACTACCGAACACAACCTA AGGCTATAAGCTTGATATGAAATAATTATTTTATATAAGTTGCTTAAAAATATTAGATAAATTT ATTTGTAAGTTTATAATAATGAAAACTCAATTAATAATGCGGTAATATTTTTTCCCGCGTCCTA ACTTTATTTTCATGCTAGTAACAAAAAAATAAAATAAAACTATATGCACAGCAAACTGACTCCC AGGCTCTCCCCAGTCATGACTCACCTCAGTAGAGATAGAAACTGACTACAAACTCTCCGTCTTC TCCACCCAGCACAGCATCCCACCCTTTCCTCCCCTCCGATCCAGAGCTCTCCGTCCGCTTCCCG CGCTCGTGTCGCGCTTCTCCTCTCCAGCGTCGCGCCGCGCCATTGGCGTATCCGTATCCTTGCA ACGCGGGCGCCGCGCGTTCCACTCTCTGGTCGGTCTCCGGTGGCCGGGGGGAGGCTGCCGGTGG CGATGTGGGCAGCTTGGTCCTGACCCTTACAGCCCTCGCTCCTGGTGTTACTGTTACCTGAGCT GTTCTTGCTACGCTGGGAGGGGAGGGGTGGCGCCGCGGCGACGCCCGCAACCTGTTCGACGGAA TGTCTCCGCGGAAGATGCCCAAGGAATCGCCTGGCTCGACCGGGGGAGACCGCATAGGGGACCT CCCGGATGAGGTGCTCCACCACGTGCTCTCCTTCCTACCGGCGCAGGAGGCTGTCCGGACGTGC CTGCTGGCTCGGCGATGGCTCCACCTCTGGAAGTCCGCCACGGGGCTGCGCATTGGCGAGGACT GCGACTATCTGGGAAGCGTGAAGACACAGAAGGAGTTTCTCGACCGTCTGCTGCTACTCCGTGA TGGAGCCCCTCTCGACACGTGCGTGCTAATGTTCGATTATTACGGCGACATGGACATCGAGGAC ACAGCTCGGGTGAACCTATGGTTCAGGCATGCTCTTATCCACAAGGCTCGGTTCCTCTGGCTCG ACGTCGGATACTACCACTCATTTGTGATAGATGAGATGCCACTTGTCTCCCAGCACTTGACGAG GCTACAGCTTCGAAATATAAGGGTAAACGACAGCTTCCTTAACTTCTCGAGCTGTCCAGCATTG GAACATCTAGTGTTTCAGTCATGTAAATTTGGCTGTGCCAAGATCTCATCTAGATCTGCAAAAC GTTTGAGCATAACCAATTCCTATTTCAGTGAGATATCTCGGGTACGTATCGCTATCCCGAGTCT TGTTTCTCTGCAGCTGGATGGCTTCCATGACAGGGCTCCAGTGCTTGAGAGGATGCCATCATTA GTTGATGCATTTGTTGGAGTCCTCAACTGGACTAAAGACTATTGCATTTGGTCTGACTCTGGGG ATTGTGGTCATGAGAACTGCGAATCTTGTTACGGTATCAAGGACAACAATTGTGTGCTTTTGGA AGGTTTATCGGAAGCTAAGACGTTGGGGCTGATAAGTGAACGTGGATCGGTATGCTTGCACTTC CATCTTTAAGTTGTTTGTATTATCTCATTATTTATATTAATGTGCTTATATCCACTTGAACTCT GTGGTTTTTGTTTATCTTCTTTTTTGCACTCCGCGCAGTTTATTTTGAAAAGGGATTTGAAATG GTGCCCAACATTTACCAAGCTAAAGACTTTGTTACTCAATGAATACTGGTGTGTGCCCGATGAT TTTAGTGCACTAACTTGCATTCTTCAACATGCACCGGTTCTAGAGAATCTCATTCTTCAGATCT GTTCCAAGG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osa_circ_008779 						
					
						PMCS

Conservation Information
Conserved circRNAs	osa_circ_008779
PMCS

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							osa-miR816 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	osa-miR816
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhou et al., 2021

circRNA

Detail information of BGIOSGA003293_circ_ag.2

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