Plant Circular RNA Database

Detail information of GLYMA_19G250600_circ_g.7

			
						 General Information
					
						CircRNA Name
						GLYMA_19G250600_circ_g.7
					
						ID in PlantcircBase
						gma_circ_005085
					
						Alias
						Gm19circRNA2443
					
						Organism
						
							Glycine max						
					
						Position
						
							chr19: 49659317-49661626  JBrowse»						
					
						Reference genome
						
							v2.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						Tophat, CIRI
					
						Parent gene
						
							GLYMA_19G250600						
					
						Parent gene annotation
						
							hypothetical protein
							
						Parent gene strand
						+
					
						Alternative splicing
						
							GLYMA_19G250600_circ_g.2 GLYMA_19G250600_circ_g.3 GLYMA_19G250600_circ_g.4 GLYMA_19G250600_circ_g.5 GLYMA_19G250600_circ_g.6 						
					
						Support reads
						NA
					
						Tissues
						leaf, root, stem
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						KRG97079:5

General Information
CircRNA Name	GLYMA_19G250600_circ_g.7
ID in PlantcircBase	gma_circ_005085
Alias	Gm19circRNA2443
Organism	Glycine max
Position	chr19: 49659317-49661626 JBrowse»
Reference genome	v2.0.38
Type	e-circRNA
Identification method	Tophat, CIRI
Parent gene	GLYMA_19G250600
Parent gene annotation	hypothetical protein
Parent gene strand	+
Alternative splicing	GLYMA_19G250600_circ_g.2 GLYMA_19G250600_circ_g.3 GLYMA_19G250600_circ_g.4 GLYMA_19G250600_circ_g.5 GLYMA_19G250600_circ_g.6
Support reads	NA
Tissues	leaf, root, stem
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	KRG97079:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CTGCAATACTACTCAACGTTTTATCAGATCATCAAGAACGGTTCCTGTTCAAAAGCCATCTGTA
CCCTCTTCTAAGCCTAGTTTCTATTGTGGTACTCAAgtggatcattgttttgatatatttgtta
caaaattatgtgaaaccattttcacatattacaaaagttgggcggcatggtatgctcattattt
ctctggct

