Plant Circular RNA Database

Detail information of Os08g0323600_circ_g.6

			
						 General Information
					
						CircRNA Name
						Os08g0323600_circ_g.6
					
						ID in PlantcircBase
						osa_circ_036725
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr8: 14176523-14178143  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os08g0323600						
					
						Parent gene annotation
						
							Hypothetical conserved gene. (Os08t0323600-01);Ribonuclease E an
d G domain containing protein. (Os08t0323600-02);Similar to Ribo
nuclease E. (Os08t0323600-03)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os08g0323600_circ_g.1 Os08g0323600_circ_g.2 Os08g0323600_circ_g.3 Os08g0323600_circ_g.4 Os08g0323600_circ_g.5 Os08g0323600_circ_g.7 						
					
						Support reads
						1
					
						Tissues
						shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os08t0323600-02:2
Os08t0323600-01:2

General Information
CircRNA Name	Os08g0323600_circ_g.6
ID in PlantcircBase	osa_circ_036725
Alias	NA
Organism	Oryza sativa
Position	chr8: 14176523-14178143 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os08g0323600
Parent gene annotation	Hypothetical conserved gene. (Os08t0323600-01);Ribonuclease E an d G domain containing protein. (Os08t0323600-02);Similar to Ribo nuclease E. (Os08t0323600-03)
Parent gene strand	-
Alternative splicing	Os08g0323600_circ_g.1 Os08g0323600_circ_g.2 Os08g0323600_circ_g.3 Os08g0323600_circ_g.4 Os08g0323600_circ_g.5 Os08g0323600_circ_g.7
Support reads	1
Tissues	shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os08t0323600-02:2 Os08t0323600-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							AGTAGACATCAATGGCTTGGGGGAATGAAGTATGTTTGGTTCCACTTGAAGATTGCCACCTCTA
AGAGCATCCATCAATTGTACTGAACACAAAGTATGCctttatttcggccgtccataagttgtta
ctttcaacagaaggagacaactgaacagccatatctgattcccaacagcccaaggtaactggat
ctccagtg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 292
Transcript exons: 14176523-14176707,14178037-14178143
CTTTATTTCGGCCGTCCATAAGTTGTTACTTTCAACAGAAGGAGACAACTGAACAGCCATATCT
GATTCCCAACAGCCCAAGGTAACTGGATCTCCAGTGACGAAGATTATGTAACCATCCAGCACAT
CTGATTTGAGGTTCCACGATATTGTACAGAATCCTTTACAGGTTATGGCTGAGTCATCACGTCT
AGTAGACATCAATGGCTTGGGGGAATGAAGTATGTTTGGTTCCACTTGAAGATTGCCACCTCTA
AGAGCATCCATCAATTGTACTGAACACAAAGTATGC
						
					

