Plant Circular RNA Database

Detail information of Os06g0209300_circ_g.5

			
						 General Information
					
						CircRNA Name
						Os06g0209300_circ_g.5
					
						ID in PlantcircBase
						osa_circ_030159
					
						Alias
						Os_ciR10746
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr6: 5578417-5578635  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os06g0209300						
					
						Parent gene annotation
						
							Hypothetical conserved gene. (Os06t0209300-01);Conserved hypothe
tical protein. (Os06t0209300-02);Hypothetical conserved gene. (O
s06t0209300-03)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os06g0209300_circ_g.2 Os06g0209300_circ_g.3 Os06g0209300_circ_g.4 						
					
						Support reads
						2/2
					
						Tissues
						root/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os06t0209300-02:1

General Information
CircRNA Name	Os06g0209300_circ_g.5
ID in PlantcircBase	osa_circ_030159
Alias	Os_ciR10746
Organism	Oryza sativa
Position	chr6: 5578417-5578635 JBrowse»
Reference genome	IRGSP-1.0.38
Type	u-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os06g0209300
Parent gene annotation	Hypothetical conserved gene. (Os06t0209300-01);Conserved hypothe tical protein. (Os06t0209300-02);Hypothetical conserved gene. (O s06t0209300-03)
Parent gene strand	-
Alternative splicing	Os06g0209300_circ_g.2 Os06g0209300_circ_g.3 Os06g0209300_circ_g.4
Support reads	2/2
Tissues	root/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os06t0209300-02:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TGCCAGCGACAACATCTGATGGCATCTCTTGGCAAAGTTCACACTTCCAGGGACCTGTGGGATT
CGTCACACTCCGGTAGCAATCCAAATGCACAGCAGCctcagcacaaaaggcatgaatacatcgc
ccctttattgtttttctaaaagcaccagatgttccatggcacaaatcgcactgcaacaagcatg
gttttgaa

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 207
Transcript exons: 5578417-5578548,5578561-5578635
CTCAGCACAAAAGGCATGAATACATCGCCCCTTTATTGTTTTTCTAAAAGCACCAGATGTTCCA
TGGCACAAATCGCACTGCAACAAGCATGGTTTTGAACCGTCGCAATCTGATTGACTGCCAGCGA
CAACCTCTTGGCAAAGTTCACACTTCCAGGGACCTGTGGGATTCGTCACACTCCGGTAGCAATC
CAAATGCACAGCAGC
Transcript length: 219
Transcript exons: 5578417-5578635
CTCAGCACAAAAGGCATGAATACATCGCCCCTTTATTGTTTTTCTAAAAGCACCAGATGTTCCA
TGGCACAAATCGCACTGCAACAAGCATGGTTTTGAACCGTCGCAATCTGATTGACTGCCAGCGA
CAACATCTGATGGCATCTCTTGGCAAAGTTCACACTTCCAGGGACCTGTGGGATTCGTCACACT
CCGGTAGCAATCCAAATGCACAGCAGC
						
						Genomic sequence
						
							CTCAGCACAAAAGGCATGAATACATCGCCCCTTTATTGTTTTTCTAAAAGCACCAGATGTTCCA
TGGCACAAATCGCACTGCAACAAGCATGGTTTTGAACCGTCGCAATCTGATTGACTGCCAGCGA
CAACATCTGATGGCATCTCTTGGCAAAGTTCACACTTCCAGGGACCTGTGGGATTCGTCACACT
CCGGTAGCAATCCAAATGCACAGCAGC

