Plant Circular RNA Database

Detail information of AT2G14050_circ_g.7

			
						 General Information
					
						CircRNA Name
						AT2G14050_circ_g.7
					
						ID in PlantcircBase
						ath_circ_013298
					
						Alias
						At_ciR401, AT2G14050_C1
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr2: 5912593-5913797  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, CIRI, MapSplice, circRNA_finder, find_circ, CIRI-full, CIRI2
					
						Parent gene
						
							AT2G14050						
					
						Parent gene annotation
						
							minichromosome maintenance 9
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT2G14045_circ_g.1 AT2G14045_circ_g.2 AT2G14045_circ_g.3 2_circ_igg.4 AT2G14045_circ_g.5 AT2G14050_circ_g.1 AT2G14050_circ_g.2 AT2G14050_circ_g.3 AT2G14050_circ_g.4 AT2G14050_circ_g.5 AT2G14050_circ_g.6 AT2G14050_circ_g.8 						
					
						Support reads
						6/12/20
					
						Tissues
						leaf/root, aerial, whole_plant/seedlings
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT2G14050.1:5
AT2G14050.2:5

General Information
CircRNA Name	AT2G14050_circ_g.7
ID in PlantcircBase	ath_circ_013298
Alias	At_ciR401, AT2G14050_C1
Organism	Arabidpsis thaliana
Position	chr2: 5912593-5913797 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, CIRI, MapSplice, circRNA_finder, find_circ, CIRI-full, CIRI2
Parent gene	AT2G14050
Parent gene annotation	minichromosome maintenance 9
Parent gene strand	+
Alternative splicing	AT2G14045_circ_g.1 AT2G14045_circ_g.2 AT2G14045_circ_g.3 2_circ_igg.4 AT2G14045_circ_g.5 AT2G14050_circ_g.1 AT2G14050_circ_g.2 AT2G14050_circ_g.3 AT2G14050_circ_g.4 AT2G14050_circ_g.5 AT2G14050_circ_g.6 AT2G14050_circ_g.8
Support reads	6/12/20
Tissues	leaf/root, aerial, whole_plant/seedlings
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT2G14050.1:5 AT2G14050.2:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GCTATCTTGTGCATTGAATCATCGATGACTATTTCAGCCATAGTAGACAGTATGGGGAATGCGC
TACACTCAAATTTCTCCGAAGAACCAGATCAGGAATctctttctgtcaatactgcactttccgg
tcccttgttgagcaggtttgacattgtacttgttctcttagatacaaaaaatcctgaatgggat
gcagttgt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 138
Transcript exons: 5912593-5912621,5913689-5913797
CTCTTTCTGTCAATACTGCACTTTCCGGTGCCATAACAGCTATCTTGTGCATTGAATCATCGAT
GACTATTTCAGCCATAGTAGACAGTATGGGGAATGCGCTACACTCAAATTTCTCCGAAGAACCA
GATCAGGAAT
Transcript length: 147
Transcript exons: 5912593-5912632,5913691-5913797
CTCTTTCTGTCAATACTGCACTTTCCGGTCCCTTGTTGAGCATAACAGCTATCTTGTGCATTGA
ATCATCGATGACTATTTCAGCCATAGTAGACAGTATGGGGAATGCGCTACACTCAAATTTCTCC
GAAGAACCAGATCAGGAAT
Transcript length: 501
Transcript exons: 5912593-5912714,5912840-5912907,5913058-5913167,5913507-
5913554,5913645-5913797
CTCTTTCTGTCAATACTGCACTTTCCGGTCCCTTGTTGAGCAGGTTTGACATTGTACTTGTTCT
CTTAGATACAAAAAATCCTGAATGGGATGCAGTTGTATCTTCTCACATCCTTGCCGAGGTGCAA
ATAGAGCAAGACAGAGAGGTCGATGATCTGACAACTATTTGGCCACTACCGATGCTTCAAAGAT
ACATTCAGTTTGTGAAAAAAAACTTCAGACCTGTCCTATCAAAGGAAGCAGAAGAAATAATTTC
AAGTTATTATCGACTCCAAAGAAGATCTTCAACTCATAATGCAGCAAGAACAACCGTGCGGATG
TTGGAAAGCTTAATCCGTTTGGCTCAAGCTCATGCAAGGCTTATGTTCAGAAATGAGGTCACGC
GATTAGATGCCATAACAGCTATCTTGTGCATTGAATCATCGATGACTATTTCAGCCATAGTAGA
CAGTATGGGGAATGCGCTACACTCAAATTTCTCCGAAGAACCAGATCAGGAAT
Transcript length: 1229
Transcript exons: 5912593-5913797
CTCTTTCTGTCAATACTGCACTTTCCGGTCCCTTGTTGAGCAGGTTTGACATTGTACTTGTTCT
CTTAGATACAAAAAATCCTGAATGGGATGCAGTTGTATCTTCTCACATCCTTGCCGAGGTGCAA
ATAGAGCAAGACAGAGAGGTCGATGATCTGACAACTATTTGGCCACTACCGATGCTTCAAAGAT
ACATTCAGTTTGTGAAAAAAAACTTCAGACCTGTCCTATCAAAGGAAGCAGAAGAAATAATTTC
AAGTTATTATCGACTCCAAAGAAGATCTTCAACTCATAATGCAGCAAGAACAACCGTGCGGATG
TTGGAAAGCTTAATCCGTTTGGCTCAAGCTCATGCAAGGCTTATGTTCAGAAATGAGGTCACGC
GATTAGATGCCATAACAGCTATCTTGTGCATTGAATCATCGATGACTATTTCAGCCATAGTAGA
CAGTATGGGGAATGCGCTACACTCAAATTTCTCCGAAGAACCAGATCAGGAATCTCTTTCTGTC
AATACTGCACTTTCCGGTCCCTTGTTGAGCAGGTTTGACATTGTACTTGTTCTCTTAGATACAA
AAAATCCTGAATGGGATGCAGTTGTATCTTCTCACATCCTTGCCGAGGTGCAAATAGAGCAAGA
CAGAGAGGTCGATGATCTGACAACTATTTGGCCACTACCGATGCTTCAAAGATACATTCAGTTT
GTGAAAAAAAACTTCAGACCTGTCCTATCAAAGGAAGCAGAAGAAATAATTTCAAGTTATTATC
GACTCCAAAGAAGATCTTCAACTCATAATGCAGCTCATGCAAGGCTTATGTTCAGAAATGAGGT
CACGCGATTAGATGCCATAACAGCTATCTTGTGCATTGAATCATCGATGACTATTTCAGCCATA
GTAGACAGTATGGGGAATGCGCTACACTCAAATTTCTCCGAAGAACCAGATCAGGAATCTCTTT
CTGTCAATACTGCACTTTCCGGTCCCTTGTTGAGCAGGTTTGACATTGTACTTGTTCTCTTAGA
TACAAAAAATCCTGAATGGGATGCAGTTGTATCTTCTCACATCCTTGCCGAGCTCATGCAAGGC
TTATGTTCAGAAATGAGGTCACGCGATTAGATGCCATAACAGCTATCTTGTGCATTGAATCATC
GATGACTATTTCAGCCATAGTAGACAGTATGGGGAATGCGCTACACTCAAATTTCTCCGAAGAA
CCAGATCAGGAAT
						
					

