Plant Circular RNA Database

Detail information of Os12g0238100_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os12g0238100_circ_g.3
					
						ID in PlantcircBase
						osa_circ_010937
					
						Alias
						Os_ciR7583
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr12: 7635217-7635935  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os12g0238100						
					
						Parent gene annotation
						
							Exocyst complex component Sec10 family protein. (Os12t0238100-01
);Similar to Exocyst complex component 5. (Os12t0238100-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os12g0238100_circ_g.4 Os12g0238100_circ_g.5 Os12g0238100_circ_g.6 						
					
						Support reads
						2/1
					
						Tissues
						root/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os12t0238100-01:4
Os12t0238100-02:1

General Information
CircRNA Name	Os12g0238100_circ_g.3
ID in PlantcircBase	osa_circ_010937
Alias	Os_ciR7583
Organism	Oryza sativa
Position	chr12: 7635217-7635935 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os12g0238100
Parent gene annotation	Exocyst complex component Sec10 family protein. (Os12t0238100-01 );Similar to Exocyst complex component 5. (Os12t0238100-02)
Parent gene strand	-
Alternative splicing	Os12g0238100_circ_g.4 Os12g0238100_circ_g.5 Os12g0238100_circ_g.6
Support reads	2/1
Tissues	root/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os12t0238100-01:4 Os12t0238100-02:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CACCAAGAACAACTTGAATATCAATGCTCATAATATCCACATCAATAAACATTGGCCGTGTTGC
AACATAATGTTGCATAGCACTGGTTCCGCGGTTGAActtcaatgtccaaatccccacaactgat
agattgcaactctttagcaagttcttttgtcttgtcatatgctaccgccagaactctgagatac
tgccattt

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 436
Transcript exons: 7635217-7635307,7635416-7635514,7635601-7635677,7635767-
7635935
CTTCAATGTCCAAATCCCCACAACTGATAGATTGCAACTCTTTAGCAAGTTCTTTTGTCTTGTC
ATATGCTACCGCCAGAACTCTGAGATAATGCAGAAGACCACCCTCTTCTATAGGAGGTAAGTTA
GCAAGAGAGGGCTTAATAAGAAGTTTATCAAGAATTGCTGTTACCCTTTGTTCTAAAACTCTCT
GCACAAGAATTGACATGACCTCATTTGGAGAAGGAAATACAGCCATTATTGTAGTTGCTTCTCT
CCTCACAGTATCTGCAATTTCCTTGTACAATACAGAGAGGCCCTCTGCAATACAAATGTGGTCA
GCTTGTGGCCCCTCCTCACCAAGAACAACTTGAATATCAATGCTCATAATATCCACATCAATAA
ACATTGGCCGTGTTGCAACATAATGTTGCATAGCACTGGTTCCGCGGTTGAA
						
						Genomic sequence
						
							CTTCAATGTCCAAATCCCCACAACTGATAGATTGCAACTCTTTAGCAAGTTCTTTTGTCTTGTC
ATATGCTACCGCCAGAACTCTGAGATACTGCCATTTCCATTTGATAGGAATGAGATCTATCATG
CTATGGTAAAGGATTGGCAATATATATATTTATATATAATTTGGAAGTATAATATTGTACACGG
TGCTTACATGCAGAAGACCACCCTCTTCTATAGGAGGTAAGTTAGCAAGAGAGGGCTTAATAAG
AAGTTTATCAAGAATTGCTGTTACCCTTTGTTCTAAAACTCTCTGAAAAGCAAAGTACAGTAGT
CTTACTTTATGCATGTCATAAACACATGGTCATCACTTAGAAATCACAGCTCAAGGAGACATAC
CTGCACAAGAATTGACATGACCTCATTTGGAGAAGGAAATACAGCCATTATTGTAGTTGCTTCT
CTCCTCACAGTATCTGAAGCAGCAGTATGATTGTCAGATTACTATAAATGTATATCTTTGAACT
ATCAACTTGTAAACAATAATCATCAAACTTTTCACTACCTGCAATTTCCTTGTACAATACAGAG
AGGCCCTCTGCAATACAAATGTGGTCAGCTTGTGGCCCCTCCTCACCAAGAACAACTTGAATAT
CAATGCTCATAATATCCACATCAATAAACATTGGCCGTGTTGCAACATAATGTTGCATAGCACT
GGTTCCGCGGTTGAA

