Plant Circular RNA Database

Detail information of Os02g0674800_circ_g.8

			
						 General Information
					
						CircRNA Name
						Os02g0674800_circ_g.8
					
						ID in PlantcircBase
						osa_circ_015960
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 27493777-27494251  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder
					
						Parent gene
						
							Os02g0674800						
					
						Parent gene annotation
						
							Similar to OCL1 homeobox protein. (Os02t0674800-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os02g0674800_circ_g.6 Os02g0674800_circ_g.7 						
					
						Support reads
						2
					
						Tissues
						shoot, seed, pistil
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0674800-01:2

General Information
CircRNA Name	Os02g0674800_circ_g.8
ID in PlantcircBase	osa_circ_015960
Alias	NA
Organism	Oryza sativa
Position	chr2: 27493777-27494251 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, PcircRNA_finder
Parent gene	Os02g0674800
Parent gene annotation	Similar to OCL1 homeobox protein. (Os02t0674800-01)
Parent gene strand	-
Alternative splicing	Os02g0674800_circ_g.6 Os02g0674800_circ_g.7
Support reads	2
Tissues	shoot, seed, pistil
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0674800-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TCTCCGCCTCAGTGTCCCTCGTCATCGCGCCGCCCGCGCTAGAACCAGCGCCAGCGCCGCCGCC
GAGCATCCGCCCGCCGATGCCACCGCCGGCGTTGTCcttcatctgcgtgcggcgattctggaac
cagaacttgacctgccgcgcatcgagggagagcctcctgctgagctcggcgcgctgcttctcgt
ccggatgg

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 363
Transcript exons: 27493777-27493894,27494007-27494251
CTTCATCTGCGTGCGGCGATTCTGGAACCAGAACTTGACCTGCCGCGCATCGAGGGAGAGCCTC
CTGCTGAGCTCGGCGCGCTGCTTCTCGTCCGGATGGGGGCACTCCTTGAACAGCGCTTCGAGCT
CTTGGATCTGCTGCGGCGTGTGGCGGTGGTATCGCTTCTTCCTCTTGCGGGAGTTGCTGGGCTC
GGCGTCCTCGACGTCGTCCTCGCCGGCGGCCGAGATGGCGTCGAGATGGTCGCTTCCCGACCGG
CTGTCGTTCTCCGCCTCAGTGTCCCTCGTCATCGCGCCGCCCGCGCTAGAACCAGCGCCAGCGC
CGCCGCCGAGCATCCGCCCGCCGATGCCACCGCCGGCGTTGTC
						
						Genomic sequence
						
							CTTCATCTGCGTGCGGCGATTCTGGAACCAGAACTTGACCTGCCGCGCATCGAGGGAGAGCCTC
CTGCTGAGCTCGGCGCGCTGCTTCTCGTCCGGATGGGGGCACTCCTTGAACAGCCTACACAAGA
CGGACGCATCGATCCATGCATGAGAGGACAAAGAGAAAAAGGTTGGCACAGAGGAGAAGGATGC
AAAGTACGAGCGAGCGTTGAGAGGGGAATTTGCTTTACGCTTCGAGCTCTTGGATCTGCTGCGG
CGTGTGGCGGTGGTATCGCTTCTTCCTCTTGCGGGAGTTGCTGGGCTCGGCGTCCTCGACGTCG
TCCTCGCCGGCGGCCGAGATGGCGTCGAGATGGTCGCTTCCCGACCGGCTGTCGTTCTCCGCCT
CAGTGTCCCTCGTCATCGCGCCGCCCGCGCTAGAACCAGCGCCAGCGCCGCCGCCGAGCATCCG
CCCGCCGATGCCACCGCCGGCGTTGTC

