Plant Circular RNA Database

Detail information of Os01g0834200_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os01g0834200_circ_g.4
					
						ID in PlantcircBase
						osa_circ_004722
					
						Alias
						Os_ciR4514
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 35736580-35737237  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Os01g0834200						
					
						Parent gene annotation
						
							Similar to T-cell immune regulator 1 transcript variant 3 (Fragm
ent). (Os01t0834200-01);Similar to VHA-A1 (VACUOLAR PROTON ATPAS
E A 1); ATPase. (Os01t0834200-02);Similar to T-cell immune regul
ator 1 transcript variant 3 (Fragment). (Os01t0834200-03);Simila
r to VHA-A1 (VACUOLAR PROTON ATPASE A 1); ATPase. (Os01t0834200-
04)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os01g0834200_circ_g.2 Os01g0834200_circ_g.3 Os01g0834200_circ_g.5 Os01g0834200_circ_g.6 Os01g0834200_circ_g.7 Os01g0834200_circ_g.8 Os01g0834200_circ_g.9 Os01g0834200_circ_g.10 Os01g0834200_circ_g.11 						
					
						Support reads
						4
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0834200-03:3
Os01t0834200-01:3
Os01t0834200-02:3

General Information
CircRNA Name	Os01g0834200_circ_g.4
ID in PlantcircBase	osa_circ_004722
Alias	Os_ciR4514
Organism	Oryza sativa
Position	chr1: 35736580-35737237 JBrowse»
Reference genome	IRGSP-1.0.38
Type	u-circRNA
Identification method	find_circ
Parent gene	Os01g0834200
Parent gene annotation	Similar to T-cell immune regulator 1 transcript variant 3 (Fragm ent). (Os01t0834200-01);Similar to VHA-A1 (VACUOLAR PROTON ATPAS E A 1); ATPase. (Os01t0834200-02);Similar to T-cell immune regul ator 1 transcript variant 3 (Fragment). (Os01t0834200-03);Simila r to VHA-A1 (VACUOLAR PROTON ATPASE A 1); ATPase. (Os01t0834200- 04)
Parent gene strand	+
Alternative splicing	Os01g0834200_circ_g.2 Os01g0834200_circ_g.3 Os01g0834200_circ_g.5 Os01g0834200_circ_g.6 Os01g0834200_circ_g.7 Os01g0834200_circ_g.8 Os01g0834200_circ_g.9 Os01g0834200_circ_g.10 Os01g0834200_circ_g.11
Support reads	4
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0834200-03:3 Os01t0834200-01:3 Os01t0834200-02:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AGAGAAAGCTGTATATGATACATTGAACATGCTGAATTTTGATGTCACGAAGAAATGCCTTGTT
GGGGAAGGATGGTGTCCTATATTTGCTAAATCACAGgtagagaaaactgttttcgtagttttct
tttctggggatcaggctaaagcaaaaatattaaagatttgcggttcatttggagcaagttgcta
ccctgttc

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GTAGAGAAAACTGTTTTCGTAGTTTTCTTTTCTGGGGATCAGGCTAAAGCAAAAATATTAAAGA
TTTGCGGTTCATTTGGAGCAAGTTGCTACCCTGTTCCCGAGGAGATGGTTAAGCAGAGACAGAT
TTTTCGCGAGGTACTTTAATTTGCATATTTGGCACACAAATTCACTGCTTGATTTGACCGTGCT
TGTTAGAATTTTATTGGAAGAAGCTTGAGTGTTATACTTATCTGACTTATTGTTCTCGTTATTT
TGTGTCATAATGTTAATATGTTATCTGCTGAGATATTATGTAGTAGTAACGGTTTATGAAAATG
TGTTAGGTATCTGGACGCCTTGCTGATCTGGAAGCCACTTTGGATGCTGGAATCCAGCACAGAA
ACAAAGCACTAGAGTCAGTAGGTTCACAATTATGGAGGTGGACAATCATGGTAATGAAGTAATA
TAAAATTTTCATTTTCTATTTGTGCGTTGTAATCCCTAAAATCTCTTATTTATGTCTGTTCTGA
TACATGGATTTCTGAACCATAACCCCCTTTTGTGTTAGGTGAAAAAAGAGAAAGCTGTATATGA
TACATTGAACATGCTGAATTTTGATGTCACGAAGAAATGCCTTGTTGGGGAAGGATGGTGTCCT
ATATTTGCTAAATCACAG

