Plant Circular RNA Database

Detail information of Os03g0294200_circ_g.5

			
						 General Information
					
						CircRNA Name
						Os03g0294200_circ_g.5
					
						ID in PlantcircBase
						osa_circ_019335
					
						Alias
						Os_ciR8430
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 10264672-10266270  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os03g0294200						
					
						Parent gene annotation
						
							Similar to Fructose-6-phosphate-2-kinase/fructose-2, 6-bisphosph
atase. (Os03t0294200-01);Similar to F2KP (FRUCTOSE-2,6-BISPHOSPH
ATASE); fructose-2,6-bisphosphate 2-phosphatase. (Os03t0294200-0
2)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os03g0294200_circ_g.3 Os03g0294200_circ_g.4 Os03g0294200_circ_g.6 Os03g0294200_circ_g.7 						
					
						Support reads
						2/1
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0294200-02:2
Os03t0294200-01:2

General Information
CircRNA Name	Os03g0294200_circ_g.5
ID in PlantcircBase	osa_circ_019335
Alias	Os_ciR8430
Organism	Oryza sativa
Position	chr3: 10264672-10266270 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os03g0294200
Parent gene annotation	Similar to Fructose-6-phosphate-2-kinase/fructose-2, 6-bisphosph atase. (Os03t0294200-01);Similar to F2KP (FRUCTOSE-2,6-BISPHOSPH ATASE); fructose-2,6-bisphosphate 2-phosphatase. (Os03t0294200-0 2)
Parent gene strand	+
Alternative splicing	Os03g0294200_circ_g.3 Os03g0294200_circ_g.4 Os03g0294200_circ_g.6 Os03g0294200_circ_g.7
Support reads	2/1
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0294200-02:2 Os03t0294200-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CTTTACAGATCATCTTTTTGGAAACAATATGTAATGACCAAAATGTTCTCGAGAGAAATATGCG
CTTGAAAGTTCAACGAAGTCCTGATTATGCGGAACAgtaggtatctttgatgccacaaacagca
caagaaaccggagaaatatgctgatgaaaatggcggaaggcaagtgtaaggtataactgaataa
gtgtgcag

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 170
Transcript exons: 10264672-10264749,10266179-10266270
GTAGGTATCTTTGATGCCACAAACAGCACAAGAAACCGGAGAAATATGCTGATGAAAATGGCGG
AAGGCAAGTGTAAGATCATCTTTTTGGAAACAATATGTAATGACCAAAATGTTCTCGAGAGAAA
TATGCGCTTGAAAGTTCAACGAAGTCCTGATTATGCGGAACA
						
					

						Genomic sequence
						
							GTAGGTATCTTTGATGCCACAAACAGCACAAGAAACCGGAGAAATATGCTGATGAAAATGGCGG
AAGGCAAGTGTAAGGTATAACTGAATAAGTGTGCAGTTCATTGCCTTTTTCTTGGTTGTTTGAA
TCATTTGTTTCTCACCATTGACATTGTTGGAAATTGTCCATGCAGTAATAACTGATTCTTTGTT
CAATTGGTTAGATTTATGTATCACCTTTACACTTTTCATTACAATGATCCACCGGCCACATTGC
CAGCATCGAAAAAATATCACACTTTGTATTATTAGTGACAGGTAGCACATTGCAAATGTACTCT
AGGGGCAAATGTTGTTCTAATCCTTGTTAGCATTTTTGTTCATGTGCTAGTCTCCTAAATTAAT
AATTGTACCTATAATGAATATAAACAGAAAAAGGCGCACTTATAAAGCTTACTCTTGTACAGTA
AGTCAGCAGAAATTAAGAAAATCTACTCAATGGTTCAAACATTTTTTTTTTGTACATTCTCATA
AGATACTGCCCTCGGTGACAACAAAGTCAACTCCAATTGTCTAAGCATTCAGTATAGTGATGCT
AAGACTAGTTTCTTTATAGGAACAGATTCCTGTAAAAAACCAGTTTACGAAAGCCACCACATGA
ACAGAGACATCAGCAGACTTTTGTCTTATACGGCTCACAGACAACAGCTTATCCCTTCATGTCG
TTATGTATGATAAGTCATGCAAAGCAAGTTAACCCTTCTCTATTTTCCATGGCTAGAACCATGC
AGAGCATCAACCTTCTCATCCTACCATCAACATCTAATGAACTCGGACTTATCATGCAGAGATA
TCTCTGGCCAAAACTCTCTCCGGTTCTTGCCGGGTTAACACAGACTTAGAATGCATGAGACAGT
TCGAGAAATGGCAATTGTTGGCACTTTGCAGAGACCAATTATAACAATTAGGCCCTTTATGTTC
CTTACTTCCTTTTGTTACCATCCTAATGGAATCCCAGACTAGTGAATCTGTAGTCATGTGAACA
TTTTTGTTGTGACTTTGCGGGGAAGAGTTAGACGAACTTGTTCAAGATTGGCCATAGTGTAATC
TGACATTTAACTGTGTGGGGCATACAAGGAGGAAAGTTTGTACATTCTTAAGAGGGAAATTTGT
TTTTTTTTTTAAAAAAAAATTACTAGCATTTGATGTGAACGTTTCAAAGGACTGAAGGTTACTT
CATGCTGTTATTGTGATATGGCATGTGAAATATAGGTTTCTATGATCGCATTTACATATCTGGT
TAATTTTGTGATGACAATGGCATCAAACGATTTTTCTGTTAGGATTTACACTATATGCATCCAT
GGTGATAGATACAATCATTTAAGCTACTACTTTCTAGGTTTGGGTGTTTGGGGACTTGTTGATT
TGTTAGATACAATCATTTAAGCTATTACTTTCTAGGTGTGGGTCTTTGGGAACTTGTTGATTTG
TTTTTAACACATGGGTATTTCCCTTTACTTTACAGATCATCTTTTTGGAAACAATATGTAATGA
CCAAAATGTTCTCGAGAGAAATATGCGCTTGAAAGTTCAACGAAGTCCTGATTATGCGGAACA
						