						Genomic sequence
						
							CTCTTTCTGTCAATACTGCACTTTCCGGTCCCTTGTTGAGCAGGTTTGACATTGTACTTGTTCT
CTTAGATACAAAAAATCCTGAATGGGATGCAGTTGTATCTTCTCACATCCTTGCCGAGGTAAGT
TTCTTTAAAGAATCTATAAATATTGTGTTGTATACTGATATTGAGTCTTCTTAAGTAGAGTATT
TGTGACAAGATATATAACTCATCTGACTTTTAAAAAATTGATGAAATGAACCAAGGTGCAAATA
GAGCAAGACAGAGAGGTCGATGATCTGACAACTATTTGGCCACTACCGATGCTTCAAAGGTATC
ATATACGTACACAGATCATGAGATCCATAACCCATGAGAGTGTCAATTATTCCTTGCACATAAA
TCCGGGACGACAAATGTGCTTGATCTTAGCTGATAATACATTCATGGTTGCTACTAACTTATTA
TATCCTGACCTTTGCAGATACATTCAGTTTGTGAAAAAAAACTTCAGACCTGTCCTATCAAAGG
AAGCAGAAGAAATAATTTCAAGTTATTATCGACTCCAAAGAAGATCTTCAACTCATAATGCAGG
TATCTCCTGAGAAATACTTCGTGGTTCTAGAGATGCTAGTTTGGTATTAACATGGGGTGAGGGA
AGCTGACTATAGTGGACCACTGTCCAGATTTGAATCCTATTATTTGATGGCTGAGACATTTTAT
TCCTTACTTATATAGAAAGTTGCTCCGAAACCCCTTCATATTGGAGGTACTAGGTCTAGAAAAT
CCTTAGGTCCCATGGGAACTCTGATTAAGCATAACCTTGCTTAAATGGATTTTAGGACTGGCTT
GGTGGGTACAACAGTATCAACTAAGATCGATCCTACAATGGTTGATATCTTCCATGTAATTTGA
TTTTACTCTGAACTGCAGCAAGAACAACCGTGCGGATGTTGGAAAGCTTAATCCGTTTGGCTCA
AGGTACCTCTTCTGTTATCGTCTTTATCAATCAAAATACTCCAAAAACAGGAACAAAAGAGAGG
ATATTAATGGTATTATGGTGTCACGCAGCTCATGCAAGGCTTATGTTCAGAAATGAGGTCACGC
GATTAGATGCCATAACAGCTATCTTGTGCATTGAATCATCGATGACTATTTCAGCCATAGTAGA
CAGTATGGGGAATGCGCTACACTCAAATTTCTCCGAAGAACCAGATCAGGAAT
						
					

			
		

