Plant Circular RNA Database

Detail information of 4_circ_ag.9

			
						 General Information
					
						CircRNA Name
						4_circ_ag.9
					
						ID in PlantcircBase
						ath_circ_032024
					
						Alias
						4:11497463-11501870
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr4: 11497463-11501870  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							ag-circRNA							
									
						Identification method
						find_circ; CIRI2
					
						Parent gene
						
							NA						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						NA
					
						Alternative splicing
						
							4_circ_ag.2 4_circ_ag.3 4_circ_ag.4 4_circ_ag.5 4_circ_ag.6 4_circ_ag.7 4_circ_ag.8 4_circ_ag.10 AT4G21650_circ_g.1 AT4G21650_circ_g.2 AT4G21650_circ_g.3 						
					
						Support reads
						3
					
						Tissues
						root
					
						Exon boundary  
						
							NA-NA							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						NA

General Information
CircRNA Name	4_circ_ag.9
ID in PlantcircBase	ath_circ_032024
Alias	4:11497463-11501870
Organism	Arabidpsis thaliana
Position	chr4: 11497463-11501870 JBrowse»
Reference genome	TAIR10.38
Type	ag-circRNA
Identification method	find_circ; CIRI2
Parent gene	NA
Parent gene annotation	NA
Parent gene strand	NA
Alternative splicing	4_circ_ag.2 4_circ_ag.3 4_circ_ag.4 4_circ_ag.5 4_circ_ag.6 4_circ_ag.7 4_circ_ag.8 4_circ_ag.10 AT4G21650_circ_g.1 AT4G21650_circ_g.2 AT4G21650_circ_g.3
Support reads	3
Tissues	root
Exon boundary	NA-NA "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	NA

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TCAGGGTTTACAAGTCCTCCTCCATAGTCAAATGGATCTGCAAGCTTCTTGTTTGATCCTTCAG
CAAAGATTGGTTCTCCAGATGGACTTGTCCTCCAAGctgttgtgaccaaagctgatctcattgc
agcaggagaccacttagggtgtagagattttaggagaactatgattccagaaacaacaggagtc
gacatcga