					

			
		

Sequences
Splice junction sequence	CTTTACAGATCATCTTTTTGGAAACAATATGTAATGACCAAAATGTTCTCGAGAGAAATATGCG CTTGAAAGTTCAACGAAGTCCTGATTATGCGGAACAgtaggtatctttgatgccacaaacagca caagaaaccggagaaatatgctgatgaaaatggcggaaggcaagtgtaaggtataactgaataa gtgtgcag
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 170 Transcript exons: 10264672-10264749,10266179-10266270 GTAGGTATCTTTGATGCCACAAACAGCACAAGAAACCGGAGAAATATGCTGATGAAAATGGCGG AAGGCAAGTGTAAGATCATCTTTTTGGAAACAATATGTAATGACCAAAATGTTCTCGAGAGAAA TATGCGCTTGAAAGTTCAACGAAGTCCTGATTATGCGGAACA
Genomic sequence	GTAGGTATCTTTGATGCCACAAACAGCACAAGAAACCGGAGAAATATGCTGATGAAAATGGCGG AAGGCAAGTGTAAGGTATAACTGAATAAGTGTGCAGTTCATTGCCTTTTTCTTGGTTGTTTGAA TCATTTGTTTCTCACCATTGACATTGTTGGAAATTGTCCATGCAGTAATAACTGATTCTTTGTT CAATTGGTTAGATTTATGTATCACCTTTACACTTTTCATTACAATGATCCACCGGCCACATTGC CAGCATCGAAAAAATATCACACTTTGTATTATTAGTGACAGGTAGCACATTGCAAATGTACTCT AGGGGCAAATGTTGTTCTAATCCTTGTTAGCATTTTTGTTCATGTGCTAGTCTCCTAAATTAAT AATTGTACCTATAATGAATATAAACAGAAAAAGGCGCACTTATAAAGCTTACTCTTGTACAGTA AGTCAGCAGAAATTAAGAAAATCTACTCAATGGTTCAAACATTTTTTTTTTGTACATTCTCATA AGATACTGCCCTCGGTGACAACAAAGTCAACTCCAATTGTCTAAGCATTCAGTATAGTGATGCT AAGACTAGTTTCTTTATAGGAACAGATTCCTGTAAAAAACCAGTTTACGAAAGCCACCACATGA ACAGAGACATCAGCAGACTTTTGTCTTATACGGCTCACAGACAACAGCTTATCCCTTCATGTCG TTATGTATGATAAGTCATGCAAAGCAAGTTAACCCTTCTCTATTTTCCATGGCTAGAACCATGC AGAGCATCAACCTTCTCATCCTACCATCAACATCTAATGAACTCGGACTTATCATGCAGAGATA TCTCTGGCCAAAACTCTCTCCGGTTCTTGCCGGGTTAACACAGACTTAGAATGCATGAGACAGT TCGAGAAATGGCAATTGTTGGCACTTTGCAGAGACCAATTATAACAATTAGGCCCTTTATGTTC CTTACTTCCTTTTGTTACCATCCTAATGGAATCCCAGACTAGTGAATCTGTAGTCATGTGAACA TTTTTGTTGTGACTTTGCGGGGAAGAGTTAGACGAACTTGTTCAAGATTGGCCATAGTGTAATC TGACATTTAACTGTGTGGGGCATACAAGGAGGAAAGTTTGTACATTCTTAAGAGGGAAATTTGT TTTTTTTTTTAAAAAAAAATTACTAGCATTTGATGTGAACGTTTCAAAGGACTGAAGGTTACTT CATGCTGTTATTGTGATATGGCATGTGAAATATAGGTTTCTATGATCGCATTTACATATCTGGT TAATTTTGTGATGACAATGGCATCAAACGATTTTTCTGTTAGGATTTACACTATATGCATCCAT GGTGATAGATACAATCATTTAAGCTACTACTTTCTAGGTTTGGGTGTTTGGGGACTTGTTGATT TGTTAGATACAATCATTTAAGCTATTACTTTCTAGGTGTGGGTCTTTGGGAACTTGTTGATTTG TTTTTAACACATGGGTATTTCCCTTTACTTTACAGATCATCTTTTTGGAAACAATATGTAATGA CCAAAATGTTCTCGAGAGAAATATGCGCTTGAAAGTTCAACGAAGTCCTGATTATGCGGAACA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.215156171

Conservation Information
Conserved circRNAs	NA
PMCS	0.215156171

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							10264718-10264672(+)
10266185-10266182(-)
						
						Potential amino acid sequence
						
							MLMKMAEGKCKIIFLETICNDQNVLERNMRLKVQRSPDYAEQ*(+)
MILHLPSAIFISIFLRFLVLFVASKIPTVPHNQDFVELSSAYFSREHFGHYILFPKR*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	10264718-10264672(+) 10266185-10266182(-)
Potential amino acid sequence	MLMKMAEGKCKIIFLETICNDQNVLERNMRLKVQRSPDYAEQ(+) MILHLPSAIFISIFLRFLVLFVASKIPTVPHNQDFVELSSAYFSREHFGHYILFPKR(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os03g0294200_circ_g.5

Address

Any questions?

Supported by