Plant Circular RNA Database

Detail information of Os02g0813800_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os02g0813800_circ_g.2
					
						ID in PlantcircBase
						osa_circ_017124
					
						Alias
						Os_ciR8201
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 34868511-34870379  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder, find_circ
					
						Parent gene
						
							Os02g0813800						
					
						Parent gene annotation
						
							Similar to STYLOSA protein. (Os02t0813800-01);Non-protein coding
 transcript. (Os02t0813800-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os02g0813800_circ_g.1 Os02g0813800_circ_g.3 Os02g0813800_circ_g.4 						
					
						Support reads
						2/5
					
						Tissues
						root/shoot, root, seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0813800-01:7

General Information
CircRNA Name	Os02g0813800_circ_g.2
ID in PlantcircBase	osa_circ_017124
Alias	Os_ciR8201
Organism	Oryza sativa
Position	chr2: 34868511-34870379 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, PcircRNA_finder, find_circ
Parent gene	Os02g0813800
Parent gene annotation	Similar to STYLOSA protein. (Os02t0813800-01);Non-protein coding transcript. (Os02t0813800-02)
Parent gene strand	-
Alternative splicing	Os02g0813800_circ_g.1 Os02g0813800_circ_g.3 Os02g0813800_circ_g.4
Support reads	2/5
Tissues	root/shoot, root, seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0813800-01:7

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GCCGCTGCAACCTCAGAATGCTTCTCATTTGTTCTTGCAATAAATATATCCCAAAAAACAGACC
ACCACTCAAAGAGAAACCCTCCAGGAGCATCAATTGctgttgcatttgctgttgctggctttgc
tgctgctgttgttgctggctctgctgctgctgctgttcctgcggttgctgggaagatgtctgtt
gtacctga

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 1043
Transcript exons: 34868511-34868606,34868694-34868996,34869082-34869131,34
869510-34869621,34869711-34869786,34869896-34870191,3487
0270-34870379
CTGTTGCATTTGCTGTTGCTGGCTTTGCTGCTGCTGTTGTTGCTGGCTCTGCTGCTGCTGCTGT
TCCTGCGGTTGCTGGGAAGATGTCTGTTGTACCTTTATGAGATACTCTTGGTCTGACCTAACTT
TGGGTGAACTGGATTGCATTGGGGAACTAATACACCCATCAGTTCCAGCAGGCTGTCCATCCTT
ACCATTCAAGCCACCCCTGGTTAGTGCTGTATATCTCCGGGGATCCAAATCCCCATAGTTACTT
GAGTTGCTAAGATTGCCTTGTGCTTGGGCCTGTGCTAAGAATTGTTGCTGCTGCTGTGGTGACA
TAAGTTGAAACTGTGACTGTGTTGAAAGAAATGGCTTCTGCATTTGAGCACCTAAATTTGGTCG
CAATTGGTCAATTCCAGTTAATGGCCACCCTTTCAATGGTAGACCGCTTACTCCTTGGTTTAGT
CCTGCACCGCCCAATCCAGGTTTTGGCTGAATAATACCCTGTCCATATAAGGATGATGGGTCCA
TGGGCAAAGATCTTTGGGCAACACCCATATTACCTTCACTTTTTATATCCATAGTTTGTTGATT
CCTAGACTGGATTTGTTGCAGCGTTGTAGACACATTTCCGGGAGTTCCGGGAACTAATTGCCCT
GCATGATTTGTTGCAGCTGACTTGAGAAGAGCCATCCTACTAGCATCAAGAAGCTGTGATCCCT
CAGAGTCCAAGGATTGAGGGTGCTTCAAGCGCTCTTCATACATCTTGGCAGCCAAAACACTAGC
TGTTGAATGCCCCAGAATGCCATCAGAATTTAGGGTGTTTATTGGACCATTAAGTGAAGGATGA
CTTGCATTAGTTCGCTGCAGTTGAGCATGTCTGTGTTGTATTAGTTGCAACTGCTGCATCTGCA
TCTGCTGTTGGTGCTCTCTCGCTTTGATTTGCTGTGCCTCTAGGTATGCCGCTGCAACCTCAGA
ATGCTTCTCATTTGTTCTTGCAATAAATATATCCCAAAAAACAGACCACCACTCAAAGAGAAAC
CCTCCAGGAGCATCAATTG
						
					

