Plant Circular RNA Database

Detail information of Os10g0476600_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os10g0476600_circ_g.2
					
						ID in PlantcircBase
						osa_circ_007097
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr10: 17758710-17759355  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os10g0476600						
					
						Parent gene annotation
						
							Similar to Protein phosphatase 2A 62 kDa B'' regulatory subunit 
(Protein phosphatase 2A 62 kDa B regulatory subunit) (Protein ph
osphatase 2A alpha). (Os10t0476600-01);Similar to Protein phosph
atase 2A 62 kDa B'' regulatory subunit (Protein phosphatase 2A 6
2 kDa B regulatory subunit) (Protein phosphatase 2A alpha). (Os1
0t0476600-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os10g0476600_circ_g.1 Os10g0476600_circ_g.3 Os10g0476600_circ_g.4 Os10g0476600_circ_g.5 Os10g0476600_circ_g.6 Os10g0476600_circ_g.7 Os10g0476600_circ_g.8 Os10g0476600_circ_g.9 Os10g0476600_circ_g.10 Os10g0476600_circ_g.11 Os10g0476600_circ_g.12 Os10g0476600_circ_g.13 Os10g0476600_circ_g.14 Os10g0476600_circ_g.15 						
					
						Support reads
						1
					
						Tissues
						shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os10t0476600-01:3
Os10t0476600-02:3

General Information
CircRNA Name	Os10g0476600_circ_g.2
ID in PlantcircBase	osa_circ_007097
Alias	NA
Organism	Oryza sativa
Position	chr10: 17758710-17759355 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os10g0476600
Parent gene annotation	Similar to Protein phosphatase 2A 62 kDa B'' regulatory subunit (Protein phosphatase 2A 62 kDa B regulatory subunit) (Protein ph osphatase 2A alpha). (Os10t0476600-01);Similar to Protein phosph atase 2A 62 kDa B'' regulatory subunit (Protein phosphatase 2A 6 2 kDa B regulatory subunit) (Protein phosphatase 2A alpha). (Os1 0t0476600-02)
Parent gene strand	+
Alternative splicing	Os10g0476600_circ_g.1 Os10g0476600_circ_g.3 Os10g0476600_circ_g.4 Os10g0476600_circ_g.5 Os10g0476600_circ_g.6 Os10g0476600_circ_g.7 Os10g0476600_circ_g.8 Os10g0476600_circ_g.9 Os10g0476600_circ_g.10 Os10g0476600_circ_g.11 Os10g0476600_circ_g.12 Os10g0476600_circ_g.13 Os10g0476600_circ_g.14 Os10g0476600_circ_g.15
Support reads	1
Tissues	shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os10t0476600-01:3 Os10t0476600-02:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AATTTAGATCAGTCACAAAAGATATATGCAAACTTCCATCGTTCTTCTCCAGTGTTCTTTTCAA
GAAGATCGATGCTGCAGGCTCTGGAACTGTCACAAGgtgaactctttgattgaagatgcaaaag
ctggtgctacattgaatgttactgggagctctgctagcacaaatgctgcttcaagtggttcttt
gccatcaa

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 452
Transcript exons: 17758710-17758958,17759069-17759171,17759256-17759355
GTGAACTCTTTGATTGAAGATGCAAAAGCTGGTGCTACATTGAATGTTACTGGGAGCTCTGCTA
GCACAAATGCTGCTTCAAGTGGTTCTTTGCCATCAATGTTTCCGGCTGGTAGTGCTCCTCCCCT
TTCTCCTAGAAGCACTTCTGGCTCCCCCCGTGTTATGAGGCGAGGATCTGGAGCTGGACCATCA
TCCTTGGGATCTCCACTAAAAGTAGTTAGTGAACCAGTGAGGGAGGTTATACCCCAGTTTTACT
TCAAATATGGGCGCCCTGCTCCAAAAGATTTGAAGGAGCAATGCTTATCTAGAATAGATCACCT
CTTTTTTGCAGGGGAGGGACTCCAGATTCAAGAATTTAGATCAGTCACAAAAGATATATGCAAA
CTTCCATCGTTCTTCTCCAGTGTTCTTTTCAAGAAGATCGATGCTGCAGGCTCTGGAACTGTCA
CAAG
						
						Genomic sequence
						
							GTGAACTCTTTGATTGAAGATGCAAAAGCTGGTGCTACATTGAATGTTACTGGGAGCTCTGCTA
GCACAAATGCTGCTTCAAGTGGTTCTTTGCCATCAATGTTTCCGGCTGGTAGTGCTCCTCCCCT
TTCTCCTAGAAGCACTTCTGGCTCCCCCCGTGTTATGAGGCGAGGATCTGGAGCTGGACCATCA
TCCTTGGGATCTCCACTAAAAGTAGTTAGTGAACCAGTGAGGGAGGTTATACCCCAGGTACTTC
TTATCTATTTTTGCAGTTAGTATTATTGTTGCGTTATTTCTGTTTGTCATCATTCGACCATATA
TTTATTTCTAATACTTCTTTGCATGCTATATGTTTTCAGTTTTACTTCAAATATGGGCGCCCTG
CTCCAAAAGATTTGAAGGAGCAATGCTTATCTAGAATAGATCACCTCTTTTTTGCAGGGGAGGG
ACTCCAGATTCAAGGTGATTGCTTTGAGGATTTCATTTCTTGATTTTACGCATTAAAAATACAT
TTCTAAGTTTGAATATTATATTTCTCCATATCAGAATTTAGATCAGTCACAAAAGATATATGCA
AACTTCCATCGTTCTTCTCCAGTGTTCTTTTCAAGAAGATCGATGCTGCAGGCTCTGGAACTGT
CACAAG

