Plant Circular RNA Database

Detail information of AT4G02260_circ_g.5

			
						 General Information
					
						CircRNA Name
						AT4G02260_circ_g.5
					
						ID in PlantcircBase
						ath_circ_029351
					
						Alias
						At_ciR5263
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr4: 987976-989309  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							AT4G02260						
					
						Parent gene annotation
						
							RSH1
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT4G02260_circ_g.3 AT4G02260_circ_g.4 AT4G02260_circ_g.6 AT4G02260_circ_g.7 AT4G02260_circ_g.8 AT4G02260_circ_g.9 						
					
						Support reads
						2
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT4G02260.4:7
AT4G02260.1:7
AT4G02260.3:7
AT4G02260.2:7

General Information
CircRNA Name	AT4G02260_circ_g.5
ID in PlantcircBase	ath_circ_029351
Alias	At_ciR5263
Organism	Arabidpsis thaliana
Position	chr4: 987976-989309 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	find_circ
Parent gene	AT4G02260
Parent gene annotation	RSH1
Parent gene strand	+
Alternative splicing	AT4G02260_circ_g.3 AT4G02260_circ_g.4 AT4G02260_circ_g.6 AT4G02260_circ_g.7 AT4G02260_circ_g.8 AT4G02260_circ_g.9
Support reads	2
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT4G02260.4:7 AT4G02260.1:7 AT4G02260.3:7 AT4G02260.2:7

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CAAGTCTCTATCTACTGGATTAGTTGGAAACGCGGTTCCTTTAGGTAGAAATTCAAGGGGGAAG
ACGGGTTGCCTCAACAATGCAGATTTTGCACTCAGGtctgaactggaaaatctgtctttcatgt
acgtaagtgctgaggattatgatagagtcactagcaggattgctaacctctacaaagagcatga
aaaagaac

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 648
Transcript exons: 987976-988083,988263-988360,988443-988503,988619-988681,
988771-988827,988897-988998,989151-989309
TCTGAACTGGAAAATCTGTCTTTCATGTACGTAAGTGCTGAGGATTATGATAGAGTCACTAGCA
GGATTGCTAACCTCTACAAAGAGCATGAAAAAGAACTCACTGAGGCAAACAGAATTTTGGTGAA
AAAGATTGAAGATGATCAGTTTCTGGACCTTGTGACTGTGAATACTGATGTTCGATCTGTTTGC
AAGGAAACTTACAGCATCTACAAAGCTGCTCTCAAATCGAAAGGATCAATTAATGATTACAACC
AGATTGCTCAGTTACGGATTGTTGTAAAGCCAAAACCATCTGTAGGGGTCGGGCCTTTGTGCAG
TCCACAACAGATATGCTATCACGTTCTGGGGCTTGTTCATGAGATCTGGAAACCTATTCCACGA
ACAGTAAAAGATTACATTGCAACCCCGAAGCCCAATGGATACCAGAGCCTCCATACTACTGTGA
TTCCATTCTTGTATGAGAGTATGTTTCGACTGGAGGTTCAGATCAGAACCGAGGAGATGGACTT
GATTGCTGAAAGGGGCATTGCTGTTTACTACAATGGCAAGTCTCTATCTACTGGATTAGTTGGA
AACGCGGTTCCTTTAGGTAGAAATTCAAGGGGGAAGACGGGTTGCCTCAACAATGCAGATTTTG
CACTCAGG
						
					

