Plant Circular RNA Database

Detail information of Os08g0223766_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os08g0223766_circ_g.4
					
						ID in PlantcircBase
						osa_circ_036380
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr8: 7497952-7499855  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os08g0223766						
					
						Parent gene annotation
						
							Similar to H0702G05.10 protein. (Os08t0223766-00)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os08g0223766_circ_igg.1 Os08g0223766_circ_igg.2 Os08g0223766_circ_g.1 Os08g0223766_circ_g.2 Os08g0223766_circ_g.3 Os08g0223766_circ_g.5 Os08g0223766_circ_g.6 Os08g0223766_circ_ag.1 Os08g0223833_circ_g.1 Os08g0223833_circ_g.2 						
					
						Support reads
						2
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os08t0223766-00:6

General Information
CircRNA Name	Os08g0223766_circ_g.4
ID in PlantcircBase	osa_circ_036380
Alias	NA
Organism	Oryza sativa
Position	chr8: 7497952-7499855 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os08g0223766
Parent gene annotation	Similar to H0702G05.10 protein. (Os08t0223766-00)
Parent gene strand	+
Alternative splicing	Os08g0223766_circ_igg.1 Os08g0223766_circ_igg.2 Os08g0223766_circ_g.1 Os08g0223766_circ_g.2 Os08g0223766_circ_g.3 Os08g0223766_circ_g.5 Os08g0223766_circ_g.6 Os08g0223766_circ_ag.1 Os08g0223833_circ_g.1 Os08g0223833_circ_g.2
Support reads	2
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os08t0223766-00:6

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							AAGCGGCACTATGGACCTGATGTATGCTTTAAAGGACAAGCTAGGAAACTCGCAAAAATGGAAG
ATGAAAGTGATGATGGAACTGATGAATATTCTCTACgtgctcttgagaaaggtggtgtgggtgc
tcttgaacttgttgctatggacatgaaagccaggttagctcttcctggaacttctcggtggttc
ggttctac

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 633
Transcript exons: 7497952-7498012,7498221-7498284,7498485-7498613,7498852-
7498995,7499444-7499546,7499724-7499855
GTGCTCTTGAGAAAGGTGGTGTGGGTGCTCTTGAACTTGTTGCTATGGACATGAAAGCCAGAAC
GCTAAGTTATAAGGGTGCTGCTTTTGCTACCGTGGAAGCTCCTCTAGAGGAAAGAATGATGAAT
ATGTATAGGAAAGCAGCTGAATTTTGGGCCGAGTTGCGTGTTGAACTCCTTTCAGCAATTGAAT
ATTATGCAGAAGACAAGGGCAATTCATCTCAGATTTGGCGGTTATACTGGGCCAGCCATCAGCG
TTTCTTTAGGCATATGTGTATGTCGGCTAAAGTACCTGCTGTTGTGAGATTAGTGAAAGAAGCC
TTAGCAGAAGAAAAATGTGTGGTGATTGGTCTTCAAAGCACTGGAGAAGCTCGAACAGAGGAAG
CTATCAGTAAATATGGTGTTGAAATGGAAGACTTTGTTTCTGGTCCAAGGGAGTTGCTTCTTAA
GCTGGTAGATGACAATTATCCTTTGCCTCCGAAACCTGATTGCTTTCAACAAGTAGGTGATGAA
AAAGTTGCAGAGGTTCAGCGTAAGCGGCACTATGGACCTGATGTATGCTTTAAAGGACAAGCTA
GGAAACTCGCAAAAATGGAAGATGAAAGTGATGATGGAACTGATGAATATTCTCTAC
						
					