Sequences
Splice junction sequence	CACCAAGAACAACTTGAATATCAATGCTCATAATATCCACATCAATAAACATTGGCCGTGTTGC AACATAATGTTGCATAGCACTGGTTCCGCGGTTGAActtcaatgtccaaatccccacaactgat agattgcaactctttagcaagttcttttgtcttgtcatatgctaccgccagaactctgagatac tgccattt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 436 Transcript exons: 7635217-7635307,7635416-7635514,7635601-7635677,7635767- 7635935 CTTCAATGTCCAAATCCCCACAACTGATAGATTGCAACTCTTTAGCAAGTTCTTTTGTCTTGTC ATATGCTACCGCCAGAACTCTGAGATAATGCAGAAGACCACCCTCTTCTATAGGAGGTAAGTTA GCAAGAGAGGGCTTAATAAGAAGTTTATCAAGAATTGCTGTTACCCTTTGTTCTAAAACTCTCT GCACAAGAATTGACATGACCTCATTTGGAGAAGGAAATACAGCCATTATTGTAGTTGCTTCTCT CCTCACAGTATCTGCAATTTCCTTGTACAATACAGAGAGGCCCTCTGCAATACAAATGTGGTCA GCTTGTGGCCCCTCCTCACCAAGAACAACTTGAATATCAATGCTCATAATATCCACATCAATAA ACATTGGCCGTGTTGCAACATAATGTTGCATAGCACTGGTTCCGCGGTTGAA
Genomic sequence	CTTCAATGTCCAAATCCCCACAACTGATAGATTGCAACTCTTTAGCAAGTTCTTTTGTCTTGTC ATATGCTACCGCCAGAACTCTGAGATACTGCCATTTCCATTTGATAGGAATGAGATCTATCATG CTATGGTAAAGGATTGGCAATATATATATTTATATATAATTTGGAAGTATAATATTGTACACGG TGCTTACATGCAGAAGACCACCCTCTTCTATAGGAGGTAAGTTAGCAAGAGAGGGCTTAATAAG AAGTTTATCAAGAATTGCTGTTACCCTTTGTTCTAAAACTCTCTGAAAAGCAAAGTACAGTAGT CTTACTTTATGCATGTCATAAACACATGGTCATCACTTAGAAATCACAGCTCAAGGAGACATAC CTGCACAAGAATTGACATGACCTCATTTGGAGAAGGAAATACAGCCATTATTGTAGTTGCTTCT CTCCTCACAGTATCTGAAGCAGCAGTATGATTGTCAGATTACTATAAATGTATATCTTTGAACT ATCAACTTGTAAACAATAATCATCAAACTTTTCACTACCTGCAATTTCCTTGTACAATACAGAG AGGCCCTCTGCAATACAAATGTGGTCAGCTTGTGGCCCCTCCTCACCAAGAACAACTTGAATAT CAATGCTCATAATATCCACATCAATAAACATTGGCCGTGTTGCAACATAATGTTGCATAGCACT GGTTCCGCGGTTGAA

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_010306 						
					
						PMCS
						0.453631073

Conservation Information
Conserved circRNAs	osi_circ_010306
PMCS	0.453631073

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							7635916-7635879(-)
7635284-7635879(-)
						
						Potential amino acid sequence
						
							MQHYVATRPMFIDVDIMSIDIQVVLGEEGPQADHICIAEGLSVLYKEIADTVRREATTIMAVFP
SPNEVMSILVQRVLEQRVTAILDKLLIKPSLANLPPIEEGGLLHYLRVLAVAYDKTKELAKELQ
SISCGDLDIEVQPRNQCYATLCCNTANVY*(-)
MTRQKNLLKSCNLSVVGIWTLKFNRGTSAMQHYVATRPMFIDVDIMSIDIQVVLGEEGPQADHI
CIAEGLSVLYKEIADTVRREATTIMAVFPSPNEVMSILVQRVLEQRVTAILDKLLIKPSLANLP
PIEEGGLLHYLRVLAVAYDKTKELAKELQSISCGDLDIEVQPRNQCYATLCCNTANVY*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	7635916-7635879(-) 7635284-7635879(-)
Potential amino acid sequence	MQHYVATRPMFIDVDIMSIDIQVVLGEEGPQADHICIAEGLSVLYKEIADTVRREATTIMAVFP SPNEVMSILVQRVLEQRVTAILDKLLIKPSLANLPPIEEGGLLHYLRVLAVAYDKTKELAKELQ SISCGDLDIEVQPRNQCYATLCCNTANVY(-) MTRQKNLLKSCNLSVVGIWTLKFNRGTSAMQHYVATRPMFIDVDIMSIDIQVVLGEEGPQADHI CIAEGLSVLYKEIADTVRREATTIMAVFPSPNEVMSILVQRVLEQRVTAILDKLLIKPSLANLP PIEEGGLLHYLRVLAVAYDKTKELAKELQSISCGDLDIEVQPRNQCYATLCCNTANVY(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os12g0238100_circ_g.3

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