						Genomic sequence
						
							CTGTTGCATTTGCTGTTGCTGGCTTTGCTGCTGCTGTTGTTGCTGGCTCTGCTGCTGCTGCTGT
TCCTGCGGTTGCTGGGAAGATGTCTGTTGTACCTGAATAACTTGCAGGTACAGTCAGATGGTTG
AACACTAGAGACAATGAAAACACAAACAGAAGTGCGCTTCCAAAAATAAACCAACCTTTATGAG
ATACTCTTGGTCTGACCTAACTTTGGGTGAACTGGATTGCATTGGGGAACTAATACACCCATCA
GTTCCAGCAGGCTGTCCATCCTTACCATTCAAGCCACCCCTGGTTAGTGCTGTATATCTCCGGG
GATCCAAATCCCCATAGTTACTTGAGTTGCTAAGATTGCCTTGTGCTTGGGCCTGTGCTAAGAA
TTGTTGCTGCTGCTGTGGTGACATAAGTTGAAACTGTGACTGTGTTGAAAGAAATGGCTTCTGC
ATTTGAGCACCTAAATTTGGTCGCAATTGGTCAATTCCCTGTAGGATGGCAGCACTGGCATTAA
TTGGTGATTAGATAGTAATAAAAACATGAAGAAAGGGGACAATAGATAAATGAACTTACAGTTA
ATGGCCACCCTTTCAATGGTAGACCGCTTACTCCTTGGTTTAGTCCTGTTAACAGCAGAAGAAG
AAATTTCAACAATTGACAAACAAAAAGGACATCCAAAAAATCAGAACAAACAAAACGAGAACAG
GATGGTTTTCTACATTGAGAACAACAACATTTGCTTTTTAGACTAGAAATATATCCATGCAATA
TTCACCACAAAAGCCAACCAGAAACAGCATAATAATTAATTCAGTACTTGTTTAATATCCATTT
GTTTTGGCAATTTATATTTACTATTCGCAACCTTAGATGTTACTGTTATTACAAGAATGACAGA
CTAGTAAATCGATTGTTGTTAGAGAAGGCAAGTAACTCCACATTATTGCAGTTTCACTTTAGCA
ACAAAAGTGACCTCACAATATACATAGGAAAGGACTAACCTGCACCGCCCAATCCAGGTTTTGG
CTGAATAATACCCTGTCCATATAAGGATGATGGGTCCATGGGCAAAGATCTTTGGGCAACACCC
ATATTACCTTCACTTTTTATATCCTGTTGGAAAGAACATCTATCTTTTTACTTTTACATTTTAA
TGGAGCAAACAAAATGACACAAGATGAGCACAAGAAGCAACAACTCACCATAGTTTGTTGATTC
CTAGACTGGATTTGTTGCAGCGTTGTAGACACATTTCCGGGAGTTCCGGGAACTAATTGCCTAT
GAGATTAAGATATATGATTAGTGATCATGCCAGTAACATATCAAAATTCAATTTCTCCCATCTC
TCTCTTATATAAAAGCTTATCAAAAACTAAAAGAGAATCACCCTGCATGATTTGTTGCAGCTGA
CTTGAGAAGAGCCATCCTACTAGCATCAAGAAGCTGTGATCCCTCAGAGTCCAAGGATTGAGGG
TGCTTCAAGCGCTCTTCATACATCTTGGCAGCCAAAACACTAGCTGTTGAATGCCCCAGAATGC
CATCAGAATTTAGGGTGTTTATTGGACCATTAAGTGAAGGATGACTTGCATTAGTTCGCTGCAG
TTGAGCATGTCTGTGTTGTATTAGTTGCAACTGCTGCATCTGCATCTGCTGTTGGTGCTCTCTC
GCTTTGATTTGCTGTGCCTATGTTTCCGACAGAAATACACGTGGATCAGATCAAGAGTAATAGA
TGCATGGCGACTTAAAAAATTCCCAGGATACCTCTAGGTATGCCGCTGCAACCTCAGAATGCTT
CTCATTTGTTCTTGCAATAAATATATCCCAAAAAACAGACCACCACTCAAAGAGAAACCCTCCA
GGAGCATCAATTG
						
					

			
		

