Plant Circular RNA Database

Detail information of Os08g0342400_circ_g.5

			
						 General Information
					
						CircRNA Name
						Os08g0342400_circ_g.5
					
						ID in PlantcircBase
						osa_circ_036795
					
						Alias
						Os08circ07181/Os_ciR1516
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr8: 15447715-15449688  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, SMALT, Segemehl, find_circ
					
						Parent gene
						
							Os08g0342400						
					
						Parent gene annotation
						
							Similar to aspartate kinase-homoserine dehydrogenase. (Os08t0342
400-01);Similar to Bifunctional aspartokinase/homoserine dehydro
genase 1, chloroplast precursor (AK-HD 1) (AK-HSDH 1) [Includes:
 Aspartokinase (EC 2.7.2.4); Homoserine dehydrogenase (EC 1.1.1.
3)]. (Os08t0342400-02);Similar to Bifunctional aspartokinase/hom
oserine dehydrogenase 1, chloroplast precursor (AK-HD 1) (AK-HSD
H 1) [Includes: Aspartokinase (EC 2.7.2.4); Homoserine dehydroge
nase (EC 1.1.1.3)]. (Os08t0342400-03)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os08g0342400_circ_g.1 Os08g0342400_circ_g.2 Os08g0342400_circ_g.3 Os08g0342400_circ_g.4 Os08g0342400_circ_g.6 						
					
						Support reads
						2/8/4
					
						Tissues
						leaf and panicle/root/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os08t0342400-03:3
Os08t0342400-01:4
Os08t0342400-02:4

General Information
CircRNA Name	Os08g0342400_circ_g.5
ID in PlantcircBase	osa_circ_036795
Alias	Os08circ07181/Os_ciR1516
Organism	Oryza sativa
Position	chr8: 15447715-15449688 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, SMALT, Segemehl, find_circ
Parent gene	Os08g0342400
Parent gene annotation	Similar to aspartate kinase-homoserine dehydrogenase. (Os08t0342 400-01);Similar to Bifunctional aspartokinase/homoserine dehydro genase 1, chloroplast precursor (AK-HD 1) (AK-HSDH 1) [Includes: Aspartokinase (EC 2.7.2.4); Homoserine dehydrogenase (EC 1.1.1. 3)]. (Os08t0342400-02);Similar to Bifunctional aspartokinase/hom oserine dehydrogenase 1, chloroplast precursor (AK-HD 1) (AK-HSD H 1) [Includes: Aspartokinase (EC 2.7.2.4); Homoserine dehydroge nase (EC 1.1.1.3)]. (Os08t0342400-03)
Parent gene strand	-
Alternative splicing	Os08g0342400_circ_g.1 Os08g0342400_circ_g.2 Os08g0342400_circ_g.3 Os08g0342400_circ_g.4 Os08g0342400_circ_g.6
Support reads	2/8/4
Tissues	leaf and panicle/root/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os08t0342400-03:3 Os08t0342400-01:4 Os08t0342400-02:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							AAACAATGGCCATGAAAGATGATAGATAATATTACCAGCAGCTGGCTTGAGCATATTCTTCGAA
ACATCCCCATAAAGTCCTCCTGGAAGGTCGCTGGCCcttttctgggatctgcactaaagacccc
atcaacatcagtccagatagtgacctgaccagccttaacaagtgaaccaattatggctgcagag
aaatcact

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 833
Transcript exons: 15447715-15448018,15448520-15448608,15448826-15449200,15
449624-15449688
CTTTTCTGGGATCTGCACTAAAGACCCCATCAACATCAGTCCAGATAGTGACCTGACCAGCCTT
AACAAGTGAACCAATTATGGCTGCAGAGAAATCACTTCCATCTCTTTTCAAGGTTGTAGGAATG
TTTTCAGGTGTACTCGCAATAAACCCAGTAGCAATTATTGTCTCAGCTGGCTGTCGAGCGAACC
ATTTCTCAAGTCGTTTCTCAGATTCCAAATAGTCAGGATCTACTTGGTTGGAACCTGTAGGATT
TACAACTAAAACTTCTCGTGTGTCCATCCAACTGCAAGGGGTTCCAGACTTCTTTATGGCAAAT
GACAACAATTGAGCTGACCATATCTCTCCATGTCCAACAACAAAATCTGAGAAAGACTCCGTGG
CATGCCCAGCTATGCAAATTGCACGAAGCATTGCTTTTAGGTTACTGACATCCGCGTGCAACTG
AGATAAGAATCTTGCAAGGTCCTCTCCTCCAAGCAGATCCTTTGCTGCTGCCATATGCTTCTCG
AAAACTTCATCAAGTGCAGTGATATAAGAATCATCCCTTGATTGAGCCTTATTTACAAGGTTGT
ACATCATGTCAGTGACTTTAGACATTGCAGACACAACTACCAGTTTCCTCTCAGAAGGGTCGCG
AAGGATTATGTCAGCCACATTCTGAATCCTCTGGGAGGTTCCCATGCAGGTCCCACCAAATTTA
TGCACAGACCACATGTCACCTTTTGGAAGGTGAGCACTAGCTTCAGCTTGCTCAACTGAAACAG
CAGCAGCTGGCTTGAGCATATTCTTCGAAACATCCCCATAAAGTCCTCCTGGAAGGTCGCTGGC
C
						