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							GTGGATCATTGTTTTGATATATTTGTTACAAAATTATGTGAAACCATTTTCACATATTACAAAA
GTTGGGCGGCATGGTATGCTCATTATTTCTCTGGCTTATTCAGCTTTTCTTTTGATTATTTCCA
TGGACAAACCTTTTTTCTTACTGATGGAGGCCTAAATCATGCAGTGAATTGCTTGATCCTTCAT
TCCTCTTTGCTTCGGACAATGCAGAAAAGTATGCCGTGCAACCCATAAGGCTCAATATGCTGCT
TAAAATGACTAGAGTGAAGGTGAATGAATTACACTATCATAATTTTTATTTTCCATGATTAGAT
TTGGTGCATTAAAGTGTAAACTGGGTGTATGCTACCAGATTGATAATTTTTTGATATGAAAATA
GCCCAAGGTGCAGGGATTGAAAATATATGTACATGCTATTAGATACCCATCTCCCAGCAGGGTT
TGGAAATGCTTGACTGAAGCCTTAGTTTAAGTGAACAAAGCTTAACTGTTTCATTACTTCTTTA
TTTAGTATTTATCAGGGTGTTAACAATTCAAATCTCATTATCTTTTCAAGTGATCTTGGAATCA
TGTGACTTACTATAACTTCAGGAATTATACAAAACAGATGTCTTTGTGTACTATTATAAGCAGG
AATAAGTTTTTAGAATATTTTTCTTCCTAAGAATTTAATATTTTACTGGTTATCAGTTAGTTGC
ATTTGCATCAAAATCTTCCCACAAGTTAGCAAAGCTTGTTGATCAGAAAGTCCAAGTTTGAAAT
CTTGTGAATGGAAAAACAGGTGCTGGGAGAGTGTTGCCTGTCTAATTGGTTCAACCCAACTCAA
CTTGGATTAGTTAAGGTCCAAGGTGGCTTTTGGATGTTTGGTCTATCTTTTTGGAGCTTCTCCT
CTCTTTGAAAGGAGAAACCAGGCCAAATACAATTCTAAAAAAACTATTAAGTATAAAAGTGGCT
TTTTTCTATATGAACTAAGGAAGAAAACATAAAAAAAGAAGAGCTTTTAGATAATCTATATTTT
AGGAACTTCTGCTTTCCCTTCTTCACTTTTCCAAATCCCAAAACTCAGTAGTGTGCTAACCAAG
AATTATTAACTAAAAATTTACTTTTAAGCTGAACGAATATAAAATTATCAAACACATTCAATTA
TAAAGTTTTATTTTTACTTGAACTTTAAAGTAGTTTTTAAGATCCAAAAAAATCAATCCAAAGG
CCACCTAGTGAAAAAGTTCTCTAAAGTTATTGACGGAGAGAATAAAGACATCTTGGTAGGTGTT
TTACTTTTCAATATTTTGTTTCTAGGGTAATTCCATTTTAATCATTAATAAAATTCTTGTAGAA
ACTTCTGGTGTCAAAAGGATGGAAAGTAGGTTCCGATCATCTTCAATGCCACATCCTGCAACAA
ACTAATGGAGATTGTTTAAGAAAATAATAATGAAGGCTAAGATTGATGCTTACATGAATTATAA
CTTTTATTTTCATGGTGTATTCGTTTTTCTATTTAGATGATTTCTCTCACACTCACACTCCCAC
ACTCACATGTATGTTCTGCTGGATGTCATAATTAGAATTTGAGTGAGTTGCAATTACTCTCATG
TGATTAAATATCTTTTGTTGCACAGCTGCTTGGAAGGATGATTAATTTGCGAAGTTTGATCACT
GAACGGATGAACAAGGTTTTCCGTGAAAATATTGAGTTTTTATTTGATCGTTTTGAGTGTCAGG
ATCTGTGTGCCATAGTGGTTAGTGATTTTACCTTTGCTACTTCCCTGCCCAACAATTATGAACT
ACTTTTTCTTTTCTCAATGTTCATTAACTAATGTAATACTGACTATTTTATTTCAGGAATTAGA
GAAGTTGTTGGATGTCTTAAAACATTCCCATGAACTACTGTCTAGAGATCTTTCTGTAGACTCT
TTTAGTCTCATGTTGAATGAGATGCAAGAGAACATTTCCCTTGTATCATTTTCTAGTCGATTGG
CCTCACAGGTTTGCCCCTTTGAGCTCTAAAGATGTACTTTAATTTTGATGTCTTCCTGTTATTT
TTTTAATCTTGAATCCTCATATTGAAAGTTTGAAACCACTATTTGAATGGTAATTAATAATTTC
ATGACTTGATCTGTTTCCTACAATTTCATTGATATATTTCTTTACGTATGCTAGATTTGGTCTG
AGATGCATAGTGATTTTTTGCCAAATTTCATTCTCTGCAATACTACTCAACGTTTTATCAGATC
ATCAAGAACGGTTCCTGTTCAAAAGCCATCTGTACCCTCTTCTAAGCCTAGTTTCTATTGTGGT
ACTCAA
						
					

			
		

