Plant Circular RNA Database

Detail information of Os08g0328300_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os08g0328300_circ_g.1
					
						ID in PlantcircBase
						osa_circ_036749
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr8: 14477246-14478472  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os08g0328300						
					
						Parent gene annotation
						
							ENT domain containing protein. (Os08t0328300-01);ENT domain cont
aining protein. (Os08t0328300-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os08g0328300_circ_g.2 Os08g0328300_circ_g.3 Os08g0328300_circ_g.4 						
					
						Support reads
						2
					
						Tissues
						shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os08t0328300-01:5
Os08t0328300-02:5

General Information
CircRNA Name	Os08g0328300_circ_g.1
ID in PlantcircBase	osa_circ_036749
Alias	NA
Organism	Oryza sativa
Position	chr8: 14477246-14478472 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os08g0328300
Parent gene annotation	ENT domain containing protein. (Os08t0328300-01);ENT domain cont aining protein. (Os08t0328300-02)
Parent gene strand	+
Alternative splicing	Os08g0328300_circ_g.2 Os08g0328300_circ_g.3 Os08g0328300_circ_g.4
Support reads	2
Tissues	shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os08t0328300-01:5 Os08t0328300-02:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TACTTCTGATGTCTGTTCTCATTAATCATAGGATCGTTATGCACTGGTTTATGACATAAATACA
GCAGATGAAACCTGGGAGTGGGTTGACTTTAAAGAGgaactgatgatgatcttccaccgacata
tcagaacagaggtgtaagaggcagtggacgtgttagtggtaatgggagagatattgttagcgct
gttccata

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 162
Transcript exons: 14477246-14477374,14478440-14478472
GAACTGATGATGATCTTCCACCGACATATCAGAACAGAGGTGTAAGAGGCAGTGGACGTGTTAG
TGGTAATGGGAGAGATATTGTTAGCGCTGTTCCATATAATAGAACTAAGCCACAGACAGATATG
GGATGAAACCTGGGAGTGGGTTGACTTTAAAGAG
Transcript length: 854
Transcript exons: 14477246-14477457,14477566-14477675,14477755-14478007,14
478120-14478329,14478404-14478472
GAACTGATGATGATCTTCCACCGACATATCAGAACAGAGGTGTAAGAGGCAGTGGACGTGTTAG
TGGTAATGGGAGAGATATTGTTAGCGCTGTTCCATATAATAGAACTAAGCCACAGACAGATATG
GAAACACAAATTCATCAGCTTGAGCAAGATGCATATTGTTCTGTTCTTCGTGCATTTAAAGCAC
AATCTGATGCTATTTCATGGGAAAAGGAAGGTCTTATCACTGAACTTAGAAAGGAACTTAGAGT
GTCTGACAAAGAGCACAGGGAGTTGTTAAACAGAGTGAACGGTGATGATATCATTCAAAGAATA
AGGGAATGGAGAGAGACAAAAGGTGGGCTTCAGGCAGATATGGTGAATAATGCTCAACGTTCAC
ATGATCGCGTGCCCAGCCCTACCACCTCCGCTCGCAAGAGGCAGAAGACGTCACAATCCATCCC
TTCTGCTTCTGTACCTGTACCATCTCCTGCTGTGCATTCACAGACACTGACTGCCCCAATGCAA
CCATTGTCATCAGCAACAAAAAAAGTAGCTCCACCTGGTACAAAAGGCAAAAAGACTAAGCCAG
GCCAGAAGATACCAGGTGGCTCTGCAATCAAGACCATGTCTTCTGCTGGCCCTAGTGGTAGGGG
ACCAATTATGAATAAGAACCTATCAGGTGGTCTTCCTACTGAACCAATATCAGTAAATCCATTA
ATCGGACGGAAGGTCATGACTCGGTGGCCTGATGATAATAGCTTTTATGAGGCTGTTATAACTG
ACTATGATCCACAGCTGGATCGTTATGCACTGGTTTATGACATAAATACAGCAGATGAAACCTG
GGAGTGGGTTGACTTTAAAGAG
						
					

