Plant Circular RNA Database

Detail information of Os12g0527800_circ_g.7

			
						 General Information
					
						CircRNA Name
						Os12g0527800_circ_g.7
					
						ID in PlantcircBase
						osa_circ_011842
					
						Alias
						Os12circ07891/Os_ciR2664
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr12: 20810612-20811713  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer, SMALT, Segemehl, find_circ
					
						Parent gene
						
							Os12g0527800						
					
						Parent gene annotation
						
							Zinc finger, RING/FYVE/PHD-type domain containing protein. (Os12
t0527800-01);Hypothetical conserved gene. (Os12t0527800-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os12g0527800_circ_g.4 Os12g0527800_circ_g.5 Os12g0527800_circ_g.6 						
					
						Support reads
						3/5/1
					
						Tissues
						leaf/root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os12t0527800-01:3

General Information
CircRNA Name	Os12g0527800_circ_g.7
ID in PlantcircBase	osa_circ_011842
Alias	Os12circ07891/Os_ciR2664
Organism	Oryza sativa
Position	chr12: 20810612-20811713 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer, SMALT, Segemehl, find_circ
Parent gene	Os12g0527800
Parent gene annotation	Zinc finger, RING/FYVE/PHD-type domain containing protein. (Os12 t0527800-01);Hypothetical conserved gene. (Os12t0527800-02)
Parent gene strand	-
Alternative splicing	Os12g0527800_circ_g.4 Os12g0527800_circ_g.5 Os12g0527800_circ_g.6
Support reads	3/5/1
Tissues	leaf/root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os12t0527800-01:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TACTACCCTCATTTTCTCCCGAAAAGCATATACGACACATAACCTTGACAGCAGCATTGCCCAC
ATCTTCTTGGCCCCAGTCCTCTTCATCTCCTTCCTCcttgtgcgacggttgctgacagtagcag
tgataagcaccatcacaccttttacagaacattaacttgttgggatcaccaggtcgtcgacata
cctgtacg

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 327
Transcript exons: 20810612-20810704,20811401-20811450,20811530-20811713
CTTGTGCGACGGTTGCTGACAGTAGCAGTGATAAGCACCATCACACCTTTTACAGAACATTAAC
TTGTTGGGATCACCAGGTCGTCGACATACCTCACAACTGCGGCAAGAGGGGCAGACCCATGAGC
TCCAATGGAAAAGATCTCTATGTTCCCCCCAGTTTTTCAAACAACTTCTATGATACTTTTTGTT
GCAGAGTTTGCATGGAAGCATTTTGGCAGCTTTTGTACTACCCTCATTTTCTCCCGAAAAGCAT
ATACGACACATAACCTTGACAGCAGCATTGCCCACATCTTCTTGGCCCCAGTCCTCTTCATCTC
CTTCCTC
						
						Genomic sequence
						
							CTTGTGCGACGGTTGCTGACAGTAGCAGTGATAAGCACCATCACACCTTTTACAGAACATTAAC
TTGTTGGGATCACCAGGTCGTCGACATACCTGTACGTATGACAAGTAGTTATGGTATAAAAACA
TTTGAGATGCTAGTGTAGAATTCAGTGTTATTAGACCTGGACAAGCCCAGCTGTTCCAGGCACC
TGAGCCAGGATGGTCCAGGCAGCAAGACCAGTTAAGCAATTGACCCGGCAAAATTCCAGTTCAA
CCAGCAGTTTTTCAATCCATGGTGGACATGTGGCTCCATTTTTCAAAAATGGAATGCAATGCTG
GGACTCGAATCCCAGATCTCCCACCCATCCATTCATGGTTCAAATGGTGAACTATGTATGATAT
AGGATGATAAATATTGTATGGCACAAGCGGTCCAACCGATGACCCAGAGATTGGACCAGTGACC
CCGCAAACCAACGTCTTGGCCAGATTGACATCAAATCCAAGACTCCAGTCTAGTAACACTGGTA
GAATTATGGCATGAAAAACAAATGATAATCTACATGAAGAAATGGGGGGAGTGCACTTCGACAT
TAACATGAATGCATGGCCACCATAACAGCTGGTACGTGTATAGGCACTAGATCCCTCAAGTACA
GTAAATGGCCTAACTTTGCAGCCCTTTAACTTGAAGAGGTGAAGTTTCCACAGGAAACAAAGCT
TAACTTTACTGGATAAGGTACTTCTGGAGGCAAAGAAAAAAAATACAAAGGTGTGAAACACAAT
TTAAATATACAGACAACTAACCTCACAACTGCGGCAAGAGGGGCAGACCCATGAGCTCCAATGG
AAAAGATCTAGATGGAACATAAACTTGTCAAAGGCTTATAAAATAAAGTATTCAAAGAGGTAGT
AAAATGAGACATTTTTTATTACCTCTATGTTCCCCCCAGTTTTTCAAACAACTTCTATGATACT
TTTTGTTGCAGAGTTTGCATGGAAGCATTTTGGCAGCTTTTGTACTACCCTCATTTTCTCCCGA
AAAGCATATACGACACATAACCTTGACAGCAGCATTGCCCACATCTTCTTGGCCCCAGTCCTCT
TCATCTCCTTCCTC

