Plant Circular RNA Database

Detail information of Zm00001d050150_circ_g.6

			
						 General Information
					
						CircRNA Name
						Zm00001d050150_circ_g.6
					
						ID in PlantcircBase
						zma_circ_008053
					
						Alias
						Zm04circ00038, zma_circ_0001450, GRMZM2G030628_C1
					
						Organism
						
							Zea mays						
					
						Position
						
							chr4: 69416932-69417887  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRI2, find_circ
					
						Parent gene
						
							Zm00001d050150						
					
						Parent gene annotation
						
							Adenylate kinase 5 chloroplastic
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Zm00001d050150_circ_g.1 Zm00001d050150_circ_g.2 Zm00001d050150_circ_g.3 Zm00001d050150_circ_g.4 Zm00001d050150_circ_g.5 Zm00001d050150_circ_g.7 Zm00001d050150_circ_g.8 Zm00001d050150_circ_g.9 						
					
						Support reads
						NA
					
						Tissues
						leaf, endosperm, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d050150_T002:5
Zm00001d050150_T003:5
Zm00001d050150_T001:5

General Information
CircRNA Name	Zm00001d050150_circ_g.6
ID in PlantcircBase	zma_circ_008053
Alias	Zm04circ00038, zma_circ_0001450, GRMZM2G030628_C1
Organism	Zea mays
Position	chr4: 69416932-69417887 JBrowse»
Reference genome	AGPv4.38
Type	ue-circRNA
Identification method	CIRI2, find_circ
Parent gene	Zm00001d050150
Parent gene annotation	Adenylate kinase 5 chloroplastic
Parent gene strand	+
Alternative splicing	Zm00001d050150_circ_g.1 Zm00001d050150_circ_g.2 Zm00001d050150_circ_g.3 Zm00001d050150_circ_g.4 Zm00001d050150_circ_g.5 Zm00001d050150_circ_g.7 Zm00001d050150_circ_g.8 Zm00001d050150_circ_g.9
Support reads	NA
Tissues	leaf, endosperm, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d050150_T002:5 Zm00001d050150_T003:5 Zm00001d050150_T001:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GGCATCCCACTTCAACTGGCAGGAACCAGAAAAATTTTGGAATTCATGGACTGGGGTGACTATG
GGGCGAAGGGCACCTTCATTAATATTGGAGCAGTAGgaaaacgaccagattctgcggatacagg
ttcaaaaaatgtatgcacaagatatactttagtttcatcttgttgacttgtgcaatattttgta
tcatgaac

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 420
Transcript exons: 69416932-69416969,69417084-69417209,69417443-69417478,69
417572-69417652,69417749-69417887
GAAAACGACCAGATTCTGCGGATACAGGTTCAAAAAATGAGTGGCGTGGAATTCCAACGAGGTT
GAATAACATTCCACATTCTAGGGAAATTAGGGAATACTTCTACAACGACGTGTTAAAAGCTACA
AAACTTGCTATTGAAGATAAAAAGACTCGGTTGCAGATAGATATCAATATTCCTGAACTTAACC
CTGAAATGGATGTTTACCGCATAGGAACCCTCATGGAACTTGTAAGAGAACTTTCTCTATCCTT
TGCAGATGATGGGAAACGTGTCAAGGTCTGTGTTCAAGGCTCCATGGGGCAAGGTGCATTTGCT
GGCATCCCACTTCAACTGGCAGGAACCAGAAAAATTTTGGAATTCATGGACTGGGGTGACTATG
GGGCGAAGGGCACCTTCATTAATATTGGAGCAGTAG
						
