Plant Circular RNA Database

Detail information of Os01g0321300_circ_g.8

			
						 General Information
					
						CircRNA Name
						Os01g0321300_circ_g.8
					
						ID in PlantcircBase
						osa_circ_001545
					
						Alias
						Os01circ10110
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 12242709-12244645  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer, SMALT, Segemehl
					
						Parent gene
						
							Os01g0321300						
					
						Parent gene annotation
						
							Similar to Protein translocase subunit secA. (Os01t0321300-00)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os01g0321300_circ_g.3 Os01g0321300_circ_g.4 Os01g0321300_circ_g.5 Os01g0321300_circ_g.6 Os01g0321300_circ_g.7 Os01g0321300_circ_g.9 Os01g0321300_circ_g.10 Os01g0321300_circ_g.11 Os01g0321300_circ_g.12 Os01g0321300_circ_g.13 Os01g0321300_circ_g.14 Os01g0321300_circ_g.15 Os01g0321300_circ_g.16 Os01g0321300_circ_g.17 Os01g0321300_circ_g.18 						
					
						Support reads
						2/1
					
						Tissues
						leaf and panicle/shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0321300-00:4

General Information
CircRNA Name	Os01g0321300_circ_g.8
ID in PlantcircBase	osa_circ_001545
Alias	Os01circ10110
Organism	Oryza sativa
Position	chr1: 12242709-12244645 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer, SMALT, Segemehl
Parent gene	Os01g0321300
Parent gene annotation	Similar to Protein translocase subunit secA. (Os01t0321300-00)
Parent gene strand	-
Alternative splicing	Os01g0321300_circ_g.3 Os01g0321300_circ_g.4 Os01g0321300_circ_g.5 Os01g0321300_circ_g.6 Os01g0321300_circ_g.7 Os01g0321300_circ_g.9 Os01g0321300_circ_g.10 Os01g0321300_circ_g.11 Os01g0321300_circ_g.12 Os01g0321300_circ_g.13 Os01g0321300_circ_g.14 Os01g0321300_circ_g.15 Os01g0321300_circ_g.16 Os01g0321300_circ_g.17 Os01g0321300_circ_g.18
Support reads	2/1
Tissues	leaf and panicle/shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0321300-00:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							ATTGTTCACGAGGATCGTATAGATCATTTATGTCCAGTATTTCTTCAGCGTCCGCATAGCCTTC
TTCTGTAAGTAAAACATTTCTCTGCTTTTCATCAACctcatgaggaatccctgcttcatgcaat
tgttctgataaggtttcactttgttcaacactagttgtaccaacaagcacagggcgaccaactt
tgttcatt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 619
Transcript exons: 12242709-12242876,12243000-12243113,12243709-12243826,12
244427-12244645
CTCATGAGGAATCCCTGCTTCATGCAATTGTTCTGATAAGGTTTCACTTTGTTCAACACTAGTT
GTACCAACAAGCACAGGGCGACCAACTTTGTTCATTCTTGATATCTCCACGACAGCAGCACGCC
ATTTTCCATTTGTTGCTCTAAAAACAACATCCGATTCATCCTTTCGTATCATTGGCTTGTTTGT
TGGAACAACGGTAACTTTTAGTTTGTATATGCTTTCGAACTCTTGGCTTTCCGTAGCAGCAGTT
CCAGTCATGCCACATAGTTTCGGAAATTGCCTGTAGAAAGAAGTTCTGATAACTTATTGACGCA
AGGGTAATTGTTTCATTTTGTATAGGTACACCTTCTTTAGCTTCAATTGCTTGGTGAAGACCAT
CACTCCATCTTCTGCCTGGCATTACTCGACCTGTGAACTCATCCACAATAAGCACTTCCTTGCT
ACGGACAATGTAATTCACATCTCTAAGGAAGAGTTCCTTAGCTTTGATTGCATTCAGAACATAT
GAAGCCCATTGTTCACGAGGATCGTATAGATCATTTATGTCCAGTATTTCTTCAGCGTCCGCAT
AGCCTTCTTCTGTAAGTAAAACATTTCTCTGCTTTTCATCAAC
						