Sequences
Splice junction sequence	AATTTAGATCAGTCACAAAAGATATATGCAAACTTCCATCGTTCTTCTCCAGTGTTCTTTTCAA GAAGATCGATGCTGCAGGCTCTGGAACTGTCACAAGgtgaactctttgattgaagatgcaaaag ctggtgctacattgaatgttactgggagctctgctagcacaaatgctgcttcaagtggttcttt gccatcaa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 452 Transcript exons: 17758710-17758958,17759069-17759171,17759256-17759355 GTGAACTCTTTGATTGAAGATGCAAAAGCTGGTGCTACATTGAATGTTACTGGGAGCTCTGCTA GCACAAATGCTGCTTCAAGTGGTTCTTTGCCATCAATGTTTCCGGCTGGTAGTGCTCCTCCCCT TTCTCCTAGAAGCACTTCTGGCTCCCCCCGTGTTATGAGGCGAGGATCTGGAGCTGGACCATCA TCCTTGGGATCTCCACTAAAAGTAGTTAGTGAACCAGTGAGGGAGGTTATACCCCAGTTTTACT TCAAATATGGGCGCCCTGCTCCAAAAGATTTGAAGGAGCAATGCTTATCTAGAATAGATCACCT CTTTTTTGCAGGGGAGGGACTCCAGATTCAAGAATTTAGATCAGTCACAAAAGATATATGCAAA CTTCCATCGTTCTTCTCCAGTGTTCTTTTCAAGAAGATCGATGCTGCAGGCTCTGGAACTGTCA CAAG
Genomic sequence	GTGAACTCTTTGATTGAAGATGCAAAAGCTGGTGCTACATTGAATGTTACTGGGAGCTCTGCTA GCACAAATGCTGCTTCAAGTGGTTCTTTGCCATCAATGTTTCCGGCTGGTAGTGCTCCTCCCCT TTCTCCTAGAAGCACTTCTGGCTCCCCCCGTGTTATGAGGCGAGGATCTGGAGCTGGACCATCA TCCTTGGGATCTCCACTAAAAGTAGTTAGTGAACCAGTGAGGGAGGTTATACCCCAGGTACTTC TTATCTATTTTTGCAGTTAGTATTATTGTTGCGTTATTTCTGTTTGTCATCATTCGACCATATA TTTATTTCTAATACTTCTTTGCATGCTATATGTTTTCAGTTTTACTTCAAATATGGGCGCCCTG CTCCAAAAGATTTGAAGGAGCAATGCTTATCTAGAATAGATCACCTCTTTTTTGCAGGGGAGGG ACTCCAGATTCAAGGTGATTGCTTTGAGGATTTCATTTCTTGATTTTACGCATTAAAAATACAT TTCTAAGTTTGAATATTATATTTCTCCATATCAGAATTTAGATCAGTCACAAAAGATATATGCA AACTTCCATCGTTCTTCTCCAGTGTTCTTTTCAAGAAGATCGATGCTGCAGGCTCTGGAACTGT CACAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_008508 zma_circ_000816 						
					
						PMCS
						0.333509275

Conservation Information
Conserved circRNAs	osi_circ_008508 zma_circ_000816
PMCS	0.333509275

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							17758810-17758710(+)
17759282-17758710(+)
						
						Potential amino acid sequence
						
							MFPAGSAPPLSPRSTSGSPRVMRRGSGAGPSSLGSPLKVVSEPVREVIPQFYFKYGRPAPKDLK
EQCLSRIDHLFFAGEGLQIQEFRSVTKDICKLPSFFSSVLFKKIDAAGSGTVTR*(+)
MQTSIVLLQCSFQEDRCCRLWNCHKVNSLIEDAKAGATLNVTGSSASTNAASSGSLPSMFPAGS
APPLSPRSTSGSPRVMRRGSGAGPSSLGSPLKVVSEPVREVIPQFYFKYGRPAPKDLKEQCLSR
IDHLFFAGEGLQIQEFRSVTKDICKLPSFFSSVLFKKIDAAGSGTVTR*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	17758810-17758710(+) 17759282-17758710(+)
Potential amino acid sequence	MFPAGSAPPLSPRSTSGSPRVMRRGSGAGPSSLGSPLKVVSEPVREVIPQFYFKYGRPAPKDLK EQCLSRIDHLFFAGEGLQIQEFRSVTKDICKLPSFFSSVLFKKIDAAGSGTVTR(+) MQTSIVLLQCSFQEDRCCRLWNCHKVNSLIEDAKAGATLNVTGSSASTNAASSGSLPSMFPAGS APPLSPRSTSGSPRVMRRGSGAGPSSLGSPLKVVSEPVREVIPQFYFKYGRPAPKDLKEQCLSR IDHLFFAGEGLQIQEFRSVTKDICKLPSFFSSVLFKKIDAAGSGTVTR(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os10g0476600_circ_g.2

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