Plant Circular RNA Database

Detail information of Os01g0846000_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os01g0846000_circ_g.3
					
						ID in PlantcircBase
						osa_circ_004810
					
						Alias
						Os01circ27545
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 36327923-36328628  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer, SMALT, Segemehl
					
						Parent gene
						
							Os01g0846000						
					
						Parent gene annotation
						
							Nucleic acid-binding, OB-fold domain containing protein. (Os01t0
846000-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os01g0846000_circ_g.1 Os01g0846000_circ_g.2 						
					
						Support reads
						2/1
					
						Tissues
						leaf/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0846000-01:3

General Information
CircRNA Name	Os01g0846000_circ_g.3
ID in PlantcircBase	osa_circ_004810
Alias	Os01circ27545
Organism	Oryza sativa
Position	chr1: 36327923-36328628 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer, SMALT, Segemehl
Parent gene	Os01g0846000
Parent gene annotation	Nucleic acid-binding, OB-fold domain containing protein. (Os01t0 846000-01)
Parent gene strand	-
Alternative splicing	Os01g0846000_circ_g.1 Os01g0846000_circ_g.2
Support reads	2/1
Tissues	leaf/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0846000-01:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GTTCATTTGCTGTAGTGAACTGCGAGAAGCGCGTGACCCGCACTCGGACTTGCCAATGGTGTGA
TTCCTGTGTCACATTCCTTAGCATAGTGTAATCCATcttccattggttatgtggacattaagca
ccgctttcataacacctccacaagaaacagtttgcactggtccaatggctgtcattatacctat
agcatcta

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 514
Transcript exons: 36327923-36328019,36328135-36328407,36328485-36328628
CTTCCATTGGTTATGTGGACATTAAGCACCGCTTTCATAACACCTCCACAAGAAACAGTTTGCA
CTGGTCCAATGGCTGTCATTATACCTATAGCATCTGAAAGAATATCTTTCCTGTAAGCACGACC
TCTGAGTTCATCAAAAGAAAGAACCTTGTAAGCATGAGATGGGAATGTGGATGGTGCCTCAGTG
TCTTCTCTAACGGTTGTGTGGGCAGTGAATTTTGCCATGACCATCCGATCAATAGGCCTGTACT
GGGGGCGCGCCTCAGCAACTTCGAAGTACTTGATGTAATACACAGCATCCTCCTTCAGCAAAGG
CTTGAATTGAGAAACATGACGTCCTGGGATCTGCGCATCCATCATGTACCCTGCTCATCAAGCA
GGACTAAATCAAGTCGCAGAATCTTGTCAGGTTCATTTGCTGTAGTGAACTGCGAGAAGCGCGT
GACCCGCACTCGGACTTGCCAATGGTGTGATTCCTGTGTCACATTCCTTAGCATAGTGTAATCC
AT
						
						Genomic sequence
						
							CTTCCATTGGTTATGTGGACATTAAGCACCGCTTTCATAACACCTCCACAAGAAACAGTTTGCA
CTGGTCCAATGGCTGTCATTATACCTATAGCATCTACAGGAGTAGTATAAGGGATAAAATTTGC
ACATGAGTAAGTGAGAGCAGTAAGCAGGGAATGGACAAAGCTAATAAGGAATGAGATAGATTGA
TCTGGACCAAACAGAGTCACCTGAAAGAATATCTTTCCTGTAAGCACGACCTCTGAGTTCATCA
AAAGAAAGAACCTTGTAAGCATGAGATGGGAATGTGGATGGTGCCTCAGTGTCTTCTCTAACGG
TTGTGTGGGCAGTGAATTTTGCCATGACCATCCGATCAATAGGCCTGTACTGGGGGCGCGCCTC
AGCAACTTCGAAGTACTTGATGTAATACACAGCATCCTCCTTCAGCAAAGGCTTGAATTGAGAA
ACATGACGTCCTGGGATCTGCGCATCCATCATGTACCCTTGTTGATGTTTGGATGTGTAAGAGA
CAAATAGCATGGAGGTAAAATAAGGCATGACCATGTGCTTATTGCTATACCTGCTCATCAAGCA
GGACTAAATCAAGTCGCAGAATCTTGTCAGGTTCATTTGCTGTAGTGAACTGCGAGAAGCGCGT
GACCCGCACTCGGACTTGCCAATGGTGTGATTCCTGTGTCACATTCCTTAGCATAGTGTAATCC
AT