						Genomic sequence
						
							GAAAACGACCAGATTCTGCGGATACAGGTTCAAAAAATGTATGCACAAGATATACTTTAGTTTC
ATCTTGTTGACTTGTGCAATATTTTGTATCATGAACTACAAAGATTTGGATCTGTATGGTTCAT
ATGTGTTTTGCGGTTGTAAACCAGGAGTGGCGTGGAATTCCAACGAGGTTGAATAACATTCCAC
ATTCTAGGGAAATTAGGGAATACTTCTACAACGACGTGTTAAAAGCTACAAAACTTGCTATTGA
AGATAAAAAGACTCGGTTGCAGGTTGCTTCTTATCTTTTCCATTTATGTTAATTTGTTTTGTAA
GATTGAAATCACAGTATTGTGTTATTTATTCTGTTTCTGTTGCATAGATAGTCGAGTACTGGAA
TATGTGAGCTTCATGTGATTAATTCACGTAACTGAACAATCTAATTCTTATTTCCAGTTTGGCT
ACCTATTTTTTGAGAATAAACTGACATATGCTGATCTATGTCCTCATTTTCCCAAATATGCAGA
TAGATATCAATATTCCTGAACTTAACCCTGAAATGGTAAGTGTACATATGGTTACACATATGTA
TTTTGTAATGTCTGTTAGAAAACACTGCTGATTTTAACTGCAACCAAATTCGTTTTATTGCCAG
GATGTTTACCGCATAGGAACCCTCATGGAACTTGTAAGAGAACTTTCTCTATCCTTTGCAGATG
ATGGGAAACGTGTCAAGGTGATCTTTGCAACAGATGCATATACTATTGCTCAGCCGCACCCTTC
TTTGTCCTCCCCTAGTAACAAAATTATGTGACTAGCCACTTGATACCAGGTCTGTGTTCAAGGC
TCCATGGGGCAAGGTGCATTTGCTGGCATCCCACTTCAACTGGCAGGAACCAGAAAAATTTTGG
AATTCATGGACTGGGGTGACTATGGGGCGAAGGGCACCTTCATTAATATTGGAGCAGTAG

Sequences
Splice junction sequence	GGCATCCCACTTCAACTGGCAGGAACCAGAAAAATTTTGGAATTCATGGACTGGGGTGACTATG GGGCGAAGGGCACCTTCATTAATATTGGAGCAGTAGgaaaacgaccagattctgcggatacagg ttcaaaaaatgtatgcacaagatatactttagtttcatcttgttgacttgtgcaatattttgta tcatgaac
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 420 Transcript exons: 69416932-69416969,69417084-69417209,69417443-69417478,69 417572-69417652,69417749-69417887 GAAAACGACCAGATTCTGCGGATACAGGTTCAAAAAATGAGTGGCGTGGAATTCCAACGAGGTT GAATAACATTCCACATTCTAGGGAAATTAGGGAATACTTCTACAACGACGTGTTAAAAGCTACA AAACTTGCTATTGAAGATAAAAAGACTCGGTTGCAGATAGATATCAATATTCCTGAACTTAACC CTGAAATGGATGTTTACCGCATAGGAACCCTCATGGAACTTGTAAGAGAACTTTCTCTATCCTT TGCAGATGATGGGAAACGTGTCAAGGTCTGTGTTCAAGGCTCCATGGGGCAAGGTGCATTTGCT GGCATCCCACTTCAACTGGCAGGAACCAGAAAAATTTTGGAATTCATGGACTGGGGTGACTATG GGGCGAAGGGCACCTTCATTAATATTGGAGCAGTAG
Genomic sequence	GAAAACGACCAGATTCTGCGGATACAGGTTCAAAAAATGTATGCACAAGATATACTTTAGTTTC ATCTTGTTGACTTGTGCAATATTTTGTATCATGAACTACAAAGATTTGGATCTGTATGGTTCAT ATGTGTTTTGCGGTTGTAAACCAGGAGTGGCGTGGAATTCCAACGAGGTTGAATAACATTCCAC ATTCTAGGGAAATTAGGGAATACTTCTACAACGACGTGTTAAAAGCTACAAAACTTGCTATTGA AGATAAAAAGACTCGGTTGCAGGTTGCTTCTTATCTTTTCCATTTATGTTAATTTGTTTTGTAA GATTGAAATCACAGTATTGTGTTATTTATTCTGTTTCTGTTGCATAGATAGTCGAGTACTGGAA TATGTGAGCTTCATGTGATTAATTCACGTAACTGAACAATCTAATTCTTATTTCCAGTTTGGCT ACCTATTTTTTGAGAATAAACTGACATATGCTGATCTATGTCCTCATTTTCCCAAATATGCAGA TAGATATCAATATTCCTGAACTTAACCCTGAAATGGTAAGTGTACATATGGTTACACATATGTA TTTTGTAATGTCTGTTAGAAAACACTGCTGATTTTAACTGCAACCAAATTCGTTTTATTGCCAG GATGTTTACCGCATAGGAACCCTCATGGAACTTGTAAGAGAACTTTCTCTATCCTTTGCAGATG ATGGGAAACGTGTCAAGGTGATCTTTGCAACAGATGCATATACTATTGCTCAGCCGCACCCTTC TTTGTCCTCCCCTAGTAACAAAATTATGTGACTAGCCACTTGATACCAGGTCTGTGTTCAAGGC TCCATGGGGCAAGGTGCATTTGCTGGCATCCCACTTCAACTGGCAGGAACCAGAAAAATTTTGG AATTCATGGACTGGGGTGACTATGGGGCGAAGGGCACCTTCATTAATATTGGAGCAGTAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.142317018

