Plant Circular RNA Database

Detail information of orai.004G215900_circ_g.1

			
						 General Information
					
						CircRNA Name
						orai.004G215900_circ_g.1
					
						ID in PlantcircBase
						gra_circ_000388
					
						Alias
						Chr04:54901301|54902385
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr04: 54901301-54902385  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.004G215900						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						2/17
					
						Tissues
						leaf/ovule
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.004G215900.3:4
Gorai.004G215900.4:4
Gorai.004G215900.2:3
Gorai.004G215900.1:4

General Information
CircRNA Name	orai.004G215900_circ_g.1
ID in PlantcircBase	gra_circ_000388
Alias	Chr04:54901301\|54902385
Organism	Gossypium raimondii
Position	chrChr04: 54901301-54902385 JBrowse»
Reference genome	Graimondii_221
Type	e-circRNA
Identification method	CIRI
Parent gene	Gorai.004G215900
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	NA
Support reads	2/17
Tissues	leaf/ovule
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.004G215900.3:4 Gorai.004G215900.4:4 Gorai.004G215900.2:3 Gorai.004G215900.1:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GACAATCAACGGCGAGCTGAGTTGTTTAGAGATGAGTCGAATCGGAGGTGGCAAATGCTACAAG
AACCCTCGTGCGGTAGTCTTCAACCACTTTCAACAGgttacctggaaaggcatatgcaaggagg
caacatgtatggcttacaggtgcaaacgaagttgatagcaaaacattttcaagagctattcttg
ccaaggta

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 752
Transcript exons: 54901301-54901397,54901544-54901558,54901672-54901728,54
901803-54902385
GTTACCTGGAAAGGCATATGCAAGGAGGCAACATGTATGGCTTACAGGTGCAAACGAAGTTGAT
AGCAAAACATTTTCAAGAGCTATTCTTGCCAAGAGTGCTTGTATACAGACTGTGCTTTGCATTC
CTATTCTTGATGGTGTTCTTGAACTTGGATATACCGAAAAGGTGCAAGAAGATTTAGGGTTAGT
CCAGCACGTTAAAACTTTCTTCAACGATGGAAAAGCACCAAACCCTCCGCCACCAAAACCGGCT
CTCTCCGAACATTCAACGTCGAACCCGGCCGCTTCATCGGACTACACTCGATTTCATTCCCCTT
CCATTCCTCTCGTTTACGCAGCAGCGGACCCACCGGTGAACGCCGACCAAGGAGAGTATAACAA
CGTTGATGAAGAGGAGGAGGAAGAAGAAGAAGATGACGACGATGAAGAGGGAGACGAAGAACCC
GAATCGTACTCGGCCGAAACGGGTCGAACCACGCAACAAGTCCCGACGGAAAACACTCTGGCAG
TAGTGGCGGCGGAGGAGCCGAGCGAGTTAATGCAATTGGATATGTCGGAAGATATCAAGCTCGG
TTCACCTGACGATGCATCGAATAACTTGGACTCGGATTTCAATATGTTGGCGGTGAGTCAAAGC
GGGAACCCAACCGACAATCAACGGCGAGCTGAGTTGTTTAGAGATGAGTCGAATCGGAGGTGGC
AAATGCTACAAGAACCCTCGTGCGGTAGTCTTCAACCACTTTCAACAG
						
					

