Plant Circular RNA Database

Detail information of Zm00001d023689_circ_g.5

			
						 General Information
					
						CircRNA Name
						Zm00001d023689_circ_g.5
					
						ID in PlantcircBase
						zma_circ_010169
					
						Alias
						zma_circ_0000115, GRMZM5G862959_C2
					
						Organism
						
							Zea mays						
					
						Position
						
							chr10: 15556755-15565068  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						find_circ, CIRI2
					
						Parent gene
						
							Zm00001d023689						
					
						Parent gene annotation
						
							SGF29 tudor-like domain
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Zm00001d023689_circ_g.2 Zm00001d023689_circ_g.3 Zm00001d023689_circ_g.4 						
					
						Support reads
						NA
					
						Tissues
						leaf, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d023689_T004:3
Zm00001d023689_T006:3
Zm00001d023689_T001:3
Zm00001d023689_T003:2
Zm00001d023689_T002:3

General Information
CircRNA Name	Zm00001d023689_circ_g.5
ID in PlantcircBase	zma_circ_010169
Alias	zma_circ_0000115, GRMZM5G862959_C2
Organism	Zea mays
Position	chr10: 15556755-15565068 JBrowse»
Reference genome	AGPv4.38
Type	u-circRNA
Identification method	find_circ, CIRI2
Parent gene	Zm00001d023689
Parent gene annotation	SGF29 tudor-like domain
Parent gene strand	+
Alternative splicing	Zm00001d023689_circ_g.2 Zm00001d023689_circ_g.3 Zm00001d023689_circ_g.4
Support reads	NA
Tissues	leaf, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d023689_T004:3 Zm00001d023689_T006:3 Zm00001d023689_T001:3 Zm00001d023689_T003:2 Zm00001d023689_T002:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TCCTTCGAGTGCCCCGGATTTTGGGCAGGGTCGACAAGTTTTAGCTGTTTATCCTGGCACAACA
GCATTATACAGAGCTACCGTAGCTTCTCATCGTAAGgttgctgctagggtaaaatcagatgacg
aaaaagatgagtggtttgtcgtgaaggtcatacatttcgataaggaaacaaaagagtatgtgtc
tactttat

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 285
Transcript exons: 15556755-15556837,15557441-15557491,15564918-15565068
GTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTGAAGGTCATACATT
TCGATAAGGAAACAAAAGAGTATGAAGTACTTGATGAAGAACCAGGTGATGACGAAGAGAGCAC
TCAAAAGAAGTACAAGTTGCCTATGTCTTACATTATTCCATTTCCGAAGAAAGGGGATCCTTCG
AGTGCCCCGGATTTTGGGCAGGGTCGACAAGTTTTAGCTGTTTATCCTGGCACAACAGCATTAT
ACAGAGCTACCGTAGCTTCTCATCGTAAG
						
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	TCCTTCGAGTGCCCCGGATTTTGGGCAGGGTCGACAAGTTTTAGCTGTTTATCCTGGCACAACA GCATTATACAGAGCTACCGTAGCTTCTCATCGTAAGgttgctgctagggtaaaatcagatgacg aaaaagatgagtggtttgtcgtgaaggtcatacatttcgataaggaaacaaaagagtatgtgtc tactttat
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 285 Transcript exons: 15556755-15556837,15557441-15557491,15564918-15565068 GTTGCTGCTAGGGTAAAATCAGATGACGAAAAAGATGAGTGGTTTGTCGTGAAGGTCATACATT TCGATAAGGAAACAAAAGAGTATGAAGTACTTGATGAAGAACCAGGTGATGACGAAGAGAGCAC TCAAAAGAAGTACAAGTTGCCTATGTCTTACATTATTCCATTTCCGAAGAAAGGGGATCCTTCG AGTGCCCCGGATTTTGGGCAGGGTCGACAAGTTTTAGCTGTTTATCCTGGCACAACAGCATTAT ACAGAGCTACCGTAGCTTCTCATCGTAAG
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							osa_circ_011168 osi_circ_003397 osi_circ_010424 						
					
						PMCS
						0.123955902

Conservation Information
Conserved circRNAs	osa_circ_011168 osi_circ_003397 osi_circ_010424
PMCS	0.123955902

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							15564935-15565065(+)
15556815-15565061(-)
						
						Potential amino acid sequence
						
							MSYIIPFPKKGDPSSAPDFGQGRQVLAVYPGTTALYRATVASHRKVAARVKSDDEKDEWFVVKV
IHFDKETKEYEVLDEEPGDDEESTQKKYKLPMSYIIPFPKKGDPSSAPDFGQGRQVLAVYPGTT
ALYRATVASHRKVAARVKSDDEKDEWFVVKVIHFDKETKEYEVLDEEPGDDEESTQKKYKLPMS
YIIPFPKKGDPSSAPDFGQGRQVLAVYPGTTALYRATVASHRKVAARVKSDDEKDEWFVVKVIH
FDKETKEYEVLDEEPGDDEESTQKKYKLPMSYIIPFPKKGDPSSAPDFGQGRQVLAVYPGTTAL
YRATVASHRK(+)
MTFTTNHSSFSSSDFTLAATLR*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to drought stress

Functional Information
Coding potential	Y
Potential coding position	15564935-15565065(+) 15556815-15565061(-)
Potential amino acid sequence	MSYIIPFPKKGDPSSAPDFGQGRQVLAVYPGTTALYRATVASHRKVAARVKSDDEKDEWFVVKV IHFDKETKEYEVLDEEPGDDEESTQKKYKLPMSYIIPFPKKGDPSSAPDFGQGRQVLAVYPGTT ALYRATVASHRKVAARVKSDDEKDEWFVVKVIHFDKETKEYEVLDEEPGDDEESTQKKYKLPMS YIIPFPKKGDPSSAPDFGQGRQVLAVYPGTTALYRATVASHRKVAARVKSDDEKDEWFVVKVIH FDKETKEYEVLDEEPGDDEESTQKKYKLPMSYIIPFPKKGDPSSAPDFGQGRQVLAVYPGTTAL YRATVASHRK(+) MTFTTNHSSFSSSDFTLAATLR*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to drought stress

			
						 Other Information
					
						References
						Ma et al., 2021b;Zhang et al., 2019

circRNA

Detail information of Zm00001d023689_circ_g.5

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