Plant Circular RNA Database

Detail information of AT2G28540_circ_g.2

			
						 General Information
					
						CircRNA Name
						AT2G28540_circ_g.2
					
						ID in PlantcircBase
						ath_circ_015394
					
						Alias
						NA
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr2: 12218646-12219813  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						KNIFE, PcircRNA_finder, circRNA_finder, find_circ
					
						Parent gene
						
							AT2G28540						
					
						Parent gene annotation
						
							Putative uncharacterized protein At2g28540
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT2G28540_circ_g.3 AT2G28540_circ_g.4 AT2G28540_circ_g.5 AT2G28540_circ_g.6 AT2G28540_circ_g.7 AT2G28540_circ_g.8 AT2G28540_circ_g.9 AT2G28540_circ_g.10 						
					
						Support reads
						3
					
						Tissues
						whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT2G28540.1:4
AT2G28540.2:4

General Information
CircRNA Name	AT2G28540_circ_g.2
ID in PlantcircBase	ath_circ_015394
Alias	NA
Organism	Arabidpsis thaliana
Position	chr2: 12218646-12219813 JBrowse»
Reference genome	TAIR10.38
Type	ue-circRNA
Identification method	KNIFE, PcircRNA_finder, circRNA_finder, find_circ
Parent gene	AT2G28540
Parent gene annotation	Putative uncharacterized protein At2g28540
Parent gene strand	+
Alternative splicing	AT2G28540_circ_g.3 AT2G28540_circ_g.4 AT2G28540_circ_g.5 AT2G28540_circ_g.6 AT2G28540_circ_g.7 AT2G28540_circ_g.8 AT2G28540_circ_g.9 AT2G28540_circ_g.10
Support reads	3
Tissues	whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT2G28540.1:4 AT2G28540.2:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GAAGAGAATACTTTGGTCAATATGGGAAAGTCGTCAAAGTAGCAATGTCTCGGACTGCAGCTGG
GGCTGTCCAACAATTTCCAAACAATACTTGCAGTGTgtgacgatgaatgagaaaggagaaaaga
cttgtcctctatgtactgaggagatggacttgactgatcaacatcttaaaccttgcaaatgtgg
ttatcagg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 488
Transcript exons: 12218646-12218744,12219240-12219367,12219454-12219604,12
219704-12219813
GTGACGATGAATGAGAAAGGAGAAAAGACTTGTCCTCTATGTACTGAGGAGATGGACTTGACTG
ATCAACATCTTAAACCTTGCAAATGTGGTTATCAGATATGTGTTTGGTGTTGGCATCATATTAT
AGAGATGGCTGAGAAAGATAAGACAGAGGGTCGTTGTCCTGCATGTCGTACCCGTTATGATAAG
GAAAAGATTGTAGGGATGACAGTCAGCTGTGAAAGGTTGGTTGCTGAATTTTACATTGACCGGA
AAAAGTCACAGAAGGCGAAGCCTAAACCTGCGGAGGGAAGGAAAGATCTAACTGGTGTGAGAGT
TATTCAGAGAAACTTGGTTTACGTTATGAGCTTGCCTTTTGACCTGGCAGATGAAGATATGTTT
CAGCGAAGAGAATACTTTGGTCAATATGGGAAAGTCGTCAAAGTAGCAATGTCTCGGACTGCAG
CTGGGGCTGTCCAACAATTTCCAAACAATACTTGCAGTGT
						
					

						Genomic sequence
						
							GTGACGATGAATGAGAAAGGAGAAAAGACTTGTCCTCTATGTACTGAGGAGATGGACTTGACTG
ATCAACATCTTAAACCTTGCAAATGTGGTTATCAGGTTTGATACATTACAACCACTTTTCCTCA
CAATGTGGCTCTCTTGTTTGTTTATTGTCACTGTTTGAGTAGAATGTGTGTTTTGGGGTCTTGT
TTATAGTCTGAAACCGTTGGGTGTTGATACGTTTCATTAAGGGTATTGCAATTTGCTATGGGCA
GAGTTTTTTCTCACCCTATTCTTTTGAGTTTAAGCACCAAAGAATCTAAGCTTCTGTTAATTCT
GTATGCTGTATATATTAACACATGCCTCTGTGGAGAGTTGTCTTCTGCTTGAAAAGGACCACGA
TGTGTAGTCTCAGATTTCGAATTGTTTTATAGGTTGTCCCACATTGTCTATAAGCATCTTGTGA
ATGGACCTTTTCTTATAATCGATGTTTAAAGCTGACATTACCCAGCCTTATATTGTTGCCTGTT
CATTTATGTTTGAATTATTCAGTGTGCCACTGTGGTGATCTATAAGAAACCCTTTTTATGTAAA
TACATGTACTTTGTGCAGATATGTGTTTGGTGTTGGCATCATATTATAGAGATGGCTGAGAAAG
ATAAGACAGAGGGTCGTTGTCCTGCATGTCGTACCCGTTATGATAAGGAAAAGATTGTAGGGAT
GACAGTCAGCTGTGAAAGGTAGATCTGAAGCTGAAAACAAACCCTTTTTTGTTTCTGTGAAACG
TGTAAAAACTTATCTTTTTCTTGTTCTGATAATTCACTAGGTTGGTTGCTGAATTTTACATTGA
CCGGAAAAAGTCACAGAAGGCGAAGCCTAAACCTGCGGAGGGAAGGAAAGATCTAACTGGTGTG
AGAGTTATTCAGAGAAACTTGGTTTACGTTATGAGCTTGCCTTTTGACCTGGCAGATGAAGATG
TAACTTAAACAGCCTTAACTTATCTCCTTGGCACTCACATTTGCTTTGAAAAGAGATAACCTTT
TTCATATCATTAATCTGGGTTGTTATGTATGCAGATGTTTCAGCGAAGAGAATACTTTGGTCAA
TATGGGAAAGTCGTCAAAGTAGCAATGTCTCGGACTGCAGCTGGGGCTGTCCAACAATTTCCAA
ACAATACTTGCAGTGT
						