Sequences
Splice junction sequence	TACTACCCTCATTTTCTCCCGAAAAGCATATACGACACATAACCTTGACAGCAGCATTGCCCAC ATCTTCTTGGCCCCAGTCCTCTTCATCTCCTTCCTCcttgtgcgacggttgctgacagtagcag tgataagcaccatcacaccttttacagaacattaacttgttgggatcaccaggtcgtcgacata cctgtacg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 327 Transcript exons: 20810612-20810704,20811401-20811450,20811530-20811713 CTTGTGCGACGGTTGCTGACAGTAGCAGTGATAAGCACCATCACACCTTTTACAGAACATTAAC TTGTTGGGATCACCAGGTCGTCGACATACCTCACAACTGCGGCAAGAGGGGCAGACCCATGAGC TCCAATGGAAAAGATCTCTATGTTCCCCCCAGTTTTTCAAACAACTTCTATGATACTTTTTGTT GCAGAGTTTGCATGGAAGCATTTTGGCAGCTTTTGTACTACCCTCATTTTCTCCCGAAAAGCAT ATACGACACATAACCTTGACAGCAGCATTGCCCACATCTTCTTGGCCCCAGTCCTCTTCATCTC CTTCCTC
Genomic sequence	CTTGTGCGACGGTTGCTGACAGTAGCAGTGATAAGCACCATCACACCTTTTACAGAACATTAAC TTGTTGGGATCACCAGGTCGTCGACATACCTGTACGTATGACAAGTAGTTATGGTATAAAAACA TTTGAGATGCTAGTGTAGAATTCAGTGTTATTAGACCTGGACAAGCCCAGCTGTTCCAGGCACC TGAGCCAGGATGGTCCAGGCAGCAAGACCAGTTAAGCAATTGACCCGGCAAAATTCCAGTTCAA CCAGCAGTTTTTCAATCCATGGTGGACATGTGGCTCCATTTTTCAAAAATGGAATGCAATGCTG GGACTCGAATCCCAGATCTCCCACCCATCCATTCATGGTTCAAATGGTGAACTATGTATGATAT AGGATGATAAATATTGTATGGCACAAGCGGTCCAACCGATGACCCAGAGATTGGACCAGTGACC CCGCAAACCAACGTCTTGGCCAGATTGACATCAAATCCAAGACTCCAGTCTAGTAACACTGGTA GAATTATGGCATGAAAAACAAATGATAATCTACATGAAGAAATGGGGGGAGTGCACTTCGACAT TAACATGAATGCATGGCCACCATAACAGCTGGTACGTGTATAGGCACTAGATCCCTCAAGTACA GTAAATGGCCTAACTTTGCAGCCCTTTAACTTGAAGAGGTGAAGTTTCCACAGGAAACAAAGCT TAACTTTACTGGATAAGGTACTTCTGGAGGCAAAGAAAAAAAATACAAAGGTGTGAAACACAAT TTAAATATACAGACAACTAACCTCACAACTGCGGCAAGAGGGGCAGACCCATGAGCTCCAATGG AAAAGATCTAGATGGAACATAAACTTGTCAAAGGCTTATAAAATAAAGTATTCAAAGAGGTAGT AAAATGAGACATTTTTTATTACCTCTATGTTCCCCCCAGTTTTTCAAACAACTTCTATGATACT TTTTGTTGCAGAGTTTGCATGGAAGCATTTTGGCAGCTTTTGTACTACCCTCATTTTCTCCCGA AAAGCATATACGACACATAACCTTGACAGCAGCATTGCCCACATCTTCTTGGCCCCAGTCCTCT TCATCTCCTTCCTC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_003462* osi_circ_009760 						
					