						Genomic sequence
						
							TCTGAACTGGAAAATCTGTCTTTCATGTACGTAAGTGCTGAGGATTATGATAGAGTCACTAGCA
GGATTGCTAACCTCTACAAAGAGCATGAAAAAGAACTCACTGAGGTAATTTCAACTATTATCGT
CTCTGAGCTTAAAATGCCAAAACATAGATTGAAAAACAAAAGGATATTAGAGAAGAATGTTGAT
TAGCGACCTACACCTCTTTGACCTTTCGAACTCTGAATTCATCTGGGCGTTTATTTAGTTCATC
TGCATTCACAGAGTCCTACTATGTATCTTAGGCAAACAGAATTTTGGTGAAAAAGATTGAAGAT
GATCAGTTTCTGGACCTTGTGACTGTGAATACTGATGTTCGATCTGTTTGCAAGGAAACTTACA
GGTGAATTCATAAGTGACTTCTTCATTTTACCTTGTAACCTACCCTTTTCTTCAGCATCTGAAG
TCTCTTTTTTTATCTGCAGCATCTACAAAGCTGCTCTCAAATCGAAAGGATCAATTAATGATTA
CAACCAGATTGCTCAGGTTTGTGTTATACTCTTGTCTCCAAGTTGTATCAAAGGTTTTGAATAC
AAAACTTCCATCTGAAGCTTATATGATTGTTAACACGCTGCCTCTCCTAATTTCATTATTTTAG
CAGTTACGGATTGTTGTAAAGCCAAAACCATCTGTAGGGGTCGGGCCTTTGTGCAGTCCACAAC
AGGTGAAATCCCAATGAAAAGGAAATGGGTATATTTTGTTCCGATTCGTTCTTGTGTGTCACTT
AGCTTTCTCGCTCTGTCGTCAATGCAGATATGCTATCACGTTCTGGGGCTTGTTCATGAGATCT
GGAAACCTATTCCACGAACAGTATGTATCAGATCTTTTGTTATTCAGGTTATATCTTATCTACA
TTGGGACTGAACATATGTCTTACAGGTAAAAGATTACATTGCAACCCCGAAGCCCAATGGATAC
CAGAGCCTCCATACTACTGTGATTCCATTCTTGTATGAGAGTATGTTTCGACTGGAGGTTCAGG
TATTTGAGATAAAAATCTTTTATCACAGTTCTTAGAAAAACGCCGATGATTAGTACAATTGAAT
TCGGTTTTAGTATTTTAAAGGTTCAAGCATGGGGAATATTTAAAAGGCTGATTTGATTGGTTAT
TAAGACTACATTTTTCTCTGCAGATCAGAACCGAGGAGATGGACTTGATTGCTGAAAGGGGCAT
TGCTGTTTACTACAATGGCAAGTCTCTATCTACTGGATTAGTTGGAAACGCGGTTCCTTTAGGT
AGAAATTCAAGGGGGAAGACGGGTTGCCTCAACAATGCAGATTTTGCACTCAGG
						
					

			
		