					

			
		

Sequences
Splice junction sequence	GAAGAGAATACTTTGGTCAATATGGGAAAGTCGTCAAAGTAGCAATGTCTCGGACTGCAGCTGG GGCTGTCCAACAATTTCCAAACAATACTTGCAGTGTgtgacgatgaatgagaaaggagaaaaga cttgtcctctatgtactgaggagatggacttgactgatcaacatcttaaaccttgcaaatgtgg ttatcagg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 488 Transcript exons: 12218646-12218744,12219240-12219367,12219454-12219604,12 219704-12219813 GTGACGATGAATGAGAAAGGAGAAAAGACTTGTCCTCTATGTACTGAGGAGATGGACTTGACTG ATCAACATCTTAAACCTTGCAAATGTGGTTATCAGATATGTGTTTGGTGTTGGCATCATATTAT AGAGATGGCTGAGAAAGATAAGACAGAGGGTCGTTGTCCTGCATGTCGTACCCGTTATGATAAG GAAAAGATTGTAGGGATGACAGTCAGCTGTGAAAGGTTGGTTGCTGAATTTTACATTGACCGGA AAAAGTCACAGAAGGCGAAGCCTAAACCTGCGGAGGGAAGGAAAGATCTAACTGGTGTGAGAGT TATTCAGAGAAACTTGGTTTACGTTATGAGCTTGCCTTTTGACCTGGCAGATGAAGATATGTTT CAGCGAAGAGAATACTTTGGTCAATATGGGAAAGTCGTCAAAGTAGCAATGTCTCGGACTGCAG CTGGGGCTGTCCAACAATTTCCAAACAATACTTGCAGTGT
Genomic sequence	GTGACGATGAATGAGAAAGGAGAAAAGACTTGTCCTCTATGTACTGAGGAGATGGACTTGACTG ATCAACATCTTAAACCTTGCAAATGTGGTTATCAGGTTTGATACATTACAACCACTTTTCCTCA CAATGTGGCTCTCTTGTTTGTTTATTGTCACTGTTTGAGTAGAATGTGTGTTTTGGGGTCTTGT TTATAGTCTGAAACCGTTGGGTGTTGATACGTTTCATTAAGGGTATTGCAATTTGCTATGGGCA GAGTTTTTTCTCACCCTATTCTTTTGAGTTTAAGCACCAAAGAATCTAAGCTTCTGTTAATTCT GTATGCTGTATATATTAACACATGCCTCTGTGGAGAGTTGTCTTCTGCTTGAAAAGGACCACGA TGTGTAGTCTCAGATTTCGAATTGTTTTATAGGTTGTCCCACATTGTCTATAAGCATCTTGTGA ATGGACCTTTTCTTATAATCGATGTTTAAAGCTGACATTACCCAGCCTTATATTGTTGCCTGTT CATTTATGTTTGAATTATTCAGTGTGCCACTGTGGTGATCTATAAGAAACCCTTTTTATGTAAA TACATGTACTTTGTGCAGATATGTGTTTGGTGTTGGCATCATATTATAGAGATGGCTGAGAAAG ATAAGACAGAGGGTCGTTGTCCTGCATGTCGTACCCGTTATGATAAGGAAAAGATTGTAGGGAT GACAGTCAGCTGTGAAAGGTAGATCTGAAGCTGAAAACAAACCCTTTTTTGTTTCTGTGAAACG TGTAAAAACTTATCTTTTTCTTGTTCTGATAATTCACTAGGTTGGTTGCTGAATTTTACATTGA CCGGAAAAAGTCACAGAAGGCGAAGCCTAAACCTGCGGAGGGAAGGAAAGATCTAACTGGTGTG AGAGTTATTCAGAGAAACTTGGTTTACGTTATGAGCTTGCCTTTTGACCTGGCAGATGAAGATG TAACTTAAACAGCCTTAACTTATCTCCTTGGCACTCACATTTGCTTTGAAAAGAGATAACCTTT TTCATATCATTAATCTGGGTTGTTATGTATGCAGATGTTTCAGCGAAGAGAATACTTTGGTCAA TATGGGAAAGTCGTCAAAGTAGCAATGTCTCGGACTGCAGCTGGGGCTGTCCAACAATTTCCAA ACAATACTTGCAGTGT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.212906278

Conservation Information
Conserved circRNAs	NA
PMCS	0.212906278

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of AT2G28540_circ_g.2

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