Plant Circular RNA Database

Detail information of Csa4G008220_circ_g.4

			
						 General Information
					
						CircRNA Name
						Csa4G008220_circ_g.4
					
						ID in PlantcircBase
						csa_circ_002288
					
						Alias
						Chr4:1345269_1347120
					
						Organism
						
							Cucumis sativus						
					
						Position
						
							chrChr4: 1345269-1347120  JBrowse»						
					
						Reference genome
						
							Cucumis sativus v1.5						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI2
					
						Parent gene
						
							Csa4G008220						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							Csa4G008220_circ_g.3 						
					
						Support reads
						NA
					
						Tissues
						leaf,root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Csa4G008220.1:3

General Information
CircRNA Name	Csa4G008220_circ_g.4
ID in PlantcircBase	csa_circ_002288
Alias	Chr4:1345269_1347120
Organism	Cucumis sativus
Position	chrChr4: 1345269-1347120 JBrowse»
Reference genome	Cucumis sativus v1.5
Type	e-circRNA
Identification method	CIRI2
Parent gene	Csa4G008220
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	Csa4G008220_circ_g.3
Support reads	NA
Tissues	leaf,root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Csa4G008220.1:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GTGAAGCTTGTACAAGTCTGATAAACAGAATATGCATTTTATCAGCAAACCTAGTCACTCTGAT
CCAATATCAGTGAAGGAGAAGTTCATTCATGCAAAGtgtcttgcgggtagtcccatgggaagtg
gtcatcacagatctactagtgagagtagttcttttgaaagttctgactttgatcagactgcggt
tgaagaat

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 579
Transcript exons: 1345269-1345607,1346770-1346915,1347027-1347120
TGTCTTGCGGGTAGTCCCATGGGAAGTGGTCATCACAGATCTACTAGTGAGAGTAGTTCTTTTG
AAAGTTCTGACTTTGATCAGACTGCGGTTGAAGAATACACATCGGAAAGAAGCCTTAGTTCCTC
TCATATGGACCGCCCTTCAAGAAACTTGCAACAGCAACGATCAGTGATGAAAATTGAGAAGCCA
ATAGACATACTTCGTAGAGTATGCGGGAACGATAAGTGTGCAGATTGTGGTGCTCCTGAACCTG
ACTGGGCATCTTTGAATCTTGGTGTTCTTGTTTGTATTGAATGTTCTGGTGTTCATCGTAATCT
TGGTGTTCACATATCAAAGGTGAGATCCCTTACATTGGATGTCAAAGTGTGGGAACCTTCAGTT
ATCAGTTTGTTTCAATCCCTTGGAAATACCTTTGCGAACTCGGTTTGGGAGGAGATGCTGCAAT
CTAGAAGTGCCTTTCAGGTCGATCTTGTTACGGCAGGCTTGTACAAGTCTGATAAACAGAATAT
GCATTTTATCAGCAAACCTAGTCACTCTGATCCAATATCAGTGAAGGAGAAGTTCATTCATGCA
AAG
						
					

