Plant Circular RNA Database

Detail information of Zm00001d025461_circ_g.2

			
						 General Information
					
						CircRNA Name
						Zm00001d025461_circ_g.2
					
						ID in PlantcircBase
						zma_circ_010311
					
						Alias
						Zm10circ00054, GRMZM2G139419_C1
					
						Organism
						
							Zea mays						
					
						Position
						
							chr10: 119224024-119225286  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRI2
					
						Parent gene
						
							Zm00001d025461						
					
						Parent gene annotation
						
							Regulator of nonsense transcripts UPF3
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Zm00001d025461_circ_g.1 Zm00001d025461_circ_g.3 Zm00001d025461_circ_g.4 						
					
						Support reads
						NA
					
						Tissues
						leaf, endosperm
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d025461_T002:5
Zm00001d025461_T015:4
Zm00001d025461_T010:5
Zm00001d025461_T009:5
Zm00001d025461_T007:4
Zm00001d025461_T014:5
Zm00001d025461_T005:5
Zm00001d025461_T016:3
Zm00001d025461_T012:5
Zm00001d025461_T013:5
Zm00001d025461_T004:5
Zm00001d025461_T001:5
Zm00001d025461_T008:5
Zm00001d025461_T017:5
Zm00001d025461_T011:5
Zm00001d025461_T003:5
Zm00001d025461_T006:5

General Information
CircRNA Name	Zm00001d025461_circ_g.2
ID in PlantcircBase	zma_circ_010311
Alias	Zm10circ00054, GRMZM2G139419_C1
Organism	Zea mays
Position	chr10: 119224024-119225286 JBrowse»
Reference genome	AGPv4.38
Type	u-circRNA
Identification method	CIRI2
Parent gene	Zm00001d025461
Parent gene annotation	Regulator of nonsense transcripts UPF3
Parent gene strand	-
Alternative splicing	Zm00001d025461_circ_g.1 Zm00001d025461_circ_g.3 Zm00001d025461_circ_g.4
Support reads	NA
Tissues	leaf, endosperm
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d025461_T002:5 Zm00001d025461_T015:4 Zm00001d025461_T010:5 Zm00001d025461_T009:5 Zm00001d025461_T007:4 Zm00001d025461_T014:5 Zm00001d025461_T005:5 Zm00001d025461_T016:3 Zm00001d025461_T012:5 Zm00001d025461_T013:5 Zm00001d025461_T004:5 Zm00001d025461_T001:5 Zm00001d025461_T008:5 Zm00001d025461_T017:5 Zm00001d025461_T011:5 Zm00001d025461_T003:5 Zm00001d025461_T006:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ACATTAGAATCAATCCTTCCTCTTCCTTTCAATATGACAATTTTATCCCTTTTAAACTCAGCAA
CCTGACCAGATCCAGATGTTCCCCCACTTGTGGCATcatcaatgaacaaactcacgagcctgaa
cttgacttctgaacccatacttgtctctgcacaaggcattcatgatattaaatttggatgaaaa
attttgaa

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							CATCAATGAACAAACTCACGAGCCTGAACTTGACTTCTGAACCCATACTTGTCTCTGCACAAGG
CATTCATGATATTAAATTTGGATGAAAAATTTTGAACATAAATCAAAGACCCCTGCAATCAGCG
ATGGGACATGAAATAAAATTGTACTCGACCAACGTAGGATGAACTAAGTGGCTTCTTAATTAAT
AAATACAGACCTAAATTCTCCTCAATGAGGACTTGGACATGGGTGGTCTGAGTGTGCACCCACA
ACCTTGACCAACCAGCAAATAAGAAATTAAAAAATAACCTCACCTCATGAGCACCATAGCTAGC
AGAGCCCCTCTTTGAATTCTTCGCATCAGGCACACTTGCAGATATTTCAGTTTCCTTTGGTCCA
CGTGGAAGGCCACGACGGTTGGCGTGCCTATGTCCTGGATTAAAAACTGTGTCATGAAACTGAT
CTAGAAAAGCTACACATCTTATTTTTGCATACAAACCATTTTCAACAGGAACACCACGGCTACC
ACCTTGACCCCTTGTGTCTGTCTTTCTGTCTCCATGACCTGTGTTGAGTTCAAGTTTTGAGACT
GCAGGCATCACCAATGCTGCAACTATTTCAGCAAATTACATACCATCTGCTAGTTGCAAAATAT
TATCTCCAGAATGTGACTGCATTTGAGGGAACTCTGACGACGAAGGATGTGAACTACCTGCAGA
TGTAGATTTGTCATAGCGGCGAGCTGCCCATACACCACGATCGGGTCTATCTCTGTTTCTAGCA
TGCCTTTCAACCTTCTCACCAATGGGAACGGAGCCATGGAGATGATTAGCCTTCTCATCAAAAT
GGCTCCTCTCAGCATTTTCAACTACCTGATCTTTCACAATGGTACGAGGGTTGAAAGCCCGTGG
TGGCCGCTTATCCTTCTCAGTATTAAACATGTGACCTTCCTGCTCATGTTCAGAAGGATTTGAA
CTGCGGGCTTCCTTGTTTGATAGTATAGTCTTAATAATTTTTCCACTGGCTTCAAGTCGCTGGT
CTTGTCTAGAACGCGATGGTGGCACATTCTTGATAGGGGTTGATGAGTGCTGAGTTGCTCCATT
AGGTGTCTGTGGTAAAAATTGATCAAACATAGGTCCATAATAATAAAAGTTATAAAACTGCAAA
AATGTTAACTTACATTAGAATCAATCCTTCCTCTTCCTTTCAATATGACAATTTTATCCCTTTT
AAACTCAGCAACCTGACCAGATCCAGATGTTCCCCCACTTGTGGCAT