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							CTGTTGTGACCAAAGCTGATCTCATTGCAGCAGGAGACCACTTAGGGTGTAGAGATTTTAGGAG
AACTATGATTCCAGAAACAACAGGAGTCGACATCGATGTCCCTGAATGAAGTTTAAATCCGTTG
AACGCACCAGGATCAAGCGGACTCACTGCCGCGAGTATGCTAACACCCGGTGCTGCTATATCAG
GCTACAAAGTAAAAACATGACCAGATATATAAGACCATGTACATAGGAGATTTTTTGTGGGTAT
CATAAGCCTAAAAATATTAAATTAATAGACAAAGCAACTGATTTGGACTTGAGAAGTATCACTG
GAGTGATAATTTCTGATACCAGTCTCTAATGACGTGGCGGCTTGTAATTAGTCGAAAAAACGCG
TTTTTGTGGAAAATGAGAGAAAAAAAAATTATTTGCTCTCGACTATCGCTCCAGTAGCCGATGC
TAGCATGTAATTTCGATTTTACAAATAAATTTTGTTTTTTGATGATACCAATACTGGTCTTACC
ACTGGAAAGAGATTATTATGAGTATCATAAAAAATCTCAAACAATAAACAAGCATTAAAATTAA
TAATAAAAAATTGTTGATGATACTCTATGAGTACCAACTATGTGCATACTCTAACAAGTTTACA
CAAAAAGGATTCAACTTTTAAAGTAAATATAGATAAGAGGCTTTATAACCTTGAGAATGGCTGG
TGAAACCGAGTTAGGCCCTCTAGATGAGAATGCAGCAACCTTAGGCGTTGCAGGCTGGCCGGTT
AGTGTTGTGGCCGCACTAATTCTCACAGTGGGAGATCTGTTGGTTTGACGGATCAATAATGTAT
ATATTCCAGAAATGTATATGAACTTATATAAGTATGTAACAGAGTGTTTTGGCATCAAGATAGA
GAGACCTGGTAGTACGTATGTATTGTAAAATGTGAGTTCCAATCTCATAATCTGTGAAAATGTA
AGAATTGTCTGGGGCAGGACGATCATCAGGCTTTTTGGCTAAGATGACTGCTACTACACCTCTC
CCTGCAATTGAAGAAGGATGAGTACTATCGAATTCAAGAATTGTTTTCCCCTTCACATCAACAT
CATCATCCAAGAAAGCTAAACCCGTTGAAATTTCCGGTCCAGTGAACAAAGATTCAGCCAAATT
AGATACCAACAAAAAACAACAAGTTAATTTGAGCTTTTGGTAGCAAAAACAATAAGGTCGAACT
TTGAACTATTTTCTCAAAGTTATTTTTGGTATCAAACGTACGAAGAAAGTTTGGTTATTCCCAA
GTGTGATCTTTGTAGGAAAAGACCGGTCAAGAGTAGTTGCAGCAACAGTCAAGAGCCATGGAGC
AACATTGCAAATAGTCTGAGCACCAGACCCATCATTTCCCGCCGCAGCTACAACTGTAATCCCT
TTAGCCACCGCATGAAAAGCCGCGATAAAGTCGACCCTCTCGGAATCCTCGGGAATACTCGCCC
CAATAGAAACCGACAAAACATCTACCTGATCATGTATAGCATCATCATAAGCCTTCCACATATC
AGCAGATGAGCATATACCGCCCCATCCCACTACATTCCAACATGCCTTGTACGAGGCTATGCGG
GCCCGAGGGGCTCCACCTCGGACCGTACCTGATAAGAATCCCACATCCGTTAAGAAAAATAAAT
AATGTCTTATAAAACCGGTACCTTGACTCTTAAGTTAACCTTTTTAGTGAATAAAATCCATAAG
GTTTTTGCTATATCACTACCTCGTGCTAGACCATAAAAGCTTGCGTTGGGAACAAATGAGCCAC
CGGCTATTGTGGCTGTATGCGTGCCGTGACCGGTTGCATCTCTGTTGGATTTGAAATCACGGAT
GATTATTCTGTTAAATTTTCCTCCATTCATGGCGAGTAGACCGCTTTGATAGTACTTAGCTCCT
ATAAGCTTTTTGTTGCAATGCATTGTGGCGTTAAACTTTTCTCCTGATCTACATTTTCCTCTCC
AACGCTTAGGTATTGGTCCAAGACCTTGATCGTTGAAGACCTTTGACTCTGGCCATATCCCTGC
ATATACATACATACATGATGTGCGCCAAGGAGAGAGAGCGAGAGAGTTAGATTTAGAGAGAGAG
AGAGAGTTAGATTTAGAGAGAGAGAGAGAGAGAGAGACCTGAATCAACGACGCCGATGATAGCT