Sequences
Splice junction sequence	GCTATCTTGTGCATTGAATCATCGATGACTATTTCAGCCATAGTAGACAGTATGGGGAATGCGC TACACTCAAATTTCTCCGAAGAACCAGATCAGGAATctctttctgtcaatactgcactttccgg tcccttgttgagcaggtttgacattgtacttgttctcttagatacaaaaaatcctgaatgggat gcagttgt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 138 Transcript exons: 5912593-5912621,5913689-5913797 CTCTTTCTGTCAATACTGCACTTTCCGGTGCCATAACAGCTATCTTGTGCATTGAATCATCGAT GACTATTTCAGCCATAGTAGACAGTATGGGGAATGCGCTACACTCAAATTTCTCCGAAGAACCA GATCAGGAAT Transcript length: 147 Transcript exons: 5912593-5912632,5913691-5913797 CTCTTTCTGTCAATACTGCACTTTCCGGTCCCTTGTTGAGCATAACAGCTATCTTGTGCATTGA ATCATCGATGACTATTTCAGCCATAGTAGACAGTATGGGGAATGCGCTACACTCAAATTTCTCC GAAGAACCAGATCAGGAAT Transcript length: 501 Transcript exons: 5912593-5912714,5912840-5912907,5913058-5913167,5913507- 5913554,5913645-5913797 CTCTTTCTGTCAATACTGCACTTTCCGGTCCCTTGTTGAGCAGGTTTGACATTGTACTTGTTCT CTTAGATACAAAAAATCCTGAATGGGATGCAGTTGTATCTTCTCACATCCTTGCCGAGGTGCAA ATAGAGCAAGACAGAGAGGTCGATGATCTGACAACTATTTGGCCACTACCGATGCTTCAAAGAT ACATTCAGTTTGTGAAAAAAAACTTCAGACCTGTCCTATCAAAGGAAGCAGAAGAAATAATTTC AAGTTATTATCGACTCCAAAGAAGATCTTCAACTCATAATGCAGCAAGAACAACCGTGCGGATG TTGGAAAGCTTAATCCGTTTGGCTCAAGCTCATGCAAGGCTTATGTTCAGAAATGAGGTCACGC GATTAGATGCCATAACAGCTATCTTGTGCATTGAATCATCGATGACTATTTCAGCCATAGTAGA CAGTATGGGGAATGCGCTACACTCAAATTTCTCCGAAGAACCAGATCAGGAAT Transcript length: 1229 Transcript exons: 5912593-5913797 CTCTTTCTGTCAATACTGCACTTTCCGGTCCCTTGTTGAGCAGGTTTGACATTGTACTTGTTCT CTTAGATACAAAAAATCCTGAATGGGATGCAGTTGTATCTTCTCACATCCTTGCCGAGGTGCAA ATAGAGCAAGACAGAGAGGTCGATGATCTGACAACTATTTGGCCACTACCGATGCTTCAAAGAT ACATTCAGTTTGTGAAAAAAAACTTCAGACCTGTCCTATCAAAGGAAGCAGAAGAAATAATTTC AAGTTATTATCGACTCCAAAGAAGATCTTCAACTCATAATGCAGCAAGAACAACCGTGCGGATG TTGGAAAGCTTAATCCGTTTGGCTCAAGCTCATGCAAGGCTTATGTTCAGAAATGAGGTCACGC GATTAGATGCCATAACAGCTATCTTGTGCATTGAATCATCGATGACTATTTCAGCCATAGTAGA CAGTATGGGGAATGCGCTACACTCAAATTTCTCCGAAGAACCAGATCAGGAATCTCTTTCTGTC AATACTGCACTTTCCGGTCCCTTGTTGAGCAGGTTTGACATTGTACTTGTTCTCTTAGATACAA