					

						Genomic sequence
						
							CTTTTCTGGGATCTGCACTAAAGACCCCATCAACATCAGTCCAGATAGTGACCTGACCAGCCTT
AACAAGTGAACCAATTATGGCTGCAGAGAAATCACTTCCATCTCTTTTCAAGGTTGTAGGAATG
TTTTCAGGTGTACTCGCAATAAACCCAGTAGCAATTATTGTCTCAGCTGGCTGTCGAGCGAACC
ATTTCTCAAGTCGTTTCTCAGATTCCAAATAGTCAGGATCTACTTGGTTGGAACCTGTAGGATT
TACAACTAAAACTTCTCGTGTGTCCATCCAACTGCAAGGGGTTCCAGACTGCATAGTGTAAGAT
ATTTTGGTGTAAGTCCATGTTTTTAAAGGTATAAGCTAGGTAATATTTTTATATGAGCAGTGCC
TTCTGAATATAGTAGGTGTATTTCCATAGAGATTACTCTAAACTAAATGCAAATGCACAAATTG
GTTTTGTTGATAATAACAACATTAAAGAACCAAATGGTTTGGTACAAGTGTAAAACTACACCCA
AATATTTAAAATAAGCTCCTATGCACTTATAAAATAGAATATTTATTTCTCATGAATAATAATG
TTCAGAAAAGGAGATCGAGCACTGCTAGTTCTATCTCAAAAAAGAAAGGTGCATATAGACTACA
TTCAGCTGTGGCTTGACCGGATTTCCACAGAAGAAAGGATGATCCAATGAGCAATTATTACTGA
AGCTACTGTAGTGTAAGGAGGTTCTATGCATGAATTCTACAGCGAGAGTAATTGCTTTGTGCCA
TAGAGATGTTAATTTCTTTTAGTGTGTTCATTGTTACCTTCTTTATGGCAAATGACAACAATTG
AGCTGACCATATCTCTCCATGTCCAACAACAAAATCTGAGAAAGACTCCGTGGCATGCCCAGCT
GCAAAAAGGGAAATGTATCATAACTCAGCTAAGACTATATATCTGCAATCAAACCACACCATTG
GTACATAATTGTAAATAGTCAGCAGTTAGCACAGAAGTACTAAGGGCCCATGTAAATCCTCTCC
ATAAGTTGGACAAAATAAAGGACAACCTATTTTACTGAAAAAGAAAAACATATGACAGAAAGAT
AAGAAAAATAGGGTGTGATTAACCTATGCAAATTGCACGAAGCATTGCTTTTAGGTTACTGACA
TCCGCGTGCAACTGAGATAAGAATCTTGCAAGGTCCTCTCCTCCAAGCAGATCCTTTGCTGCTG
CCATATGCTTCTCGAAAACTTCATCAAGTGCAGTGATATAAGAATCATCCCTTGATTGAGCCTT
ATTTACAAGGTTGTACATCATGTCAGTGACTTTAGACATTGCAGACACAACTACCAGTTTCCTC
TCAGAAGGGTCGCGAAGGATTATGTCAGCCACATTCTGAATCCTCTGGGAGGTTCCCATGCAGG
TCCCACCAAATTTATGCACAGACCACATGTCACCTTTTGGAAGGTGAGCACTAGCTTCAGCTTG
CTCAACTGAAACAGCTGAGAAGAAATTCATAAACTTAGATGGCAATATATTAATGATCCACTTC
TATCAATACAATGAACGAACTATTTATTCCTATTTTTTGTTATCTTAATGAGACAAAGACAAGC
CTTCTTTACCACTTTAGGGGAAGTAAAGCTAAAAGAAAAATGTGATAGTAACAATAACATTGAT
AGTGGTTAAATTGTATCAAAATCAAAACCTGAGATGCAATACCAAAAATGAGAATAAGTACCCT
GCAATAGCTTTAATCAAAGAACAGTTCCAATTGAGTAATGGAATCTAGCCTATGGAATTTCTCC
GTCAAAAAAGGGGTGAAAATTATACCACAATCTAGACTAGACTAAAAGTGAACTGGGAGAAAGG
AACAAATTCAACATAGTGAAACAATGGCCATGAAAGATGATAGATAATATTACCAGCAGCTGGC
TTGAGCATATTCTTCGAAACATCCCCATAAAGTCCTCCTGGAAGGTCGCTGGCC
						
