Plant Circular RNA Database

Detail information of AT5G53860_circ_g.2

			
						 General Information
					
						CircRNA Name
						AT5G53860_circ_g.2
					
						ID in PlantcircBase
						ath_circ_043993
					
						Alias
						At_ciR2376
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 21865932-21866565  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ, CIRI-full
					
						Parent gene
						
							AT5G53860						
					
						Parent gene annotation
						
							Embryo defective 2737
							
						Parent gene strand
						+
					
						Alternative splicing
						
							ENSRNA049493778_circ_g.1 ENSRNA049493778_circ_g.2 AT5G53740_circ_g.1 5_circ_ag.2 5_circ_ag.3 AT5G53740_circ_g.4 AT5G53740_circ_g.5 5_circ_ag.6 AT5G53760_circ_g.1 AT5G53770_circ_g.1 AT5G53770_circ_g.2 5_circ_ig.3 AT5G53850_circ_g.1 AT5G53850_circ_g.2 AT5G53850_circ_g.3 AT5G53850_circ_g.4 AT5G53850_circ_g.5 AT5G53860_circ_g.1 AT5G53860_circ_g.3 AT5G53860_circ_g.4 AT5G53860_circ_g.5 AT5G53860_circ_g.6 AT5G53860_circ_g.7 AT5G53860_circ_g.8 AT5G53860_circ_g.9 AT5G53860_circ_g.10 AT5G53860_circ_g.11 AT5G53880_circ_g.1 AT5G53905_circ_g.1 5_circ_ag.2 AT5G53905_circ_g.3 5_circ_ag.4 AT5G53920_circ_g.1 AT5G53940_circ_g.1 AT5G53940_circ_g.2 AT5G53970_circ_g.1 AT5G53970_circ_g.2 AT5G54000_circ_g.1 AT5G54060_circ_g.1 ENSRNA049493776_circ_g.1 ENSRNA049493776_circ_g.2 ENSRNA049493776_circ_g.3 5_circ_ag.4 5_circ_ag.5 5_circ_ag.6 AT5G54080_circ_g.1 AT5G54080_circ_g.2 AT5G54080_circ_g.3 AT5G54080_circ_g.4 AT5G54090_circ_g.1 AT5G54090_circ_g.2 AT5G54090_circ_g.3 AT5G54090_circ_g.4 AT5G54100_circ_g.1 AT5G54130_circ_g.1 AT5G54130_circ_g.2 AT5G54130_circ_g.3 AT5G54130_circ_g.4 AT5G54130_circ_g.5 AT5G54160_circ_g.1 AT5G54160_circ_g.2 AT5G54170_circ_g.1 ENSRNA049493783_circ_g.1 ENSRNA049493783_circ_g.2 ENSRNA049493783_circ_g.3 ENSRNA049493783_circ_g.4 						
					
						Support reads
						3
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G53860.5:3
AT5G53860.4:3
AT5G53860.3:3
AT5G53860.1:3
AT5G53860.2:3

