Plant Circular RNA Database

Detail information of Os12g0264500_circ_g.27

			
						 General Information
					
						CircRNA Name
						Os12g0264500_circ_g.27
					
						ID in PlantcircBase
						osa_circ_011077
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr12: 9361824-9362062  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder
					
						Parent gene
						
							Os12g0264500						
					
						Parent gene annotation
						
							Similar to CoA-thioester hydrolase CHY1 (3-hydroxyisobutyryl-coe
nzyme A hydrolase). (Os12t0264500-01);Similar to CHY1. (Os12t026
4500-02);Conserved hypothetical protein. (Os12t0264500-03);Simil
ar to cDNA clone:J013000A22, full insert sequence. (Os12t0264500
-04)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os12g0264500_circ_igg.1 Os12g0264500_circ_igg.2 Os12g0264500_circ_g.1 Os12g0264500_circ_g.2 Os12g0264500_circ_g.3 Os12g0264500_circ_g.4 Os12g0264500_circ_g.5 Os12g0264500_circ_g.6 Os12g0264500_circ_g.7 Os12g0264500_circ_g.8 Os12g0264500_circ_g.9 Os12g0264500_circ_g.10 Os12g0264500_circ_g.11 Os12g0264500_circ_g.12 Os12g0264500_circ_g.13 Os12g0264500_circ_g.14 Os12g0264500_circ_g.15 Os12g0264500_circ_g.16 Os12g0264500_circ_g.17 Os12g0264500_circ_g.18 Os12g0264500_circ_g.19 Os12g0264500_circ_g.20 Os12g0264500_circ_g.21 Os12g0264500_circ_g.22 Os12g0264500_circ_g.23 Os12g0264500_circ_g.24 Os12g0264500_circ_g.25 Os12g0264500_circ_g.26 						
					
						Support reads
						2
					
						Tissues
						seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os12t0264500-02:2
Os12t0264500-03:2
Os12t0264500-01:2

General Information
CircRNA Name	Os12g0264500_circ_g.27
ID in PlantcircBase	osa_circ_011077
Alias	NA
Organism	Oryza sativa
Position	chr12: 9361824-9362062 JBrowse»
Reference genome	IRGSP-1.0.38
Type	u-circRNA
Identification method	CIRCexplorer, PcircRNA_finder
Parent gene	Os12g0264500
Parent gene annotation	Similar to CoA-thioester hydrolase CHY1 (3-hydroxyisobutyryl-coe nzyme A hydrolase). (Os12t0264500-01);Similar to CHY1. (Os12t026 4500-02);Conserved hypothetical protein. (Os12t0264500-03);Simil ar to cDNA clone:J013000A22, full insert sequence. (Os12t0264500 -04)
Parent gene strand	+
Alternative splicing	Os12g0264500_circ_igg.1 Os12g0264500_circ_igg.2 Os12g0264500_circ_g.1 Os12g0264500_circ_g.2 Os12g0264500_circ_g.3 Os12g0264500_circ_g.4 Os12g0264500_circ_g.5 Os12g0264500_circ_g.6 Os12g0264500_circ_g.7 Os12g0264500_circ_g.8 Os12g0264500_circ_g.9 Os12g0264500_circ_g.10 Os12g0264500_circ_g.11 Os12g0264500_circ_g.12 Os12g0264500_circ_g.13 Os12g0264500_circ_g.14 Os12g0264500_circ_g.15 Os12g0264500_circ_g.16 Os12g0264500_circ_g.17 Os12g0264500_circ_g.18 Os12g0264500_circ_g.19 Os12g0264500_circ_g.20 Os12g0264500_circ_g.21 Os12g0264500_circ_g.22 Os12g0264500_circ_g.23 Os12g0264500_circ_g.24 Os12g0264500_circ_g.25 Os12g0264500_circ_g.26
Support reads	2
Tissues	seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os12t0264500-02:2 Os12t0264500-03:2 Os12t0264500-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GGAACAAGAGGCTCCAAATGTTGCTGATGAATGGATTGCTTCTGCAATCCAGTCCATGAGGAAG
GCTTCTCCTACTAGTATGAAAATATCCTTGAGATCGgctagaaatcatcaacaaatgcttttcc
aaaaaaacagtagaagaaatcatagcctctcttgtgagttatgctaagtcctcatttgctttct
atggactc

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GCTAGAAATCATCAACAAATGCTTTTCCAAAAAAACAGTAGAAGAAATCATAGCCTCTCTTGTG
AGTTATGCTAAGTCCTCATTTGCTTTCTATGGACTCTCCAGTTCTGTTGTGCTAAATTTGATGT
GTATCATGTTAGGAACAAGAGGCTCCAAATGTTGCTGATGAATGGATTGCTTCTGCAATCCAGT
CCATGAGGAAGGCTTCTCCTACTAGTATGAAAATATCCTTGAGATCG

Sequences
Splice junction sequence	GGAACAAGAGGCTCCAAATGTTGCTGATGAATGGATTGCTTCTGCAATCCAGTCCATGAGGAAG GCTTCTCCTACTAGTATGAAAATATCCTTGAGATCGgctagaaatcatcaacaaatgcttttcc aaaaaaacagtagaagaaatcatagcctctcttgtgagttatgctaagtcctcatttgctttct atggactc
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GCTAGAAATCATCAACAAATGCTTTTCCAAAAAAACAGTAGAAGAAATCATAGCCTCTCTTGTG AGTTATGCTAAGTCCTCATTTGCTTTCTATGGACTCTCCAGTTCTGTTGTGCTAAATTTGATGT GTATCATGTTAGGAACAAGAGGCTCCAAATGTTGCTGATGAATGGATTGCTTCTGCAATCCAGT CCATGAGGAAGGCTTCTCCTACTAGTATGAAAATATCCTTGAGATCG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.20382228

Conservation Information
Conserved circRNAs	NA
PMCS	0.20382228

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							9362019-9361883(+)
9362021-9361827(-)
						
						Potential amino acid sequence
						
							MRKASPTSMKISLRSARNHQQMLFQKNSRRNHSLS*(+)
MDWIAEAIHSSATFGASCSREAMISSTVFLEKHLLMISSRSQGYFHTSRRSLPHGLDCRSNPFI
SNIWSLLFKRGYDFFYCFFGKAFVDDF*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	9362019-9361883(+) 9362021-9361827(-)
Potential amino acid sequence	MRKASPTSMKISLRSARNHQQMLFQKNSRRNHSLS(+) MDWIAEAIHSSATFGASCSREAMISSTVFLEKHLLMISSRSQGYFHTSRRSLPHGLDCRSNPFI SNIWSLLFKRGYDFFYCFFGKAFVDDF(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os12g0264500_circ_g.27

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