Sequences
Splice junction sequence	TCTCCGCCTCAGTGTCCCTCGTCATCGCGCCGCCCGCGCTAGAACCAGCGCCAGCGCCGCCGCC GAGCATCCGCCCGCCGATGCCACCGCCGGCGTTGTCcttcatctgcgtgcggcgattctggaac cagaacttgacctgccgcgcatcgagggagagcctcctgctgagctcggcgcgctgcttctcgt ccggatgg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 363 Transcript exons: 27493777-27493894,27494007-27494251 CTTCATCTGCGTGCGGCGATTCTGGAACCAGAACTTGACCTGCCGCGCATCGAGGGAGAGCCTC CTGCTGAGCTCGGCGCGCTGCTTCTCGTCCGGATGGGGGCACTCCTTGAACAGCGCTTCGAGCT CTTGGATCTGCTGCGGCGTGTGGCGGTGGTATCGCTTCTTCCTCTTGCGGGAGTTGCTGGGCTC GGCGTCCTCGACGTCGTCCTCGCCGGCGGCCGAGATGGCGTCGAGATGGTCGCTTCCCGACCGG CTGTCGTTCTCCGCCTCAGTGTCCCTCGTCATCGCGCCGCCCGCGCTAGAACCAGCGCCAGCGC CGCCGCCGAGCATCCGCCCGCCGATGCCACCGCCGGCGTTGTC
Genomic sequence	CTTCATCTGCGTGCGGCGATTCTGGAACCAGAACTTGACCTGCCGCGCATCGAGGGAGAGCCTC CTGCTGAGCTCGGCGCGCTGCTTCTCGTCCGGATGGGGGCACTCCTTGAACAGCCTACACAAGA CGGACGCATCGATCCATGCATGAGAGGACAAAGAGAAAAAGGTTGGCACAGAGGAGAAGGATGC AAAGTACGAGCGAGCGTTGAGAGGGGAATTTGCTTTACGCTTCGAGCTCTTGGATCTGCTGCGG CGTGTGGCGGTGGTATCGCTTCTTCCTCTTGCGGGAGTTGCTGGGCTCGGCGTCCTCGACGTCG TCCTCGCCGGCGGCCGAGATGGCGTCGAGATGGTCGCTTCCCGACCGGCTGTCGTTCTCCGCCT CAGTGTCCCTCGTCATCGCGCCGCCCGCGCTAGAACCAGCGCCAGCGCCGCCGCCGAGCATCCG CCCGCCGATGCCACCGCCGGCGTTGTC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_004172* osi_circ_012108 						
					
						PMCS
						0.379509526

Conservation Information
Conserved circRNAs	osi_circ_004172* osi_circ_012108
PMCS	0.379509526

			
				
						 Functional Information
					

				
						Coding potential
						Y
					

						Potential coding position
						
							27493874-27494248(+)
27494116-27494248(+)
27494223-27493779(-)
						
					

						Potential amino acid sequence
						
							MGALLEQRFELLDLLRRVAVVSLLPLAGVAGLGVLDVVLAGGRDGVEMVASRPAVVLRLSVPRH
RAARARTSASAAAEHPPADATAGVVLHLRAAILEPELDLPRIEGEPPAELGALLLVRMGALLEQ
RFELLDLLRRVAVVSLLPLAGVAGLGVLDVVLAGGRDGVEMVASRPAVVLRLSVPRHRAARART
SASAAAEHPPADATAGVVLHLRAAILEPELDLPRIEGEPPAELGALLLVRMGALLEQRFELLDL
LRRVAVVSLLPLAGVAGLGVLDVVLAGGRDGVEMVASRPAVVLRLSVPRHRAARARTSASAAAE
HPPADATAGVVLHLRAAILEPELDLPRIEGEPPAELGALLLVRMGALLEQRFELLDLLRRVAVV
SLLPLAGVAGLGVLDVVLAGGRDGVEMVASRPAVVLRLSVPRHRAARARTSASAAAEHPPADAT
AGVV(+)
MASRWSLPDRLSFSASVSLVIAPPALEPAPAPPPSIRPPMPPPALSFICVRRFWNQNLTCRASR
ESLLLSSARCFSSGWGHSLNSASSSWICCGVWRWYRFFLLRELLGSASSTSSSPAAEMASRWSL
PDRLSFSASVSLVIAPPALEPAPAPPPSIRPPMPPPALSFICVRRFWNQNLTCRASRESLLLSS
ARCFSSGWGHSLNSASSSWICCGVWRWYRFFLLRELLGSASSTSSSPAAEMASRWSLPDRLSFS
ASVSLVIAPPALEPAPAPPPSIRPPMPPPALSFICVRRFWNQNLTCRASRESLLLSSARCFSSG
WGHSLNSASSSWICCGVWRWYRFFLLRELLGSASSTSSSPAAEMASRWSLPDRLSFSASVSLVI
APPALEPAPAPPPSIRPPMPPPAL(+)
MLGGGAGAGSSAGGAMTRDTEAENDSRSGSDHLDAISAAGEDDVEDAEPSNSRKRKKRYHRHTP
QQIQELEALFKECPHPDEKQRAELSRRLSLDARQVKFWFQNRRTQMKDNAGGGIGGRMLGGGAG
AGSSAGGAMTRDTEAENDSRSGSDHLDAISAAGEDDVEDAEPSNSRKRKKRYHRHTPQQIQELE
ALFKECPHPDEKQRAELSRRLSLDARQVKFWFQNRRTQMKDNAGGGIGGRMLGGGAGAGSSAGG
AMTRDTEAENDSRSGSDHLDAISAAGEDDVEDAEPSNSRKRKKRYHRHTPQQIQELEALFKECP
HPDEKQRAELSRRLSLDARQVKFWFQNRRTQMKDNAGGGIGGRMLGGGAGAGSSAGGAMTRDTE
AENDSRSGSDHLDAISAAGEDDVEDAEPSNSRKRKKRYHRHTPQQIQELEALFKECPHPDEKQR
AELSRRLSLDARQVKFWFQNRRTQMK(-)
						