						Genomic sequence
						
							GTGCTCTTGAGAAAGGTGGTGTGGGTGCTCTTGAACTTGTTGCTATGGACATGAAAGCCAGGTT
AGCTCTTCCTGGAACTTCTCGGTGGTTCGGTTCTACCTTTTACACACATCGTAGTTGACATGAA
GAGCCCTTATGCATTGGGTGTATATACAAATTGCTCTTTCTGTACATAGGTAAGCCAATTACCT
CGTTGATACATTTCCTCTTCTAATAATGATTGGTATACCTCTTGTTCAACTTGTCCCAGGGGTA
TGTATGTTTGCAGAACGCTAAGTTATAAGGGTGCTGCTTTTGCTACCGTGGAAGCTCCTCTAGA
GGAAAGAATGATGGTAAGATTGATGCTCCTTATTTTCTGCTTTTATTTTGGTGGTATGATTAGT
TTACTTTGAACCATTTTAAAAATCTTTATAGTTTACAATGTGATTTCACATAACATTAAAAACC
AATGTTATGTTTAGCCTTTTCTAATCATTTTTTGTGTAGTGCTGGTGTTGATTTTTATTTCACA
TTTTGTTATCTCATGATGCAGAATATGTATAGGAAAGCAGCTGAATTTTGGGCCGAGTTGCGTG
TTGAACTCCTTTCAGCAATTGAATATTATGCAGAAGACAAGGGCAATTCATCTCAGATTTGGCG
GTTATACTGGGCCAGCCATCAGGTGGTTCTCTTTCATTTTACGTAACAATATGTTTATAACCAT
AACACCCTAGCCAATTATGAGATTATGGTTCCCCAGAAATCATATTGCTTCCTTTTGGTATAAT
ATATGATCTTTGATTGGGGATTAAATTCTGATTCAGTGGATTTGAACATGGCAATTTGTTTGAC
CTTCTGAAAAACTACTACATATGATTAATAACTAAATAACAGTATGCTATGTATATATATTTTT
GCAGCGTTTCTTTAGGCATATGTGTATGTCGGCTAAAGTACCTGCTGTTGTGAGATTAGTGAAA
GAAGCCTTAGCAGAAGAAAAATGTGTGGTGATTGGTCTTCAAAGCACTGGAGAAGCTCGAACAG
AGGAAGCTATCAGTAAATATGTGTGTTTTTCATGCATAGCACTTTCATCCTGTATACCTAAAGA
GTGAACTTCACAATACTACAACTTTTTTTTTTTTGTCAAAACCATCTCTTCGCTGCAACTTTAG
CCAGACATTTCACAAAACTGCAATCATAGCACAATATTTTGTCAGAAAATCACCACTGTCCCTT
GTGTGTATTTAACATGAGTGTCCAACTTGTAGAAGTGAGTCTGACTAGTGGGCCTGACCTATCG
GACAGGCCCTTTCTGACGAAGTTGCAGTTATTTTTTGGTTAAATCATCAGTTGACTTTTCTGTT
TTCTGAAATGTTCAACAAAAATTGCAGCAAACCGATGGTTTTGATGAGCAAAATAGTTGCAATT
CAGTGAAATTTACTCTATTCTAAAACCTATTTTGGTGAATTGATTCTTGGAATTATTTTCCTGT
TTACTGAATGAATGTGGTAGGGTGTTGAAATGGAAGACTTTGTTTCTGGTCCAAGGGAGTTGCT
TCTTAAGCTGGTAGATGACAATTATCCTTTGCCTCCGAAACCTGATTGCTTTCAACAAGGTTTG
CATCTAGACAAACTTTGTAGATGGATCAAAATGCTAATATTTTGGTGTTTGGGAAGCTGGCCTC
CAATCTAAACAAACATAGCATGTATTTGTCATGTAGAACTTGTGTTTGATTGGCTGGTAATTAA
AGCATTTCTAATTTTTTGTTGTTTCTTATTTATGCATGCTGTAGTAGGTGATGAAAAAGTTGCA
GAGGTTCAGCGTAAGCGGCACTATGGACCTGATGTATGCTTTAAAGGACAAGCTAGGAAACTCG
CAAAAATGGAAGATGAAAGTGATGATGGAACTGATGAATATTCTCTAC
						
					

			
		