General Information
CircRNA Name	AT5G53860_circ_g.2
ID in PlantcircBase	ath_circ_043993
Alias	At_ciR2376
Organism	Arabidpsis thaliana
Position	chr5: 21865932-21866565 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	find_circ, CIRI-full
Parent gene	AT5G53860
Parent gene annotation	Embryo defective 2737
Parent gene strand	+
Alternative splicing	ENSRNA049493778_circ_g.1 ENSRNA049493778_circ_g.2 AT5G53740_circ_g.1 5_circ_ag.2 5_circ_ag.3 AT5G53740_circ_g.4 AT5G53740_circ_g.5 5_circ_ag.6 AT5G53760_circ_g.1 AT5G53770_circ_g.1 AT5G53770_circ_g.2 5_circ_ig.3 AT5G53850_circ_g.1 AT5G53850_circ_g.2 AT5G53850_circ_g.3 AT5G53850_circ_g.4 AT5G53850_circ_g.5 AT5G53860_circ_g.1 AT5G53860_circ_g.3 AT5G53860_circ_g.4 AT5G53860_circ_g.5 AT5G53860_circ_g.6 AT5G53860_circ_g.7 AT5G53860_circ_g.8 AT5G53860_circ_g.9 AT5G53860_circ_g.10 AT5G53860_circ_g.11 AT5G53880_circ_g.1 AT5G53905_circ_g.1 5_circ_ag.2 AT5G53905_circ_g.3 5_circ_ag.4 AT5G53920_circ_g.1 AT5G53940_circ_g.1 AT5G53940_circ_g.2 AT5G53970_circ_g.1 AT5G53970_circ_g.2 AT5G54000_circ_g.1 AT5G54060_circ_g.1 ENSRNA049493776_circ_g.1 ENSRNA049493776_circ_g.2 ENSRNA049493776_circ_g.3 5_circ_ag.4 5_circ_ag.5 5_circ_ag.6 AT5G54080_circ_g.1 AT5G54080_circ_g.2 AT5G54080_circ_g.3 AT5G54080_circ_g.4 AT5G54090_circ_g.1 AT5G54090_circ_g.2 AT5G54090_circ_g.3 AT5G54090_circ_g.4 AT5G54100_circ_g.1 AT5G54130_circ_g.1 AT5G54130_circ_g.2 AT5G54130_circ_g.3 AT5G54130_circ_g.4 AT5G54130_circ_g.5 AT5G54160_circ_g.1 AT5G54160_circ_g.2 AT5G54170_circ_g.1 ENSRNA049493783_circ_g.1 ENSRNA049493783_circ_g.2 ENSRNA049493783_circ_g.3 ENSRNA049493783_circ_g.4
Support reads	3
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G53860.5:3 AT5G53860.4:3 AT5G53860.3:3 AT5G53860.1:3 AT5G53860.2:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TGCTATAGTTGAGAATCGAGCTGAGTTAGTTCATCTTCCCTCCTCCTTCAACCATAGTGCTCCA
TTGCCATCAAAAGACTGCCCAACTTGTGATGGAACGgcggttgctacgcttggaacttatgaca
ttgcattggatttgggaaagaaagtcatatgccaaaggtcagcttcaagatttgatgctttatt
ctgggtca

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 297
Transcript exons: 21865932-21865996,21866133-21866231,21866433-21866565
GCGGTTGCTACGCTTGGAACTTATGACATTGCATTGGATTTGGGAAAGAAAGTCATATGCCAAA
GGGATTGCAAAACTTGTAATGGGTGGCAGGCATTACGGTGCACTATGTGCAAAGGAACAGGGAG
TGTTCATTACCAAATCAAAGATTACAACTTGAGAAGTGGAGAGAAACCAACAGCAGATTGTGTC
GCAGATGCTATAGTTGAGAATCGAGCTGAGTTAGTTCATCTTCCCTCCTCCTTCAACCATAGTG
CTCCATTGCCATCAAAAGACTGCCCAACTTGTGATGGAACG
						
						Genomic sequence
						
							GCGGTTGCTACGCTTGGAACTTATGACATTGCATTGGATTTGGGAAAGAAAGTCATATGCCAAA
GGTCAGCTTCAAGATTTGATGCTTTATTCTGGGTCAGAGAATTTTTGAGAAACCTCAGTTCTCA
CACTTCCTCGCTATGTTTTTGGTTCTACTGTTTGAGTTCATCCGATGAGATGAAAACATTTTTT
GTTGGTCAGGGATTGCAAAACTTGTAATGGGTGGCAGGCATTACGGTGCACTATGTGCAAAGGA
ACAGGGAGTGTTCATTACCAAATCAAAGATTACAACTTGAGAAGGTGGGATTATCTCTTGTTTA
ACCCAAGTTATATCGCTTAAACTACTAGGCTTCATGAATAGATCCACTTTATTCTGACGGTCTC
TTTGTGTTCTCTGTATATTAATGTCGACAGTTCCATTTGGCAATGCTTAAGCTATCCTAGTGAT
ATTCTAACAGATAAAAGGATTTAAAGATTTATCCTTTCTGTCTTTACTTGTAGTGGAGAGAAAC
CAACAGCAGATTGTGTCGCAGATGCTATAGTTGAGAATCGAGCTGAGTTAGTTCATCTTCCCTC
CTCCTTCAACCATAGTGCTCCATTGCCATCAAAAGACTGCCCAACTTGTGATGGAACG