Sequences
Splice junction sequence	AGAGAAAGCTGTATATGATACATTGAACATGCTGAATTTTGATGTCACGAAGAAATGCCTTGTT GGGGAAGGATGGTGTCCTATATTTGCTAAATCACAGgtagagaaaactgttttcgtagttttct tttctggggatcaggctaaagcaaaaatattaaagatttgcggttcatttggagcaagttgcta ccctgttc
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GTAGAGAAAACTGTTTTCGTAGTTTTCTTTTCTGGGGATCAGGCTAAAGCAAAAATATTAAAGA TTTGCGGTTCATTTGGAGCAAGTTGCTACCCTGTTCCCGAGGAGATGGTTAAGCAGAGACAGAT TTTTCGCGAGGTACTTTAATTTGCATATTTGGCACACAAATTCACTGCTTGATTTGACCGTGCT TGTTAGAATTTTATTGGAAGAAGCTTGAGTGTTATACTTATCTGACTTATTGTTCTCGTTATTT TGTGTCATAATGTTAATATGTTATCTGCTGAGATATTATGTAGTAGTAACGGTTTATGAAAATG TGTTAGGTATCTGGACGCCTTGCTGATCTGGAAGCCACTTTGGATGCTGGAATCCAGCACAGAA ACAAAGCACTAGAGTCAGTAGGTTCACAATTATGGAGGTGGACAATCATGGTAATGAAGTAATA TAAAATTTTCATTTTCTATTTGTGCGTTGTAATCCCTAAAATCTCTTATTTATGTCTGTTCTGA TACATGGATTTCTGAACCATAACCCCCTTTTGTGTTAGGTGAAAAAAGAGAAAGCTGTATATGA TACATTGAACATGCTGAATTTTGATGTCACGAAGAAATGCCTTGTTGGGGAAGGATGGTGTCCT ATATTTGCTAAATCACAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.203550621

Conservation Information
Conserved circRNAs	NA
PMCS	0.203550621

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							35736689-35737234(+)
35737180-35736580(+)
						
						Potential amino acid sequence
						
							MVKQRQIFREVSGRLADLEATLDAGIQHRNKALESVGSQLWRWTIMVKKEKAVYDTLNMLNFDV
TKKCLVGEGWCPIFAKSQVEKTVFVVFFSGDQAKAKILKICGSFGASCYPVPEEMVKQRQIFRE
VSGRLADLEATLDAGIQHRNKALESVGSQLWRWTIMVKKEKAVYDTLNMLNFDVTKKCLVGEGW
CPIFAKSQVEKTVFVVFFSGDQAKAKILKICGSFGASCYPVPEEMVKQRQIFREVSGRLADLEA
TLDAGIQHRNKALESVGSQLWRWTIMVKKEKAVYDTLNMLNFDVTKKCLVGEGWCPIFAKSQVE
KTVFVVFFSGDQAKAKILKICGSFGASCYPVPEEMVKQRQIFREVSGRLADLEATLDAGIQHRN
KALESVGSQLWRWTIMVKKEKAVYDTLNMLNFDVTKKCLVGEGWCPIFAKSQ(+)
MSRRNALLGKDGVLYLLNHR*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	35736689-35737234(+) 35737180-35736580(+)
Potential amino acid sequence	MVKQRQIFREVSGRLADLEATLDAGIQHRNKALESVGSQLWRWTIMVKKEKAVYDTLNMLNFDV TKKCLVGEGWCPIFAKSQVEKTVFVVFFSGDQAKAKILKICGSFGASCYPVPEEMVKQRQIFRE VSGRLADLEATLDAGIQHRNKALESVGSQLWRWTIMVKKEKAVYDTLNMLNFDVTKKCLVGEGW CPIFAKSQVEKTVFVVFFSGDQAKAKILKICGSFGASCYPVPEEMVKQRQIFREVSGRLADLEA TLDAGIQHRNKALESVGSQLWRWTIMVKKEKAVYDTLNMLNFDVTKKCLVGEGWCPIFAKSQVE KTVFVVFFSGDQAKAKILKICGSFGASCYPVPEEMVKQRQIFREVSGRLADLEATLDAGIQHRN KALESVGSQLWRWTIMVKKEKAVYDTLNMLNFDVTKKCLVGEGWCPIFAKSQ(+) MSRRNALLGKDGVLYLLNHR*(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of Os01g0834200_circ_g.4

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