						Genomic sequence
						
							TGTCTTGCGGGTAGTCCCATGGGAAGTGGTCATCACAGATCTACTAGTGAGAGTAGTTCTTTTG
AAAGTTCTGACTTTGATCAGACTGCGGTTGAAGAATACACATCGGAAAGAAGCCTTAGTTCCTC
TCATATGGACCGCCCTTCAAGAAACTTGCAACAGCAACGATCAGTGATGAAAATTGAGAAGCCA
ATAGACATACTTCGTAGAGTATGCGGGAACGATAAGTGTGCAGATTGTGGTGCTCCTGAACCTG
ACTGGGCATCTTTGAATCTTGGTGTTCTTGTTTGTATTGAATGTTCTGGTGTTCATCGTAATCT
TGGTGTTCACATATCAAAGGTATCTTGAACTTGCTTTTCATGCACTTTATCCAGTAGTGGTGTA
TCCTGTGGGTGATCTTCTACCATTAAGAAAGCATCATGCTATTATAAAAGGGCAGTGTATGCAT
GCGATATATGTAATTATCTTGTAATGATTTATTAAAAATGTGAGTTAATTCCATGACAATGTTC
TGAGTGGCATGAAAGCAGTATCCAAATGGCTGTTGGGTAATCTGACAAGCCTAGCATGATAAAA
CTTTTGTGGAACTAACCATGTAGTTGAAGAAATTCACTCAGTATTCGTGTTGTAGTTCATGTAA
CCAGGAGCAAATAAAACAATCATTTGTTTCCTGATCTTTTGTCGTGTTCATAATTCTGTTTGAG
TAGATGAGGTGCTAAAAAACTTGGCAGTTCCATATGTGAAATAAAGGGTTCCTAGTGGAAAGTA
ATGTGGGAAGAACTCATATAAATGACTGGTGCTGATGTTGCCTAAGTGGTCTTTAGGAGAATCC
TCCGAGAACCAAGATTGTTAATCTTTTTATTAGAATGAATAATGTGTTTCATATGAGAGGGGAA
TACACCTATTTATAGAGTTATTACGAGTGGGGATGGAAAGGAAATTATTTAGAATATTAATTAG
TTATCGAGTTAACCCCTCCTTCTTACATCAGCTGTTTTACGTCAGCCCCTGTTTTTTTGTTAAT
CTCATTGTGGTTTTCCTTTGTGAAATGGTGTTGATTATTGTTAACGCTTCATTGTAAATTTGTT
GTACTTCTTTACCGTTAAAGGTCAATGTGTGCAGCCATAAGTAAGACAATTTTACATCACAATA
GTTTGATTACTAGCTTTCATATAATTACCTTATAATAGGGCCTTTGGTATTCATTTCACCACAT
CAATGATATGCTTTTGTTCCATTTAAACAGAATAGTTTTATAACCTTTATATGATATTGCATTT
ATTGTCCTGTTTACAGGAAGTATGTGAATGTGTTCCTTGTTGCTTTTTAAAAGTGAAAAAAATT
AACCTAGAAATTAATTGGGTTAGTACACAAGATTGCTAAAAGTAAAGCTTTTCACTCGGTGGTG
CCCTTGGTTTTTGATAAAGATATTTCTGTGGTTAGTAATTCTTGTTGCTTCCTGCATTCTAAAT
GCTTACGGCTTACCTCTGCAATTGTGTAGGTGAGATCCCTTACATTGGATGTCAAAGTGTGGGA
ACCTTCAGTTATCAGTTTGTTTCAATCCCTTGGAAATACCTTTGCGAACTCGGTTTGGGAGGAG
ATGCTGCAATCTAGAAGTGCCTTTCAGGTCGATCTTGTTACGGCAGGGTAAATGAACTTCACTA
TTCAGTCAGTCATAAGTTTTTCAAGACAATGTTCAATATCCACTTTCATTTTATTTGGTTTTGC
TAACATTCACCTATTATTTTTGTGGTGAAGCTTGTACAAGTCTGATAAACAGAATATGCATTTT
ATCAGCAAACCTAGTCACTCTGATCCAATATCAGTGAAGGAGAAGTTCATTCATGCAAAG
						
					

			
		