Sequences
Splice junction sequence	CTGCAATACTACTCAACGTTTTATCAGATCATCAAGAACGGTTCCTGTTCAAAAGCCATCTGTA CCCTCTTCTAAGCCTAGTTTCTATTGTGGTACTCAAgtggatcattgttttgatatatttgtta caaaattatgtgaaaccattttcacatattacaaaagttgggcggcatggtatgctcattattt ctctggct
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GTGGATCATTGTTTTGATATATTTGTTACAAAATTATGTGAAACCATTTTCACATATTACAAAA GTTGGGCGGCATGGTATGCTCATTATTTCTCTGGCTTATTCAGCTTTTCTTTTGATTATTTCCA TGGACAAACCTTTTTTCTTACTGATGGAGGCCTAAATCATGCAGTGAATTGCTTGATCCTTCAT TCCTCTTTGCTTCGGACAATGCAGAAAAGTATGCCGTGCAACCCATAAGGCTCAATATGCTGCT TAAAATGACTAGAGTGAAGGTGAATGAATTACACTATCATAATTTTTATTTTCCATGATTAGAT TTGGTGCATTAAAGTGTAAACTGGGTGTATGCTACCAGATTGATAATTTTTTGATATGAAAATA GCCCAAGGTGCAGGGATTGAAAATATATGTACATGCTATTAGATACCCATCTCCCAGCAGGGTT TGGAAATGCTTGACTGAAGCCTTAGTTTAAGTGAACAAAGCTTAACTGTTTCATTACTTCTTTA TTTAGTATTTATCAGGGTGTTAACAATTCAAATCTCATTATCTTTTCAAGTGATCTTGGAATCA TGTGACTTACTATAACTTCAGGAATTATACAAAACAGATGTCTTTGTGTACTATTATAAGCAGG AATAAGTTTTTAGAATATTTTTCTTCCTAAGAATTTAATATTTTACTGGTTATCAGTTAGTTGC ATTTGCATCAAAATCTTCCCACAAGTTAGCAAAGCTTGTTGATCAGAAAGTCCAAGTTTGAAAT CTTGTGAATGGAAAAACAGGTGCTGGGAGAGTGTTGCCTGTCTAATTGGTTCAACCCAACTCAA CTTGGATTAGTTAAGGTCCAAGGTGGCTTTTGGATGTTTGGTCTATCTTTTTGGAGCTTCTCCT CTCTTTGAAAGGAGAAACCAGGCCAAATACAATTCTAAAAAAACTATTAAGTATAAAAGTGGCT TTTTTCTATATGAACTAAGGAAGAAAACATAAAAAAAGAAGAGCTTTTAGATAATCTATATTTT AGGAACTTCTGCTTTCCCTTCTTCACTTTTCCAAATCCCAAAACTCAGTAGTGTGCTAACCAAG AATTATTAACTAAAAATTTACTTTTAAGCTGAACGAATATAAAATTATCAAACACATTCAATTA TAAAGTTTTATTTTTACTTGAACTTTAAAGTAGTTTTTAAGATCCAAAAAAATCAATCCAAAGG CCACCTAGTGAAAAAGTTCTCTAAAGTTATTGACGGAGAGAATAAAGACATCTTGGTAGGTGTT TTACTTTTCAATATTTTGTTTCTAGGGTAATTCCATTTTAATCATTAATAAAATTCTTGTAGAA ACTTCTGGTGTCAAAAGGATGGAAAGTAGGTTCCGATCATCTTCAATGCCACATCCTGCAACAA ACTAATGGAGATTGTTTAAGAAAATAATAATGAAGGCTAAGATTGATGCTTACATGAATTATAA CTTTTATTTTCATGGTGTATTCGTTTTTCTATTTAGATGATTTCTCTCACACTCACACTCCCAC ACTCACATGTATGTTCTGCTGGATGTCATAATTAGAATTTGAGTGAGTTGCAATTACTCTCATG TGATTAAATATCTTTTGTTGCACAGCTGCTTGGAAGGATGATTAATTTGCGAAGTTTGATCACT GAACGGATGAACAAGGTTTTCCGTGAAAATATTGAGTTTTTATTTGATCGTTTTGAGTGTCAGG ATCTGTGTGCCATAGTGGTTAGTGATTTTACCTTTGCTACTTCCCTGCCCAACAATTATGAACT ACTTTTTCTTTTCTCAATGTTCATTAACTAATGTAATACTGACTATTTTATTTCAGGAATTAGA GAAGTTGTTGGATGTCTTAAAACATTCCCATGAACTACTGTCTAGAGATCTTTCTGTAGACTCT TTTAGTCTCATGTTGAATGAGATGCAAGAGAACATTTCCCTTGTATCATTTTCTAGTCGATTGG CCTCACAGGTTTGCCCCTTTGAGCTCTAAAGATGTACTTTAATTTTGATGTCTTCCTGTTATTT TTTTAATCTTGAATCCTCATATTGAAAGTTTGAAACCACTATTTGAATGGTAATTAATAATTTC ATGACTTGATCTGTTTCCTACAATTTCATTGATATATTTCTTTACGTATGCTAGATTTGGTCTG AGATGCATAGTGATTTTTTGCCAAATTTCATTCTCTGCAATACTACTCAACGTTTTATCAGATC ATCAAGAACGGTTCCTGTTCAAAAGCCATCTGTACCCTCTTCTAAGCCTAGTTTCTATTGTGGT ACTCAA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.020610498

Conservation Information
Conserved circRNAs	NA
PMCS	0.020610498

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							49659566-49661623(+)
49661254-49659553(+)
49659327-49661560(-)
						