						Genomic sequence
						
							CTTTATTTCGGCCGTCCATAAGTTGTTACTTTCAACAGAAGGAGACAACTGAACAGCCATATCT
GATTCCCAACAGCCCAAGGTAACTGGATCTCCAGTGACGAAGATTATGTAACCATCCAGCACAT
CTGATTTGAGGTTCCACGATATTGTACAGAATCCTTTACAGGTTATGGCTGAGTCATCTGTAAG
AAACCAACTGGGAAATGACATAAACTTCAAAACACGAACTATATGTCTATATGGTAAAATTTTA
GTTGCATACCTTGTTCAATTTCATATTATAATATAAATTTGAGAGGTCAAAAGTGTATACTAGC
AGTGAACAAAACTTTGGTTGCATGAACTATATATCTAAATGATAAACTCTTAGTTGCATGGACA
GCATGGTTTACTAAAACAAACTTTGCACATGTAGGGGAAAGGATGCTCTTCAGTACACTTCTTC
CAACTTCTACGAAGCTGAACAAAGTGGCCGATTTTACTAAACACCTGAGCTGAACTTCTATGAT
CCTTGTGTGTGTAATATAACTTGAACATGTCAAAACTATGTATTAGTCTCGGAAAGAGTGCATG
ATTTTAGCAAATAAGACTTGCACAAATATAGGGGAAGGAATGTGCTTTGATTCACTTCTTTCAA
AGGAAATAGTCTATGTTCGGGTGAGCAAATCTGCATGAACTTCAAAATTAAAAGATCTATATCC
ATACATAATCTTTGATGTGACACTTTTATGTTAATATAGGTTGGTCAACCTAAAAATAACCTGT
TTTCTTTAAGATTCGTTAACTTTTAAAAATAGGTTTGCCTAAGCAAGGCCCTTGGTAGAATTGC
CAACCATTCAGGACTTAGTTTAGATTTTAAGGATTTGGGTTCAACCCATCGAAACAGGCCCTTT
AGCAATTAGAAAAGGTACACTTTTGAGAAACAAAGATGTACATTTCACTAGGTTTTACGCCTGT
AGTTTAACAAGAAAAATGAATAGCAAATGAAAAGATGAATGCAGTAACATACATGTTGATGAAA
TATCCCTCAGTAACAATAATTAATGTTTTAGTGGTGCATGTGAGACAACACAAGAAATCCCCTC
CAATCTGAAGGCAAAAGACATGTTTCTTCCAAATATTTCCTTGTATTGCTGTTTAGTGTTTTAC
ATTTCATGTTACAGATGAATGAATACATAATGAATTGGCGGAATACATTTTCATACTCTCAATT
GCAGCTATCAGGATAGAGCCTAAATGAGAGAGGTCACCTCTATTTTTCTAATATAAACAGCTGC
GTCAGTCATGAATTTTTTGCTTGTGTAAAAGATGATATTCATTCCAATTATCTAGGCAACTAAA
CAAGACGTACAACTTTATTTGTTAAAATTGCTGAAATGTATGATTAAAAAATTTCCTGGGAAAT
GCAACTGATGTTCTATGCAGGAACCGCACACTCATTATATATTCATAAAATAAAAAATCCCAAA
TACATTTCAAGTGTATATGGACAACTAAACTAAAGAAATTACCACGTCTAGTAGACATCAATGG
CTTGGGGGAATGAAGTATGTTTGGTTCCACTTGAAGATTGCCACCTCTAAGAGCATCCATCAAT
TGTACTGAACACAAAGTATGC
						
					

			
		