Sequences
Splice junction sequence	GTGAAGCTTGTACAAGTCTGATAAACAGAATATGCATTTTATCAGCAAACCTAGTCACTCTGAT CCAATATCAGTGAAGGAGAAGTTCATTCATGCAAAGtgtcttgcgggtagtcccatgggaagtg gtcatcacagatctactagtgagagtagttcttttgaaagttctgactttgatcagactgcggt tgaagaat
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 579 Transcript exons: 1345269-1345607,1346770-1346915,1347027-1347120 TGTCTTGCGGGTAGTCCCATGGGAAGTGGTCATCACAGATCTACTAGTGAGAGTAGTTCTTTTG AAAGTTCTGACTTTGATCAGACTGCGGTTGAAGAATACACATCGGAAAGAAGCCTTAGTTCCTC TCATATGGACCGCCCTTCAAGAAACTTGCAACAGCAACGATCAGTGATGAAAATTGAGAAGCCA ATAGACATACTTCGTAGAGTATGCGGGAACGATAAGTGTGCAGATTGTGGTGCTCCTGAACCTG ACTGGGCATCTTTGAATCTTGGTGTTCTTGTTTGTATTGAATGTTCTGGTGTTCATCGTAATCT TGGTGTTCACATATCAAAGGTGAGATCCCTTACATTGGATGTCAAAGTGTGGGAACCTTCAGTT ATCAGTTTGTTTCAATCCCTTGGAAATACCTTTGCGAACTCGGTTTGGGAGGAGATGCTGCAAT CTAGAAGTGCCTTTCAGGTCGATCTTGTTACGGCAGGCTTGTACAAGTCTGATAAACAGAATAT GCATTTTATCAGCAAACCTAGTCACTCTGATCCAATATCAGTGAAGGAGAAGTTCATTCATGCA AAG
Genomic sequence	TGTCTTGCGGGTAGTCCCATGGGAAGTGGTCATCACAGATCTACTAGTGAGAGTAGTTCTTTTG AAAGTTCTGACTTTGATCAGACTGCGGTTGAAGAATACACATCGGAAAGAAGCCTTAGTTCCTC TCATATGGACCGCCCTTCAAGAAACTTGCAACAGCAACGATCAGTGATGAAAATTGAGAAGCCA ATAGACATACTTCGTAGAGTATGCGGGAACGATAAGTGTGCAGATTGTGGTGCTCCTGAACCTG ACTGGGCATCTTTGAATCTTGGTGTTCTTGTTTGTATTGAATGTTCTGGTGTTCATCGTAATCT TGGTGTTCACATATCAAAGGTATCTTGAACTTGCTTTTCATGCACTTTATCCAGTAGTGGTGTA TCCTGTGGGTGATCTTCTACCATTAAGAAAGCATCATGCTATTATAAAAGGGCAGTGTATGCAT GCGATATATGTAATTATCTTGTAATGATTTATTAAAAATGTGAGTTAATTCCATGACAATGTTC TGAGTGGCATGAAAGCAGTATCCAAATGGCTGTTGGGTAATCTGACAAGCCTAGCATGATAAAA CTTTTGTGGAACTAACCATGTAGTTGAAGAAATTCACTCAGTATTCGTGTTGTAGTTCATGTAA CCAGGAGCAAATAAAACAATCATTTGTTTCCTGATCTTTTGTCGTGTTCATAATTCTGTTTGAG TAGATGAGGTGCTAAAAAACTTGGCAGTTCCATATGTGAAATAAAGGGTTCCTAGTGGAAAGTA ATGTGGGAAGAACTCATATAAATGACTGGTGCTGATGTTGCCTAAGTGGTCTTTAGGAGAATCC TCCGAGAACCAAGATTGTTAATCTTTTTATTAGAATGAATAATGTGTTTCATATGAGAGGGGAA TACACCTATTTATAGAGTTATTACGAGTGGGGATGGAAAGGAAATTATTTAGAATATTAATTAG TTATCGAGTTAACCCCTCCTTCTTACATCAGCTGTTTTACGTCAGCCCCTGTTTTTTTGTTAAT CTCATTGTGGTTTTCCTTTGTGAAATGGTGTTGATTATTGTTAACGCTTCATTGTAAATTTGTT GTACTTCTTTACCGTTAAAGGTCAATGTGTGCAGCCATAAGTAAGACAATTTTACATCACAATA GTTTGATTACTAGCTTTCATATAATTACCTTATAATAGGGCCTTTGGTATTCATTTCACCACAT CAATGATATGCTTTTGTTCCATTTAAACAGAATAGTTTTATAACCTTTATATGATATTGCATTT ATTGTCCTGTTTACAGGAAGTATGTGAATGTGTTCCTTGTTGCTTTTTAAAAGTGAAAAAAATT AACCTAGAAATTAATTGGGTTAGTACACAAGATTGCTAAAAGTAAAGCTTTTCACTCGGTGGTG CCCTTGGTTTTTGATAAAGATATTTCTGTGGTTAGTAATTCTTGTTGCTTCCTGCATTCTAAAT GCTTACGGCTTACCTCTGCAATTGTGTAGGTGAGATCCCTTACATTGGATGTCAAAGTGTGGGA ACCTTCAGTTATCAGTTTGTTTCAATCCCTTGGAAATACCTTTGCGAACTCGGTTTGGGAGGAG ATGCTGCAATCTAGAAGTGCCTTTCAGGTCGATCTTGTTACGGCAGGGTAAATGAACTTCACTA TTCAGTCAGTCATAAGTTTTTCAAGACAATGTTCAATATCCACTTTCATTTTATTTGGTTTTGC TAACATTCACCTATTATTTTTGTGGTGAAGCTTGTACAAGTCTGATAAACAGAATATGCATTTT ATCAGCAAACCTAGTCACTCTGATCCAATATCAGTGAAGGAGAAGTTCATTCATGCAAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.512343782

