Plant Circular RNA Database

Detail information of Os10g0181333_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os10g0181333_circ_g.3
					
						ID in PlantcircBase
						osa_circ_006324
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr10: 5579932-5580940  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ie-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os10g0181333						
					
						Parent gene annotation
						
							Hypothetical protein. (Os10t0181333-00)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os10g0181333_circ_g.4 Os10g0181333_circ_g.5 Os10g0181333_circ_g.6 Os10g0181333_circ_g.7 Os10g0181333_circ_g.8 Os10g0181333_circ_g.9 Os10g0181333_circ_g.10 Os10g0181333_circ_g.11 Os10g0181333_circ_g.12 Os10g0181333_circ_g.13 Os10g0181333_circ_g.14 						
					
						Support reads
						1
					
						Tissues
						shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os10t0181200-01:3
Os10t0181200-02:2
Os10t0181333-00:2
Os10t0181200-01:3
Os10t0181200-02:2

General Information
CircRNA Name	Os10g0181333_circ_g.3
ID in PlantcircBase	osa_circ_006324
Alias	NA
Organism	Oryza sativa
Position	chr10: 5579932-5580940 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ie-circRNA
Identification method	CIRCexplorer
Parent gene	Os10g0181333
Parent gene annotation	Hypothetical protein. (Os10t0181333-00)
Parent gene strand	+
Alternative splicing	Os10g0181333_circ_g.4 Os10g0181333_circ_g.5 Os10g0181333_circ_g.6 Os10g0181333_circ_g.7 Os10g0181333_circ_g.8 Os10g0181333_circ_g.9 Os10g0181333_circ_g.10 Os10g0181333_circ_g.11 Os10g0181333_circ_g.12 Os10g0181333_circ_g.13 Os10g0181333_circ_g.14
Support reads	1
Tissues	shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os10t0181200-01:3 Os10t0181200-02:2 Os10t0181333-00:2 Os10t0181200-01:3 Os10t0181200-02:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AGGAAACCTTAAACATCCCAACACTTGAGACAAAACATCACTTGAGGGTAATAGTCCTGTGGAA
ATTGCCTCACGGTAGACCTTAATTGCTGAGGAGGTCctgcagctagtcgatgctttaaagacct
caatgttgtaagaactgaaacctgtcaaaaaaaaaaaggaagtatatacgcatgtaatatgtta
taaggaaa

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 374
Transcript exons: 5579932-5579980,5580534-5580626,5580709-5580940
CTGCAGCTAGTCGATGCTTTAAAGACCTCAATGTTGTAAGAACTGAAACCTCCGCAGTGTAGAT
CTTTGACTTTCGGGCATCAACAAGAATACGGGTATCTTTCATGGAAATACTTTCAACTGCTCCT
AACGAAGCAGCTTCCTCCAGTATTGAAAAGGCACGAATGTCGTATTCACCAAAACCTTCCAACA
GTGAATTCACATTGGGATGATGTGGAATATCACAACTTATTCCCATATTTTCAATGAAAGTATT
TGTTAATGTATTATCATGAGGAAACCTTAAACATCCCAACACTTGAGACAAAACATCACTTGAG
GGTAATAGTCCTGTGGAAATTGCCTCACGGTAGACCTTAATTGCTGAGGAGGTC
						
						Genomic sequence
						
							CTGCAGCTAGTCGATGCTTTAAAGACCTCAATGTTGTAAGAACTGAAACCTGTCAAAAAAAAAA
AGGAAGTATATACGCATGTAATATGTTATAAGGAAAGGTTATAAAAGGATAAATGCACTTGGAA
ATCGTCCCCCTGTCATTGACAAAAGCAACAGCCAAGAAGAAATATGTCTGCAAAACTTATACAG
AAGGAAAATACTAATTATTTAGCTCTCATATTCCTCTCCTATGTAGTAAATTAAGAAACAAAAA
AAAAGCATATTTTGTAATACACCACTATGCATAATAAAGTTTTAGGTTCTAAAGATGCCTATCC
ACTATGCAAAGTAGTCGTGCTAATCAGAGAGGGCAGCATTAGTTGAAATACATGGAAAATATGC
AGTTTTGTAAACTAGCAACAAGTATTAAGTACTGTAGTAACCCAGAAAGCATACTTATTCAGCA
CACCAGGTTTATATAACAGAAAGTGGTCAACATAGAACTGGTTTTAACTACAAATAATGTTCTT
AGATACATGAGTACTGCACACTTATGTTGTGACTTATTTATTGAGAAAAATATATTTATTAAAG
CAAGCAAAGTGCAATTAGCAAAATACCTCCGCAGTGTAGATCTTTGACTTTCGGGCATCAACAA
GAATACGGGTATCTTTCATGGAAATACTTTCAACTGCTCCTAACGAAGCAGCTTCCTGTGACAA
GAAGGCATCTGCTTTGATTGATACAAGAATACTGAAATGAAGCCATTGTTTTAGTTCGAATTAG
TATCAGTACCTCCAGTATTGAAAAGGCACGAATGTCGTATTCACCAAAACCTTCCAACAGTGAA
TTCACATTGGGATGATGTGGAATATCACAACTTATTCCCATATTTTCAATGAAAGTATTTGTTA
ATGTATTATCATGAGGAAACCTTAAACATCCCAACACTTGAGACAAAACATCACTTGAGGGTAA
TAGTCCTGTGGAAATTGCCTCACGGTAGACCTTAATTGCTGAGGAGGTC