					

						Sponge-miRNAs
						
							NA						
					

						circRNA-miRNA-mRNA network
						 VISUALIZATION
					

						Potential function description
						NA
					

			
		

Functional Information
Coding potential	Y
Potential coding position	27493874-27494248(+) 27494116-27494248(+) 27494223-27493779(-)
Potential amino acid sequence	MGALLEQRFELLDLLRRVAVVSLLPLAGVAGLGVLDVVLAGGRDGVEMVASRPAVVLRLSVPRH RAARARTSASAAAEHPPADATAGVVLHLRAAILEPELDLPRIEGEPPAELGALLLVRMGALLEQ RFELLDLLRRVAVVSLLPLAGVAGLGVLDVVLAGGRDGVEMVASRPAVVLRLSVPRHRAARART SASAAAEHPPADATAGVVLHLRAAILEPELDLPRIEGEPPAELGALLLVRMGALLEQRFELLDL LRRVAVVSLLPLAGVAGLGVLDVVLAGGRDGVEMVASRPAVVLRLSVPRHRAARARTSASAAAE HPPADATAGVVLHLRAAILEPELDLPRIEGEPPAELGALLLVRMGALLEQRFELLDLLRRVAVV SLLPLAGVAGLGVLDVVLAGGRDGVEMVASRPAVVLRLSVPRHRAARARTSASAAAEHPPADAT AGVV(+) MASRWSLPDRLSFSASVSLVIAPPALEPAPAPPPSIRPPMPPPALSFICVRRFWNQNLTCRASR ESLLLSSARCFSSGWGHSLNSASSSWICCGVWRWYRFFLLRELLGSASSTSSSPAAEMASRWSL PDRLSFSASVSLVIAPPALEPAPAPPPSIRPPMPPPALSFICVRRFWNQNLTCRASRESLLLSS ARCFSSGWGHSLNSASSSWICCGVWRWYRFFLLRELLGSASSTSSSPAAEMASRWSLPDRLSFS ASVSLVIAPPALEPAPAPPPSIRPPMPPPALSFICVRRFWNQNLTCRASRESLLLSSARCFSSG WGHSLNSASSSWICCGVWRWYRFFLLRELLGSASSTSSSPAAEMASRWSLPDRLSFSASVSLVI APPALEPAPAPPPSIRPPMPPPAL(+) MLGGGAGAGSSAGGAMTRDTEAENDSRSGSDHLDAISAAGEDDVEDAEPSNSRKRKKRYHRHTP QQIQELEALFKECPHPDEKQRAELSRRLSLDARQVKFWFQNRRTQMKDNAGGGIGGRMLGGGAG AGSSAGGAMTRDTEAENDSRSGSDHLDAISAAGEDDVEDAEPSNSRKRKKRYHRHTPQQIQELE ALFKECPHPDEKQRAELSRRLSLDARQVKFWFQNRRTQMKDNAGGGIGGRMLGGGAGAGSSAGG AMTRDTEAENDSRSGSDHLDAISAAGEDDVEDAEPSNSRKRKKRYHRHTPQQIQELEALFKECP HPDEKQRAELSRRLSLDARQVKFWFQNRRTQMKDNAGGGIGGRMLGGGAGAGSSAGGAMTRDTE AENDSRSGSDHLDAISAAGEDDVEDAEPSNSRKRKKRYHRHTPQQIQELEALFKECPHPDEKQR AELSRRLSLDARQVKFWFQNRRTQMK(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os02g0674800_circ_g.8

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