Sequences
Splice junction sequence	CAAGTCTCTATCTACTGGATTAGTTGGAAACGCGGTTCCTTTAGGTAGAAATTCAAGGGGGAAG ACGGGTTGCCTCAACAATGCAGATTTTGCACTCAGGtctgaactggaaaatctgtctttcatgt acgtaagtgctgaggattatgatagagtcactagcaggattgctaacctctacaaagagcatga aaaagaac
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 648 Transcript exons: 987976-988083,988263-988360,988443-988503,988619-988681, 988771-988827,988897-988998,989151-989309 TCTGAACTGGAAAATCTGTCTTTCATGTACGTAAGTGCTGAGGATTATGATAGAGTCACTAGCA GGATTGCTAACCTCTACAAAGAGCATGAAAAAGAACTCACTGAGGCAAACAGAATTTTGGTGAA AAAGATTGAAGATGATCAGTTTCTGGACCTTGTGACTGTGAATACTGATGTTCGATCTGTTTGC AAGGAAACTTACAGCATCTACAAAGCTGCTCTCAAATCGAAAGGATCAATTAATGATTACAACC AGATTGCTCAGTTACGGATTGTTGTAAAGCCAAAACCATCTGTAGGGGTCGGGCCTTTGTGCAG TCCACAACAGATATGCTATCACGTTCTGGGGCTTGTTCATGAGATCTGGAAACCTATTCCACGA ACAGTAAAAGATTACATTGCAACCCCGAAGCCCAATGGATACCAGAGCCTCCATACTACTGTGA TTCCATTCTTGTATGAGAGTATGTTTCGACTGGAGGTTCAGATCAGAACCGAGGAGATGGACTT GATTGCTGAAAGGGGCATTGCTGTTTACTACAATGGCAAGTCTCTATCTACTGGATTAGTTGGA AACGCGGTTCCTTTAGGTAGAAATTCAAGGGGGAAGACGGGTTGCCTCAACAATGCAGATTTTG CACTCAGG
Genomic sequence	TCTGAACTGGAAAATCTGTCTTTCATGTACGTAAGTGCTGAGGATTATGATAGAGTCACTAGCA GGATTGCTAACCTCTACAAAGAGCATGAAAAAGAACTCACTGAGGTAATTTCAACTATTATCGT CTCTGAGCTTAAAATGCCAAAACATAGATTGAAAAACAAAAGGATATTAGAGAAGAATGTTGAT TAGCGACCTACACCTCTTTGACCTTTCGAACTCTGAATTCATCTGGGCGTTTATTTAGTTCATC TGCATTCACAGAGTCCTACTATGTATCTTAGGCAAACAGAATTTTGGTGAAAAAGATTGAAGAT GATCAGTTTCTGGACCTTGTGACTGTGAATACTGATGTTCGATCTGTTTGCAAGGAAACTTACA GGTGAATTCATAAGTGACTTCTTCATTTTACCTTGTAACCTACCCTTTTCTTCAGCATCTGAAG TCTCTTTTTTTATCTGCAGCATCTACAAAGCTGCTCTCAAATCGAAAGGATCAATTAATGATTA CAACCAGATTGCTCAGGTTTGTGTTATACTCTTGTCTCCAAGTTGTATCAAAGGTTTTGAATAC AAAACTTCCATCTGAAGCTTATATGATTGTTAACACGCTGCCTCTCCTAATTTCATTATTTTAG CAGTTACGGATTGTTGTAAAGCCAAAACCATCTGTAGGGGTCGGGCCTTTGTGCAGTCCACAAC AGGTGAAATCCCAATGAAAAGGAAATGGGTATATTTTGTTCCGATTCGTTCTTGTGTGTCACTT AGCTTTCTCGCTCTGTCGTCAATGCAGATATGCTATCACGTTCTGGGGCTTGTTCATGAGATCT GGAAACCTATTCCACGAACAGTATGTATCAGATCTTTTGTTATTCAGGTTATATCTTATCTACA TTGGGACTGAACATATGTCTTACAGGTAAAAGATTACATTGCAACCCCGAAGCCCAATGGATAC CAGAGCCTCCATACTACTGTGATTCCATTCTTGTATGAGAGTATGTTTCGACTGGAGGTTCAGG TATTTGAGATAAAAATCTTTTATCACAGTTCTTAGAAAAACGCCGATGATTAGTACAATTGAAT TCGGTTTTAGTATTTTAAAGGTTCAAGCATGGGGAATATTTAAAAGGCTGATTTGATTGGTTAT TAAGACTACATTTTTCTCTGCAGATCAGAACCGAGGAGATGGACTTGATTGCTGAAAGGGGCAT TGCTGTTTACTACAATGGCAAGTCTCTATCTACTGGATTAGTTGGAAACGCGGTTCCTTTAGGT AGAAATTCAAGGGGGAAGACGGGTTGCCTCAACAATGCAGATTTTGCACTCAGG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.234285094

Conservation Information
Conserved circRNAs	NA
PMCS	0.234285094

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							988000-989306(+)
						