Sequences
Splice junction sequence	AGGAAACCTTAAACATCCCAACACTTGAGACAAAACATCACTTGAGGGTAATAGTCCTGTGGAA ATTGCCTCACGGTAGACCTTAATTGCTGAGGAGGTCctgcagctagtcgatgctttaaagacct caatgttgtaagaactgaaacctgtcaaaaaaaaaaaggaagtatatacgcatgtaatatgtta taaggaaa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 374 Transcript exons: 5579932-5579980,5580534-5580626,5580709-5580940 CTGCAGCTAGTCGATGCTTTAAAGACCTCAATGTTGTAAGAACTGAAACCTCCGCAGTGTAGAT CTTTGACTTTCGGGCATCAACAAGAATACGGGTATCTTTCATGGAAATACTTTCAACTGCTCCT AACGAAGCAGCTTCCTCCAGTATTGAAAAGGCACGAATGTCGTATTCACCAAAACCTTCCAACA GTGAATTCACATTGGGATGATGTGGAATATCACAACTTATTCCCATATTTTCAATGAAAGTATT TGTTAATGTATTATCATGAGGAAACCTTAAACATCCCAACACTTGAGACAAAACATCACTTGAG GGTAATAGTCCTGTGGAAATTGCCTCACGGTAGACCTTAATTGCTGAGGAGGTC
Genomic sequence	CTGCAGCTAGTCGATGCTTTAAAGACCTCAATGTTGTAAGAACTGAAACCTGTCAAAAAAAAAA AGGAAGTATATACGCATGTAATATGTTATAAGGAAAGGTTATAAAAGGATAAATGCACTTGGAA ATCGTCCCCCTGTCATTGACAAAAGCAACAGCCAAGAAGAAATATGTCTGCAAAACTTATACAG AAGGAAAATACTAATTATTTAGCTCTCATATTCCTCTCCTATGTAGTAAATTAAGAAACAAAAA AAAAGCATATTTTGTAATACACCACTATGCATAATAAAGTTTTAGGTTCTAAAGATGCCTATCC ACTATGCAAAGTAGTCGTGCTAATCAGAGAGGGCAGCATTAGTTGAAATACATGGAAAATATGC AGTTTTGTAAACTAGCAACAAGTATTAAGTACTGTAGTAACCCAGAAAGCATACTTATTCAGCA CACCAGGTTTATATAACAGAAAGTGGTCAACATAGAACTGGTTTTAACTACAAATAATGTTCTT AGATACATGAGTACTGCACACTTATGTTGTGACTTATTTATTGAGAAAAATATATTTATTAAAG CAAGCAAAGTGCAATTAGCAAAATACCTCCGCAGTGTAGATCTTTGACTTTCGGGCATCAACAA GAATACGGGTATCTTTCATGGAAATACTTTCAACTGCTCCTAACGAAGCAGCTTCCTGTGACAA GAAGGCATCTGCTTTGATTGATACAAGAATACTGAAATGAAGCCATTGTTTTAGTTCGAATTAG TATCAGTACCTCCAGTATTGAAAAGGCACGAATGTCGTATTCACCAAAACCTTCCAACAGTGAA TTCACATTGGGATGATGTGGAATATCACAACTTATTCCCATATTTTCAATGAAAGTATTTGTTA ATGTATTATCATGAGGAAACCTTAAACATCCCAACACTTGAGACAAAACATCACTTGAGGGTAA TAGTCCTGTGGAAATTGCCTCACGGTAGACCTTAATTGCTGAGGAGGTC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.258555335

Conservation Information
Conserved circRNAs	NA
PMCS	0.258555335

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							5580806-5580851(-)
						
						Potential amino acid sequence
						
							MGISCDIPHHPNVNSLLEGFGEYDIRAFSILEEAASLGAVESISMKDTRILVDARKSKIYTAEV
SVLTTLRSLKHRLAAGPPQQLRSTVRQFPQDYYPQVMFCLKCWDV*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	5580806-5580851(-)
Potential amino acid sequence	MGISCDIPHHPNVNSLLEGFGEYDIRAFSILEEAASLGAVESISMKDTRILVDARKSKIYTAEV SVLTTLRSLKHRLAAGPPQQLRSTVRQFPQDYYPQVMFCLKCWDV*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os10g0181333_circ_g.3

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