AAAATCCTGAATGGGATGCAGTTGTATCTTCTCACATCCTTGCCGAGGTGCAAATAGAGCAAGA CAGAGAGGTCGATGATCTGACAACTATTTGGCCACTACCGATGCTTCAAAGATACATTCAGTTT GTGAAAAAAAACTTCAGACCTGTCCTATCAAAGGAAGCAGAAGAAATAATTTCAAGTTATTATC GACTCCAAAGAAGATCTTCAACTCATAATGCAGCTCATGCAAGGCTTATGTTCAGAAATGAGGT CACGCGATTAGATGCCATAACAGCTATCTTGTGCATTGAATCATCGATGACTATTTCAGCCATA GTAGACAGTATGGGGAATGCGCTACACTCAAATTTCTCCGAAGAACCAGATCAGGAATCTCTTT CTGTCAATACTGCACTTTCCGGTCCCTTGTTGAGCAGGTTTGACATTGTACTTGTTCTCTTAGA TACAAAAAATCCTGAATGGGATGCAGTTGTATCTTCTCACATCCTTGCCGAGCTCATGCAAGGC TTATGTTCAGAAATGAGGTCACGCGATTAGATGCCATAACAGCTATCTTGTGCATTGAATCATC GATGACTATTTCAGCCATAGTAGACAGTATGGGGAATGCGCTACACTCAAATTTCTCCGAAGAA CCAGATCAGGAAT
Genomic sequence	CTCTTTCTGTCAATACTGCACTTTCCGGTCCCTTGTTGAGCAGGTTTGACATTGTACTTGTTCT CTTAGATACAAAAAATCCTGAATGGGATGCAGTTGTATCTTCTCACATCCTTGCCGAGGTAAGT TTCTTTAAAGAATCTATAAATATTGTGTTGTATACTGATATTGAGTCTTCTTAAGTAGAGTATT TGTGACAAGATATATAACTCATCTGACTTTTAAAAAATTGATGAAATGAACCAAGGTGCAAATA GAGCAAGACAGAGAGGTCGATGATCTGACAACTATTTGGCCACTACCGATGCTTCAAAGGTATC ATATACGTACACAGATCATGAGATCCATAACCCATGAGAGTGTCAATTATTCCTTGCACATAAA TCCGGGACGACAAATGTGCTTGATCTTAGCTGATAATACATTCATGGTTGCTACTAACTTATTA TATCCTGACCTTTGCAGATACATTCAGTTTGTGAAAAAAAACTTCAGACCTGTCCTATCAAAGG AAGCAGAAGAAATAATTTCAAGTTATTATCGACTCCAAAGAAGATCTTCAACTCATAATGCAGG TATCTCCTGAGAAATACTTCGTGGTTCTAGAGATGCTAGTTTGGTATTAACATGGGGTGAGGGA AGCTGACTATAGTGGACCACTGTCCAGATTTGAATCCTATTATTTGATGGCTGAGACATTTTAT TCCTTACTTATATAGAAAGTTGCTCCGAAACCCCTTCATATTGGAGGTACTAGGTCTAGAAAAT CCTTAGGTCCCATGGGAACTCTGATTAAGCATAACCTTGCTTAAATGGATTTTAGGACTGGCTT GGTGGGTACAACAGTATCAACTAAGATCGATCCTACAATGGTTGATATCTTCCATGTAATTTGA TTTTACTCTGAACTGCAGCAAGAACAACCGTGCGGATGTTGGAAAGCTTAATCCGTTTGGCTCA AGGTACCTCTTCTGTTATCGTCTTTATCAATCAAAATACTCCAAAAACAGGAACAAAAGAGAGG ATATTAATGGTATTATGGTGTCACGCAGCTCATGCAAGGCTTATGTTCAGAAATGAGGTCACGC GATTAGATGCCATAACAGCTATCTTGTGCATTGAATCATCGATGACTATTTCAGCCATAGTAGA CAGTATGGGGAATGCGCTACACTCAAATTTCTCCGAAGAACCAGATCAGGAAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.630500896