					

			
		

Sequences
Splice junction sequence	AAACAATGGCCATGAAAGATGATAGATAATATTACCAGCAGCTGGCTTGAGCATATTCTTCGAA ACATCCCCATAAAGTCCTCCTGGAAGGTCGCTGGCCcttttctgggatctgcactaaagacccc atcaacatcagtccagatagtgacctgaccagccttaacaagtgaaccaattatggctgcagag aaatcact
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 833 Transcript exons: 15447715-15448018,15448520-15448608,15448826-15449200,15 449624-15449688 CTTTTCTGGGATCTGCACTAAAGACCCCATCAACATCAGTCCAGATAGTGACCTGACCAGCCTT AACAAGTGAACCAATTATGGCTGCAGAGAAATCACTTCCATCTCTTTTCAAGGTTGTAGGAATG TTTTCAGGTGTACTCGCAATAAACCCAGTAGCAATTATTGTCTCAGCTGGCTGTCGAGCGAACC ATTTCTCAAGTCGTTTCTCAGATTCCAAATAGTCAGGATCTACTTGGTTGGAACCTGTAGGATT TACAACTAAAACTTCTCGTGTGTCCATCCAACTGCAAGGGGTTCCAGACTTCTTTATGGCAAAT GACAACAATTGAGCTGACCATATCTCTCCATGTCCAACAACAAAATCTGAGAAAGACTCCGTGG CATGCCCAGCTATGCAAATTGCACGAAGCATTGCTTTTAGGTTACTGACATCCGCGTGCAACTG AGATAAGAATCTTGCAAGGTCCTCTCCTCCAAGCAGATCCTTTGCTGCTGCCATATGCTTCTCG AAAACTTCATCAAGTGCAGTGATATAAGAATCATCCCTTGATTGAGCCTTATTTACAAGGTTGT ACATCATGTCAGTGACTTTAGACATTGCAGACACAACTACCAGTTTCCTCTCAGAAGGGTCGCG AAGGATTATGTCAGCCACATTCTGAATCCTCTGGGAGGTTCCCATGCAGGTCCCACCAAATTTA TGCACAGACCACATGTCACCTTTTGGAAGGTGAGCACTAGCTTCAGCTTGCTCAACTGAAACAG CAGCAGCTGGCTTGAGCATATTCTTCGAAACATCCCCATAAAGTCCTCCTGGAAGGTCGCTGGC C
Genomic sequence	CTTTTCTGGGATCTGCACTAAAGACCCCATCAACATCAGTCCAGATAGTGACCTGACCAGCCTT AACAAGTGAACCAATTATGGCTGCAGAGAAATCACTTCCATCTCTTTTCAAGGTTGTAGGAATG TTTTCAGGTGTACTCGCAATAAACCCAGTAGCAATTATTGTCTCAGCTGGCTGTCGAGCGAACC ATTTCTCAAGTCGTTTCTCAGATTCCAAATAGTCAGGATCTACTTGGTTGGAACCTGTAGGATT TACAACTAAAACTTCTCGTGTGTCCATCCAACTGCAAGGGGTTCCAGACTGCATAGTGTAAGAT ATTTTGGTGTAAGTCCATGTTTTTAAAGGTATAAGCTAGGTAATATTTTTATATGAGCAGTGCC TTCTGAATATAGTAGGTGTATTTCCATAGAGATTACTCTAAACTAAATGCAAATGCACAAATTG GTTTTGTTGATAATAACAACATTAAAGAACCAAATGGTTTGGTACAAGTGTAAAACTACACCCA AATATTTAAAATAAGCTCCTATGCACTTATAAAATAGAATATTTATTTCTCATGAATAATAATG TTCAGAAAAGGAGATCGAGCACTGCTAGTTCTATCTCAAAAAAGAAAGGTGCATATAGACTACA TTCAGCTGTGGCTTGACCGGATTTCCACAGAAGAAAGGATGATCCAATGAGCAATTATTACTGA AGCTACTGTAGTGTAAGGAGGTTCTATGCATGAATTCTACAGCGAGAGTAATTGCTTTGTGCCA TAGAGATGTTAATTTCTTTTAGTGTGTTCATTGTTACCTTCTTTATGGCAAATGACAACAATTG AGCTGACCATATCTCTCCATGTCCAACAACAAAATCTGAGAAAGACTCCGTGGCATGCCCAGCT GCAAAAAGGGAAATGTATCATAACTCAGCTAAGACTATATATCTGCAATCAAACCACACCATTG GTACATAATTGTAAATAGTCAGCAGTTAGCACAGAAGTACTAAGGGCCCATGTAAATCCTCTCC ATAAGTTGGACAAAATAAAGGACAACCTATTTTACTGAAAAAGAAAAACATATGACAGAAAGAT AAGAAAAATAGGGTGTGATTAACCTATGCAAATTGCACGAAGCATTGCTTTTAGGTTACTGACA TCCGCGTGCAACTGAGATAAGAATCTTGCAAGGTCCTCTCCTCCAAGCAGATCCTTTGCTGCTG CCATATGCTTCTCGAAAACTTCATCAAGTGCAGTGATATAAGAATCATCCCTTGATTGAGCCTT ATTTACAAGGTTGTACATCATGTCAGTGACTTTAGACATTGCAGACACAACTACCAGTTTCCTC TCAGAAGGGTCGCGAAGGATTATGTCAGCCACATTCTGAATCCTCTGGGAGGTTCCCATGCAGG TCCCACCAAATTTATGCACAGACCACATGTCACCTTTTGGAAGGTGAGCACTAGCTTCAGCTTG CTCAACTGAAACAGCTGAGAAGAAATTCATAAACTTAGATGGCAATATATTAATGATCCACTTC TATCAATACAATGAACGAACTATTTATTCCTATTTTTTGTTATCTTAATGAGACAAAGACAAGC CTTCTTTACCACTTTAGGGGAAGTAAAGCTAAAAGAAAAATGTGATAGTAACAATAACATTGAT AGTGGTTAAATTGTATCAAAATCAAAACCTGAGATGCAATACCAAAAATGAGAATAAGTACCCT GCAATAGCTTTAATCAAAGAACAGTTCCAATTGAGTAATGGAATCTAGCCTATGGAATTTCTCC GTCAAAAAAGGGGTGAAAATTATACCACAATCTAGACTAGACTAAAAGTGAACTGGGAGAAAGG AACAAATTCAACATAGTGAAACAATGGCCATGAAAGATGATAGATAATATTACCAGCAGCTGGC TTGAGCATATTCTTCGAAACATCCCCATAAAGTCCTCCTGGAAGGTCGCTGGCC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_017826 						
					
