Plant Circular RNA Database

Detail information of AT5G19550_circ_g.3

			
						 General Information
					
						CircRNA Name
						AT5G19550_circ_g.3
					
						ID in PlantcircBase
						ath_circ_039251
					
						Alias
						At_ciR408, AT5G19550_C1
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 6598597-6599463  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, MapSplice, PcircRNA_finder, circRNA_finder, find_circ, CIRI-full, CIRI2
					
						Parent gene
						
							AT5G19550						
					
						Parent gene annotation
						
							Aspartate aminotransferase
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT5G19550_circ_g.2 AT5G19550_circ_g.4 AT5G19550_circ_g.5 AT5G19550_circ_g.6 AT5G19550_circ_g.7 AT5G19550_circ_g.8 AT5G19550_circ_g.9 AT5G19550_circ_g.10 AT5G19550_circ_g.11 AT5G19550_circ_g.12 AT5G19550_circ_g.13 						
					
						Support reads
						5/4
					
						Tissues
						leaf/whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G19550.1:5

General Information
CircRNA Name	AT5G19550_circ_g.3
ID in PlantcircBase	ath_circ_039251
Alias	At_ciR408, AT5G19550_C1
Organism	Arabidpsis thaliana
Position	chr5: 6598597-6599463 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, MapSplice, PcircRNA_finder, circRNA_finder, find_circ, CIRI-full, CIRI2
Parent gene	AT5G19550
Parent gene annotation	Aspartate aminotransferase
Parent gene strand	+
Alternative splicing	AT5G19550_circ_g.2 AT5G19550_circ_g.4 AT5G19550_circ_g.5 AT5G19550_circ_g.6 AT5G19550_circ_g.7 AT5G19550_circ_g.8 AT5G19550_circ_g.9 AT5G19550_circ_g.10 AT5G19550_circ_g.11 AT5G19550_circ_g.12 AT5G19550_circ_g.13
Support reads	5/4
Tissues	leaf/whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G19550.1:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CCAACTTGGGGGAACCATCCCAAAGTTTTCAACTTGGCAGGCTTGTCTGTGGAGTATTTCCGCT
ACTATGATCCTGCAACCCGTGGTCTTGACTTCAAAGgtaactgttgcttacaacaatgatccta
gtcctgttaagatcaatttgggtgtcggtgcctaccgaactgaggtaatttttcgatttctgct
taaactgt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 439
Transcript exons: 6598597-6598668,6598783-6598844,6598939-6599022,6599148-
6599247,6599343-6599463
GTAACTGTTGCTTACAACAATGATCCTAGTCCTGTTAAGATCAATTTGGGTGTCGGTGCCTACC
GAACTGAGGAAGGGAAGCCTCTTGTTCTTGATGTGGTGCGTAAGGCAGAGCAACAGCTAGTGAA
CGATCCGTCTCGGGTCAAGGAATACATTCCCATTGTTGGTATTTCCGATTTTAACAAGTTAAGC
GCCAAGCTCATCTTAGGTGCTGACAGTCCTGCGATTACAGAAAGTAGAGTTACTACAGTCCAGT
GCTTGTCTGGTACTGGTTCGTTGAGAGTTGGGGCTGAGTTTCTCAAAACACACTACCACCAAAG
TGTCATTTACATTCCAAAACCAACTTGGGGGAACCATCCCAAAGTTTTCAACTTGGCAGGCTTG
TCTGTGGAGTATTTCCGCTACTATGATCCTGCAACCCGTGGTCTTGACTTCAAAG
Transcript length: 778
Transcript exons: 6598597-6599463
GTAACTGTTGCTTACAACAATGATCCTAGTCCTGTTAAGATCAATTTGGGTGTCGGTGCCTACC
GAACTGAGGAAGGGAAGCCTCTTGTTCTTGATGTGGTGCGTAAGGCAGAGCAACAGCTAGTGAA
CGATCCGTCTCGGGTCAAGGAATACATTCCCATTGTTGGTATTTCCGATTTTAACAAGTTAAGC
GCCAAGCTCATCTTAGGTGCTGACAGAGTGTCATTTACATTCCAAAACCAACTTGGGGGAACCA
TCCCAAAGTTTTCAACTTGGCAGGCTTGTCTGTGGAGTATTTCCGCTACTATGATCCTGCAACC
CGTGGTCTTGACTTCAAAGGTAACTGTTGCTTACAACAATGATCCTAGTCCTGTTAAGATCAAT
TTGGGTGTCGGTGCCTACCGAACTGAGGAAGGGAAGCCTCTTGTTCTTGATGTGGTGCGTAAGG
CAGAGCAACAGCTAGTGAACGATCCGTCTCGGGTCAAGGAATACATTCCCATTGTTGGTATTTC
CGATTTTAACAAGTTAAGCGCCAAGCTCATCTTAGGTGCTGACAGTCCTGCGATTACAGAAAGT
AGAGTTACTACAGTCCAGTGCTTGTCTGGTACTGGTTCGTTGAGAGTTGGGGCTGAGTTTCTCA
AAACACACTACCACCAAAGTGTCATTTACATTCCAAAACCAACTTGGGGGAACCATCCCAAAGT
TTTCAACTTGGCAGGCTTGTCTGTGGAGTATTTCCGCTACTATGATCCTGCAACCCGTGGTCTT
GACTTCAAAG
						