Sequences
Splice junction sequence	TGCCAGCGACAACATCTGATGGCATCTCTTGGCAAAGTTCACACTTCCAGGGACCTGTGGGATT CGTCACACTCCGGTAGCAATCCAAATGCACAGCAGCctcagcacaaaaggcatgaatacatcgc ccctttattgtttttctaaaagcaccagatgttccatggcacaaatcgcactgcaacaagcatg gttttgaa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 207 Transcript exons: 5578417-5578548,5578561-5578635 CTCAGCACAAAAGGCATGAATACATCGCCCCTTTATTGTTTTTCTAAAAGCACCAGATGTTCCA TGGCACAAATCGCACTGCAACAAGCATGGTTTTGAACCGTCGCAATCTGATTGACTGCCAGCGA CAACCTCTTGGCAAAGTTCACACTTCCAGGGACCTGTGGGATTCGTCACACTCCGGTAGCAATC CAAATGCACAGCAGC Transcript length: 219 Transcript exons: 5578417-5578635 CTCAGCACAAAAGGCATGAATACATCGCCCCTTTATTGTTTTTCTAAAAGCACCAGATGTTCCA TGGCACAAATCGCACTGCAACAAGCATGGTTTTGAACCGTCGCAATCTGATTGACTGCCAGCGA CAACATCTGATGGCATCTCTTGGCAAAGTTCACACTTCCAGGGACCTGTGGGATTCGTCACACT CCGGTAGCAATCCAAATGCACAGCAGC
Genomic sequence	CTCAGCACAAAAGGCATGAATACATCGCCCCTTTATTGTTTTTCTAAAAGCACCAGATGTTCCA TGGCACAAATCGCACTGCAACAAGCATGGTTTTGAACCGTCGCAATCTGATTGACTGCCAGCGA CAACATCTGATGGCATCTCTTGGCAAAGTTCACACTTCCAGGGACCTGTGGGATTCGTCACACT CCGGTAGCAATCCAAATGCACAGCAGC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.326716971

Conservation Information
Conserved circRNAs	NA
PMCS	0.326716971

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							5578625-5578512(+)
5578474-5578460(+)
5578562-5578419(-)
						
						Potential amino acid sequence
						
							MHSSLSTKGMNTSPLYCFSKSTRCSMAQIALQQAWF*(+)
MFHGTNRTATSMVLNRRNLIDCQRQHLMASLGKVHTSRDLWDSSHSGSNPNAQQPQHKRHEYIA
PLLFF*(+)
MPSDVVAGSQSDCDGSKPCLLQCDLCHGTSGAFRKTIKGRCIHAFCAEAAVHLDCYRSVTNPTG
PWKCELCQEMPSDVVAGSQSDCDGSKPCLLQCDLCHGTSGAFRKTIKGRCIHAFCAEAAVHLDC
YRSVTNPTGPWKCELCQEMPSDVVAGSQSDCDGSKPCLLQCDLCHGTSGAFRKTIKGRCIHAFC
AEAAVHLDCYRSVTNPTGPWKCELCQEMPSDVVAGSQSDCDGSKPCLLQCDLCHGTSGAFRKTI
KGRCIHAFCAE(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	5578625-5578512(+) 5578474-5578460(+) 5578562-5578419(-)
Potential amino acid sequence	MHSSLSTKGMNTSPLYCFSKSTRCSMAQIALQQAWF(+) MFHGTNRTATSMVLNRRNLIDCQRQHLMASLGKVHTSRDLWDSSHSGSNPNAQQPQHKRHEYIA PLLFF(+) MPSDVVAGSQSDCDGSKPCLLQCDLCHGTSGAFRKTIKGRCIHAFCAEAAVHLDCYRSVTNPTG PWKCELCQEMPSDVVAGSQSDCDGSKPCLLQCDLCHGTSGAFRKTIKGRCIHAFCAEAAVHLDC YRSVTNPTGPWKCELCQEMPSDVVAGSQSDCDGSKPCLLQCDLCHGTSGAFRKTIKGRCIHAFC AEAAVHLDCYRSVTNPTGPWKCELCQEMPSDVVAGSQSDCDGSKPCLLQCDLCHGTSGAFRKTI KGRCIHAFCAE(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os06g0209300_circ_g.5

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