						PMCS
						0.35814932

Conservation Information
Conserved circRNAs	osi_circ_017826
PMCS	0.35814932

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							15449644-15449080(-)
15447752-15449190(-)
						
						Potential amino acid sequence
						
							MLKPAAAVSVEQAEASAHLPKGDMWSVHKFGGTCMGTSQRIQNVADIILRDPSERKLVVVSAMS
KVTDMMYNLVNKAQSRDDSYITALDEVFEKHMAAAKDLLGGEDLARFLSQLHADVSNLKAMLRA
ICIAGHATESFSDFVVGHGEIWSAQLLSFAIKKSGTPCSWMDTREVLVVNPTGSNQVDPDYLES
EKRLEKWFARQPAETIIATGFIASTPENIPTTLKRDGSDFSAAIIGSLVKAGQVTIWTDVDGVF
SADPRKGPATFQEDFMGMFRRICSSQLLLFQLSKLKLVLTFQKVTCGLCINLVGPAWEPPRGFR
MWLT*(-)
MLMGSLVQIPEKGQRPSRRTLWGCFEEYAQASCCCFS*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	15449644-15449080(-) 15447752-15449190(-)
Potential amino acid sequence	MLKPAAAVSVEQAEASAHLPKGDMWSVHKFGGTCMGTSQRIQNVADIILRDPSERKLVVVSAMS KVTDMMYNLVNKAQSRDDSYITALDEVFEKHMAAAKDLLGGEDLARFLSQLHADVSNLKAMLRA ICIAGHATESFSDFVVGHGEIWSAQLLSFAIKKSGTPCSWMDTREVLVVNPTGSNQVDPDYLES EKRLEKWFARQPAETIIATGFIASTPENIPTTLKRDGSDFSAAIIGSLVKAGQVTIWTDVDGVF SADPRKGPATFQEDFMGMFRRICSSQLLLFQLSKLKLVLTFQKVTCGLCINLVGPAWEPPRGFR MWLT(-) MLMGSLVQIPEKGQRPSRRTLWGCFEEYAQASCCCFS(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015;Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os08g0342400_circ_g.5

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