Sequences
Splice junction sequence	AAGCGGCACTATGGACCTGATGTATGCTTTAAAGGACAAGCTAGGAAACTCGCAAAAATGGAAG ATGAAAGTGATGATGGAACTGATGAATATTCTCTACgtgctcttgagaaaggtggtgtgggtgc tcttgaacttgttgctatggacatgaaagccaggttagctcttcctggaacttctcggtggttc ggttctac
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 633 Transcript exons: 7497952-7498012,7498221-7498284,7498485-7498613,7498852- 7498995,7499444-7499546,7499724-7499855 GTGCTCTTGAGAAAGGTGGTGTGGGTGCTCTTGAACTTGTTGCTATGGACATGAAAGCCAGAAC GCTAAGTTATAAGGGTGCTGCTTTTGCTACCGTGGAAGCTCCTCTAGAGGAAAGAATGATGAAT ATGTATAGGAAAGCAGCTGAATTTTGGGCCGAGTTGCGTGTTGAACTCCTTTCAGCAATTGAAT ATTATGCAGAAGACAAGGGCAATTCATCTCAGATTTGGCGGTTATACTGGGCCAGCCATCAGCG TTTCTTTAGGCATATGTGTATGTCGGCTAAAGTACCTGCTGTTGTGAGATTAGTGAAAGAAGCC TTAGCAGAAGAAAAATGTGTGGTGATTGGTCTTCAAAGCACTGGAGAAGCTCGAACAGAGGAAG CTATCAGTAAATATGGTGTTGAAATGGAAGACTTTGTTTCTGGTCCAAGGGAGTTGCTTCTTAA GCTGGTAGATGACAATTATCCTTTGCCTCCGAAACCTGATTGCTTTCAACAAGTAGGTGATGAA AAAGTTGCAGAGGTTCAGCGTAAGCGGCACTATGGACCTGATGTATGCTTTAAAGGACAAGCTA GGAAACTCGCAAAAATGGAAGATGAAAGTGATGATGGAACTGATGAATATTCTCTAC
Genomic sequence	GTGCTCTTGAGAAAGGTGGTGTGGGTGCTCTTGAACTTGTTGCTATGGACATGAAAGCCAGGTT AGCTCTTCCTGGAACTTCTCGGTGGTTCGGTTCTACCTTTTACACACATCGTAGTTGACATGAA GAGCCCTTATGCATTGGGTGTATATACAAATTGCTCTTTCTGTACATAGGTAAGCCAATTACCT CGTTGATACATTTCCTCTTCTAATAATGATTGGTATACCTCTTGTTCAACTTGTCCCAGGGGTA TGTATGTTTGCAGAACGCTAAGTTATAAGGGTGCTGCTTTTGCTACCGTGGAAGCTCCTCTAGA GGAAAGAATGATGGTAAGATTGATGCTCCTTATTTTCTGCTTTTATTTTGGTGGTATGATTAGT TTACTTTGAACCATTTTAAAAATCTTTATAGTTTACAATGTGATTTCACATAACATTAAAAACC AATGTTATGTTTAGCCTTTTCTAATCATTTTTTGTGTAGTGCTGGTGTTGATTTTTATTTCACA TTTTGTTATCTCATGATGCAGAATATGTATAGGAAAGCAGCTGAATTTTGGGCCGAGTTGCGTG TTGAACTCCTTTCAGCAATTGAATATTATGCAGAAGACAAGGGCAATTCATCTCAGATTTGGCG GTTATACTGGGCCAGCCATCAGGTGGTTCTCTTTCATTTTACGTAACAATATGTTTATAACCAT AACACCCTAGCCAATTATGAGATTATGGTTCCCCAGAAATCATATTGCTTCCTTTTGGTATAAT ATATGATCTTTGATTGGGGATTAAATTCTGATTCAGTGGATTTGAACATGGCAATTTGTTTGAC CTTCTGAAAAACTACTACATATGATTAATAACTAAATAACAGTATGCTATGTATATATATTTTT GCAGCGTTTCTTTAGGCATATGTGTATGTCGGCTAAAGTACCTGCTGTTGTGAGATTAGTGAAA GAAGCCTTAGCAGAAGAAAAATGTGTGGTGATTGGTCTTCAAAGCACTGGAGAAGCTCGAACAG AGGAAGCTATCAGTAAATATGTGTGTTTTTCATGCATAGCACTTTCATCCTGTATACCTAAAGA GTGAACTTCACAATACTACAACTTTTTTTTTTTTGTCAAAACCATCTCTTCGCTGCAACTTTAG CCAGACATTTCACAAAACTGCAATCATAGCACAATATTTTGTCAGAAAATCACCACTGTCCCTT GTGTGTATTTAACATGAGTGTCCAACTTGTAGAAGTGAGTCTGACTAGTGGGCCTGACCTATCG GACAGGCCCTTTCTGACGAAGTTGCAGTTATTTTTTGGTTAAATCATCAGTTGACTTTTCTGTT TTCTGAAATGTTCAACAAAAATTGCAGCAAACCGATGGTTTTGATGAGCAAAATAGTTGCAATT CAGTGAAATTTACTCTATTCTAAAACCTATTTTGGTGAATTGATTCTTGGAATTATTTTCCTGT TTACTGAATGAATGTGGTAGGGTGTTGAAATGGAAGACTTTGTTTCTGGTCCAAGGGAGTTGCT TCTTAAGCTGGTAGATGACAATTATCCTTTGCCTCCGAAACCTGATTGCTTTCAACAAGGTTTG CATCTAGACAAACTTTGTAGATGGATCAAAATGCTAATATTTTGGTGTTTGGGAAGCTGGCCTC CAATCTAAACAAACATAGCATGTATTTGTCATGTAGAACTTGTGTTTGATTGGCTGGTAATTAA AGCATTTCTAATTTTTTGTTGTTTCTTATTTATGCATGCTGTAGTAGGTGATGAAAAAGTTGCA GAGGTTCAGCGTAAGCGGCACTATGGACCTGATGTATGCTTTAAAGGACAAGCTAGGAAACTCG CAAAAATGGAAGATGAAAGTGATGATGGAACTGATGAATATTCTCTAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_018355 zma_circ_010208 						
					
						PMCS
						0.333256263

Conservation Information
Conserved circRNAs	osi_circ_018355 zma_circ_010208
PMCS	0.333256263

