Plant Circular RNA Database

Detail information of Os03g0328900_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os03g0328900_circ_g.3
					
						ID in PlantcircBase
						osa_circ_019616
					
						Alias
						Os_ciR5327
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 12049111-12049586  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os03g0328900						
					
						Parent gene annotation
						
							Similar to cDNA clone:J033099F01, full insert sequence. (Os03t03
28900-01);Nucleotide-binding, alpha-beta plait domain containing
 protein. (Os03t0328900-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os03g0328900_circ_ag.1 Os03g0328900_circ_g.1 Os03g0328900_circ_ag.2 Os03g0328900_circ_g.4 						
					
						Support reads
						3/1
					
						Tissues
						root/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0328900-01:2
Os03t0328900-02:2

General Information
CircRNA Name	Os03g0328900_circ_g.3
ID in PlantcircBase	osa_circ_019616
Alias	Os_ciR5327
Organism	Oryza sativa
Position	chr3: 12049111-12049586 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os03g0328900
Parent gene annotation	Similar to cDNA clone:J033099F01, full insert sequence. (Os03t03 28900-01);Nucleotide-binding, alpha-beta plait domain containing protein. (Os03t0328900-02)
Parent gene strand	+
Alternative splicing	Os03g0328900_circ_ag.1 Os03g0328900_circ_g.1 Os03g0328900_circ_ag.2 Os03g0328900_circ_g.4
Support reads	3/1
Tissues	root/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0328900-01:2 Os03t0328900-02:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GAACAGGTTACTTCACAGAAGAGAGGAAAATGGTCAACGGAGGAGGTGATAAGGAAGAATCTGC
TGGTGAGGCGAGCCTGAATGCTGATAGCGACCAAAGgtgcgaggatgctgcagcactccaatag
cacaaacgagatgatgcttcgtaggaaactggaggagcagcagcaggctgccgagctgcaacag
gccatcga

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 376
Transcript exons: 12049111-12049371,12049472-12049586
GTGCGAGGATGCTGCAGCACTCCAATAGCACAAACGAGATGATGCTTCGTAGGAAACTGGAGGA
GCAGCAGCAGGCTGCCGAGCTGCAACAGGCCATCGAACTCCACAGTCGCCGTCTCATGGACCTC
CAGCTGCTCGACCTCAAGAATAGAGCCGCAGCTGCTGTCACAACAGCAATGGCGATGACAATTC
CCACCGCCAATGCCTTCGGTTCCAGTCAGCCTCTTGCCACCACCATGGTCGAGTCACCGCCTGA
TTCTGGCGAGCAGCTCAAGGGAACAGGTTACTTCACAGAAGAGAGGAAAATGGTCAACGGAGGA
GGTGATAAGGAAGAATCTGCTGGTGAGGCGAGCCTGAATGCTGATAGCGACCAAAG
						
						Genomic sequence
						
							GTGCGAGGATGCTGCAGCACTCCAATAGCACAAACGAGATGATGCTTCGTAGGAAACTGGAGGA
GCAGCAGCAGGCTGCCGAGCTGCAACAGGCCATCGAACTCCACAGTCGCCGTCTCATGGACCTC
CAGCTGCTCGACCTCAAGAATAGAGCCGCAGCTGCTGTCACAACAGCAATGGCGATGACAATTC
CCACCGCCAATGCCTTCGGTTCCAGTCAGCCTCTTGCCACCACCATGGTCGAGTCACCGCCTGA
TTCTGGTATGTGTTACTTCGGTCGGTCTGCCTGTCAAGCATTGTAAGACGAGTTCATCCACTCA
TTCGACACTACTGATCCTTGTAATTTCTTGGTCGTTTATAGGCGAGCAGCTCAAGGGAACAGGT
TACTTCACAGAAGAGAGGAAAATGGTCAACGGAGGAGGTGATAAGGAAGAATCTGCTGGTGAGG
CGAGCCTGAATGCTGATAGCGACCAAAG