Sequences
Splice junction sequence	GCCGCTGCAACCTCAGAATGCTTCTCATTTGTTCTTGCAATAAATATATCCCAAAAAACAGACC ACCACTCAAAGAGAAACCCTCCAGGAGCATCAATTGctgttgcatttgctgttgctggctttgc tgctgctgttgttgctggctctgctgctgctgctgttcctgcggttgctgggaagatgtctgtt gtacctga
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 1043 Transcript exons: 34868511-34868606,34868694-34868996,34869082-34869131,34 869510-34869621,34869711-34869786,34869896-34870191,3487 0270-34870379 CTGTTGCATTTGCTGTTGCTGGCTTTGCTGCTGCTGTTGTTGCTGGCTCTGCTGCTGCTGCTGT TCCTGCGGTTGCTGGGAAGATGTCTGTTGTACCTTTATGAGATACTCTTGGTCTGACCTAACTT TGGGTGAACTGGATTGCATTGGGGAACTAATACACCCATCAGTTCCAGCAGGCTGTCCATCCTT ACCATTCAAGCCACCCCTGGTTAGTGCTGTATATCTCCGGGGATCCAAATCCCCATAGTTACTT GAGTTGCTAAGATTGCCTTGTGCTTGGGCCTGTGCTAAGAATTGTTGCTGCTGCTGTGGTGACA TAAGTTGAAACTGTGACTGTGTTGAAAGAAATGGCTTCTGCATTTGAGCACCTAAATTTGGTCG CAATTGGTCAATTCCAGTTAATGGCCACCCTTTCAATGGTAGACCGCTTACTCCTTGGTTTAGT CCTGCACCGCCCAATCCAGGTTTTGGCTGAATAATACCCTGTCCATATAAGGATGATGGGTCCA TGGGCAAAGATCTTTGGGCAACACCCATATTACCTTCACTTTTTATATCCATAGTTTGTTGATT CCTAGACTGGATTTGTTGCAGCGTTGTAGACACATTTCCGGGAGTTCCGGGAACTAATTGCCCT GCATGATTTGTTGCAGCTGACTTGAGAAGAGCCATCCTACTAGCATCAAGAAGCTGTGATCCCT CAGAGTCCAAGGATTGAGGGTGCTTCAAGCGCTCTTCATACATCTTGGCAGCCAAAACACTAGC TGTTGAATGCCCCAGAATGCCATCAGAATTTAGGGTGTTTATTGGACCATTAAGTGAAGGATGA CTTGCATTAGTTCGCTGCAGTTGAGCATGTCTGTGTTGTATTAGTTGCAACTGCTGCATCTGCA TCTGCTGTTGGTGCTCTCTCGCTTTGATTTGCTGTGCCTCTAGGTATGCCGCTGCAACCTCAGA ATGCTTCTCATTTGTTCTTGCAATAAATATATCCCAAAAAACAGACCACCACTCAAAGAGAAAC CCTCCAGGAGCATCAATTG
Genomic sequence	CTGTTGCATTTGCTGTTGCTGGCTTTGCTGCTGCTGTTGTTGCTGGCTCTGCTGCTGCTGCTGT TCCTGCGGTTGCTGGGAAGATGTCTGTTGTACCTGAATAACTTGCAGGTACAGTCAGATGGTTG AACACTAGAGACAATGAAAACACAAACAGAAGTGCGCTTCCAAAAATAAACCAACCTTTATGAG ATACTCTTGGTCTGACCTAACTTTGGGTGAACTGGATTGCATTGGGGAACTAATACACCCATCA GTTCCAGCAGGCTGTCCATCCTTACCATTCAAGCCACCCCTGGTTAGTGCTGTATATCTCCGGG GATCCAAATCCCCATAGTTACTTGAGTTGCTAAGATTGCCTTGTGCTTGGGCCTGTGCTAAGAA TTGTTGCTGCTGCTGTGGTGACATAAGTTGAAACTGTGACTGTGTTGAAAGAAATGGCTTCTGC ATTTGAGCACCTAAATTTGGTCGCAATTGGTCAATTCCCTGTAGGATGGCAGCACTGGCATTAA TTGGTGATTAGATAGTAATAAAAACATGAAGAAAGGGGACAATAGATAAATGAACTTACAGTTA ATGGCCACCCTTTCAATGGTAGACCGCTTACTCCTTGGTTTAGTCCTGTTAACAGCAGAAGAAG AAATTTCAACAATTGACAAACAAAAAGGACATCCAAAAAATCAGAACAAACAAAACGAGAACAG GATGGTTTTCTACATTGAGAACAACAACATTTGCTTTTTAGACTAGAAATATATCCATGCAATA TTCACCACAAAAGCCAACCAGAAACAGCATAATAATTAATTCAGTACTTGTTTAATATCCATTT GTTTTGGCAATTTATATTTACTATTCGCAACCTTAGATGTTACTGTTATTACAAGAATGACAGA CTAGTAAATCGATTGTTGTTAGAGAAGGCAAGTAACTCCACATTATTGCAGTTTCACTTTAGCA ACAAAAGTGACCTCACAATATACATAGGAAAGGACTAACCTGCACCGCCCAATCCAGGTTTTGG CTGAATAATACCCTGTCCATATAAGGATGATGGGTCCATGGGCAAAGATCTTTGGGCAACACCC ATATTACCTTCACTTTTTATATCCTGTTGGAAAGAACATCTATCTTTTTACTTTTACATTTTAA TGGAGCAAACAAAATGACACAAGATGAGCACAAGAAGCAACAACTCACCATAGTTTGTTGATTC CTAGACTGGATTTGTTGCAGCGTTGTAGACACATTTCCGGGAGTTCCGGGAACTAATTGCCTAT GAGATTAAGATATATGATTAGTGATCATGCCAGTAACATATCAAAATTCAATTTCTCCCATCTC TCTCTTATATAAAAGCTTATCAAAAACTAAAAGAGAATCACCCTGCATGATTTGTTGCAGCTGA CTTGAGAAGAGCCATCCTACTAGCATCAAGAAGCTGTGATCCCTCAGAGTCCAAGGATTGAGGG TGCTTCAAGCGCTCTTCATACATCTTGGCAGCCAAAACACTAGCTGTTGAATGCCCCAGAATGC CATCAGAATTTAGGGTGTTTATTGGACCATTAAGTGAAGGATGACTTGCATTAGTTCGCTGCAG TTGAGCATGTCTGTGTTGTATTAGTTGCAACTGCTGCATCTGCATCTGCTGTTGGTGCTCTCTC GCTTTGATTTGCTGTGCCTATGTTTCCGACAGAAATACACGTGGATCAGATCAAGAGTAATAGA TGCATGGCGACTTAAAAAATTCCCAGGATACCTCTAGGTATGCCGCTGCAACCTCAGAATGCTT CTCATTTGTTCTTGCAATAAATATATCCCAAAAAACAGACCACCACTCAAAGAGAAACCCTCCA GGAGCATCAATTG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_012460 						
					