Sequences
Splice junction sequence	ACATTAGAATCAATCCTTCCTCTTCCTTTCAATATGACAATTTTATCCCTTTTAAACTCAGCAA CCTGACCAGATCCAGATGTTCCCCCACTTGTGGCATcatcaatgaacaaactcacgagcctgaa cttgacttctgaacccatacttgtctctgcacaaggcattcatgatattaaatttggatgaaaa attttgaa
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CATCAATGAACAAACTCACGAGCCTGAACTTGACTTCTGAACCCATACTTGTCTCTGCACAAGG CATTCATGATATTAAATTTGGATGAAAAATTTTGAACATAAATCAAAGACCCCTGCAATCAGCG ATGGGACATGAAATAAAATTGTACTCGACCAACGTAGGATGAACTAAGTGGCTTCTTAATTAAT AAATACAGACCTAAATTCTCCTCAATGAGGACTTGGACATGGGTGGTCTGAGTGTGCACCCACA ACCTTGACCAACCAGCAAATAAGAAATTAAAAAATAACCTCACCTCATGAGCACCATAGCTAGC AGAGCCCCTCTTTGAATTCTTCGCATCAGGCACACTTGCAGATATTTCAGTTTCCTTTGGTCCA CGTGGAAGGCCACGACGGTTGGCGTGCCTATGTCCTGGATTAAAAACTGTGTCATGAAACTGAT CTAGAAAAGCTACACATCTTATTTTTGCATACAAACCATTTTCAACAGGAACACCACGGCTACC ACCTTGACCCCTTGTGTCTGTCTTTCTGTCTCCATGACCTGTGTTGAGTTCAAGTTTTGAGACT GCAGGCATCACCAATGCTGCAACTATTTCAGCAAATTACATACCATCTGCTAGTTGCAAAATAT TATCTCCAGAATGTGACTGCATTTGAGGGAACTCTGACGACGAAGGATGTGAACTACCTGCAGA TGTAGATTTGTCATAGCGGCGAGCTGCCCATACACCACGATCGGGTCTATCTCTGTTTCTAGCA TGCCTTTCAACCTTCTCACCAATGGGAACGGAGCCATGGAGATGATTAGCCTTCTCATCAAAAT GGCTCCTCTCAGCATTTTCAACTACCTGATCTTTCACAATGGTACGAGGGTTGAAAGCCCGTGG TGGCCGCTTATCCTTCTCAGTATTAAACATGTGACCTTCCTGCTCATGTTCAGAAGGATTTGAA CTGCGGGCTTCCTTGTTTGATAGTATAGTCTTAATAATTTTTCCACTGGCTTCAAGTCGCTGGT CTTGTCTAGAACGCGATGGTGGCACATTCTTGATAGGGGTTGATGAGTGCTGAGTTGCTCCATT AGGTGTCTGTGGTAAAAATTGATCAAACATAGGTCCATAATAATAAAAGTTATAAAACTGCAAA AATGTTAACTTACATTAGAATCAATCCTTCCTCTTCCTTTCAATATGACAATTTTATCCCTTTT AAACTCAGCAACCTGACCAGATCCAGATGTTCCCCCACTTGTGGCAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.143702745

Conservation Information
Conserved circRNAs	NA
PMCS	0.143702745

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							119224071-119224041(-)
119224028-119225217(-)
						
						Potential amino acid sequence
						
							MGSEVKFRLVSLFIDDATSGGTSGSGQVAEFKRDKIVILKGRGRIDSNTPNGATQHSSTPIKNV
PPSRSRQDQRLEASGKIIKTILSNKEARSSNPSEHEQEGHMFNTEKDKRPPRAFNPRTIVKDQV
VENAERSHFDEKANHLHGSVPIGEKVERHARNRDRPDRGVWAARRYDKSTSAGSSHPSSSEFPQ
MQSHSGDNILQLADGHGDRKTDTRGQGGSRGVPVENGHRHANRRGLPRGPKETEISASVPDAKN
SKRGSASYGAHERQVWVQKSSSGS*(-)
MMPQVGEHLDLVRLLSLKGIKLSY*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to drought stress

Functional Information
Coding potential	Y
Potential coding position	119224071-119224041(-) 119224028-119225217(-)
Potential amino acid sequence	MGSEVKFRLVSLFIDDATSGGTSGSGQVAEFKRDKIVILKGRGRIDSNTPNGATQHSSTPIKNV PPSRSRQDQRLEASGKIIKTILSNKEARSSNPSEHEQEGHMFNTEKDKRPPRAFNPRTIVKDQV VENAERSHFDEKANHLHGSVPIGEKVERHARNRDRPDRGVWAARRYDKSTSAGSSHPSSSEFPQ MQSHSGDNILQLADGHGDRKTDTRGQGGSRGVPVENGHRHANRRGLPRGPKETEISASVPDAKN SKRGSASYGAHERQVWVQKSSSGS(-) MMPQVGEHLDLVRLLSLKGIKLSY(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to drought stress

			
						 Other Information
					
						References
						Han et al., 2020;Zhang et al., 2019

circRNA

Detail information of Zm00001d025461_circ_g.2

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