Plant Circular RNA Database

Detail information of Os06g0677400_circ_g.5

			
						 General Information
					
						CircRNA Name
						Os06g0677400_circ_g.5
					
						ID in PlantcircBase
						osa_circ_031996
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr6: 28140158-28141595  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os06g0677400						
					
						Parent gene annotation
						
							3-hydroxyisobutyrate dehydrogenase domain containing protein. (O
s06t0677400-01);Similar to 3-hydroxyisobutyrate dehydrogenase. (
Os06t0677400-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os06g0677400_circ_g.1 Os06g0677400_circ_g.2 Os06g0677400_circ_g.3 Os06g0677400_circ_g.4 						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os06t0677400-02:5
Os06t0677400-01:6

General Information
CircRNA Name	Os06g0677400_circ_g.5
ID in PlantcircBase	osa_circ_031996
Alias	NA
Organism	Oryza sativa
Position	chr6: 28140158-28141595 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer
Parent gene	Os06g0677400
Parent gene annotation	3-hydroxyisobutyrate dehydrogenase domain containing protein. (O s06t0677400-01);Similar to 3-hydroxyisobutyrate dehydrogenase. ( Os06t0677400-02)
Parent gene strand	+
Alternative splicing	Os06g0677400_circ_g.1 Os06g0677400_circ_g.2 Os06g0677400_circ_g.3 Os06g0677400_circ_g.4
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os06t0677400-02:5 Os06t0677400-01:6

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TATTGACTTAAACAGTGACACATATAACCCAGTTCCTGGAGTAATGATGGATGTGCCATCATCG
AGGAACTATGATGGTGGTTTCACCTCCAAACTAATGcaatgagaataccatgaagaaattctcg
gatgatggaattcccacaaaactgtctccacttgaagtgtccaagtctagtgatgtcgtaatta
cgatgctg

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							CAATGAGAATACCATGAAGAAATTCTCGGATGATGGAATTCCCACAAAACTGTCTCCACTTGAA
GTGTCCAAGTCTAGTGATGTCGTAATTACGATGCTGCCTTCGTCTGCCCATGTAAGCTGGTTGA
ACCATGATATCCCCTTCTTTTCAATTCCTTTTACACTCTTAGTCTCTTACTACCTAGTTAGATC
ATGATATCAGAGAGTAATACCCTCGCATGTCCCGCATGTAGAAATCTGTATACCGTAGCTTGTT
GCTGCATCGAACTTTTGCTCTAGAATTCTTAAAGAGATGGTGATGTATGAGTATTGTAGGTCAA
AACTTTAAAAAACCCCTTTAGAATATTAAAATCTTACGTGTTCACAGATGTTTTTCAGGTATTA
GATGTATACAATGGACGGAATGGTTTGCTTGCTAATGGGGGCTGCCTTGGACCTTGGTTGTATA
TAGATTCATCCACAGTTGATCCACAAACATCAAGGAAAATATCCATGGACATCTCAAGATGCAC
TTTGAAAGAAAAGAAAGGTTATATATTATAATAGCATATTAACCATGATTTGCTATTAGAAGTT
CAAAACTTGGATAGTTGAATATATTGTATGTGGGAAAGATGGAGCAAAAGCTTCTTCATCTTTT
AGCCCCGACAACAATTAAAACTGAAGTATATCTGAATATTTAAGGTGGTAATGTCACCTTTTGT
GTATTTCCAGCCTATGCTGAAAAACCGATGATGCTGGATGCTCCTGTTTCCGGAGGTGTTCCTG
CTGCAGAAGCTGGGAAACTCACTTTCATGGTATTCAATCCTTGCTTTTTCGTAACTTTGGTGTT
ATCACCAGGAATATACTTTGGTCGGACAGTTGCTACATTAGATGGAAGGATTTCTGTCTTATTT
TGGTGCAAAGGAATGATATATAGGTAGGTGGTTCAGAAGAAGCATATCTAGCAGCAAAACCCTT
ACTTCTCTCAATGGGCAAAAAAACAATATATTGTGGCGGGGCTGGAAATGGCTCGGTAAGTTTT
AGTAACTCTAGCTCTTCATTAAGATCTCTTCAAAACTGTTTGTGTACAGATGATTCAAGCTCAC
TTTTGATGCTCTATTTTTCAAGGTTGCAAAGATTTGTAACAACATGGCAATGGCCATCAGCATG
CTTGGAGTCTCCGAAGCTTTTGCTCTTGGTCAGAATCTCGGCATCAAAGCTAGCGTTCTTACAG
ACATATTCAACTGCTCAAGTGCCCGCTGCTGGAGCAGGTATTGTGTGCTCTTCACCTGTCCTTT
GTGGTTTGGGTTGCAGTTGACTTACAGGTTGAATAATCTGAAGTTTGCGTGCCCTTTCTATTGA
CTTAAACAGTGACACATATAACCCAGTTCCTGGAGTAATGATGGATGTGCCATCATCGAGGAAC
TATGATGGTGGTTTCACCTCCAAACTAATG