						Genomic sequence
						
							GAACTGATGATGATCTTCCACCGACATATCAGAACAGAGGTGTAAGAGGCAGTGGACGTGTTAG
TGGTAATGGGAGAGATATTGTTAGCGCTGTTCCATATAATAGAACTAAGCCACAGACAGATATG
GAAACACAAATTCATCAGCTTGAGCAAGATGCATATTGTTCTGTTCTTCGTGCATTTAAAGCAC
AATCTGATGCTATTTCATGGGTAAAACACCACTTAACTCTGTCATTCTTCATCTTCATTTGGTG
TAATCGTTAGCAGTTCTTTTTTCCGCAAAACTGACCAAAAGACAATTTTGAATTCAACTTCTAG
GAAAAGGAAGGTCTTATCACTGAACTTAGAAAGGAACTTAGAGTGTCTGACAAAGAGCACAGGG
AGTTGTTAAACAGAGTGAACGGTGATGATATCATTCAAAGAATAAGGTATAATATTGGGCAGAT
ACCCATTTTTAATGGCACAATGCATCTTTTGTTTTCTTAACTTCAGACATTTTTATTCAAGGGA
ATGGAGAGAGACAAAAGGTGGGCTTCAGGCAGATATGGTGAATAATGCTCAACGTTCACATGAT
CGCGTGCCCAGCCCTACCACCTCCGCTCGCAAGAGGCAGAAGACGTCACAATCCATCCCTTCTG
CTTCTGTACCTGTACCATCTCCTGCTGTGCATTCACAGACACTGACTGCCCCAATGCAACCATT
GTCATCAGCAACAAAAAAAGTAGCTCCACCTGGTACAAAAGGCAAAAAGACTAAGCCAGTAAGT
GATGGTTGAAATCTGCTGAGACAAACCCTAAGACTTCTCTAATTGTGATGTTGTAATATGACCT
TATTTATTTGTTTTTTTTTTTTTTTTGCAATTTTGCTTGAAGGGCCAGAAGATACCAGGTGGCT
CTGCAATCAAGACCATGTCTTCTGCTGGCCCTAGTGGTAGGGGACCAATTATGAATAAGAACCT
ATCAGGTGGTCTTCCTACTGAACCAATATCAGTAAATCCATTAATCGGACGGAAGGTCATGACT
CGGTGGCCTGATGATAATAGCTTTTATGAGGCTGTTATAACTGACTATGATCCACAGCTGGTAT
GTTTTATTCATACGTGATTAAATTTATCTTTGTTTGAAGTACTTCTGATGTCTGTTCTCATTAA
TCATAGGATCGTTATGCACTGGTTTATGACATAAATACAGCAGATGAAACCTGGGAGTGGGTTG
ACTTTAAAGAG
						
					

			
		

Sequences
Splice junction sequence	TACTTCTGATGTCTGTTCTCATTAATCATAGGATCGTTATGCACTGGTTTATGACATAAATACA GCAGATGAAACCTGGGAGTGGGTTGACTTTAAAGAGgaactgatgatgatcttccaccgacata tcagaacagaggtgtaagaggcagtggacgtgttagtggtaatgggagagatattgttagcgct gttccata
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 162 Transcript exons: 14477246-14477374,14478440-14478472 GAACTGATGATGATCTTCCACCGACATATCAGAACAGAGGTGTAAGAGGCAGTGGACGTGTTAG TGGTAATGGGAGAGATATTGTTAGCGCTGTTCCATATAATAGAACTAAGCCACAGACAGATATG GGATGAAACCTGGGAGTGGGTTGACTTTAAAGAG Transcript length: 854 Transcript exons: 14477246-14477457,14477566-14477675,14477755-14478007,14 478120-14478329,14478404-14478472 GAACTGATGATGATCTTCCACCGACATATCAGAACAGAGGTGTAAGAGGCAGTGGACGTGTTAG TGGTAATGGGAGAGATATTGTTAGCGCTGTTCCATATAATAGAACTAAGCCACAGACAGATATG GAAACACAAATTCATCAGCTTGAGCAAGATGCATATTGTTCTGTTCTTCGTGCATTTAAAGCAC AATCTGATGCTATTTCATGGGAAAAGGAAGGTCTTATCACTGAACTTAGAAAGGAACTTAGAGT GTCTGACAAAGAGCACAGGGAGTTGTTAAACAGAGTGAACGGTGATGATATCATTCAAAGAATA AGGGAATGGAGAGAGACAAAAGGTGGGCTTCAGGCAGATATGGTGAATAATGCTCAACGTTCAC ATGATCGCGTGCCCAGCCCTACCACCTCCGCTCGCAAGAGGCAGAAGACGTCACAATCCATCCC TTCTGCTTCTGTACCTGTACCATCTCCTGCTGTGCATTCACAGACACTGACTGCCCCAATGCAA CCATTGTCATCAGCAACAAAAAAAGTAGCTCCACCTGGTACAAAAGGCAAAAAGACTAAGCCAG GCCAGAAGATACCAGGTGGCTCTGCAATCAAGACCATGTCTTCTGCTGGCCCTAGTGGTAGGGG ACCAATTATGAATAAGAACCTATCAGGTGGTCTTCCTACTGAACCAATATCAGTAAATCCATTA ATCGGACGGAAGGTCATGACTCGGTGGCCTGATGATAATAGCTTTTATGAGGCTGTTATAACTG ACTATGATCCACAGCTGGATCGTTATGCACTGGTTTATGACATAAATACAGCAGATGAAACCTG GGAGTGGGTTGACTTTAAAGAG
Genomic sequence	GAACTGATGATGATCTTCCACCGACATATCAGAACAGAGGTGTAAGAGGCAGTGGACGTGTTAG TGGTAATGGGAGAGATATTGTTAGCGCTGTTCCATATAATAGAACTAAGCCACAGACAGATATG GAAACACAAATTCATCAGCTTGAGCAAGATGCATATTGTTCTGTTCTTCGTGCATTTAAAGCAC AATCTGATGCTATTTCATGGGTAAAACACCACTTAACTCTGTCATTCTTCATCTTCATTTGGTG TAATCGTTAGCAGTTCTTTTTTCCGCAAAACTGACCAAAAGACAATTTTGAATTCAACTTCTAG GAAAAGGAAGGTCTTATCACTGAACTTAGAAAGGAACTTAGAGTGTCTGACAAAGAGCACAGGG AGTTGTTAAACAGAGTGAACGGTGATGATATCATTCAAAGAATAAGGTATAATATTGGGCAGAT ACCCATTTTTAATGGCACAATGCATCTTTTGTTTTCTTAACTTCAGACATTTTTATTCAAGGGA ATGGAGAGAGACAAAAGGTGGGCTTCAGGCAGATATGGTGAATAATGCTCAACGTTCACATGAT CGCGTGCCCAGCCCTACCACCTCCGCTCGCAAGAGGCAGAAGACGTCACAATCCATCCCTTCTG CTTCTGTACCTGTACCATCTCCTGCTGTGCATTCACAGACACTGACTGCCCCAATGCAACCATT GTCATCAGCAACAAAAAAAGTAGCTCCACCTGGTACAAAAGGCAAAAAGACTAAGCCAGTAAGT GATGGTTGAAATCTGCTGAGACAAACCCTAAGACTTCTCTAATTGTGATGTTGTAATATGACCT TATTTATTTGTTTTTTTTTTTTTTTTGCAATTTTGCTTGAAGGGCCAGAAGATACCAGGTGGCT CTGCAATCAAGACCATGTCTTCTGCTGGCCCTAGTGGTAGGGGACCAATTATGAATAAGAACCT ATCAGGTGGTCTTCCTACTGAACCAATATCAGTAAATCCATTAATCGGACGGAAGGTCATGACT CGGTGGCCTGATGATAATAGCTTTTATGAGGCTGTTATAACTGACTATGATCCACAGCTGGTAT GTTTTATTCATACGTGATTAAATTTATCTTTGTTTGAAGTACTTCTGATGTCTGTTCTCATTAA TCATAGGATCGTTATGCACTGGTTTATGACATAAATACAGCAGATGAAACCTGGGAGTGGGTTG ACTTTAAAGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_007674* osi_circ_017796 zma_circ_008041* zma_circ_001688 
						