						Genomic sequence
						
							GTTACCTGGAAAGGCATATGCAAGGAGGCAACATGTATGGCTTACAGGTGCAAACGAAGTTGAT
AGCAAAACATTTTCAAGAGCTATTCTTGCCAAGGTATATGGATATACACATATATATATATATA
TATGTATATATCTTGTTCTTGATTTGCATGTTTTTAACTGCAATATTTCAATGTACTAACTGTA
TTCATGTCTTCGTGTTTGTCAACATAAAATAATGCCGTACGGTTGCAACAGAGTGCTTGTATAC
AGGTATCTATATACATATACATATGTATGAATGTATGTATGTATCATTTTAACTCATGACGATG
AAATTAAACCATTTTTTTATTTTTTTTCTTTTGGTTTTTTGTTGTTTGTAGACTGTGCTTTGCA
TTCCTATTCTTGATGGTGTTCTTGAACTTGGATATACCGAAAAGGTAATTATAAGTATTATTAA
AAGAGGTAATTACGAATTTGAAAAAAGTGGAATACCGATTTAATTATATTGTAGGTGCAAGAAG
ATTTAGGGTTAGTCCAGCACGTTAAAACTTTCTTCAACGATGGAAAAGCACCAAACCCTCCGCC
ACCAAAACCGGCTCTCTCCGAACATTCAACGTCGAACCCGGCCGCTTCATCGGACTACACTCGA
TTTCATTCCCCTTCCATTCCTCTCGTTTACGCAGCAGCGGACCCACCGGTGAACGCCGACCAAG
GAGAGTATAACAACGTTGATGAAGAGGAGGAGGAAGAAGAAGAAGATGACGACGATGAAGAGGG
AGACGAAGAACCCGAATCGTACTCGGCCGAAACGGGTCGAACCACGCAACAAGTCCCGACGGAA
AACACTCTGGCAGTAGTGGCGGCGGAGGAGCCGAGCGAGTTAATGCAATTGGATATGTCGGAAG
ATATCAAGCTCGGTTCACCTGACGATGCATCGAATAACTTGGACTCGGATTTCAATATGTTGGC
GGTGAGTCAAAGCGGGAACCCAACCGACAATCAACGGCGAGCTGAGTTGTTTAGAGATGAGTCG
AATCGGAGGTGGCAAATGCTACAAGAACCCTCGTGCGGTAGTCTTCAACCACTTTCAACAG
						
					

			
		

