Plant Circular RNA Database

Detail information of Os02g0255100_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os02g0255100_circ_g.3
					
						ID in PlantcircBase
						osa_circ_014131
					
						Alias
						Os_ciR4695
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 8763776-8765641  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os02g0255100						
					
						Parent gene annotation
						
							Mitochondrial protein phosphatase 2C protein 13, Pollen germinat
ion (Os02t0255100-01);Similar to Catalytic/ protein phosphatase 
type 2C/ protein serine/threonine phosphatase. (Os02t0255100-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os02g0255100_circ_g.1 Os02g0255100_circ_g.2 Os02g0255100_circ_g.4 Os02g0255100_circ_g.5 						
					
						Support reads
						4/1
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0255100-01:6
Os02t0255100-02:3

General Information
CircRNA Name	Os02g0255100_circ_g.3
ID in PlantcircBase	osa_circ_014131
Alias	Os_ciR4695
Organism	Oryza sativa
Position	chr2: 8763776-8765641 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os02g0255100
Parent gene annotation	Mitochondrial protein phosphatase 2C protein 13, Pollen germinat ion (Os02t0255100-01);Similar to Catalytic/ protein phosphatase type 2C/ protein serine/threonine phosphatase. (Os02t0255100-02)
Parent gene strand	+
Alternative splicing	Os02g0255100_circ_g.1 Os02g0255100_circ_g.2 Os02g0255100_circ_g.4 Os02g0255100_circ_g.5
Support reads	4/1
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0255100-01:6 Os02t0255100-02:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TGATTAATGTCTTAAGAAATTACAGGAAGAACTTGTCAATGAAGACTTGGAATGCCTCGTTCTT
GCTAGCGATGGACTTTGGGATGTCGTGGAAAATGAGagaagatggtaaactgaaatgtggatat
tcaagttttagagggaagagagccaccatggaggatttctatgatgtaaagttaactgaaatcg
atggacag

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							AGAAGATGGTAAACTGAAATGTGGATATTCAAGTTTTAGAGGGAAGAGAGCCACCATGGAGGAT
TTCTATGATGTAAAGTTAACTGAAATCGATGGACAGGCTGTTAGCCTATTTGGTGTATTTGATG
GTATGGTTTCCATCTTTCCTTGTCTACTTTCGTGATTCCGTGCCCACCATGTTTTTCTTTTGAA
GATTTGGTGCATTAGGTCATGGAGGACCACGTGCTGCTGAGTATTTGAAGGAAAATTTGTTCGA
AAATCTTCTGAAACACCCTGAGTTCCTGACAGATACAAAGCTTGCCATAAGTAATTTTTCTCTT
TGATTCTTACCTTTCCAGTTCTCTGTTCACCTTTTTTTTAAAGGTTGCTAATGATTATTATCTT
CTCAACTTTTGAAAAAAAAATCAAAACATAACAACAGGTGAAACATATCAGAAAACAGATACAG
ATTTCTTAGAATCAGAATCCAATGCCTTCAGGGATGATGGTTCAACAGCTTCCACTGCAGTTTT
GGTGGGTGGCCATCTATATGTAGCAAATGTTGGTGATTCCCGTGCTGTTGTTTCAAAAGCTGGT
AAAGGTATGTAACAACACATGCTTGTTGGATGTAGTTTTTCATCTGAACAACCACTTGGATTCT
TATCATTGATATCTGCTTAATGTTTCTAAATTTCCCAGCATTGATGTTTTTGTTGAAGAAGTTT
GGTACCTAATGTCTCATATAAACCCCCCTTGGCCCTTCTGCTAACGATTATTTCCTGTACCATA
ATTTAAGGCTAGTGACAAGGGTTGCTTATTTTCTAGACATTCTGGATCTGATATCTAGGTGGGA
TCTGAATTGGTAACTTAATTACTGAACTCTTGAAAGAACTAAGTTTACCTGCATGGTGCTGTTT
CTAATTTGCTTGGGCATGTTTGGTTTCCCTTCCCAACCTGGCTAGCCTTGGTTCACTCTACAAA
AGTGCACATGGGAGTCCGGTTTCAATACTTTCTAGTTTTTTTATTCAGTTTATATTTGTCTTCC
CCACCCATCCATTAACACCTAAAGTGCCCATGGGACTCCTGTTTCAATATTTTCTAGTTTTTTT
TATTCAGTTTATATTTGTCTTCCATACCCATCCATTAACACCTAAAGATTTTCACTTATTCGTT
TTTTCTAGCTATGGCACTGTCAGAAGATCACAAACCTAACAGGAGTGATGAGCGGAAGAGAATT
GAAAATGCAGGAGGAGTTGTCATTTGGGCTGGTAAGCTAAATCTTCATTCTTGGATGCACTGGG
CATAGTTTGCCTTTGTAAGCTCACATGGACAAGGCAGGTTGCACAAGGCTTGTCCATTTGGTGC
CTTCTGATCTCCTTGTAGTTTATGTTAGGTTCTCTATGTCATACCTGGGTTGTTTAGCATTGCA
GCCTTATTTACCTAAAATCATGCCATCTCTGTATATATTTTTCTGTTTGTTTTTTAAAAGTGAG
TTTATTCCCAGGTACTTGGAGGGTAGGAGGTGTACTGGCAATGTCGCGTGCATTTGGCAATCGT
CTACTGAAGCCATTTGTGGTGGCAGAGCCTGAAATTCAGGTATCTTATTGCACTTAATCATGCT
ATGATTTGTAGGCCTCATTTTATGTAGTTTAACCTACTCTAGTTAAAGGCTTAGCCTAGTCTAG
ATGAAAGTCAGAATAGGAGGTATTTGCAGTTCAACTTGTCTCTTTGTATGACTCCTCTGCTGTA
TAATTCTGATCATGACCATATAGCTTGTGTGTGAAGCTTGATTAATGTCTTAAGAAATTACAGG
AAGAACTTGTCAATGAAGACTTGGAATGCCTCGTTCTTGCTAGCGATGGACTTTGGGATGTCGT
GGAAAATGAG
						