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							7499731-7498002(+)
7497997-7499852(+)
7498005-7499766(-)
						
						Potential amino acid sequence
						
							MKKLQRFSVSGTMDLMYALKDKLGNSQKWKMKVMMELMNILYVLLRKVVWVLLNLLLWT*(+)
MDMKARTLSYKGAAFATVEAPLEERMMNMYRKAAEFWAELRVELLSAIEYYAEDKGNSSQIWRL
YWASHQRFFRHMCMSAKVPAVVRLVKEALAEEKCVVIGLQSTGEARTEEAISKYGVEMEDFVSG
PRELLLKLVDDNYPLPPKPDCFQQVGDEKVAEVQRKRHYGPDVCFKGQARKLAKMEDESDDGTD
EYSLRALEKGGVGALELVAMDMKARTLSYKGAAFATVEAPLEERMMNMYRKAAEFWAELRVELL
SAIEYYAEDKGNSSQIWRLYWASHQRFFRHMCMSAKVPAVVRLVKEALAEEKCVVIGLQSTGEA
RTEEAISKYGVEMEDFVSGPRELLLKLVDDNYPLPPKPDCFQQVGDEKVAEVQRKRHYGPDVCF
KGQARKLAKMEDESDDGTDEYSLRALEKGGVGALELVAMDMKARTLSYKGAAFATVEAPLEERM
MNMYRKAAEFWAELRVELLSAIEYYAEDKGNSSQIWRLYWASHQRFFRHMCMSAKVPAVVRLVK
EALAEEKCVVIGLQSTGEARTEEAISKYGVEMEDFVSGPRELLLKLVDDNYPLPPKPDCFQQVG
DEKVAEVQRKRHYGPDVCFKGQARKLAKMEDESDDGTDEYSLRALEKGGVGALELVAMDMKART
LSYKGAAFATVEAPLEERMMNMYRKAAEFWAELRVELLSAIEYYAEDKGNSSQIWRLYWASHQR
FFRHMCMSAKVPAVVRLVKEALAEEKCVVIGLQSTGEARTEEAISKYGVEMEDFVSGPRELLLK
LVDDNYPLPPKPDCFQQVGDEKVAEVQRKRHYGPDVCFKGQARKLAKMEDESDDGTDEYSL(+)
MSIATSSRAPTPPFSRARREYSSVPSSLSSSIFASFLACPLKHTSGP*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	7499731-7498002(+) 7497997-7499852(+) 7498005-7499766(-)
Potential amino acid sequence	MKKLQRFSVSGTMDLMYALKDKLGNSQKWKMKVMMELMNILYVLLRKVVWVLLNLLLWT(+) MDMKARTLSYKGAAFATVEAPLEERMMNMYRKAAEFWAELRVELLSAIEYYAEDKGNSSQIWRL YWASHQRFFRHMCMSAKVPAVVRLVKEALAEEKCVVIGLQSTGEARTEEAISKYGVEMEDFVSG PRELLLKLVDDNYPLPPKPDCFQQVGDEKVAEVQRKRHYGPDVCFKGQARKLAKMEDESDDGTD EYSLRALEKGGVGALELVAMDMKARTLSYKGAAFATVEAPLEERMMNMYRKAAEFWAELRVELL SAIEYYAEDKGNSSQIWRLYWASHQRFFRHMCMSAKVPAVVRLVKEALAEEKCVVIGLQSTGEA RTEEAISKYGVEMEDFVSGPRELLLKLVDDNYPLPPKPDCFQQVGDEKVAEVQRKRHYGPDVCF KGQARKLAKMEDESDDGTDEYSLRALEKGGVGALELVAMDMKARTLSYKGAAFATVEAPLEERM MNMYRKAAEFWAELRVELLSAIEYYAEDKGNSSQIWRLYWASHQRFFRHMCMSAKVPAVVRLVK EALAEEKCVVIGLQSTGEARTEEAISKYGVEMEDFVSGPRELLLKLVDDNYPLPPKPDCFQQVG DEKVAEVQRKRHYGPDVCFKGQARKLAKMEDESDDGTDEYSLRALEKGGVGALELVAMDMKART LSYKGAAFATVEAPLEERMMNMYRKAAEFWAELRVELLSAIEYYAEDKGNSSQIWRLYWASHQR FFRHMCMSAKVPAVVRLVKEALAEEKCVVIGLQSTGEARTEEAISKYGVEMEDFVSGPRELLLK LVDDNYPLPPKPDCFQQVGDEKVAEVQRKRHYGPDVCFKGQARKLAKMEDESDDGTDEYSL(+) MSIATSSRAPTPPFSRARREYSSVPSSLSSSIFASFLACPLKHTSGP(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os08g0223766_circ_g.4

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