Sequences
Splice junction sequence	GACAATCAACGGCGAGCTGAGTTGTTTAGAGATGAGTCGAATCGGAGGTGGCAAATGCTACAAG AACCCTCGTGCGGTAGTCTTCAACCACTTTCAACAGgttacctggaaaggcatatgcaaggagg caacatgtatggcttacaggtgcaaacgaagttgatagcaaaacattttcaagagctattcttg ccaaggta
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 752 Transcript exons: 54901301-54901397,54901544-54901558,54901672-54901728,54 901803-54902385 GTTACCTGGAAAGGCATATGCAAGGAGGCAACATGTATGGCTTACAGGTGCAAACGAAGTTGAT AGCAAAACATTTTCAAGAGCTATTCTTGCCAAGAGTGCTTGTATACAGACTGTGCTTTGCATTC CTATTCTTGATGGTGTTCTTGAACTTGGATATACCGAAAAGGTGCAAGAAGATTTAGGGTTAGT CCAGCACGTTAAAACTTTCTTCAACGATGGAAAAGCACCAAACCCTCCGCCACCAAAACCGGCT CTCTCCGAACATTCAACGTCGAACCCGGCCGCTTCATCGGACTACACTCGATTTCATTCCCCTT CCATTCCTCTCGTTTACGCAGCAGCGGACCCACCGGTGAACGCCGACCAAGGAGAGTATAACAA CGTTGATGAAGAGGAGGAGGAAGAAGAAGAAGATGACGACGATGAAGAGGGAGACGAAGAACCC GAATCGTACTCGGCCGAAACGGGTCGAACCACGCAACAAGTCCCGACGGAAAACACTCTGGCAG TAGTGGCGGCGGAGGAGCCGAGCGAGTTAATGCAATTGGATATGTCGGAAGATATCAAGCTCGG TTCACCTGACGATGCATCGAATAACTTGGACTCGGATTTCAATATGTTGGCGGTGAGTCAAAGC GGGAACCCAACCGACAATCAACGGCGAGCTGAGTTGTTTAGAGATGAGTCGAATCGGAGGTGGC AAATGCTACAAGAACCCTCGTGCGGTAGTCTTCAACCACTTTCAACAG
Genomic sequence	GTTACCTGGAAAGGCATATGCAAGGAGGCAACATGTATGGCTTACAGGTGCAAACGAAGTTGAT AGCAAAACATTTTCAAGAGCTATTCTTGCCAAGGTATATGGATATACACATATATATATATATA TATGTATATATCTTGTTCTTGATTTGCATGTTTTTAACTGCAATATTTCAATGTACTAACTGTA TTCATGTCTTCGTGTTTGTCAACATAAAATAATGCCGTACGGTTGCAACAGAGTGCTTGTATAC AGGTATCTATATACATATACATATGTATGAATGTATGTATGTATCATTTTAACTCATGACGATG AAATTAAACCATTTTTTTATTTTTTTTCTTTTGGTTTTTTGTTGTTTGTAGACTGTGCTTTGCA TTCCTATTCTTGATGGTGTTCTTGAACTTGGATATACCGAAAAGGTAATTATAAGTATTATTAA AAGAGGTAATTACGAATTTGAAAAAAGTGGAATACCGATTTAATTATATTGTAGGTGCAAGAAG ATTTAGGGTTAGTCCAGCACGTTAAAACTTTCTTCAACGATGGAAAAGCACCAAACCCTCCGCC ACCAAAACCGGCTCTCTCCGAACATTCAACGTCGAACCCGGCCGCTTCATCGGACTACACTCGA TTTCATTCCCCTTCCATTCCTCTCGTTTACGCAGCAGCGGACCCACCGGTGAACGCCGACCAAG GAGAGTATAACAACGTTGATGAAGAGGAGGAGGAAGAAGAAGAAGATGACGACGATGAAGAGGG AGACGAAGAACCCGAATCGTACTCGGCCGAAACGGGTCGAACCACGCAACAAGTCCCGACGGAA AACACTCTGGCAGTAGTGGCGGCGGAGGAGCCGAGCGAGTTAATGCAATTGGATATGTCGGAAG ATATCAAGCTCGGTTCACCTGACGATGCATCGAATAACTTGGACTCGGATTTCAATATGTTGGC GGTGAGTCAAAGCGGGAACCCAACCGACAATCAACGGCGAGCTGAGTTGTTTAGAGATGAGTCG AATCGGAGGTGGCAAATGCTACAAGAACCCTCGTGCGGTAGTCTTCAACCACTTTCAACAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							gar_circ_000553 						
					
						PMCS
						1.128751889

Conservation Information
Conserved circRNAs	gar_circ_000553
PMCS	1.128751889

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							54902175-54901815(+)
54902317-54901360(+)
54901686-54902313(-)
						
						Potential amino acid sequence
						
							MQLDMSEDIKLGSPDDASNNLDSDFNMLAVSQSGNPTDNQRRAELFRDESNRRWQMLQEPSCGS
LQPLSTGYLERHMQGGNMYGLQVQTKLIAKHFQELFLPRVLVYRLCFAFLFLMVFLNLDIPKRC
KKI*(+)
MSRIGGGKCYKNPRAVVFNHFQQVTWKGICKEATCMAYRCKRS*(+)
MQSTVCIQALLARIALENVLLSTSFAPVSHTCCLLAYAFPGNLLKVVEDYRTRVLVAFATSDST
HL*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	54902175-54901815(+) 54902317-54901360(+) 54901686-54902313(-)
Potential amino acid sequence	MQLDMSEDIKLGSPDDASNNLDSDFNMLAVSQSGNPTDNQRRAELFRDESNRRWQMLQEPSCGS LQPLSTGYLERHMQGGNMYGLQVQTKLIAKHFQELFLPRVLVYRLCFAFLFLMVFLNLDIPKRC KKI(+) MSRIGGGKCYKNPRAVVFNHFQQVTWKGICKEATCMAYRCKRS(+) MQSTVCIQALLARIALENVLLSTSFAPVSHTCCLLAYAFPGNLLKVVEDYRTRVLVAFATSDST HL*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.004G215900_circ_g.1

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