Sequences
Splice junction sequence	TATTGACTTAAACAGTGACACATATAACCCAGTTCCTGGAGTAATGATGGATGTGCCATCATCG AGGAACTATGATGGTGGTTTCACCTCCAAACTAATGcaatgagaataccatgaagaaattctcg gatgatggaattcccacaaaactgtctccacttgaagtgtccaagtctagtgatgtcgtaatta cgatgctg
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CAATGAGAATACCATGAAGAAATTCTCGGATGATGGAATTCCCACAAAACTGTCTCCACTTGAA GTGTCCAAGTCTAGTGATGTCGTAATTACGATGCTGCCTTCGTCTGCCCATGTAAGCTGGTTGA ACCATGATATCCCCTTCTTTTCAATTCCTTTTACACTCTTAGTCTCTTACTACCTAGTTAGATC ATGATATCAGAGAGTAATACCCTCGCATGTCCCGCATGTAGAAATCTGTATACCGTAGCTTGTT GCTGCATCGAACTTTTGCTCTAGAATTCTTAAAGAGATGGTGATGTATGAGTATTGTAGGTCAA AACTTTAAAAAACCCCTTTAGAATATTAAAATCTTACGTGTTCACAGATGTTTTTCAGGTATTA GATGTATACAATGGACGGAATGGTTTGCTTGCTAATGGGGGCTGCCTTGGACCTTGGTTGTATA TAGATTCATCCACAGTTGATCCACAAACATCAAGGAAAATATCCATGGACATCTCAAGATGCAC TTTGAAAGAAAAGAAAGGTTATATATTATAATAGCATATTAACCATGATTTGCTATTAGAAGTT CAAAACTTGGATAGTTGAATATATTGTATGTGGGAAAGATGGAGCAAAAGCTTCTTCATCTTTT AGCCCCGACAACAATTAAAACTGAAGTATATCTGAATATTTAAGGTGGTAATGTCACCTTTTGT GTATTTCCAGCCTATGCTGAAAAACCGATGATGCTGGATGCTCCTGTTTCCGGAGGTGTTCCTG CTGCAGAAGCTGGGAAACTCACTTTCATGGTATTCAATCCTTGCTTTTTCGTAACTTTGGTGTT ATCACCAGGAATATACTTTGGTCGGACAGTTGCTACATTAGATGGAAGGATTTCTGTCTTATTT TGGTGCAAAGGAATGATATATAGGTAGGTGGTTCAGAAGAAGCATATCTAGCAGCAAAACCCTT ACTTCTCTCAATGGGCAAAAAAACAATATATTGTGGCGGGGCTGGAAATGGCTCGGTAAGTTTT AGTAACTCTAGCTCTTCATTAAGATCTCTTCAAAACTGTTTGTGTACAGATGATTCAAGCTCAC TTTTGATGCTCTATTTTTCAAGGTTGCAAAGATTTGTAACAACATGGCAATGGCCATCAGCATG CTTGGAGTCTCCGAAGCTTTTGCTCTTGGTCAGAATCTCGGCATCAAAGCTAGCGTTCTTACAG ACATATTCAACTGCTCAAGTGCCCGCTGCTGGAGCAGGTATTGTGTGCTCTTCACCTGTCCTTT GTGGTTTGGGTTGCAGTTGACTTACAGGTTGAATAATCTGAAGTTTGCGTGCCCTTTCTATTGA CTTAAACAGTGACACATATAACCCAGTTCCTGGAGTAATGATGGATGTGCCATCATCGAGGAAC TATGATGGTGGTTTCACCTCCAAACTAATG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.158119431

Conservation Information
Conserved circRNAs	NA
PMCS	0.158119431

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							28140172-28140160(+)
28140274-28141581(-)
28140659-28141592(-)
						
						Potential amino acid sequence
						
							MKKFSDDGIPTKLSPLEVSKSSDVVITMLPSSAHVLDVYNGRNGLLANGGCLGPWLYIDSSTVD
PQTSRKISMDISRCTLKEKKAYAEKPMMLDAPVSGGVPAAEAGKLTFMVGGSEEAYLAAKPLLL
SMGKKTIYCGGAGNGSVAKICNNMAMAISMLGVSEAFALGQNLGIKASVLTDIFNCSSARCWSS
DTYNPVPGVMMDVPSSRNYDGGFTSKLMQ*(+)
MGRRRQHRNYDITRLGHFKWRQFCGNSIIREFLHGILIALVWR*(-)
MSMDIFLDVCGSTVDESIYNQGPRQPPLASKPFRPLYTSNTWADEGSIVITTSLDLDTSSGDSF
VGIPSSENFFMVFSLH*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	28140172-28140160(+) 28140274-28141581(-) 28140659-28141592(-)
Potential amino acid sequence	MKKFSDDGIPTKLSPLEVSKSSDVVITMLPSSAHVLDVYNGRNGLLANGGCLGPWLYIDSSTVD PQTSRKISMDISRCTLKEKKAYAEKPMMLDAPVSGGVPAAEAGKLTFMVGGSEEAYLAAKPLLL SMGKKTIYCGGAGNGSVAKICNNMAMAISMLGVSEAFALGQNLGIKASVLTDIFNCSSARCWSS DTYNPVPGVMMDVPSSRNYDGGFTSKLMQ(+) MGRRRQHRNYDITRLGHFKWRQFCGNSIIREFLHGILIALVWR(-) MSMDIFLDVCGSTVDESIYNQGPRQPPLASKPFRPLYTSNTWADEGSIVITTSLDLDTSSGDSF VGIPSSENFFMVFSLH*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os06g0677400_circ_g.5

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