						PMCS
						0.2502094

Conservation Information
Conserved circRNAs	osi_circ_003462* osi_circ_009760
PMCS	0.2502094

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							20811441-20810671(+)
20811655-20810614(-)
20810654-20811553(-)
						
						Potential amino acid sequence
						
							MEKISMFPPVFQTTSMILFVAEFAWKHFGSFCTTLIFSRKAYTTHNLDSSIAHIFLAPVLFISF
LLVRRLLTVAVISTITPFTEH*(+)
MCRICFSGENEGSTKAAKMLPCKLCNKKYHRSCLKNWGEHRDLFHWSSWVCPSCRSCEVCRRPG
DPNKLMFCKRCDGAYHCYCQQPSHKEEGDEEDWGQEDVGNAAVKVMCRICFSGENEGSTKAAKM
LPCKLCNKKYHRSCLKNWGEHRDLFHWSSWVCPSCRSCEVCRRPGDPNKLMFCKRCDGAYHCYC
QQPSHKEEGDEEDWGQEDVGNAAVKVMCRICFSGENEGSTKAAKMLPCKLCNKKYHRSCLKNWG
EHRDLFHWSSWVCPSCRSCEVCRRPGDPNKLMFCKRCDGAYHCYCQQPSHKEEGDEEDWGQEDV
GNAAVKVMCRICFSGENEGSTKAAKMLPCKLCNKKYHRSCLKNWGEHRDLFHWSSWVCPSCRSC
EVCRRPGDPNKLMFCKRCDGAYHCYCQQPSHK(-)
MVLITATVSNRRTRRKEMKRTGAKKMWAMLLSRLCVVYAFREKMRVVQKLPKCFHANSATKSII
EVV*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	20811441-20810671(+) 20811655-20810614(-) 20810654-20811553(-)
Potential amino acid sequence	MEKISMFPPVFQTTSMILFVAEFAWKHFGSFCTTLIFSRKAYTTHNLDSSIAHIFLAPVLFISF LLVRRLLTVAVISTITPFTEH(+) MCRICFSGENEGSTKAAKMLPCKLCNKKYHRSCLKNWGEHRDLFHWSSWVCPSCRSCEVCRRPG DPNKLMFCKRCDGAYHCYCQQPSHKEEGDEEDWGQEDVGNAAVKVMCRICFSGENEGSTKAAKM LPCKLCNKKYHRSCLKNWGEHRDLFHWSSWVCPSCRSCEVCRRPGDPNKLMFCKRCDGAYHCYC QQPSHKEEGDEEDWGQEDVGNAAVKVMCRICFSGENEGSTKAAKMLPCKLCNKKYHRSCLKNWG EHRDLFHWSSWVCPSCRSCEVCRRPGDPNKLMFCKRCDGAYHCYCQQPSHKEEGDEEDWGQEDV GNAAVKVMCRICFSGENEGSTKAAKMLPCKLCNKKYHRSCLKNWGEHRDLFHWSSWVCPSCRSC EVCRRPGDPNKLMFCKRCDGAYHCYCQQPSHK(-) MVLITATVSNRRTRRKEMKRTGAKKMWAMLLSRLCVVYAFREKMRVVQKLPKCFHANSATKSII EVV(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015;Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os12g0527800_circ_g.7

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