					

						Genomic sequence
						
							CTCATGAGGAATCCCTGCTTCATGCAATTGTTCTGATAAGGTTTCACTTTGTTCAACACTAGTT
GTACCAACAAGCACAGGGCGACCAACTTTGTTCATTCTTGATATCTCCACGACAGCAGCACGCC
ATTTTCCATTTGTTGCTCTAAAAACAACATCCGATTCATCCTACATAGGAAGAAATGAAATCAT
CTTTTGTCAGAGAAAGGGGTTGGGGAAGTTGAATCGGAAGGACATCTCTTTCACAACTATTTAG
AATTAAAACACGCAGAGTAGAAAGAAGGCAGGCACCTTTCGTATCATTGGCTTGTTTGTTGGAA
CAACGGTAACTTTTAGTTTGTATATGCTTTCGAACTCTTGGCTTTCCGTAGCAGCAGTTCCAGT
CATGCCACATAGTTTCGGAAACTGCCACAGAATGAATCAGTTCCCTTAGAAAACAACAAACAGA
AAATATTGAAAATAAAGGGGAGATTTACCATTAAGAAACAAAATGCTAAACAATGGTTAGTACT
CCCTTCGCTCCAAAAAGACATGATGTCTAGCACTCAAAATTTGTCCCAGAAGAGTTAAGTACCC
TCAGTCCACAAATCAAGCAGTTTTATCCCTTCAATACCCATTAATGAGGGGCACTATAATCTTT
TCCCCTCACTACTACACTTTCTTGGAATGGCTAGAAATCAAGTCTTTTCGGGACAGATGGAGGA
GCTATTTCAGTTCACCTTGTATCTCAACTATATCAAAGAACTTAACTGTGGTGGTGGTTGAGTC
TGCCATCCCACCACAACCCCTAGAGTTGGCATGATCTTTTAAGGGTGGATAATTAGATCATTTG
TTCAAGTATGGCATGGCAATCTGTGGATTGTTGTTCATTTGCACATTATGTATGTTCTTCCACA
AGTATTCTGCTAGTAACAATGGGTATGATCAATGGATGCTTGCCATTTTACATATCATACAATC
ATGTGTGAGTAGCTTTCCAGCATTGGAAAAAAATTGTCACTTGCCTGTAGAAAGAAGTTCTGAT
AACTTATTGACGCAAGGGTAATTGTTTCATTTTGTATAGGTACACCTTCTTTAGCTTCAATTGC
TTGGTGAAGACCATCACTCCATCTTCTGCCCTGCCAAAAGGTAACATCTGAAAAGTGAAAACTA
AACTAAGATAGATATTGAACAAAAAGATAACCATGACTGCAGAAATAATGAAGAATGATGCAAA
CAAAAAATTTAGCATCAAATACTCACGAATTTGGAGGAAGATAACTAGGCTGGTACTTAGATCG
AATTTAAACTAAATTCTGATCCAACTCCCAAACACAACCAAGCTTTTAAAATAGAATGTTTTGC
ACAAATGGTGTGGACCCACACGCACAACCATGTAGATCAACCAGTACGTTAATGATTTAGACCA
CATATGCCACTATTAAATAGCATACTCGAACTACAACTTTAACAACATTAAAGGACAGAATATG
GAGTAAAATTATCCAATAGCTACAGATTAGTCTAATCCCTCACTGAACTTGTATTTTGAAACCA
CAAATTCCATGACAATGAGTTCATCACATAATGGAGGGAGTAATTTTCTATGTCAAATAGTTGT
TGAGAATTAGAAGTAGAGCATGCATGTACTAGTCGGGAGGCAACAGAACTAATTAATTTTTCAG
CTTTGTGGATGAGTTAATGTTTCACAATTTTGATAACAATGATGCAACACTTACTGGCATTACT
CGACCTGTGAACTCATCCACAATAAGCACTTCCTTGCTACGGACAATGTAATTCACATCTCTAA
GGAAGAGTTCCTTAGCTTTGATTGCATTCAGAACATATGAAGCCCATTGTTCACGAGGATCGTA
TAGATCATTTATGTCCAGTATTTCTTCAGCGTCCGCATAGCCTTCTTCTGTAAGTAAAACATTT
CTCTGCTTTTCATCAAC
						