						PMCS
						0.259755712

Conservation Information
Conserved circRNAs	osi_circ_007674* osi_circ_017796 zma_circ_008041* zma_circ_001688
PMCS	0.259755712

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							14477372-14477287(+)
14477390-14478423(-)
14477274-14478248(-)
						
						Potential amino acid sequence
						
							METQIHQLEQDAYCSVLRAFKAQSDAISWEKEGLITELRKELRVSDKEHRELLNRVNGDDIIQR
IREWRETKGGLQADMVNNAQRSHDRVPSPTTSARKRQKTSQSIPSASVPVPSPAVHSQTLTAPM
QPLSSATKKVAPPGTKGKKTKPGQKIPGGSAIKTMSSAGPSGRGPIMNKNLSGGLPTEPISVNP
LIGRKVMTRWPDDNSFYEAVITDYDPQLDRYALVYDINTADETWEWVDFKEELMMIFHRHIRTE
V*(+)
MNLCFHICLWLSSIIWNSANNISPITTNTSTASYTSVLICRWKIIISSSLKSTHSQVSSAVFMS
*(-)
MSVEDHHQFLFKVNPLPGFICCIYVINQCITIQLWIIVSYNSLIKAIIIRPPSHDLPSD*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	14477372-14477287(+) 14477390-14478423(-) 14477274-14478248(-)
Potential amino acid sequence	METQIHQLEQDAYCSVLRAFKAQSDAISWEKEGLITELRKELRVSDKEHRELLNRVNGDDIIQR IREWRETKGGLQADMVNNAQRSHDRVPSPTTSARKRQKTSQSIPSASVPVPSPAVHSQTLTAPM QPLSSATKKVAPPGTKGKKTKPGQKIPGGSAIKTMSSAGPSGRGPIMNKNLSGGLPTEPISVNP LIGRKVMTRWPDDNSFYEAVITDYDPQLDRYALVYDINTADETWEWVDFKEELMMIFHRHIRTE V(+) MNLCFHICLWLSSIIWNSANNISPITTNTSTASYTSVLICRWKIIISSSLKSTHSQVSSAVFMS (-) MSVEDHHQFLFKVNPLPGFICCIYVINQCITIQLWIIVSYNSLIKAIIIRPPSHDLPSD*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os08g0328300_circ_g.1

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