Plant Circular RNA Database

Detail information of AT5G01920_circ_g.2

			
						 General Information
					
						CircRNA Name
						AT5G01920_circ_g.2
					
						ID in PlantcircBase
						ath_circ_035946
					
						Alias
						Chr5:359783|360446
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 359783-360446  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							ei-circRNA							
									
						Identification method
						CIRCexplorer; CIRI
					
						Parent gene
						
							AT5G01920						
					
						Parent gene annotation
						
							Serine/threonine-protein kinase STN8, chloroplastic
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT5G01920_circ_g.3 AT5G01920_circ_g.4 AT5G01920_circ_g.5 						
					
						Support reads
						4
					
						Tissues
						seedling
					
						Exon boundary  
						
							No-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-CA							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G01920.1:2
AT5G01920.2:2

General Information
CircRNA Name	AT5G01920_circ_g.2
ID in PlantcircBase	ath_circ_035946
Alias	Chr5:359783\|360446
Organism	Arabidpsis thaliana
Position	chr5: 359783-360446 JBrowse»
Reference genome	TAIR10.38
Type	ei-circRNA
Identification method	CIRCexplorer; CIRI
Parent gene	AT5G01920
Parent gene annotation	Serine/threonine-protein kinase STN8, chloroplastic
Parent gene strand	+
Alternative splicing	AT5G01920_circ_g.3 AT5G01920_circ_g.4 AT5G01920_circ_g.5
Support reads	4
Tissues	seedling
Exon boundary	No-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-CA The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G01920.1:2 AT5G01920.2:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							YES							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							Convergent:(F):AAGGAGTCGAGTCTGTGAAACG
(R):GTAAAGTGCGTTCCGGGATGTA.Divergent:(F):TACATCCCGGAACGCACTTTAC
(R)CGAACCTCCAGACAAGCCATTT
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	YES Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	Convergent:(F):AAGGAGTCGAGTCTGTGAAACG (R):GTAAAGTGCGTTCCGGGATGTA.Divergent:(F):TACATCCCGGAACGCACTTTAC (R)CGAACCTCCAGACAAGCCATTT PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TCCTGACTACTGTCCTCCTGAGCTCTATGTCCTCCCGGAGGAAACGCCAAGCCCTCCTCCGGAG
CCTATAGCCGCTTTGCTTTCCCCGATTCTCTGGCAGggtcaaggtgggagtacgaggggccgag
gaatttggcgagtacgaagagtggttcaactaccgactctcaagggccgctcctgacacctgcg
ctgagttt

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GGTCAAGGTGGGAGTACGAGGGGCCGAGGAATTTGGCGAGTACGAAGAGTGGTTCAACTACCGA
CTCTCAAGGGCCGCTCCTGACACCTGCGCTGAGTTTCTCGGAAGCTTTGTCGCCGACAAGACCA
ATACTATGTTTACTAAAGGCGGCAAATGGCTTGTCTGGAGGTTCGAGGTGTGGTCTTCTTACTT
ACCCTTACAATGAATCTGCTGTTTATAGTTGCGATGATGATGATGAATGAAATGTTTTCTTCAA
CAGGGAGACCGTGATCTCGCTGATTACATGAAAGATCGGAGCTTTCCTTCTAATTTGGAGTCCA
TCATGTTTGGGCGCGTTCTCCAAGGAGTCGAGTCTGTGAAACGTCGTGCACTGATCATTAAGCA
GATTATGCGCCAGATCATTACATCTCTCAGGAAAATCCATGGCACGGGCATTGTTCACCGGGAC
GTGAAGCCTGCCAACTTGGTGGTTACAAAGAAGGGCCAGATTAAGCTCATCGACTTTGGTGCCG
CTGCTGATCTTCGCATTGGCAAGAATTACATCCCGGAACGCACTTTACTCGATCCTGACTACTG
TCCTCCTGAGCTCTATGTCCTCCCGGAGGAAACGCCAAGCCCTCCTCCGGAGCCTATAGCCGCT
TTGCTTTCCCCGATTCTCTGGCAG

Sequences
Splice junction sequence	TCCTGACTACTGTCCTCCTGAGCTCTATGTCCTCCCGGAGGAAACGCCAAGCCCTCCTCCGGAG CCTATAGCCGCTTTGCTTTCCCCGATTCTCTGGCAGggtcaaggtgggagtacgaggggccgag gaatttggcgagtacgaagagtggttcaactaccgactctcaagggccgctcctgacacctgcg ctgagttt
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GGTCAAGGTGGGAGTACGAGGGGCCGAGGAATTTGGCGAGTACGAAGAGTGGTTCAACTACCGA CTCTCAAGGGCCGCTCCTGACACCTGCGCTGAGTTTCTCGGAAGCTTTGTCGCCGACAAGACCA ATACTATGTTTACTAAAGGCGGCAAATGGCTTGTCTGGAGGTTCGAGGTGTGGTCTTCTTACTT ACCCTTACAATGAATCTGCTGTTTATAGTTGCGATGATGATGATGAATGAAATGTTTTCTTCAA CAGGGAGACCGTGATCTCGCTGATTACATGAAAGATCGGAGCTTTCCTTCTAATTTGGAGTCCA TCATGTTTGGGCGCGTTCTCCAAGGAGTCGAGTCTGTGAAACGTCGTGCACTGATCATTAAGCA GATTATGCGCCAGATCATTACATCTCTCAGGAAAATCCATGGCACGGGCATTGTTCACCGGGAC GTGAAGCCTGCCAACTTGGTGGTTACAAAGAAGGGCCAGATTAAGCTCATCGACTTTGGTGCCG CTGCTGATCTTCGCATTGGCAAGAATTACATCCCGGAACGCACTTTACTCGATCCTGACTACTG TCCTCCTGAGCTCTATGTCCTCCCGGAGGAAACGCCAAGCCCTCCTCCGGAGCCTATAGCCGCT TTGCTTTCCCCGATTCTCTGGCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.235375891

Conservation Information
Conserved circRNAs	NA
PMCS	0.235375891

			
						 Functional Information
					
						Coding potential
						NA
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							th-miR861-5p NA 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to methyl jasmonate (validated)

Functional Information
Coding potential	NA
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	th-miR861-5p NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to methyl jasmonate (validated)

			
						 Other Information
					
						References
						Zhang et al., 2020

circRNA

Detail information of AT5G01920_circ_g.2

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