					

			
		

Sequences
Splice junction sequence	ATTGTTCACGAGGATCGTATAGATCATTTATGTCCAGTATTTCTTCAGCGTCCGCATAGCCTTC TTCTGTAAGTAAAACATTTCTCTGCTTTTCATCAACctcatgaggaatccctgcttcatgcaat tgttctgataaggtttcactttgttcaacactagttgtaccaacaagcacagggcgaccaactt tgttcatt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 619 Transcript exons: 12242709-12242876,12243000-12243113,12243709-12243826,12 244427-12244645 CTCATGAGGAATCCCTGCTTCATGCAATTGTTCTGATAAGGTTTCACTTTGTTCAACACTAGTT GTACCAACAAGCACAGGGCGACCAACTTTGTTCATTCTTGATATCTCCACGACAGCAGCACGCC ATTTTCCATTTGTTGCTCTAAAAACAACATCCGATTCATCCTTTCGTATCATTGGCTTGTTTGT TGGAACAACGGTAACTTTTAGTTTGTATATGCTTTCGAACTCTTGGCTTTCCGTAGCAGCAGTT CCAGTCATGCCACATAGTTTCGGAAATTGCCTGTAGAAAGAAGTTCTGATAACTTATTGACGCA AGGGTAATTGTTTCATTTTGTATAGGTACACCTTCTTTAGCTTCAATTGCTTGGTGAAGACCAT CACTCCATCTTCTGCCTGGCATTACTCGACCTGTGAACTCATCCACAATAAGCACTTCCTTGCT ACGGACAATGTAATTCACATCTCTAAGGAAGAGTTCCTTAGCTTTGATTGCATTCAGAACATAT GAAGCCCATTGTTCACGAGGATCGTATAGATCATTTATGTCCAGTATTTCTTCAGCGTCCGCAT AGCCTTCTTCTGTAAGTAAAACATTTCTCTGCTTTTCATCAAC
Genomic sequence	CTCATGAGGAATCCCTGCTTCATGCAATTGTTCTGATAAGGTTTCACTTTGTTCAACACTAGTT GTACCAACAAGCACAGGGCGACCAACTTTGTTCATTCTTGATATCTCCACGACAGCAGCACGCC ATTTTCCATTTGTTGCTCTAAAAACAACATCCGATTCATCCTACATAGGAAGAAATGAAATCAT CTTTTGTCAGAGAAAGGGGTTGGGGAAGTTGAATCGGAAGGACATCTCTTTCACAACTATTTAG AATTAAAACACGCAGAGTAGAAAGAAGGCAGGCACCTTTCGTATCATTGGCTTGTTTGTTGGAA CAACGGTAACTTTTAGTTTGTATATGCTTTCGAACTCTTGGCTTTCCGTAGCAGCAGTTCCAGT CATGCCACATAGTTTCGGAAACTGCCACAGAATGAATCAGTTCCCTTAGAAAACAACAAACAGA AAATATTGAAAATAAAGGGGAGATTTACCATTAAGAAACAAAATGCTAAACAATGGTTAGTACT CCCTTCGCTCCAAAAAGACATGATGTCTAGCACTCAAAATTTGTCCCAGAAGAGTTAAGTACCC TCAGTCCACAAATCAAGCAGTTTTATCCCTTCAATACCCATTAATGAGGGGCACTATAATCTTT TCCCCTCACTACTACACTTTCTTGGAATGGCTAGAAATCAAGTCTTTTCGGGACAGATGGAGGA GCTATTTCAGTTCACCTTGTATCTCAACTATATCAAAGAACTTAACTGTGGTGGTGGTTGAGTC TGCCATCCCACCACAACCCCTAGAGTTGGCATGATCTTTTAAGGGTGGATAATTAGATCATTTG TTCAAGTATGGCATGGCAATCTGTGGATTGTTGTTCATTTGCACATTATGTATGTTCTTCCACA AGTATTCTGCTAGTAACAATGGGTATGATCAATGGATGCTTGCCATTTTACATATCATACAATC ATGTGTGAGTAGCTTTCCAGCATTGGAAAAAAATTGTCACTTGCCTGTAGAAAGAAGTTCTGAT AACTTATTGACGCAAGGGTAATTGTTTCATTTTGTATAGGTACACCTTCTTTAGCTTCAATTGC TTGGTGAAGACCATCACTCCATCTTCTGCCCTGCCAAAAGGTAACATCTGAAAAGTGAAAACTA AACTAAGATAGATATTGAACAAAAAGATAACCATGACTGCAGAAATAATGAAGAATGATGCAAA CAAAAAATTTAGCATCAAATACTCACGAATTTGGAGGAAGATAACTAGGCTGGTACTTAGATCG AATTTAAACTAAATTCTGATCCAACTCCCAAACACAACCAAGCTTTTAAAATAGAATGTTTTGC ACAAATGGTGTGGACCCACACGCACAACCATGTAGATCAACCAGTACGTTAATGATTTAGACCA CATATGCCACTATTAAATAGCATACTCGAACTACAACTTTAACAACATTAAAGGACAGAATATG GAGTAAAATTATCCAATAGCTACAGATTAGTCTAATCCCTCACTGAACTTGTATTTTGAAACCA CAAATTCCATGACAATGAGTTCATCACATAATGGAGGGAGTAATTTTCTATGTCAAATAGTTGT TGAGAATTAGAAGTAGAGCATGCATGTACTAGTCGGGAGGCAACAGAACTAATTAATTTTTCAG CTTTGTGGATGAGTTAATGTTTCACAATTTTGATAACAATGATGCAACACTTACTGGCATTACT CGACCTGTGAACTCATCCACAATAAGCACTTCCTTGCTACGGACAATGTAATTCACATCTCTAA GGAAGAGTTCCTTAGCTTTGATTGCATTCAGAACATATGAAGCCCATTGTTCACGAGGATCGTA TAGATCATTTATGTCCAGTATTTCTTCAGCGTCCGCATAGCCTTCTTCTGTAAGTAAAACATTT CTCTGCTTTTCATCAAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_002021* 						
					