Conservation Information
Conserved circRNAs	NA
PMCS	0.512343782

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							1345288-1347117(+)
1347114-1345440(+)
						
						Potential amino acid sequence
						
							MGSGHHRSTSESSSFESSDFDQTAVEEYTSERSLSSSHMDRPSRNLQQQRSVMKIEKPIDILRR
VCGNDKCADCGAPEPDWASLNLGVLVCIECSGVHRNLGVHISKVRSLTLDVKVWEPSVISLFQS
LGNTFANSVWEEMLQSRSAFQVDLVTAGLYKSDKQNMHFISKPSHSDPISVKEKFIHAKCLAGS
PMGSGHHRSTSESSSFESSDFDQTAVEEYTSERSLSSSHMDRPSRNLQQQRSVMKIEKPIDILR
RVCGNDKCADCGAPEPDWASLNLGVLVCIECSGVHRNLGVHISKVRSLTLDVKVWEPSVISLFQ
SLGNTFANSVWEEMLQSRSAFQVDLVTAGLYKSDKQNMHFISKPSHSDPISVKEKFIHAKCLAG
SPMGSGHHRSTSESSSFESSDFDQTAVEEYTSERSLSSSHMDRPSRNLQQQRSVMKIEKPIDIL
RRVCGNDKCADCGAPEPDWASLNLGVLVCIECSGVHRNLGVHISKVRSLTLDVKVWEPSVISLF
QSLGNTFANSVWEEMLQSRSAFQVDLVTAGLYKSDKQNMHFISKPSHSDPISVKEKFIHAKCLA
GSPMGSGHHRSTSESSSFESSDFDQTAVEEYTSERSLSSSHMDRPSRNLQQQRSVMKIEKPIDI
LRRVCGNDKCADCGAPEPDWASLNLGVLVCIECSGVHRNLGVHISKVRSLTLDVKVWEPSVISL
FQSLGNTFANSVWEEMLQSRSAFQVDLVTAGLYKSDKQNMHFISKPSHSDPISVKEKFIHAK
MQSVLRVVPWEVVITDLLVRVVLLKVLTLIRLRLKNTHRKEALVPLIWTALQETCNSNDQ*
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to salt stress in root

Functional Information
Coding potential	Y
Potential coding position	1345288-1347117(+) 1347114-1345440(+)
Potential amino acid sequence	MGSGHHRSTSESSSFESSDFDQTAVEEYTSERSLSSSHMDRPSRNLQQQRSVMKIEKPIDILRR VCGNDKCADCGAPEPDWASLNLGVLVCIECSGVHRNLGVHISKVRSLTLDVKVWEPSVISLFQS LGNTFANSVWEEMLQSRSAFQVDLVTAGLYKSDKQNMHFISKPSHSDPISVKEKFIHAKCLAGS PMGSGHHRSTSESSSFESSDFDQTAVEEYTSERSLSSSHMDRPSRNLQQQRSVMKIEKPIDILR RVCGNDKCADCGAPEPDWASLNLGVLVCIECSGVHRNLGVHISKVRSLTLDVKVWEPSVISLFQ SLGNTFANSVWEEMLQSRSAFQVDLVTAGLYKSDKQNMHFISKPSHSDPISVKEKFIHAKCLAG SPMGSGHHRSTSESSSFESSDFDQTAVEEYTSERSLSSSHMDRPSRNLQQQRSVMKIEKPIDIL RRVCGNDKCADCGAPEPDWASLNLGVLVCIECSGVHRNLGVHISKVRSLTLDVKVWEPSVISLF QSLGNTFANSVWEEMLQSRSAFQVDLVTAGLYKSDKQNMHFISKPSHSDPISVKEKFIHAKCLA GSPMGSGHHRSTSESSSFESSDFDQTAVEEYTSERSLSSSHMDRPSRNLQQQRSVMKIEKPIDI LRRVCGNDKCADCGAPEPDWASLNLGVLVCIECSGVHRNLGVHISKVRSLTLDVKVWEPSVISL FQSLGNTFANSVWEEMLQSRSAFQVDLVTAGLYKSDKQNMHFISKPSHSDPISVKEKFIHAK MQSVLRVVPWEVVITDLLVRVVLLKVLTLIRLRLKNTHRKEALVPLIWTALQETCNSNDQ*
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to salt stress in root

			
						 Other Information
					
						References
						Zhu et al., 2019

circRNA

Detail information of Csa4G008220_circ_g.4

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