Plant Circular RNA Database

Detail information of BGIOSGA016665_circ_g.5

			
						 General Information
					
						CircRNA Name
						BGIOSGA016665_circ_g.5
					
						ID in PlantcircBase
						osi_circ_005578
					
						Alias
						4:23072596|23075451
					
						Organism
						
							Oryza sativa ssp. indica						
					
						Position
						
							chr4: 23072596-23075451  JBrowse»						
					
						Reference genome
						
							Oryza_indica.ASM465v1.42						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							BGIOSGA016665						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							BGIOSGA016665_circ_g.3 BGIOSGA016665_circ_g.4 BGIOSGA016665_circ_g.6 						
					
						Support reads
						NA
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						BGIOSGA016665-TA:6

General Information
CircRNA Name	BGIOSGA016665_circ_g.5
ID in PlantcircBase	osi_circ_005578
Alias	4:23072596\|23075451
Organism	Oryza sativa ssp. indica
Position	chr4: 23072596-23075451 JBrowse»
Reference genome	Oryza_indica.ASM465v1.42
Type	e-circRNA
Identification method	find_circ
Parent gene	BGIOSGA016665
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	BGIOSGA016665_circ_g.3 BGIOSGA016665_circ_g.4 BGIOSGA016665_circ_g.6
Support reads	NA
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	BGIOSGA016665-TA:6

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GATAAGCACACCTATTATCACTGCTGAGTTACTTTCTGAGAAGGATGTGTTGTCTGCACGCATT
GTGAAAGGCTCTATGGAAAAGGCAGTGCTGGGTGAGgtctgtatgaatacacttcactggagga
acagcccattgatcatgtctcagtgtggatctaaaggttctccaatcaacattagtcagatggt
tgcatgtg