						PMCS
						0.344033411

Conservation Information
Conserved circRNAs	osi_circ_002021*
PMCS	0.344033411

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							12243097-12242874(-)
12242733-12244575(-)
						
						Potential amino acid sequence
						
							MTGTAATESQEFESIYKLKVTVVPTNKPMIRKDESDVVFRATNGKWRAAVVEISRMNKVGRPVL
VGTTSVEQSETLSEQLHEAGIPHEVDEKQRNVLLTEEGYADAEEILDINDLYDPREQWASYVLN
AIKAKELFLRDVNYIVRSKEVLIVDEFTGRVMPGRRWSDGLHQAIEAKEGVPIQNETITLASIS
YQNFFLQAISETMWHDWNCCYGKPRVRKHIQTKSYRCSNKQANDTKG*(-)
MKQGFLMRLMKSREMFYLQKKAMRTLKKYWT*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	12243097-12242874(-) 12242733-12244575(-)
Potential amino acid sequence	MTGTAATESQEFESIYKLKVTVVPTNKPMIRKDESDVVFRATNGKWRAAVVEISRMNKVGRPVL VGTTSVEQSETLSEQLHEAGIPHEVDEKQRNVLLTEEGYADAEEILDINDLYDPREQWASYVLN AIKAKELFLRDVNYIVRSKEVLIVDEFTGRVMPGRRWSDGLHQAIEAKEGVPIQNETITLASIS YQNFFLQAISETMWHDWNCCYGKPRVRKHIQTKSYRCSNKQANDTKG(-) MKQGFLMRLMKSREMFYLQKKAMRTLKKYWT(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015;Chu et al., 2017

circRNA

Detail information of Os01g0321300_circ_g.8

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