						Potential amino acid sequence
						
							MYVSAEDYDRVTSRIANLYKEHEKELTEANRILVKKIEDDQFLDLVTVNTDVRSVCKETYSIYK
AALKSKGSINDYNQIAQLRIVVKPKPSVGVGPLCSPQQICYHVLGLVHEIWKPIPRTVKDYIAT
PKPNGYQSLHTTVIPFLYESMFRLEVQIRTEEMDLIAERGIAVYYNGKSLSTGLVGNAVPLGRN
SRGKTGCLNNADFALRSELENLSFMYVSAEDYDRVTSRIANLYKEHEKELTEANRILVKKIEDD
QFLDLVTVNTDVRSVCKETYSIYKAALKSKGSINDYNQIAQLRIVVKPKPSVGVGPLCSPQQIC
YHVLGLVHEIWKPIPRTVKDYIATPKPNGYQSLHTTVIPFLYESMFRLEVQIRTEEMDLIAERG
IAVYYNGKSLSTGLVGNAVPLGRNSRGKTGCLNNADFALRSELENLSFMYVSAEDYDRVTSRIA
NLYKEHEKELTEANRILVKKIEDDQFLDLVTVNTDVRSVCKETYSIYKAALKSKGSINDYNQIA
QLRIVVKPKPSVGVGPLCSPQQICYHVLGLVHEIWKPIPRTVKDYIATPKPNGYQSLHTTVIPF
LYESMFRLEVQIRTEEMDLIAERGIAVYYNGKSLSTGLVGNAVPLGRNSRGKTGCLNNADFALR
SELENLSFMYVSAEDYDRVTSRIANLYKEHEKELTEANRILVKKIEDDQFLDLVTVNTDVRSVC
KETYSIYKAALKSKGSINDYNQIAQLRIVVKPKPSVGVGPLCSPQQICYHVLGLVHEIWKPIPR
TVKDYIATPKPNGYQSLHTTVIPFLYESMFRLEVQIRTEEMDLIAERGIAVYYNGKSLSTGLVG
NAVPLGRNSRGKTGCLNNADFALR(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	988000-989306(+)
Potential amino acid sequence	MYVSAEDYDRVTSRIANLYKEHEKELTEANRILVKKIEDDQFLDLVTVNTDVRSVCKETYSIYK AALKSKGSINDYNQIAQLRIVVKPKPSVGVGPLCSPQQICYHVLGLVHEIWKPIPRTVKDYIAT PKPNGYQSLHTTVIPFLYESMFRLEVQIRTEEMDLIAERGIAVYYNGKSLSTGLVGNAVPLGRN SRGKTGCLNNADFALRSELENLSFMYVSAEDYDRVTSRIANLYKEHEKELTEANRILVKKIEDD QFLDLVTVNTDVRSVCKETYSIYKAALKSKGSINDYNQIAQLRIVVKPKPSVGVGPLCSPQQIC YHVLGLVHEIWKPIPRTVKDYIATPKPNGYQSLHTTVIPFLYESMFRLEVQIRTEEMDLIAERG IAVYYNGKSLSTGLVGNAVPLGRNSRGKTGCLNNADFALRSELENLSFMYVSAEDYDRVTSRIA NLYKEHEKELTEANRILVKKIEDDQFLDLVTVNTDVRSVCKETYSIYKAALKSKGSINDYNQIA QLRIVVKPKPSVGVGPLCSPQQICYHVLGLVHEIWKPIPRTVKDYIATPKPNGYQSLHTTVIPF LYESMFRLEVQIRTEEMDLIAERGIAVYYNGKSLSTGLVGNAVPLGRNSRGKTGCLNNADFALR SELENLSFMYVSAEDYDRVTSRIANLYKEHEKELTEANRILVKKIEDDQFLDLVTVNTDVRSVC KETYSIYKAALKSKGSINDYNQIAQLRIVVKPKPSVGVGPLCSPQQICYHVLGLVHEIWKPIPR TVKDYIATPKPNGYQSLHTTVIPFLYESMFRLEVQIRTEEMDLIAERGIAVYYNGKSLSTGLVG NAVPLGRNSRGKTGCLNNADFALR(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of AT4G02260_circ_g.5

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