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							GTCTGTATGAATACACTTCACTGGAGGAACAGCCCATTGATCATGTCTCAGTGTGGATCTAAAG
GTTCTCCAATCAACATTAGTCAGATGGTTGCATGTGTTGGCCAACAATCAGTTGGAGGGCGTCG
TGCCCCGAATGGCTTCATAGATCGAACTCTTCCCCACTTTCCTATAAATTCAAAGACTCCTGCA
GTAAGAACATCAGTACTAGAAATATGACTTGCTTATTTGTGTATCCATTTCTGGTGTTGGATAT
TTCTAGTTCTGTTCAAATAAGTACTCTTCAAATTTTATGGGGCACTATACATAGAGATAGAGGC
TGGAAATTGAAAATATATGTAATGTTTTATTTTAAACACAATATCTCTTATGTTATAGAAAGTT
CTGAATTGACATTACTTCCCTTTGATGTATATATATGTGGTATCCTAAATGGCTAATTAATTAT
TTATTTTTCATTAAAGGATCGTGGGATTCTATTGGAAGTTATCATGTAGAAAAATTGGTCCTTT
TCAGAGTGGGGATTTCATTGACTCGTAGTCCTCCACCTAGCTTTGTTAGAGAAAACATTTTTTT
ATTCAACTTGTTCTGACTGCAATTTTGTTTGAATTAAATGTCACGAAATCAGCACATTTTGGTA
TATTCACATACAAATATGTTTTGTATTCCATTTCCAGGCCAAAGGCTTTGTCGCTAATTCTTTC
TATACTGGCTTGACTGCCACCGAATTCTTCTTCCATACGATGGGTGGGCGAGAAGGTCTTGTTG
ATACAGCGGTATGTCTATGTATAAAACCATCTATTGTTTGCATAAGTTGATATTTGCACTCTAT
CTGTATTTCAGCACAAAAATTTCTTAAATTTGCTCACATAGACTTACACCGCAGTACATACTAC
TCAATTGAATTAATAGTAGTACAATGATGATATTATGTGAGAGGAATAGTCTGAATTAGCTCCT
TGTGCCTGGCAGGGATAGGATTAGGAAACTTAGCAGCTATTATAGAAGAGCACATCATCAACTA
GAAACAAATGATAAGTTCATTCATCACATTCTTATAGAATTAAGCCCACTTCTCCTTTATATTT
TGAGCACAGCAATGATTGGGGAAGATGTTTCCTTGTTCGTTGTTTGGGCACTAAATCAACTTGC
TTGCATACCAGAGTTGAATCTGGCGTTTCCGTTTGACTCTGCCTTTTCGGCTTTGGTCCTTGCA
GTTGAGTGTTTGATCTCAGGCACTGTAGTTCGCTGCATGAAAACCTTCACAGCACTATGCCAGT
TCTATGTAGAGGTCTGTTAGGTGAACTATACTGGTATCTTGTGCATTCAGCTGGACCAAGGTGC
AACCCATTGGGCATTTCCAGCATACTCATTGTGCCTTCTGGCTTCTCAAAAACATTTGGGAATT
GTTGGAAATTTGGAATATAATTTCTGCTTGTTTCCTTGGTTGTGAACTTGTGATCGTAGGTTAA
AACAGCTGAAACAGGATATATGTCCCGCAGATTAATGAAGGGATTGGAAGATCTCTCAGTTTTC
TATGATCAGACGGTCCGCAATGCTAGTGGTGGTATTGTACAGTTCCTCTATGGAGATGATGGCA
TGGATCCTGCAAAGATGGAAGGGAAAGATGGAAAGCCCTTAAATTTGGATCAATTGTTTATGAA
AGTCATGGTCAGTTTTAACATGTAAAAACAGTTTGTTTACTTGATCAAGTGAGATGCGCCTCGT
GTGCTAACCATTTTACTTTTGTAGGCCACTTGTCCTCAAAGGGGACAGAATACATTATCTCCAG
GTGAAATCTTACAAATTTTAAATGACAAGCTCTCTGAACATGATGCCTCCTCTGATGATGGTTG
TAGCGAAAAGTTCAAGCAACTGTTAACGTATTTTCTTGAGGATCGTATAAAATTGCTGAAGAGC
ACAAGGAGAGCGCTTCTCCTAGATGAAGATCATGTTGGAGAGAGGCACTCCAGCTTTGAAGAAT
CCATTGCTGCTAATATTTCTGGTATATCTGTGAAGCAACTACAGGTGTGTCTATTCATATTTTT
CACATACCAATTCATCTCTGTTTTGCGAAAAACCTGAAATATCAAGCACAGTCTATTTCTGATC
AATTTTTTGGTCTCTTACTAATTGCGTGGTCATGCTAATTTTCTGCAGTAAATGTTAAACTGAT
TTCAAGTATTATTTTTATTGGTTTGCTTATGCATTTTATCCTTGTGTCGTCATTCTGTAATGGT
TTTCCTTTTCTCTATATGGCTCAGGTTTTCTTGGACACATGTTTATCTCGTTACCACTTGAAGA
AAATTGAAGCCGGAGCATCAATTGGGGCAATTGGGGCTCAAAGTATTGGAGAGCCTGGAACTCA
GATGACATTGAAAACTTTCCATTTTGCAGGAGTAGCTAGCATGAGTATCCTATATACTATATGG
TTCGTGAAAGTCTGCTTTTAATTTTAAGAATTACTAGCAGGTCGTTTTGGCCTATTTCCTATAA
TTTATTTACTTTAGAGGGGCACTGGAATGTAGTATCTAAGATAAAGCGGATGAAATTGTCAAGT
AATATGCGCTAGAAGATATAAGCTTTCATCAATCTGAATTGATTTGCACAATACTACGGCATTT
GATATCTTGATGTAGAGTAATGCATACATGCTATTCTTGGACACTGCTGTTCAAATCATCTTTC
CTTAACAAGTAAAAAGATGTTACTCTTGGTGTTCCTCGCATCAAGGAAATTATCAATGCTGCTA
AAAAGATAAGCACACCTATTATCACTGCTGAGTTACTTTCTGAGAAGGATGTGTTGTCTGCACG
CATTGTGAAAGGCTCTATGGAAAAGGCAGTGCTGGGTGAG
						
					

			
		