					

						Genomic sequence
						
							GTAACTGTTGCTTACAACAATGATCCTAGTCCTGTTAAGATCAATTTGGGTGTCGGTGCCTACC
GAACTGAGGTAATTTTTCGATTTCTGCTTAAACTGTTAAGCAACTTTATGGTATCAATTGTTTC
TACTAGCCAGTTTTTGAAAGCTCAGGTTTTGATTAGTTTGTTTCTCTTTTTTTCAAAGGAAGGG
AAGCCTCTTGTTCTTGATGTGGTGCGTAAGGCAGAGCAACAGCTAGTGAACGATCCGTGAGTTG
AAGAACATTGTCACTAATTCGTGTAGTTGTCTCTTCTTATTCTTGTTTTTTGCTAATGCCTAAA
TTCTTTTCTCTACTTCTCTAAGGTCTCGGGTCAAGGAATACATTCCCATTGTTGGTATTTCCGA
TTTTAACAAGTTAAGCGCCAAGCTCATCTTAGGTGCTGACAGGTAATCATCTATACTGTTTATT
TGTTATCTTTTCATTTGTGTTTTGCTTTGTTTCTCTTGTGACATAATATCCCCTTTAGTTGATA
AAGTTTAATATTTTATAAATATTCTTGTTTTGTTGGCAGTCCTGCGATTACAGAAAGTAGAGTT
ACTACAGTCCAGTGCTTGTCTGGTACTGGTTCGTTGAGAGTTGGGGCTGAGTTTCTCAAAACAC
ACTACCACCAAGTAAACTTCTCTCTATTTAGTTTTTTATTATATTTGACAGTCATTGAATTATG
TAATCAAACAAGTGTTGCTTACTATGGTTCTCATCCTTCCAGAGTGTCATTTACATTCCAAAAC
CAACTTGGGGGAACCATCCCAAAGTTTTCAACTTGGCAGGCTTGTCTGTGGAGTATTTCCGCTA
CTATGATCCTGCAACCCGTGGTCTTGACTTCAAAG
						
					

			
		