Sequences
Splice junction sequence	AGTAGACATCAATGGCTTGGGGGAATGAAGTATGTTTGGTTCCACTTGAAGATTGCCACCTCTA AGAGCATCCATCAATTGTACTGAACACAAAGTATGCctttatttcggccgtccataagttgtta ctttcaacagaaggagacaactgaacagccatatctgattcccaacagcccaaggtaactggat ctccagtg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 292 Transcript exons: 14176523-14176707,14178037-14178143 CTTTATTTCGGCCGTCCATAAGTTGTTACTTTCAACAGAAGGAGACAACTGAACAGCCATATCT GATTCCCAACAGCCCAAGGTAACTGGATCTCCAGTGACGAAGATTATGTAACCATCCAGCACAT CTGATTTGAGGTTCCACGATATTGTACAGAATCCTTTACAGGTTATGGCTGAGTCATCACGTCT AGTAGACATCAATGGCTTGGGGGAATGAAGTATGTTTGGTTCCACTTGAAGATTGCCACCTCTA AGAGCATCCATCAATTGTACTGAACACAAAGTATGC
Genomic sequence	CTTTATTTCGGCCGTCCATAAGTTGTTACTTTCAACAGAAGGAGACAACTGAACAGCCATATCT GATTCCCAACAGCCCAAGGTAACTGGATCTCCAGTGACGAAGATTATGTAACCATCCAGCACAT CTGATTTGAGGTTCCACGATATTGTACAGAATCCTTTACAGGTTATGGCTGAGTCATCTGTAAG AAACCAACTGGGAAATGACATAAACTTCAAAACACGAACTATATGTCTATATGGTAAAATTTTA GTTGCATACCTTGTTCAATTTCATATTATAATATAAATTTGAGAGGTCAAAAGTGTATACTAGC AGTGAACAAAACTTTGGTTGCATGAACTATATATCTAAATGATAAACTCTTAGTTGCATGGACA GCATGGTTTACTAAAACAAACTTTGCACATGTAGGGGAAAGGATGCTCTTCAGTACACTTCTTC CAACTTCTACGAAGCTGAACAAAGTGGCCGATTTTACTAAACACCTGAGCTGAACTTCTATGAT CCTTGTGTGTGTAATATAACTTGAACATGTCAAAACTATGTATTAGTCTCGGAAAGAGTGCATG ATTTTAGCAAATAAGACTTGCACAAATATAGGGGAAGGAATGTGCTTTGATTCACTTCTTTCAA AGGAAATAGTCTATGTTCGGGTGAGCAAATCTGCATGAACTTCAAAATTAAAAGATCTATATCC ATACATAATCTTTGATGTGACACTTTTATGTTAATATAGGTTGGTCAACCTAAAAATAACCTGT TTTCTTTAAGATTCGTTAACTTTTAAAAATAGGTTTGCCTAAGCAAGGCCCTTGGTAGAATTGC CAACCATTCAGGACTTAGTTTAGATTTTAAGGATTTGGGTTCAACCCATCGAAACAGGCCCTTT AGCAATTAGAAAAGGTACACTTTTGAGAAACAAAGATGTACATTTCACTAGGTTTTACGCCTGT AGTTTAACAAGAAAAATGAATAGCAAATGAAAAGATGAATGCAGTAACATACATGTTGATGAAA TATCCCTCAGTAACAATAATTAATGTTTTAGTGGTGCATGTGAGACAACACAAGAAATCCCCTC CAATCTGAAGGCAAAAGACATGTTTCTTCCAAATATTTCCTTGTATTGCTGTTTAGTGTTTTAC ATTTCATGTTACAGATGAATGAATACATAATGAATTGGCGGAATACATTTTCATACTCTCAATT GCAGCTATCAGGATAGAGCCTAAATGAGAGAGGTCACCTCTATTTTTCTAATATAAACAGCTGC GTCAGTCATGAATTTTTTGCTTGTGTAAAAGATGATATTCATTCCAATTATCTAGGCAACTAAA CAAGACGTACAACTTTATTTGTTAAAATTGCTGAAATGTATGATTAAAAAATTTCCTGGGAAAT GCAACTGATGTTCTATGCAGGAACCGCACACTCATTATATATTCATAAAATAAAAAATCCCAAA TACATTTCAAGTGTATATGGACAACTAAACTAAAGAAATTACCACGTCTAGTAGACATCAATGG CTTGGGGGAATGAAGTATGTTTGGTTCCACTTGAAGATTGCCACCTCTAAGAGCATCCATCAAT TGTACTGAACACAAAGTATGC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.224680084

Conservation Information
Conserved circRNAs	NA
PMCS	0.224680084

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							14178141-14176605(+)
14178120-14178107(-)
						
						Potential amino acid sequence
						
							MPLFRPSISCYFQQKETTEQPYLIPNSPR*(+)
MDALRGGNLQVEPNILHSPKPLMSTRRDDSAITCKGFCTISWNLKSDVLDGYIIFVTGDPVTLG
CWESDMAVQLSPSVESNNLWTAEIKAYFVFSTIDGCS*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	14178141-14176605(+) 14178120-14178107(-)
Potential amino acid sequence	MPLFRPSISCYFQQKETTEQPYLIPNSPR(+) MDALRGGNLQVEPNILHSPKPLMSTRRDDSAITCKGFCTISWNLKSDVLDGYIIFVTGDPVTLG CWESDMAVQLSPSVESNNLWTAEIKAYFVFSTIDGCS(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os08g0323600_circ_g.6

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