TCACTGCCCATGCTGGTGTTATGAAGTAGACCTTTCGCTTTTGCAGATGATGATGAAGAAAAAG
AAGTTGGAATTGGAGAGAGGCCAAGATGATCCCAAATTCTTGTGGTTTTCAGTTTCAGAATCCG
GTTTGGTATAACATGGATTACTTCTGGATGTTCTGTGTTTATATACAAGATTCTGGATTAGGAA
CCAAAAAATCTTCAATGCTCGAAGACCTTACATGTGAGTTTTAGTTTTGTTTGCGTACCTGAGA
TTTTCTTTGCTTGTGAAGATGTAAGAAGCGCCGCGAAACCAGAGAATCCATGCTGGTAGCTGTA
GATCATGGAGTTATGTGCGTCTTCTTTGCTGTATATATTATTAGAGACACAAGTATGAGAAGAG
CTCGTACCAAACAAAGGAAAAGAAACAGAGGAGGGCTCAAGTTCTGTTAAAAACAGAGTATGGA
GAGGTTTTCTTGGGTGACAAGAAACGTATTTGTTATATAATTATGAACTAATCATATGTCTGAA
TATATAATGGAGATTGAATTTAATATATGGAAAGTTTAATGAACCGTCACAGTATATATAAACA
AGTTCAATAGGTTAATGTCCAATATAGAGATTAGTCTCTATAGAAGAAATTTTGGCCTTCTTTT
AAGGATGATTAAAGTAAAGTACCTTTGAAGAAGTGATTCAAGCATTTGATGATGAGAAGCGGTG
AGAAGTTCAGGATCATCGTGTTCCCTTTGGCCAAGATACACTATATAAACCTTATAAATCCGCC
AAAAAACCACCGAAAAAGTTAGAAATATCAGTAAATTCTTAGCGATTTTTCGCAAAATAGAACA
CCGTTTTAATTTAGTTACATTTACATACCTTGCTGTTACTATCTGAGGCTCCTTCAGCTGTAAG
AAAACTCAGCTCTGTGTTCAGAAATAGGACCAAAGCAATTGCCAATAGAAACACAAGTTTGGAA
CTTAGGAAAGAGTTCTCCATTTTATGCAGTGAGAAGTGTGTGTGATTCTGTCGAAGAGAAGCTT
TATTATATACATAGTGTGTCTTTAGGGAGTGAACTATTGCGTAAGATTCGAATTCAATTATTGG
TTTATTCGGCTCAATATCGGTATGGTAATTCTGAATCATACCGGTTTTATCTCTTGTGTAACGT
TATTTCCCGCCATGGATCTCAAGAGGGCTCAGTTTTAACTCTTACAATTGTCGTCTTTCTTATT
ATTGATGTCTTTCTTATTATAGATATACTAATAGTGTACCTACTTTAATTTCAGCTGAGTATAA
GAGGATATCAATCATTTCTAACTAATAGAGTAGATTAGGTATCTGTATAAGTTGGTAGATCATG
ATTGTCTGAATTACTCATTTTAGTAAAACAGATTATATTCTGAACTTTAATATATATAGGATCA
CAGGAGAGCAAACCAGTTCCATTTTTTGCATTTATGTGTACACTAAAATATCACATCTTTATAA
TATATAGTCAATATGATGATTATATATTTGTATGTAATGTTACAGCTATAGGTTATGATCATGA
TCTTTTATAAGAGATTCTAAGGTATTTGTGATTTGATCTTTTATTATGATGATCTTGAATCTTG
ATTTGACATTTTAGACTTAGATTTGCAACAAATTACATAAGAACAAACACAATGAGCATCATAT
GGCTCTTTTTATTACATCGAAATCGTTGTCTTAACAGAGACAGGGATTATAACATCATGAACAC
CATCACTCCATGTTAAGCTTCCAAAGAAGTAGCCAGTGTTGACTTTGTGACTTGTTTTAGCCTT
AACCGAGAAAGTGAGTACTCTCTTAGCTGCAGACTTAAACACGAGTGTGGTTGGGTTCACGGTG
AGAGTTATACCGAGAGGAGACTCGATCACAGCTCTATAGACTGACTTGATGGGTCCAACATTGG
TTACAGTTCTTGTGAGTGTGACTTCTTTCTCAAGATTTGGAATTGTGATTGAAGGTAAGTTGAT
ATCAAGCATTGATGGCTTAGGAATTGGGCAATTAGTCTTTTTACCGAGCACACGAGAGATTGAA
GAGTCGTTGTAACCCGCAGAACACATATACTTGATGTAATCCACAATCCCCATATCGTAGACAA
GTCCTGGTTTAGCAGCTTTTTCAGGGTTTACAAGTCCTCCTCCATAGTCAAATGGATCTGCAAG
CTTCTTGTTTGATCCTTCAGCAAAGATTGGTTCTCCAGATGGACTTGTCCTCCAAG
						