						PMCS
						0.377006572

Conservation Information
Conserved circRNAs	osi_circ_012460
PMCS	0.377006572

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							34870298-34868844(+)
34870157-34870274(-)
						
						Potential amino acid sequence
						
							MLLICSCNKYIPKNRPPLKEKPSRSINCCCICCCWLCCCCCCWLCCCCCSCGCWEDVCCTFMRY
SWSDLTLGELDCIGELIHPSVPAGCPSLPFKPPLVSAVYLRGSKSP*(+)
MQMQQLQLIQHRHAQLQRTNASHPSLNGPINTLNSDGILGHSTASVLAAKMYEERLKHPQSLDS
EGSQLLDASRMALLKSAATNHAGQLVPGTPGNVSTTLQQIQSRNQQTMDIKSEGNMGVAQRSLP
MDPSSLYGQGIIQPKPGLGGAGLNQGVSGLPLKGWPLTGIDQLRPNLGAQMQKPFLSTQSQFQL
MSPQQQQQFLAQAQAQGNLSNSSNYGDLDPRRYTALTRGGLNGKDGQPAGTDGCISSPMQSSSP
KVRSDQEYLIKVQQTSSQQPQEQQQQQSQQQQQQQSQQQQMQQQLMLLEGFSLSGGLFFGIYLL
QEQMRSILRLQRHT*(-)
						
						Sponge-miRNAs
						
							osa-miR530-3p 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	34870298-34868844(+) 34870157-34870274(-)
Potential amino acid sequence	MLLICSCNKYIPKNRPPLKEKPSRSINCCCICCCWLCCCCCCWLCCCCCSCGCWEDVCCTFMRY SWSDLTLGELDCIGELIHPSVPAGCPSLPFKPPLVSAVYLRGSKSP(+) MQMQQLQLIQHRHAQLQRTNASHPSLNGPINTLNSDGILGHSTASVLAAKMYEERLKHPQSLDS EGSQLLDASRMALLKSAATNHAGQLVPGTPGNVSTTLQQIQSRNQQTMDIKSEGNMGVAQRSLP MDPSSLYGQGIIQPKPGLGGAGLNQGVSGLPLKGWPLTGIDQLRPNLGAQMQKPFLSTQSQFQL MSPQQQQQFLAQAQAQGNLSNSSNYGDLDPRRYTALTRGGLNGKDGQPAGTDGCISSPMQSSSP KVRSDQEYLIKVQQTSSQQPQEQQQQQSQQQQQQQSQQQQMQQQLMLLEGFSLSGGLFFGIYLL QEQMRSILRLQRHT(-)
Sponge-miRNAs	osa-miR530-3p
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os02g0813800_circ_g.2

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