Conservation Information
Conserved circRNAs	NA
PMCS	0.142317018

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							69417477-69417884(+)
69417120-69417849(-)
						
						Potential amino acid sequence
						
							MDVYRIGTLMELVRELSLSFADDGKRVKVCVQGSMGQGAFAGIPLQLAGTRKILEFMDWGDYGA
KGTFINIGAVGKRPDSADTGSKNEWRGIPTRLNNIPHSREIREYFYNDVLKATKLAIEDKKTRL
QIDINIPELNPEMDVYRIGTLMELVRELSLSFADDGKRVKVCVQGSMGQGAFAGIPLQLAGTRK
ILEFMDWGDYGAKGTFINIGAVGKRPDSADTGSKNEWRGIPTRLNNIPHSREIREYFYNDVLKA
TKLAIEDKKTRLQIDINIPELNPEMDVYRIGTLMELVRELSLSFADDGKRVKVCVQGSMGQGAF
AGIPLQLAGTRKILEFMDWGDYGAKGTFINIGAVGKRPDSADTGSKNEWRGIPTRLNNIPHSRE
IREYFYNDVLKATKLAIEDKKTRLQIDINIPELNPEMDVYRIGTLMELVRELSLSFADDGKRVK
VCVQGSMGQGAFAGIPLQLAGTRKILEFMDWGDYGAKGTFINIGAV(+)
MLFNLVGIPRHSFFEPVSAESGRFPTAPILMKVPFAP*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to drought stress

Functional Information
Coding potential	Y
Potential coding position	69417477-69417884(+) 69417120-69417849(-)
Potential amino acid sequence	MDVYRIGTLMELVRELSLSFADDGKRVKVCVQGSMGQGAFAGIPLQLAGTRKILEFMDWGDYGA KGTFINIGAVGKRPDSADTGSKNEWRGIPTRLNNIPHSREIREYFYNDVLKATKLAIEDKKTRL QIDINIPELNPEMDVYRIGTLMELVRELSLSFADDGKRVKVCVQGSMGQGAFAGIPLQLAGTRK ILEFMDWGDYGAKGTFINIGAVGKRPDSADTGSKNEWRGIPTRLNNIPHSREIREYFYNDVLKA TKLAIEDKKTRLQIDINIPELNPEMDVYRIGTLMELVRELSLSFADDGKRVKVCVQGSMGQGAF AGIPLQLAGTRKILEFMDWGDYGAKGTFINIGAVGKRPDSADTGSKNEWRGIPTRLNNIPHSRE IREYFYNDVLKATKLAIEDKKTRLQIDINIPELNPEMDVYRIGTLMELVRELSLSFADDGKRVK VCVQGSMGQGAFAGIPLQLAGTRKILEFMDWGDYGAKGTFINIGAV(+) MLFNLVGIPRHSFFEPVSAESGRFPTAPILMKVPFAP*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to drought stress

			
						 Other Information
					
						References
						Han et al., 2020; Ma et al., 2021b;Zhang et al., 2019

circRNA

Detail information of Zm00001d050150_circ_g.6

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