Sequences
Splice junction sequence	TGCTATAGTTGAGAATCGAGCTGAGTTAGTTCATCTTCCCTCCTCCTTCAACCATAGTGCTCCA TTGCCATCAAAAGACTGCCCAACTTGTGATGGAACGgcggttgctacgcttggaacttatgaca ttgcattggatttgggaaagaaagtcatatgccaaaggtcagcttcaagatttgatgctttatt ctgggtca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 297 Transcript exons: 21865932-21865996,21866133-21866231,21866433-21866565 GCGGTTGCTACGCTTGGAACTTATGACATTGCATTGGATTTGGGAAAGAAAGTCATATGCCAAA GGGATTGCAAAACTTGTAATGGGTGGCAGGCATTACGGTGCACTATGTGCAAAGGAACAGGGAG TGTTCATTACCAAATCAAAGATTACAACTTGAGAAGTGGAGAGAAACCAACAGCAGATTGTGTC GCAGATGCTATAGTTGAGAATCGAGCTGAGTTAGTTCATCTTCCCTCCTCCTTCAACCATAGTG CTCCATTGCCATCAAAAGACTGCCCAACTTGTGATGGAACG
Genomic sequence	GCGGTTGCTACGCTTGGAACTTATGACATTGCATTGGATTTGGGAAAGAAAGTCATATGCCAAA GGTCAGCTTCAAGATTTGATGCTTTATTCTGGGTCAGAGAATTTTTGAGAAACCTCAGTTCTCA CACTTCCTCGCTATGTTTTTGGTTCTACTGTTTGAGTTCATCCGATGAGATGAAAACATTTTTT GTTGGTCAGGGATTGCAAAACTTGTAATGGGTGGCAGGCATTACGGTGCACTATGTGCAAAGGA ACAGGGAGTGTTCATTACCAAATCAAAGATTACAACTTGAGAAGGTGGGATTATCTCTTGTTTA ACCCAAGTTATATCGCTTAAACTACTAGGCTTCATGAATAGATCCACTTTATTCTGACGGTCTC TTTGTGTTCTCTGTATATTAATGTCGACAGTTCCATTTGGCAATGCTTAAGCTATCCTAGTGAT ATTCTAACAGATAAAAGGATTTAAAGATTTATCCTTTCTGTCTTTACTTGTAGTGGAGAGAAAC CAACAGCAGATTGTGTCGCAGATGCTATAGTTGAGAATCGAGCTGAGTTAGTTCATCTTCCCTC CTCCTTCAACCATAGTGCTCCATTGCCATCAAAAGACTGCCCAACTTGTGATGGAACG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.203850467

Conservation Information
Conserved circRNAs	NA
PMCS	0.203850467

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							21866176-21866562(+)
						
						Potential amino acid sequence
						
							MCKGTGSVHYQIKDYNLRSGEKPTADCVADAIVENRAELVHLPSSFNHSAPLPSKDCPTCDGTA
VATLGTYDIALDLGKKVICQRDCKTCNGWQALRCTMCKGTGSVHYQIKDYNLRSGEKPTADCVA
DAIVENRAELVHLPSSFNHSAPLPSKDCPTCDGTAVATLGTYDIALDLGKKVICQRDCKTCNGW
QALRCTMCKGTGSVHYQIKDYNLRSGEKPTADCVADAIVENRAELVHLPSSFNHSAPLPSKDCP
TCDGTAVATLGTYDIALDLGKKVICQRDCKTCNGWQALRCTMCKGTGSVHYQIKDYNLRSGEKP
TADCVADAIVENRAELVHLPSSFNHSAPLPSKDCPTCDGT(+)
						
						Sponge-miRNAs
						
							ath-miR1886.2 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	21866176-21866562(+)
Potential amino acid sequence	MCKGTGSVHYQIKDYNLRSGEKPTADCVADAIVENRAELVHLPSSFNHSAPLPSKDCPTCDGTA VATLGTYDIALDLGKKVICQRDCKTCNGWQALRCTMCKGTGSVHYQIKDYNLRSGEKPTADCVA DAIVENRAELVHLPSSFNHSAPLPSKDCPTCDGTAVATLGTYDIALDLGKKVICQRDCKTCNGW QALRCTMCKGTGSVHYQIKDYNLRSGEKPTADCVADAIVENRAELVHLPSSFNHSAPLPSKDCP TCDGTAVATLGTYDIALDLGKKVICQRDCKTCNGWQALRCTMCKGTGSVHYQIKDYNLRSGEKP TADCVADAIVENRAELVHLPSSFNHSAPLPSKDCPTCDGT(+)
Sponge-miRNAs	ath-miR1886.2
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of AT5G53860_circ_g.2

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