Sequences
Splice junction sequence	GATAAGCACACCTATTATCACTGCTGAGTTACTTTCTGAGAAGGATGTGTTGTCTGCACGCATT GTGAAAGGCTCTATGGAAAAGGCAGTGCTGGGTGAGgtctgtatgaatacacttcactggagga acagcccattgatcatgtctcagtgtggatctaaaggttctccaatcaacattagtcagatggt tgcatgtg
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GTCTGTATGAATACACTTCACTGGAGGAACAGCCCATTGATCATGTCTCAGTGTGGATCTAAAG GTTCTCCAATCAACATTAGTCAGATGGTTGCATGTGTTGGCCAACAATCAGTTGGAGGGCGTCG TGCCCCGAATGGCTTCATAGATCGAACTCTTCCCCACTTTCCTATAAATTCAAAGACTCCTGCA GTAAGAACATCAGTACTAGAAATATGACTTGCTTATTTGTGTATCCATTTCTGGTGTTGGATAT TTCTAGTTCTGTTCAAATAAGTACTCTTCAAATTTTATGGGGCACTATACATAGAGATAGAGGC TGGAAATTGAAAATATATGTAATGTTTTATTTTAAACACAATATCTCTTATGTTATAGAAAGTT CTGAATTGACATTACTTCCCTTTGATGTATATATATGTGGTATCCTAAATGGCTAATTAATTAT TTATTTTTCATTAAAGGATCGTGGGATTCTATTGGAAGTTATCATGTAGAAAAATTGGTCCTTT TCAGAGTGGGGATTTCATTGACTCGTAGTCCTCCACCTAGCTTTGTTAGAGAAAACATTTTTTT ATTCAACTTGTTCTGACTGCAATTTTGTTTGAATTAAATGTCACGAAATCAGCACATTTTGGTA TATTCACATACAAATATGTTTTGTATTCCATTTCCAGGCCAAAGGCTTTGTCGCTAATTCTTTC TATACTGGCTTGACTGCCACCGAATTCTTCTTCCATACGATGGGTGGGCGAGAAGGTCTTGTTG ATACAGCGGTATGTCTATGTATAAAACCATCTATTGTTTGCATAAGTTGATATTTGCACTCTAT CTGTATTTCAGCACAAAAATTTCTTAAATTTGCTCACATAGACTTACACCGCAGTACATACTAC TCAATTGAATTAATAGTAGTACAATGATGATATTATGTGAGAGGAATAGTCTGAATTAGCTCCT TGTGCCTGGCAGGGATAGGATTAGGAAACTTAGCAGCTATTATAGAAGAGCACATCATCAACTA GAAACAAATGATAAGTTCATTCATCACATTCTTATAGAATTAAGCCCACTTCTCCTTTATATTT TGAGCACAGCAATGATTGGGGAAGATGTTTCCTTGTTCGTTGTTTGGGCACTAAATCAACTTGC TTGCATACCAGAGTTGAATCTGGCGTTTCCGTTTGACTCTGCCTTTTCGGCTTTGGTCCTTGCA GTTGAGTGTTTGATCTCAGGCACTGTAGTTCGCTGCATGAAAACCTTCACAGCACTATGCCAGT TCTATGTAGAGGTCTGTTAGGTGAACTATACTGGTATCTTGTGCATTCAGCTGGACCAAGGTGC AACCCATTGGGCATTTCCAGCATACTCATTGTGCCTTCTGGCTTCTCAAAAACATTTGGGAATT GTTGGAAATTTGGAATATAATTTCTGCTTGTTTCCTTGGTTGTGAACTTGTGATCGTAGGTTAA AACAGCTGAAACAGGATATATGTCCCGCAGATTAATGAAGGGATTGGAAGATCTCTCAGTTTTC TATGATCAGACGGTCCGCAATGCTAGTGGTGGTATTGTACAGTTCCTCTATGGAGATGATGGCA TGGATCCTGCAAAGATGGAAGGGAAAGATGGAAAGCCCTTAAATTTGGATCAATTGTTTATGAA AGTCATGGTCAGTTTTAACATGTAAAAACAGTTTGTTTACTTGATCAAGTGAGATGCGCCTCGT GTGCTAACCATTTTACTTTTGTAGGCCACTTGTCCTCAAAGGGGACAGAATACATTATCTCCAG GTGAAATCTTACAAATTTTAAATGACAAGCTCTCTGAACATGATGCCTCCTCTGATGATGGTTG TAGCGAAAAGTTCAAGCAACTGTTAACGTATTTTCTTGAGGATCGTATAAAATTGCTGAAGAGC ACAAGGAGAGCGCTTCTCCTAGATGAAGATCATGTTGGAGAGAGGCACTCCAGCTTTGAAGAAT CCATTGCTGCTAATATTTCTGGTATATCTGTGAAGCAACTACAGGTGTGTCTATTCATATTTTT CACATACCAATTCATCTCTGTTTTGCGAAAAACCTGAAATATCAAGCACAGTCTATTTCTGATC AATTTTTTGGTCTCTTACTAATTGCGTGGTCATGCTAATTTTCTGCAGTAAATGTTAAACTGAT TTCAAGTATTATTTTTATTGGTTTGCTTATGCATTTTATCCTTGTGTCGTCATTCTGTAATGGT TTTCCTTTTCTCTATATGGCTCAGGTTTTCTTGGACACATGTTTATCTCGTTACCACTTGAAGA AAATTGAAGCCGGAGCATCAATTGGGGCAATTGGGGCTCAAAGTATTGGAGAGCCTGGAACTCA GATGACATTGAAAACTTTCCATTTTGCAGGAGTAGCTAGCATGAGTATCCTATATACTATATGG TTCGTGAAAGTCTGCTTTTAATTTTAAGAATTACTAGCAGGTCGTTTTGGCCTATTTCCTATAA TTTATTTACTTTAGAGGGGCACTGGAATGTAGTATCTAAGATAAAGCGGATGAAATTGTCAAGT AATATGCGCTAGAAGATATAAGCTTTCATCAATCTGAATTGATTTGCACAATACTACGGCATTT GATATCTTGATGTAGAGTAATGCATACATGCTATTCTTGGACACTGCTGTTCAAATCATCTTTC CTTAACAAGTAAAAAGATGTTACTCTTGGTGTTCCTCGCATCAAGGAAATTATCAATGCTGCTA AAAAGATAAGCACACCTATTATCACTGCTGAGTTACTTTCTGAGAAGGATGTGTTGTCTGCACG CATTGTGAAAGGCTCTATGGAAAAGGCAGTGCTGGGTGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS

Conservation Information
Conserved circRNAs	NA
PMCS

			
				
						 Functional Information
					

				
						Coding potential
						Y
					

						Potential coding position
						
							23072634-23075428(-)
23072603-23075448(+)
						
					

						Potential amino acid sequence
						
							MGCSSSEVYSYRPHPALPFP*(-)
MNTLHWRNSPLIMSQCGSKGSPINISQMVACVGQQSVGGRRAPNGFIDRTLPHFPINSKTPAAK
GFVANSFYTGLTATEFFFHTMGGREGLVDTAVKTAETGYMSRRLMKGLEDLSVFYDQTVRNASG
GIVQFLYGDDGMDPAKMEGKDGKPLNLDQLFMKVMATCPQRGQNTLSPGEILQILNDKLSEHDA
SSDDGCSEKFKQLLTYFLEDRIKLLKSTRRALLLDEDHVGERHSSFEESIAANISGISVKQLQV
FLDTCLSRYHLKKIEAGASIGAIGAQSIGEPGTQMTLKTFHFAGVASMNVTLGVPRIKEIINAA
KKISTPIITAELLSEKDVLSARIVKGSMEKAVLGEVCMNTLHWRNSPLIMSQCGSKGSPINISQ
MVACVGQQSVGGRRAPNGFIDRTLPHFPINSKTPAAKGFVANSFYTGLTATEFFFHTMGGREGL
VDTAVKTAETGYMSRRLMKGLEDLSVFYDQTVRNASGGIVQFLYGDDGMDPAKMEGKDGKPLNL
DQLFMKVMATCPQRGQNTLSPGEILQILNDKLSEHDASSDDGCSEKFKQLLTYFLEDRIKLLKS
TRRALLLDEDHVGERHSSFEESIAANISGISVKQLQVFLDTCLSRYHLKKIEAGASIGAIGAQS
IGEPGTQMTLKTFHFAGVASMNVTLGVPRIKEIINAAKKISTPIITAELLSEKDVLSARIVKGS
MEKAVLGEVCMNTLHWRNSPLIMSQCGSKGSPINISQMVACVGQQSVGGRRAPNGFIDRTLPHF
PINSKTPAAKGFVANSFYTGLTATEFFFHTMGGREGLVDTAVKTAETGYMSRRLMKGLEDLSVF
YDQTVRNASGGIVQFLYGDDGMDPAKMEGKDGKPLNLDQLFMKVMATCPQRGQNTLSPGEILQI
LNDKLSEHDASSDDGCSEKFKQLLTYFLEDRIKLLKSTRRALLLDEDHVGERHSSFEESIAANI
SGISVKQLQVFLDTCLSRYHLKKIEAGASIGAIGAQSIGEPGTQMTLKTFHFAGVASMNVTLGV
PRIKEIINAAKKISTPIITAELLSEKDVLSARIVKGSMEKAVLGEVCMNTLHWRNSPLIMSQCG
SKGSPINISQMVACVGQQSVGGRRAPNGFIDRTLPHFPINSKTPAAKGFVANSFYTGLTATEFF
FHTMGGREGLVDTAVKTAETGYMSRRLMKGLEDLSVFYDQTVRNASGGIVQFLYGDDGMDPAKM
EGKDGKPLNLDQLFMKVMATCPQRGQNTLSPGEILQILNDKLSEHDASSDDGCSEKFKQLLTYF
LEDRIKLLKSTRRALLLDEDHVGERHSSFEESIAANISGISVKQLQVFLDTCLSRYHLKKIEAG
ASIGAIGAQSIGEPGTQMTLKTFHFAGVASMNVTLGVPRIKEIINAAKKISTPIITAELLSEKD
VLSARIVKGSMEKAVLGE(+)
						