					

			
		

Sequences
Splice junction sequence	TCAGGGTTTACAAGTCCTCCTCCATAGTCAAATGGATCTGCAAGCTTCTTGTTTGATCCTTCAG CAAAGATTGGTTCTCCAGATGGACTTGTCCTCCAAGctgttgtgaccaaagctgatctcattgc agcaggagaccacttagggtgtagagattttaggagaactatgattccagaaacaacaggagtc gacatcga
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CTGTTGTGACCAAAGCTGATCTCATTGCAGCAGGAGACCACTTAGGGTGTAGAGATTTTAGGAG AACTATGATTCCAGAAACAACAGGAGTCGACATCGATGTCCCTGAATGAAGTTTAAATCCGTTG AACGCACCAGGATCAAGCGGACTCACTGCCGCGAGTATGCTAACACCCGGTGCTGCTATATCAG GCTACAAAGTAAAAACATGACCAGATATATAAGACCATGTACATAGGAGATTTTTTGTGGGTAT CATAAGCCTAAAAATATTAAATTAATAGACAAAGCAACTGATTTGGACTTGAGAAGTATCACTG GAGTGATAATTTCTGATACCAGTCTCTAATGACGTGGCGGCTTGTAATTAGTCGAAAAAACGCG TTTTTGTGGAAAATGAGAGAAAAAAAAATTATTTGCTCTCGACTATCGCTCCAGTAGCCGATGC TAGCATGTAATTTCGATTTTACAAATAAATTTTGTTTTTTGATGATACCAATACTGGTCTTACC ACTGGAAAGAGATTATTATGAGTATCATAAAAAATCTCAAACAATAAACAAGCATTAAAATTAA TAATAAAAAATTGTTGATGATACTCTATGAGTACCAACTATGTGCATACTCTAACAAGTTTACA CAAAAAGGATTCAACTTTTAAAGTAAATATAGATAAGAGGCTTTATAACCTTGAGAATGGCTGG TGAAACCGAGTTAGGCCCTCTAGATGAGAATGCAGCAACCTTAGGCGTTGCAGGCTGGCCGGTT AGTGTTGTGGCCGCACTAATTCTCACAGTGGGAGATCTGTTGGTTTGACGGATCAATAATGTAT ATATTCCAGAAATGTATATGAACTTATATAAGTATGTAACAGAGTGTTTTGGCATCAAGATAGA GAGACCTGGTAGTACGTATGTATTGTAAAATGTGAGTTCCAATCTCATAATCTGTGAAAATGTA AGAATTGTCTGGGGCAGGACGATCATCAGGCTTTTTGGCTAAGATGACTGCTACTACACCTCTC CCTGCAATTGAAGAAGGATGAGTACTATCGAATTCAAGAATTGTTTTCCCCTTCACATCAACAT CATCATCCAAGAAAGCTAAACCCGTTGAAATTTCCGGTCCAGTGAACAAAGATTCAGCCAAATT AGATACCAACAAAAAACAACAAGTTAATTTGAGCTTTTGGTAGCAAAAACAATAAGGTCGAACT TTGAACTATTTTCTCAAAGTTATTTTTGGTATCAAACGTACGAAGAAAGTTTGGTTATTCCCAA GTGTGATCTTTGTAGGAAAAGACCGGTCAAGAGTAGTTGCAGCAACAGTCAAGAGCCATGGAGC AACATTGCAAATAGTCTGAGCACCAGACCCATCATTTCCCGCCGCAGCTACAACTGTAATCCCT TTAGCCACCGCATGAAAAGCCGCGATAAAGTCGACCCTCTCGGAATCCTCGGGAATACTCGCCC CAATAGAAACCGACAAAACATCTACCTGATCATGTATAGCATCATCATAAGCCTTCCACATATC AGCAGATGAGCATATACCGCCCCATCCCACTACATTCCAACATGCCTTGTACGAGGCTATGCGG GCCCGAGGGGCTCCACCTCGGACCGTACCTGATAAGAATCCCACATCCGTTAAGAAAAATAAAT AATGTCTTATAAAACCGGTACCTTGACTCTTAAGTTAACCTTTTTAGTGAATAAAATCCATAAG GTTTTTGCTATATCACTACCTCGTGCTAGACCATAAAAGCTTGCGTTGGGAACAAATGAGCCAC CGGCTATTGTGGCTGTATGCGTGCCGTGACCGGTTGCATCTCTGTTGGATTTGAAATCACGGAT GATTATTCTGTTAAATTTTCCTCCATTCATGGCGAGTAGACCGCTTTGATAGTACTTAGCTCCT ATAAGCTTTTTGTTGCAATGCATTGTGGCGTTAAACTTTTCTCCTGATCTACATTTTCCTCTCC AACGCTTAGGTATTGGTCCAAGACCTTGATCGTTGAAGACCTTTGACTCTGGCCATATCCCTGC ATATACATACATACATGATGTGCGCCAAGGAGAGAGAGCGAGAGAGTTAGATTTAGAGAGAGAG AGAGAGTTAGATTTAGAGAGAGAGAGAGAGAGAGAGACCTGAATCAACGACGCCGATGATAGCT TCACTGCCCATGCTGGTGTTATGAAGTAGACCTTTCGCTTTTGCAGATGATGATGAAGAAAAAG