					

			
		

Sequences
Splice junction sequence	TGATTAATGTCTTAAGAAATTACAGGAAGAACTTGTCAATGAAGACTTGGAATGCCTCGTTCTT GCTAGCGATGGACTTTGGGATGTCGTGGAAAATGAGagaagatggtaaactgaaatgtggatat tcaagttttagagggaagagagccaccatggaggatttctatgatgtaaagttaactgaaatcg atggacag
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	AGAAGATGGTAAACTGAAATGTGGATATTCAAGTTTTAGAGGGAAGAGAGCCACCATGGAGGAT TTCTATGATGTAAAGTTAACTGAAATCGATGGACAGGCTGTTAGCCTATTTGGTGTATTTGATG GTATGGTTTCCATCTTTCCTTGTCTACTTTCGTGATTCCGTGCCCACCATGTTTTTCTTTTGAA GATTTGGTGCATTAGGTCATGGAGGACCACGTGCTGCTGAGTATTTGAAGGAAAATTTGTTCGA AAATCTTCTGAAACACCCTGAGTTCCTGACAGATACAAAGCTTGCCATAAGTAATTTTTCTCTT TGATTCTTACCTTTCCAGTTCTCTGTTCACCTTTTTTTTAAAGGTTGCTAATGATTATTATCTT CTCAACTTTTGAAAAAAAAATCAAAACATAACAACAGGTGAAACATATCAGAAAACAGATACAG ATTTCTTAGAATCAGAATCCAATGCCTTCAGGGATGATGGTTCAACAGCTTCCACTGCAGTTTT GGTGGGTGGCCATCTATATGTAGCAAATGTTGGTGATTCCCGTGCTGTTGTTTCAAAAGCTGGT AAAGGTATGTAACAACACATGCTTGTTGGATGTAGTTTTTCATCTGAACAACCACTTGGATTCT TATCATTGATATCTGCTTAATGTTTCTAAATTTCCCAGCATTGATGTTTTTGTTGAAGAAGTTT GGTACCTAATGTCTCATATAAACCCCCCTTGGCCCTTCTGCTAACGATTATTTCCTGTACCATA ATTTAAGGCTAGTGACAAGGGTTGCTTATTTTCTAGACATTCTGGATCTGATATCTAGGTGGGA TCTGAATTGGTAACTTAATTACTGAACTCTTGAAAGAACTAAGTTTACCTGCATGGTGCTGTTT CTAATTTGCTTGGGCATGTTTGGTTTCCCTTCCCAACCTGGCTAGCCTTGGTTCACTCTACAAA AGTGCACATGGGAGTCCGGTTTCAATACTTTCTAGTTTTTTTATTCAGTTTATATTTGTCTTCC CCACCCATCCATTAACACCTAAAGTGCCCATGGGACTCCTGTTTCAATATTTTCTAGTTTTTTT TATTCAGTTTATATTTGTCTTCCATACCCATCCATTAACACCTAAAGATTTTCACTTATTCGTT TTTTCTAGCTATGGCACTGTCAGAAGATCACAAACCTAACAGGAGTGATGAGCGGAAGAGAATT GAAAATGCAGGAGGAGTTGTCATTTGGGCTGGTAAGCTAAATCTTCATTCTTGGATGCACTGGG CATAGTTTGCCTTTGTAAGCTCACATGGACAAGGCAGGTTGCACAAGGCTTGTCCATTTGGTGC CTTCTGATCTCCTTGTAGTTTATGTTAGGTTCTCTATGTCATACCTGGGTTGTTTAGCATTGCA GCCTTATTTACCTAAAATCATGCCATCTCTGTATATATTTTTCTGTTTGTTTTTTAAAAGTGAG TTTATTCCCAGGTACTTGGAGGGTAGGAGGTGTACTGGCAATGTCGCGTGCATTTGGCAATCGT CTACTGAAGCCATTTGTGGTGGCAGAGCCTGAAATTCAGGTATCTTATTGCACTTAATCATGCT ATGATTTGTAGGCCTCATTTTATGTAGTTTAACCTACTCTAGTTAAAGGCTTAGCCTAGTCTAG ATGAAAGTCAGAATAGGAGGTATTTGCAGTTCAACTTGTCTCTTTGTATGACTCCTCTGCTGTA TAATTCTGATCATGACCATATAGCTTGTGTGTGAAGCTTGATTAATGTCTTAAGAAATTACAGG AAGAACTTGTCAATGAAGACTTGGAATGCCTCGTTCTTGCTAGCGATGGACTTTGGGATGTCGT GGAAAATGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.16158549