					

						Sponge-miRNAs
						
							NA						
					

						circRNA-miRNA-mRNA network
						 VISUALIZATION
					

						Potential function description
						NA
					

			
		

Functional Information
Coding potential	Y
Potential coding position	23072634-23075428(-) 23072603-23075448(+)
Potential amino acid sequence	MGCSSSEVYSYRPHPALPFP*(-) MNTLHWRNSPLIMSQCGSKGSPINISQMVACVGQQSVGGRRAPNGFIDRTLPHFPINSKTPAAK GFVANSFYTGLTATEFFFHTMGGREGLVDTAVKTAETGYMSRRLMKGLEDLSVFYDQTVRNASG GIVQFLYGDDGMDPAKMEGKDGKPLNLDQLFMKVMATCPQRGQNTLSPGEILQILNDKLSEHDA SSDDGCSEKFKQLLTYFLEDRIKLLKSTRRALLLDEDHVGERHSSFEESIAANISGISVKQLQV FLDTCLSRYHLKKIEAGASIGAIGAQSIGEPGTQMTLKTFHFAGVASMNVTLGVPRIKEIINAA KKISTPIITAELLSEKDVLSARIVKGSMEKAVLGEVCMNTLHWRNSPLIMSQCGSKGSPINISQ MVACVGQQSVGGRRAPNGFIDRTLPHFPINSKTPAAKGFVANSFYTGLTATEFFFHTMGGREGL VDTAVKTAETGYMSRRLMKGLEDLSVFYDQTVRNASGGIVQFLYGDDGMDPAKMEGKDGKPLNL DQLFMKVMATCPQRGQNTLSPGEILQILNDKLSEHDASSDDGCSEKFKQLLTYFLEDRIKLLKS TRRALLLDEDHVGERHSSFEESIAANISGISVKQLQVFLDTCLSRYHLKKIEAGASIGAIGAQS IGEPGTQMTLKTFHFAGVASMNVTLGVPRIKEIINAAKKISTPIITAELLSEKDVLSARIVKGS MEKAVLGEVCMNTLHWRNSPLIMSQCGSKGSPINISQMVACVGQQSVGGRRAPNGFIDRTLPHF PINSKTPAAKGFVANSFYTGLTATEFFFHTMGGREGLVDTAVKTAETGYMSRRLMKGLEDLSVF YDQTVRNASGGIVQFLYGDDGMDPAKMEGKDGKPLNLDQLFMKVMATCPQRGQNTLSPGEILQI LNDKLSEHDASSDDGCSEKFKQLLTYFLEDRIKLLKSTRRALLLDEDHVGERHSSFEESIAANI SGISVKQLQVFLDTCLSRYHLKKIEAGASIGAIGAQSIGEPGTQMTLKTFHFAGVASMNVTLGV PRIKEIINAAKKISTPIITAELLSEKDVLSARIVKGSMEKAVLGEVCMNTLHWRNSPLIMSQCG SKGSPINISQMVACVGQQSVGGRRAPNGFIDRTLPHFPINSKTPAAKGFVANSFYTGLTATEFF FHTMGGREGLVDTAVKTAETGYMSRRLMKGLEDLSVFYDQTVRNASGGIVQFLYGDDGMDPAKM EGKDGKPLNLDQLFMKVMATCPQRGQNTLSPGEILQILNDKLSEHDASSDDGCSEKFKQLLTYF LEDRIKLLKSTRRALLLDEDHVGERHSSFEESIAANISGISVKQLQVFLDTCLSRYHLKKIEAG ASIGAIGAQSIGEPGTQMTLKTFHFAGVASMNVTLGVPRIKEIINAAKKISTPIITAELLSEKD VLSARIVKGSMEKAVLGE(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Huang et al., 2021

circRNA

Detail information of BGIOSGA016665_circ_g.5

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