Plant Circular RNA Database

Detail information of Os10g0320400_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os10g0320400_circ_g.4
					
						ID in PlantcircBase
						osa_circ_006455
					
						Alias
						Os_ciR1164
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr10: 8693803-8694123  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, PcircRNA_finder, find_circ
					
						Parent gene
						
							Os10g0320400						
					
						Parent gene annotation
						
							Similar to ATP synthase gamma chain, mitochondrial precursor (EC
 3.6.3.14). (Os10t0320400-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os10g0320400_circ_igg.1 Os10g0320400_circ_g.1 Os10g0320400_circ_g.2 Os10g0320400_circ_g.3 Os10g0320400_circ_g.5 Os10g0320400_circ_g.6 Os10g0320400_circ_g.7 Os10g0320400_circ_g.8 						
					
						Support reads
						15/22
					
						Tissues
						root/shoot, root, seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os10t0320400-01:2

General Information
CircRNA Name	Os10g0320400_circ_g.4
ID in PlantcircBase	osa_circ_006455
Alias	Os_ciR1164
Organism	Oryza sativa
Position	chr10: 8693803-8694123 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, PcircRNA_finder, find_circ
Parent gene	Os10g0320400
Parent gene annotation	Similar to ATP synthase gamma chain, mitochondrial precursor (EC 3.6.3.14). (Os10t0320400-01)
Parent gene strand	+
Alternative splicing	Os10g0320400_circ_igg.1 Os10g0320400_circ_g.1 Os10g0320400_circ_g.2 Os10g0320400_circ_g.3 Os10g0320400_circ_g.5 Os10g0320400_circ_g.6 Os10g0320400_circ_g.7 Os10g0320400_circ_g.8
Support reads	15/22
Tissues	root/shoot, root, seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os10t0320400-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ATTAGGAGAAAAGGGTAAAGTTCAACTCATTCGTGACTCCAAGGATAACATCGAGATGACAGTA
TCTGAGTTGCAGAAGAACCCTATCAACTACACACAGgtgtcgatgtcaagaagaatgttattgt
agccattacctctgacaagggtctttgtggtggtatcaactcaacgtcagttaaagtcagcaaa
gcactcca

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 242
Transcript exons: 8693803-8693916,8693996-8694123
GTGTCGATGTCAAGAAGAATGTTATTGTAGCCATTACCTCTGACAAGGGTCTTTGTGGTGGTAT
CAACTCAACGTCAGTTAAAGTCAGCAAAGCACTCCACAAGTTGACTTCTGGACCAGAGAAAGAA
AGCAAGTATGTCATATTAGGAGAAAAGGGTAAAGTTCAACTCATTCGTGACTCCAAGGATAACA
TCGAGATGACAGTATCTGAGTTGCAGAAGAACCCTATCAACTACACACAG
						
						Genomic sequence
						
							GTGTCGATGTCAAGAAGAATGTTATTGTAGCCATTACCTCTGACAAGGGTCTTTGTGGTGGTAT
CAACTCAACGTCAGTTAAAGTCAGCAAAGCACTCCACAAGTTGACTTCTGGTAATACTTTGCAT
GGTTATTTTTGTTATCATTTAAACATTAGAAGATACTAAAACAAAGTTTTTGCACCTCAAATCA
GGACCAGAGAAAGAAAGCAAGTATGTCATATTAGGAGAAAAGGGTAAAGTTCAACTCATTCGTG
ACTCCAAGGATAACATCGAGATGACAGTATCTGAGTTGCAGAAGAACCCTATCAACTACACACA
G

Sequences
Splice junction sequence	ATTAGGAGAAAAGGGTAAAGTTCAACTCATTCGTGACTCCAAGGATAACATCGAGATGACAGTA TCTGAGTTGCAGAAGAACCCTATCAACTACACACAGgtgtcgatgtcaagaagaatgttattgt agccattacctctgacaagggtctttgtggtggtatcaactcaacgtcagttaaagtcagcaaa gcactcca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 242 Transcript exons: 8693803-8693916,8693996-8694123 GTGTCGATGTCAAGAAGAATGTTATTGTAGCCATTACCTCTGACAAGGGTCTTTGTGGTGGTAT CAACTCAACGTCAGTTAAAGTCAGCAAAGCACTCCACAAGTTGACTTCTGGACCAGAGAAAGAA AGCAAGTATGTCATATTAGGAGAAAAGGGTAAAGTTCAACTCATTCGTGACTCCAAGGATAACA TCGAGATGACAGTATCTGAGTTGCAGAAGAACCCTATCAACTACACACAG
Genomic sequence	GTGTCGATGTCAAGAAGAATGTTATTGTAGCCATTACCTCTGACAAGGGTCTTTGTGGTGGTAT CAACTCAACGTCAGTTAAAGTCAGCAAAGCACTCCACAAGTTGACTTCTGGTAATACTTTGCAT GGTTATTTTTGTTATCATTTAAACATTAGAAGATACTAAAACAAAGTTTTTGCACCTCAAATCA GGACCAGAGAAAGAAAGCAAGTATGTCATATTAGGAGAAAAGGGTAAAGTTCAACTCATTCGTG ACTCCAAGGATAACATCGAGATGACAGTATCTGAGTTGCAGAAGAACCCTATCAACTACACACA G

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_002772* osi_circ_008854 						
					
						PMCS
						0.558853946

Conservation Information
Conserved circRNAs	osi_circ_002772* osi_circ_008854
PMCS	0.558853946

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							8694080-8693829(+)
8694025-8694112(-)
						
						Potential amino acid sequence
						
							MTVSELQKNPINYTQVSMSRRMLL*(+)
MTYLLSFSGPEVNLWSALLTLTDVELIPPQRPLSEVMATITFFLTSTPVCS*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	8694080-8693829(+) 8694025-8694112(-)
Potential amino acid sequence	MTVSELQKNPINYTQVSMSRRMLL(+) MTYLLSFSGPEVNLWSALLTLTDVELIPPQRPLSEVMATITFFLTSTPVCS(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os10g0320400_circ_g.4

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