Conservation Information
Conserved circRNAs	NA
PMCS	0.16158549

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							8763832-8763784(+)
8765581-8763784(+)
8763834-8765314(-)
						
						Potential amino acid sequence
						
							MEDFYDVKLTEIDGQAVSLFGVFDGHGGPRAAEYLKENLFENLLKHPEFLTDTKLAISETYQKT
DTDFLESESNAFRDDGSTASTAVLVGGHLYVANVGDSRAVVSKAGKAMALSEDHKPNRSDERKR
IENAGGVVIWAGTWRVGGVLAMSRAFGNRLLKPFVVAEPEIQEELVNEDLECLVLASDGLWDVV
ENERRW*(+)
MKTWNASFLLAMDFGMSWKMREDGKLKCGYSSFRGKRATMEDFYDVKLTEIDGQAVSLFGVFDG
HGGPRAAEYLKENLFENLLKHPEFLTDTKLAISETYQKTDTDFLESESNAFRDDGSTASTAVLV
GGHLYVANVGDSRAVVSKAGKAMALSEDHKPNRSDERKRIENAGGVVIWAGTWRVGGVLAMSRA
FGNRLLKPFVVAEPEIQEELVNEDLECLVLASDGLWDVVENERRW*(+)
MVALFPLKLEYPHFSLPSSLIFHDIPKSIASKNEAFQVFIDKFFLNFRLCHHKWLQ*(-)
						
						Sponge-miRNAs
						
							osa-miR2093-5p 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	8763832-8763784(+) 8765581-8763784(+) 8763834-8765314(-)
Potential amino acid sequence	MEDFYDVKLTEIDGQAVSLFGVFDGHGGPRAAEYLKENLFENLLKHPEFLTDTKLAISETYQKT DTDFLESESNAFRDDGSTASTAVLVGGHLYVANVGDSRAVVSKAGKAMALSEDHKPNRSDERKR IENAGGVVIWAGTWRVGGVLAMSRAFGNRLLKPFVVAEPEIQEELVNEDLECLVLASDGLWDVV ENERRW(+) MKTWNASFLLAMDFGMSWKMREDGKLKCGYSSFRGKRATMEDFYDVKLTEIDGQAVSLFGVFDG HGGPRAAEYLKENLFENLLKHPEFLTDTKLAISETYQKTDTDFLESESNAFRDDGSTASTAVLV GGHLYVANVGDSRAVVSKAGKAMALSEDHKPNRSDERKRIENAGGVVIWAGTWRVGGVLAMSRA FGNRLLKPFVVAEPEIQEELVNEDLECLVLASDGLWDVVENERRW(+) MVALFPLKLEYPHFSLPSSLIFHDIPKSIASKNEAFQVFIDKFFLNFRLCHHKWLQ*(-)
Sponge-miRNAs	osa-miR2093-5p
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os02g0255100_circ_g.3

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