Sequences
Splice junction sequence	GAACAGGTTACTTCACAGAAGAGAGGAAAATGGTCAACGGAGGAGGTGATAAGGAAGAATCTGC TGGTGAGGCGAGCCTGAATGCTGATAGCGACCAAAGgtgcgaggatgctgcagcactccaatag cacaaacgagatgatgcttcgtaggaaactggaggagcagcagcaggctgccgagctgcaacag gccatcga
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 376 Transcript exons: 12049111-12049371,12049472-12049586 GTGCGAGGATGCTGCAGCACTCCAATAGCACAAACGAGATGATGCTTCGTAGGAAACTGGAGGA GCAGCAGCAGGCTGCCGAGCTGCAACAGGCCATCGAACTCCACAGTCGCCGTCTCATGGACCTC CAGCTGCTCGACCTCAAGAATAGAGCCGCAGCTGCTGTCACAACAGCAATGGCGATGACAATTC CCACCGCCAATGCCTTCGGTTCCAGTCAGCCTCTTGCCACCACCATGGTCGAGTCACCGCCTGA TTCTGGCGAGCAGCTCAAGGGAACAGGTTACTTCACAGAAGAGAGGAAAATGGTCAACGGAGGA GGTGATAAGGAAGAATCTGCTGGTGAGGCGAGCCTGAATGCTGATAGCGACCAAAG
Genomic sequence	GTGCGAGGATGCTGCAGCACTCCAATAGCACAAACGAGATGATGCTTCGTAGGAAACTGGAGGA GCAGCAGCAGGCTGCCGAGCTGCAACAGGCCATCGAACTCCACAGTCGCCGTCTCATGGACCTC CAGCTGCTCGACCTCAAGAATAGAGCCGCAGCTGCTGTCACAACAGCAATGGCGATGACAATTC CCACCGCCAATGCCTTCGGTTCCAGTCAGCCTCTTGCCACCACCATGGTCGAGTCACCGCCTGA TTCTGGTATGTGTTACTTCGGTCGGTCTGCCTGTCAAGCATTGTAAGACGAGTTCATCCACTCA TTCGACACTACTGATCCTTGTAATTTCTTGGTCGTTTATAGGCGAGCAGCTCAAGGGAACAGGT TACTTCACAGAAGAGAGGAAAATGGTCAACGGAGGAGGTGATAAGGAAGAATCTGCTGGTGAGG CGAGCCTGAATGCTGATAGCGACCAAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_012963 						
					
						PMCS
						0.47671285

Conservation Information
Conserved circRNAs	osi_circ_012963
PMCS	0.47671285

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							12049569-12049135(+)
12049120-12049135(+)
12049233-12049358(-)
						
						Potential amino acid sequence
						
							MLIATKGARMLQHSNSTNEMMLRRKLEEQQQAAELQQAIELHSRRLMDLQLLDLKNRAAAAVTT
AMAMTIPTANAFGSSQPLATTMVESPPDSGEQLKGTGYFTEERKMVNGGGDKEESAGEASLNAD
SDQRCEDAAALQ*(+)
MLQHSNSTNEMMLRRKLEEQQQAAELQQAIELHSRRLMDLQLLDLKNRAAAAVTTAMAMTIPTA
NAFGSSQPLATTMVESPPDSGEQLKGTGYFTEERKMVNGGGDKEESAGEASLNADSDQRCEDAA
ALQ*(+)
MRRRLWSSMACCSSAACCCSSSFLRSIISFVLLECCSILAPLVAISIQARLTSRFFLITSSVDH
FPLFCEVTCSLELLARIRR*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	12049569-12049135(+) 12049120-12049135(+) 12049233-12049358(-)
Potential amino acid sequence	MLIATKGARMLQHSNSTNEMMLRRKLEEQQQAAELQQAIELHSRRLMDLQLLDLKNRAAAAVTT AMAMTIPTANAFGSSQPLATTMVESPPDSGEQLKGTGYFTEERKMVNGGGDKEESAGEASLNAD SDQRCEDAAALQ(+) MLQHSNSTNEMMLRRKLEEQQQAAELQQAIELHSRRLMDLQLLDLKNRAAAAVTTAMAMTIPTA NAFGSSQPLATTMVESPPDSGEQLKGTGYFTEERKMVNGGGDKEESAGEASLNADSDQRCEDAA ALQ(+) MRRRLWSSMACCSSAACCCSSSFLRSIISFVLLECCSILAPLVAISIQARLTSRFFLITSSVDH FPLFCEVTCSLELLARIRR*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os03g0328900_circ_g.3

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