						Potential amino acid sequence
						
							MLLKMTRVKLLGRMINLRSLITERMNKVFRENIEFLFDRFECQDLCAIVELEKLLDVLKHSHEL
LSRDLSVDSFSLMLNEMQENISLVSFSSRLASQIWSEMHSDFLPNFILCNTTQRFIRSSRTVPV
QKPSVPSSKPSFYCGTQVDHCFDIFVTKLCETIFTYYKSWAACELLDPSFLFASDNAEKYAVQP
IRLNMLLKMTRVKLLGRMINLRSLITERMNKVFRENIEFLFDRFECQDLCAIVELEKLLDVLKH
SHELLSRDLSVDSFSLMLNEMQENISLVSFSSRLASQIWSEMHSDFLPNFILCNTTQRFIRSSR
TVPVQKPSVPSSKPSFYCGTQVDHCFDIFVTKLCETIFTYYKSWAACELLDPSFLFASDNAEKY
AVQPIRLNMLLKMTRVKLLGRMINLRSLITERMNKVFRENIEFLFDRFECQDLCAIVELEKLLD
VLKHSHELLSRDLSVDSFSLMLNEMQENISLVSFSSRLASQIWSEMHSDFLPNFILCNTTQRFI
RSSRTVPVQKPSVPSSKPSFYCGTQVDHCFDIFVTKLCETIFTYYKSWAACELLDPSFLFASDN
AEKYAVQPIRLNMLLKMTRVKLLGRMINLRSLITERMNKVFRENIEFLFDRFECQDLCAIVELE
KLLDVLKHSHELLSRDLSVDSFSLMLNEMQENISLVSFSSRLASQIWSEMHSDFLPNFILCNTT
QRFIRSSRTVPVQKPSVPSSKPSFYCGTQ(+)
MRCKRTFPLYHFLVDWPHRFGLRCIVIFCQISFSAILLNVLSDHQERFLFKSHLYPLLSLVSIV
VLKWIIVLIYLLQNYVKPFSHITKVGRHVNCLILHSSLLRTMQKSMPCNP*(+)
MIHLSTTIETRLRRGYRWLLNRNRS*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	49659566-49661623(+) 49661254-49659553(+) 49659327-49661560(-)
Potential amino acid sequence	MLLKMTRVKLLGRMINLRSLITERMNKVFRENIEFLFDRFECQDLCAIVELEKLLDVLKHSHEL LSRDLSVDSFSLMLNEMQENISLVSFSSRLASQIWSEMHSDFLPNFILCNTTQRFIRSSRTVPV QKPSVPSSKPSFYCGTQVDHCFDIFVTKLCETIFTYYKSWAACELLDPSFLFASDNAEKYAVQP IRLNMLLKMTRVKLLGRMINLRSLITERMNKVFRENIEFLFDRFECQDLCAIVELEKLLDVLKH SHELLSRDLSVDSFSLMLNEMQENISLVSFSSRLASQIWSEMHSDFLPNFILCNTTQRFIRSSR TVPVQKPSVPSSKPSFYCGTQVDHCFDIFVTKLCETIFTYYKSWAACELLDPSFLFASDNAEKY AVQPIRLNMLLKMTRVKLLGRMINLRSLITERMNKVFRENIEFLFDRFECQDLCAIVELEKLLD VLKHSHELLSRDLSVDSFSLMLNEMQENISLVSFSSRLASQIWSEMHSDFLPNFILCNTTQRFI RSSRTVPVQKPSVPSSKPSFYCGTQVDHCFDIFVTKLCETIFTYYKSWAACELLDPSFLFASDN AEKYAVQPIRLNMLLKMTRVKLLGRMINLRSLITERMNKVFRENIEFLFDRFECQDLCAIVELE KLLDVLKHSHELLSRDLSVDSFSLMLNEMQENISLVSFSSRLASQIWSEMHSDFLPNFILCNTT QRFIRSSRTVPVQKPSVPSSKPSFYCGTQ(+) MRCKRTFPLYHFLVDWPHRFGLRCIVIFCQISFSAILLNVLSDHQERFLFKSHLYPLLSLVSIV VLKWIIVLIYLLQNYVKPFSHITKVGRHVNCLILHSSLLRTMQKSMPCNP(+) MIHLSTTIETRLRRGYRWLLNRNRS(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017a

circRNA

Detail information of GLYMA_19G250600_circ_g.7

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