Conservation Information
Conserved circRNAs	NA
PMCS	0.630500896

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							5912897-5913794(+)
						
						Potential amino acid sequence
						
							MLQRYIQFVKKNFRPVLSKEAEEIISSYYRLQRRSSTHNAARTTVRMLESLIRLAQAHARLMFR
NEVTRLDAITAILCIESSMTISAIVDSMGNALHSNFSEEPDQESLSVNTALSGPLLSRFDIVLV
LLDTKNPEWDAVVSSHILAEVQIEQDREVDDLTTIWPLPMLQRYIQFVKKNFRPVLSKEAEEII
SSYYRLQRRSSTHNAARTTVRMLESLIRLAQAHARLMFRNEVTRLDAITAILCIESSMTISAIV
DSMGNALHSNFSEEPDQESLSVNTALSGPLLSRFDIVLVLLDTKNPEWDAVVSSHILAEVQIEQ
DREVDDLTTIWPLPMLQRYIQFVKKNFRPVLSKEAEEIISSYYRLQRRSSTHNAARTTVRMLES
LIRLAQAHARLMFRNEVTRLDAITAILCIESSMTISAIVDSMGNALHSNFSEEPDQESLSVNTA
LSGPLLSRFDIVLVLLDTKNPEWDAVVSSHILAEVQIEQDREVDDLTTIWPLPMLQRYIQFVKK
NFRPVLSKEAEEIISSYYRLQRRSSTHNAARTTVRMLESLIRLAQAHARLMFRNEVTRLDAITA
ILCIESSMTISAIVDSMGNALHSNFSEEPDQE(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to heat stress

Functional Information
Coding potential	Y
Potential coding position	5912897-5913794(+)
Potential amino acid sequence	MLQRYIQFVKKNFRPVLSKEAEEIISSYYRLQRRSSTHNAARTTVRMLESLIRLAQAHARLMFR NEVTRLDAITAILCIESSMTISAIVDSMGNALHSNFSEEPDQESLSVNTALSGPLLSRFDIVLV LLDTKNPEWDAVVSSHILAEVQIEQDREVDDLTTIWPLPMLQRYIQFVKKNFRPVLSKEAEEII SSYYRLQRRSSTHNAARTTVRMLESLIRLAQAHARLMFRNEVTRLDAITAILCIESSMTISAIV DSMGNALHSNFSEEPDQESLSVNTALSGPLLSRFDIVLVLLDTKNPEWDAVVSSHILAEVQIEQ DREVDDLTTIWPLPMLQRYIQFVKKNFRPVLSKEAEEIISSYYRLQRRSSTHNAARTTVRMLES LIRLAQAHARLMFRNEVTRLDAITAILCIESSMTISAIVDSMGNALHSNFSEEPDQESLSVNTA LSGPLLSRFDIVLVLLDTKNPEWDAVVSSHILAEVQIEQDREVDDLTTIWPLPMLQRYIQFVKK NFRPVLSKEAEEIISSYYRLQRRSSTHNAARTTVRMLESLIRLAQAHARLMFRNEVTRLDAITA ILCIESSMTISAIVDSMGNALHSNFSEEPDQE(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to heat stress

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017;Pan et al., 2017;Liu et al., 2017;Zhang et al., 2019

circRNA

Detail information of AT2G14050_circ_g.7

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