Plant Circular RNA Database

Detail information of Cotton_A_07150_circ_g.2

			
						 General Information
					
						CircRNA Name
						Cotton_A_07150_circ_g.2
					
						ID in PlantcircBase
						gar_circ_000747
					
						Alias
						CA_chr6_BGI-A2_v1.0:74139111|74140037
					
						Organism
						
							Gossypium arboreum						
					
						Position
						
							chrCA_chr6_BGI-A2_v1.0: 74139111-74140037  JBrowse»						
					
						Reference genome
						
							BGI-A2_v1.0						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Cotton_A_07150						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							Cotton_A_07150_circ_g.1 						
					
						Support reads
						12
					
						Tissues
						ovule
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Cotton_A_07150:2

General Information
CircRNA Name	Cotton_A_07150_circ_g.2
ID in PlantcircBase	gar_circ_000747
Alias	CA_chr6_BGI-A2_v1.0:74139111\|74140037
Organism	Gossypium arboreum
Position	chrCA_chr6_BGI-A2_v1.0: 74139111-74140037 JBrowse»
Reference genome	BGI-A2_v1.0
Type	e-circRNA
Identification method	CIRI
Parent gene	Cotton_A_07150
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	Cotton_A_07150_circ_g.1
Support reads	12
Tissues	ovule
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Cotton_A_07150:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GGGAATTACAGGATATTATCCATTTGAAAGTCTTCGAGCTGCTGTGGATATCCTCTTTCTACGT
GGTGGTTCTGATTTGGTGGTTGCCAAGCAAGCGATTattatcttgagatagaagagatgaaaaa
tcggttgggtttgcttctaaaattttctgtacgtctgaccagtttatccaatgtacttgaagtt
ttggagtc

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							ATTATCTTGAGATAGAAGAGATGAAAAATCGGTTGGGTTTGCTTCTAAAATTTTCTGTACGTCT
GACCAGTTTATCCAATGTACTTGAAGTTTTGGAGTCTTCTTTCAAGGGCCGTCTTTCAGCACAG
CTTCATGACTTGCACCACCTTCAAGAGAGTATATTGAAGACAAAGCAGGTTTGATTCAGTTAAG
CGAATGGTTCATCATATTTTAGCATAATTTAAGAGCAATGTGATTTTGGCAAAGTCTCAGAACC
TGCAATCATGAAAAGCAGCTGAGTAAAGATTAATAGCAGTTTGAGTTCAGAATTTGTGAGAAAT
TAATGGCTGTCATTTGATCATTGCTCTTATTTGAACTTCTTATGACAATGTTGCTATGTCCACC
TTTTGTCTTATAGTACTGTACATTATTGTATCATTAATTTCAGACTCATTTTGACCTTTCACCG
CGCAAAATAAACAAAATATAGAATTTTAAATATGTAATGCAATAATCACCATTTTCCACAATAT
TTGTTTTATTTTTCAGCATCTTGAGATCACAATATGGTGTATAAGGCACCACTTCTTGGAACAA
GTGAGGTCTCGTCATGCTGATTTCACATCCTGGCGTAATCATGTCCGTGAAAGGAAATCAGCTG
CAGTCGTGCGGGCGTGGCCTCCGGCTGATGTGTTAGATCTCTCAACCAACTCCACTGGACAGGA
AGCTTCTCTTTTCATTGAGGATGCACTAGAAAATCTTGACATATACGAGGAGATAGGAGAAGAG
TCAGATTTTGCATTTTTGAAGAAGAATGGCGCCTTGTCCTTTCTTCAGTCTAAAATAGGGGGAA
TTACAGGATATTATCCATTTGAAAGTCTTCGAGCTGCTGTGGATATCCTCTTTCTACGTGGTGG
TTCTGATTTGGTGGTTGCCAAGCAAGCGATT

Sequences
Splice junction sequence	GGGAATTACAGGATATTATCCATTTGAAAGTCTTCGAGCTGCTGTGGATATCCTCTTTCTACGT GGTGGTTCTGATTTGGTGGTTGCCAAGCAAGCGATTattatcttgagatagaagagatgaaaaa tcggttgggtttgcttctaaaattttctgtacgtctgaccagtttatccaatgtacttgaagtt ttggagtc
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	ATTATCTTGAGATAGAAGAGATGAAAAATCGGTTGGGTTTGCTTCTAAAATTTTCTGTACGTCT GACCAGTTTATCCAATGTACTTGAAGTTTTGGAGTCTTCTTTCAAGGGCCGTCTTTCAGCACAG CTTCATGACTTGCACCACCTTCAAGAGAGTATATTGAAGACAAAGCAGGTTTGATTCAGTTAAG CGAATGGTTCATCATATTTTAGCATAATTTAAGAGCAATGTGATTTTGGCAAAGTCTCAGAACC TGCAATCATGAAAAGCAGCTGAGTAAAGATTAATAGCAGTTTGAGTTCAGAATTTGTGAGAAAT TAATGGCTGTCATTTGATCATTGCTCTTATTTGAACTTCTTATGACAATGTTGCTATGTCCACC TTTTGTCTTATAGTACTGTACATTATTGTATCATTAATTTCAGACTCATTTTGACCTTTCACCG CGCAAAATAAACAAAATATAGAATTTTAAATATGTAATGCAATAATCACCATTTTCCACAATAT TTGTTTTATTTTTCAGCATCTTGAGATCACAATATGGTGTATAAGGCACCACTTCTTGGAACAA GTGAGGTCTCGTCATGCTGATTTCACATCCTGGCGTAATCATGTCCGTGAAAGGAAATCAGCTG CAGTCGTGCGGGCGTGGCCTCCGGCTGATGTGTTAGATCTCTCAACCAACTCCACTGGACAGGA AGCTTCTCTTTTCATTGAGGATGCACTAGAAAATCTTGACATATACGAGGAGATAGGAGAAGAG TCAGATTTTGCATTTTTGAAGAAGAATGGCGCCTTGTCCTTTCTTCAGTCTAAAATAGGGGGAA TTACAGGATATTATCCATTTGAAAGTCTTCGAGCTGCTGTGGATATCCTCTTTCTACGTGGTGG TTCTGATTTGGTGGTTGCCAAGCAAGCGATT

			
						 Conservation Information
					
						Conserved circRNAs
						
							gra_circ_000874* 						
					
						PMCS

Conservation Information
Conserved circRNAs	gra_circ_000874*
PMCS

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							74139131-74139122(+)
						
						Potential amino acid sequence
						
							MKNRLGLLLKFSVRLTSLSNVLEVLESSFKGRLSAQLHDLHHLQESILKTKQHLEITIWCIRHH
FLEQVRSRHADFTSWRNHVRERKSAAVVRAWPPADVLDLSTNSTGQEASLFIEDALENLDIYEE
IGEESDFAFLKKNGALSFLQSKIGGITGYYPFESLRAAVDILFLRGGSDLVVAKQAIIILR*(+
)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	74139131-74139122(+)
Potential amino acid sequence	MKNRLGLLLKFSVRLTSLSNVLEVLESSFKGRLSAQLHDLHHLQESILKTKQHLEITIWCIRHH FLEQVRSRHADFTSWRNHVRERKSAAVVRAWPPADVLDLSTNSTGQEASLFIEDALENLDIYEE IGEESDFAFLKKNGALSFLQSKIGGITGYYPFESLRAAVDILFLRGGSDLVVAKQAIIILR*(+ )
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of Cotton_A_07150_circ_g.2

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