Sequences
Splice junction sequence	GTTCATTTGCTGTAGTGAACTGCGAGAAGCGCGTGACCCGCACTCGGACTTGCCAATGGTGTGA TTCCTGTGTCACATTCCTTAGCATAGTGTAATCCATcttccattggttatgtggacattaagca ccgctttcataacacctccacaagaaacagtttgcactggtccaatggctgtcattatacctat agcatcta
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 514 Transcript exons: 36327923-36328019,36328135-36328407,36328485-36328628 CTTCCATTGGTTATGTGGACATTAAGCACCGCTTTCATAACACCTCCACAAGAAACAGTTTGCA CTGGTCCAATGGCTGTCATTATACCTATAGCATCTGAAAGAATATCTTTCCTGTAAGCACGACC TCTGAGTTCATCAAAAGAAAGAACCTTGTAAGCATGAGATGGGAATGTGGATGGTGCCTCAGTG TCTTCTCTAACGGTTGTGTGGGCAGTGAATTTTGCCATGACCATCCGATCAATAGGCCTGTACT GGGGGCGCGCCTCAGCAACTTCGAAGTACTTGATGTAATACACAGCATCCTCCTTCAGCAAAGG CTTGAATTGAGAAACATGACGTCCTGGGATCTGCGCATCCATCATGTACCCTGCTCATCAAGCA GGACTAAATCAAGTCGCAGAATCTTGTCAGGTTCATTTGCTGTAGTGAACTGCGAGAAGCGCGT GACCCGCACTCGGACTTGCCAATGGTGTGATTCCTGTGTCACATTCCTTAGCATAGTGTAATCC AT
Genomic sequence	CTTCCATTGGTTATGTGGACATTAAGCACCGCTTTCATAACACCTCCACAAGAAACAGTTTGCA CTGGTCCAATGGCTGTCATTATACCTATAGCATCTACAGGAGTAGTATAAGGGATAAAATTTGC ACATGAGTAAGTGAGAGCAGTAAGCAGGGAATGGACAAAGCTAATAAGGAATGAGATAGATTGA TCTGGACCAAACAGAGTCACCTGAAAGAATATCTTTCCTGTAAGCACGACCTCTGAGTTCATCA AAAGAAAGAACCTTGTAAGCATGAGATGGGAATGTGGATGGTGCCTCAGTGTCTTCTCTAACGG TTGTGTGGGCAGTGAATTTTGCCATGACCATCCGATCAATAGGCCTGTACTGGGGGCGCGCCTC AGCAACTTCGAAGTACTTGATGTAATACACAGCATCCTCCTTCAGCAAAGGCTTGAATTGAGAA ACATGACGTCCTGGGATCTGCGCATCCATCATGTACCCTTGTTGATGTTTGGATGTGTAAGAGA CAAATAGCATGGAGGTAAAATAAGGCATGACCATGTGCTTATTGCTATACCTGCTCATCAAGCA GGACTAAATCAAGTCGCAGAATCTTGTCAGGTTCATTTGCTGTAGTGAACTGCGAGAAGCGCGT GACCCGCACTCGGACTTGCCAATGGTGTGATTCCTGTGTCACATTCCTTAGCATAGTGTAATCC AT

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_011447 						
					
						PMCS
						0.206997167

Conservation Information
Conserved circRNAs	osi_circ_011447
PMCS	0.206997167

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							36327931-36328533(-)
36328404-36328612(-)
						
						Potential amino acid sequence
						
							MEDGLHYAKECDTGITPLASPSAGHALLAVHYSK*(-)
MMDAQIPGRHVSQFKPLLKEDAVYYIKYFEVAEARPQYRPIDRMVMAKFTAHTTVREDTEAPST
FPSHAYKVLSFDELRGRAYRKDILSDAIGIMTAIGPVQTVSCGGVMKAVLNVHITNGRWITLC*
(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	36327931-36328533(-) 36328404-36328612(-)
Potential amino acid sequence	MEDGLHYAKECDTGITPLASPSAGHALLAVHYSK(-) MMDAQIPGRHVSQFKPLLKEDAVYYIKYFEVAEARPQYRPIDRMVMAKFTAHTTVREDTEAPST FPSHAYKVLSFDELRGRAYRKDILSDAIGIMTAIGPVQTVSCGGVMKAVLNVHITNGRWITLC (-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015;Chu et al., 2017

circRNA

Detail information of Os01g0846000_circ_g.3

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