AAGTTGGAATTGGAGAGAGGCCAAGATGATCCCAAATTCTTGTGGTTTTCAGTTTCAGAATCCG GTTTGGTATAACATGGATTACTTCTGGATGTTCTGTGTTTATATACAAGATTCTGGATTAGGAA CCAAAAAATCTTCAATGCTCGAAGACCTTACATGTGAGTTTTAGTTTTGTTTGCGTACCTGAGA TTTTCTTTGCTTGTGAAGATGTAAGAAGCGCCGCGAAACCAGAGAATCCATGCTGGTAGCTGTA GATCATGGAGTTATGTGCGTCTTCTTTGCTGTATATATTATTAGAGACACAAGTATGAGAAGAG CTCGTACCAAACAAAGGAAAAGAAACAGAGGAGGGCTCAAGTTCTGTTAAAAACAGAGTATGGA GAGGTTTTCTTGGGTGACAAGAAACGTATTTGTTATATAATTATGAACTAATCATATGTCTGAA TATATAATGGAGATTGAATTTAATATATGGAAAGTTTAATGAACCGTCACAGTATATATAAACA AGTTCAATAGGTTAATGTCCAATATAGAGATTAGTCTCTATAGAAGAAATTTTGGCCTTCTTTT AAGGATGATTAAAGTAAAGTACCTTTGAAGAAGTGATTCAAGCATTTGATGATGAGAAGCGGTG AGAAGTTCAGGATCATCGTGTTCCCTTTGGCCAAGATACACTATATAAACCTTATAAATCCGCC AAAAAACCACCGAAAAAGTTAGAAATATCAGTAAATTCTTAGCGATTTTTCGCAAAATAGAACA CCGTTTTAATTTAGTTACATTTACATACCTTGCTGTTACTATCTGAGGCTCCTTCAGCTGTAAG AAAACTCAGCTCTGTGTTCAGAAATAGGACCAAAGCAATTGCCAATAGAAACACAAGTTTGGAA CTTAGGAAAGAGTTCTCCATTTTATGCAGTGAGAAGTGTGTGTGATTCTGTCGAAGAGAAGCTT TATTATATACATAGTGTGTCTTTAGGGAGTGAACTATTGCGTAAGATTCGAATTCAATTATTGG TTTATTCGGCTCAATATCGGTATGGTAATTCTGAATCATACCGGTTTTATCTCTTGTGTAACGT TATTTCCCGCCATGGATCTCAAGAGGGCTCAGTTTTAACTCTTACAATTGTCGTCTTTCTTATT ATTGATGTCTTTCTTATTATAGATATACTAATAGTGTACCTACTTTAATTTCAGCTGAGTATAA GAGGATATCAATCATTTCTAACTAATAGAGTAGATTAGGTATCTGTATAAGTTGGTAGATCATG ATTGTCTGAATTACTCATTTTAGTAAAACAGATTATATTCTGAACTTTAATATATATAGGATCA CAGGAGAGCAAACCAGTTCCATTTTTTGCATTTATGTGTACACTAAAATATCACATCTTTATAA TATATAGTCAATATGATGATTATATATTTGTATGTAATGTTACAGCTATAGGTTATGATCATGA TCTTTTATAAGAGATTCTAAGGTATTTGTGATTTGATCTTTTATTATGATGATCTTGAATCTTG ATTTGACATTTTAGACTTAGATTTGCAACAAATTACATAAGAACAAACACAATGAGCATCATAT GGCTCTTTTTATTACATCGAAATCGTTGTCTTAACAGAGACAGGGATTATAACATCATGAACAC CATCACTCCATGTTAAGCTTCCAAAGAAGTAGCCAGTGTTGACTTTGTGACTTGTTTTAGCCTT AACCGAGAAAGTGAGTACTCTCTTAGCTGCAGACTTAAACACGAGTGTGGTTGGGTTCACGGTG AGAGTTATACCGAGAGGAGACTCGATCACAGCTCTATAGACTGACTTGATGGGTCCAACATTGG TTACAGTTCTTGTGAGTGTGACTTCTTTCTCAAGATTTGGAATTGTGATTGAAGGTAAGTTGAT ATCAAGCATTGATGGCTTAGGAATTGGGCAATTAGTCTTTTTACCGAGCACACGAGAGATTGAA GAGTCGTTGTAACCCGCAGAACACATATACTTGATGTAATCCACAATCCCCATATCGTAGACAA GTCCTGGTTTAGCAGCTTTTTCAGGGTTTACAAGTCCTCCTCCATAGTCAAATGGATCTGCAAG CTTCTTGTTTGATCCTTCAGCAAAGATTGGTTCTCCAGATGGACTTGTCCTCCAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.569925939

Conservation Information
Conserved circRNAs	NA
PMCS	0.569925939

			
						 Functional Information
					
						Coding potential
						NA
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	NA
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Philips et al., 2020

circRNA

Detail information of 4_circ_ag.9

Address

Any questions?

Supported by