Sequences
Splice junction sequence	CCAACTTGGGGGAACCATCCCAAAGTTTTCAACTTGGCAGGCTTGTCTGTGGAGTATTTCCGCT ACTATGATCCTGCAACCCGTGGTCTTGACTTCAAAGgtaactgttgcttacaacaatgatccta gtcctgttaagatcaatttgggtgtcggtgcctaccgaactgaggtaatttttcgatttctgct taaactgt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 439 Transcript exons: 6598597-6598668,6598783-6598844,6598939-6599022,6599148- 6599247,6599343-6599463 GTAACTGTTGCTTACAACAATGATCCTAGTCCTGTTAAGATCAATTTGGGTGTCGGTGCCTACC GAACTGAGGAAGGGAAGCCTCTTGTTCTTGATGTGGTGCGTAAGGCAGAGCAACAGCTAGTGAA CGATCCGTCTCGGGTCAAGGAATACATTCCCATTGTTGGTATTTCCGATTTTAACAAGTTAAGC GCCAAGCTCATCTTAGGTGCTGACAGTCCTGCGATTACAGAAAGTAGAGTTACTACAGTCCAGT GCTTGTCTGGTACTGGTTCGTTGAGAGTTGGGGCTGAGTTTCTCAAAACACACTACCACCAAAG TGTCATTTACATTCCAAAACCAACTTGGGGGAACCATCCCAAAGTTTTCAACTTGGCAGGCTTG TCTGTGGAGTATTTCCGCTACTATGATCCTGCAACCCGTGGTCTTGACTTCAAAG Transcript length: 778 Transcript exons: 6598597-6599463 GTAACTGTTGCTTACAACAATGATCCTAGTCCTGTTAAGATCAATTTGGGTGTCGGTGCCTACC GAACTGAGGAAGGGAAGCCTCTTGTTCTTGATGTGGTGCGTAAGGCAGAGCAACAGCTAGTGAA CGATCCGTCTCGGGTCAAGGAATACATTCCCATTGTTGGTATTTCCGATTTTAACAAGTTAAGC GCCAAGCTCATCTTAGGTGCTGACAGAGTGTCATTTACATTCCAAAACCAACTTGGGGGAACCA TCCCAAAGTTTTCAACTTGGCAGGCTTGTCTGTGGAGTATTTCCGCTACTATGATCCTGCAACC CGTGGTCTTGACTTCAAAGGTAACTGTTGCTTACAACAATGATCCTAGTCCTGTTAAGATCAAT TTGGGTGTCGGTGCCTACCGAACTGAGGAAGGGAAGCCTCTTGTTCTTGATGTGGTGCGTAAGG CAGAGCAACAGCTAGTGAACGATCCGTCTCGGGTCAAGGAATACATTCCCATTGTTGGTATTTC CGATTTTAACAAGTTAAGCGCCAAGCTCATCTTAGGTGCTGACAGTCCTGCGATTACAGAAAGT AGAGTTACTACAGTCCAGTGCTTGTCTGGTACTGGTTCGTTGAGAGTTGGGGCTGAGTTTCTCA AAACACACTACCACCAAAGTGTCATTTACATTCCAAAACCAACTTGGGGGAACCATCCCAAAGT TTTCAACTTGGCAGGCTTGTCTGTGGAGTATTTCCGCTACTATGATCCTGCAACCCGTGGTCTT GACTTCAAAG
Genomic sequence	GTAACTGTTGCTTACAACAATGATCCTAGTCCTGTTAAGATCAATTTGGGTGTCGGTGCCTACC GAACTGAGGTAATTTTTCGATTTCTGCTTAAACTGTTAAGCAACTTTATGGTATCAATTGTTTC TACTAGCCAGTTTTTGAAAGCTCAGGTTTTGATTAGTTTGTTTCTCTTTTTTTCAAAGGAAGGG AAGCCTCTTGTTCTTGATGTGGTGCGTAAGGCAGAGCAACAGCTAGTGAACGATCCGTGAGTTG AAGAACATTGTCACTAATTCGTGTAGTTGTCTCTTCTTATTCTTGTTTTTTGCTAATGCCTAAA TTCTTTTCTCTACTTCTCTAAGGTCTCGGGTCAAGGAATACATTCCCATTGTTGGTATTTCCGA TTTTAACAAGTTAAGCGCCAAGCTCATCTTAGGTGCTGACAGGTAATCATCTATACTGTTTATT TGTTATCTTTTCATTTGTGTTTTGCTTTGTTTCTCTTGTGACATAATATCCCCTTTAGTTGATA AAGTTTAATATTTTATAAATATTCTTGTTTTGTTGGCAGTCCTGCGATTACAGAAAGTAGAGTT ACTACAGTCCAGTGCTTGTCTGGTACTGGTTCGTTGAGAGTTGGGGCTGAGTTTCTCAAAACAC ACTACCACCAAGTAAACTTCTCTCTATTTAGTTTTTTATTATATTTGACAGTCATTGAATTATG TAATCAAACAAGTGTTGCTTACTATGGTTCTCATCCTTCCAGAGTGTCATTTACATTCCAAAAC CAACTTGGGGGAACCATCCCAAAGTTTTCAACTTGGCAGGCTTGTCTGTGGAGTATTTCCGCTA CTATGATCCTGCAACCCGTGGTCTTGACTTCAAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.472166874

Conservation Information
Conserved circRNAs	NA
PMCS	0.472166874

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						response to drought stress

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	response to drought stress

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017;Liu et al., 2017;Zhang et al., 2019

circRNA

Detail information of AT5G19550_circ_g.3

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