Plant Circular RNA Database

Detail information of Solyc04g045470.2_circ_g.1

			
						 General Information
					
						CircRNA Name
						Solyc04g045470.2_circ_g.1
					
						ID in PlantcircBase
						sly_circ_001375
					
						Alias
						4:32672667|32686338
					
						Organism
						
							Solanum lycopersicum						
					
						Position
						
							chr4: 33524897-33538568  JBrowse»						
					
						Reference genome
						
							SL2.50.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Solyc04g045470.2						
					
						Parent gene annotation
						
							protein_coding
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Solyc04g045470.2_circ_g.2 Solyc04g045470.2_circ_g.3 Solyc04g045470.2_circ_g.4 						
					
						Support reads
						12
					
						Tissues
						fruit
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Solyc04g045470.2.1:6

General Information
CircRNA Name	Solyc04g045470.2_circ_g.1
ID in PlantcircBase	sly_circ_001375
Alias	4:32672667\|32686338
Organism	Solanum lycopersicum
Position	chr4: 33524897-33538568 JBrowse»
Reference genome	SL2.50.38
Type	e-circRNA
Identification method	CIRI
Parent gene	Solyc04g045470.2
Parent gene annotation	protein_coding
Parent gene strand	+
Alternative splicing	Solyc04g045470.2_circ_g.2 Solyc04g045470.2_circ_g.3 Solyc04g045470.2_circ_g.4
Support reads	12
Tissues	fruit
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Solyc04g045470.2.1:6

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CCGTGAAAGAAGTTGGAGAGGAAAATTCAGGCGAAGAGGAAGAGTTATAAGGCCTGGTGCAAAC
CGAGTCGATGGCAAGTGCCCGCTAACTCCACTTGAGttaacaaatgtgtggaatcttaagttgc
atcaagtttggaagccttgtattgcccatactgtttctcaagaaggttttcatttttttcagcc
agtagcaa

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 810
Transcript exons: 33524897-33524969,33527143-33527219,33533730-33533818,33
536561-33536758,33538067-33538271,33538401-33538568
TTAACAAATGTGTGGAATCTTAAGTTGCATCAAGTTTGGAAGCCTTGTATTGCCCATACTGTTT
CTCAAGAAGGTTTTGCAGAACTGCCAAAGTCGAATGGTTACCTCATAATTGAAGCAAATGGTGG
ATTGAACCAACAAAGGTTATCGATCTGTGATGCTGTAGCAGTTGCAGGACTACTAAATGCTACT
CTTGTTATTCCAATTTTTCATTTAAATAGCGTATGGAGAGACTCTAGCAAGTTCTGTGACATAT
TTGATGAGGACTTCTTCATATATGCTCTAAGGAATCATGTGAAGGTGGTACGACAACTCCCTGA
AGATATGCTACTGAAGTTTGACAATAATATAAGTAGAATTGTTAACTTGAGAGTAAAGGCATGG
TCCAGCCCTACCTATTATCTTCTAAAGGTCCTGCCAAAGCTGAAGGAGTTAGGGGCTGTGCGCA
TTGCACCGTTTTCTAACAGATTGGCTCATTCGGTTCCTCCAAACATCCAAGGGTTGCGATGTTT
GTGCAACTTTGAAGCACTTAGGTTTTCCGAACCCATACGGATGCTTGCAGCAAAGATGGTTGAT
CGAATGGTAAAAAAGAGCTCTAAAACTAGTGGAAGATATGTTTCGGTGCACATCCGCTTTGAAG
AGGACATGGTTGCATTTTCATGCTGTATTTATGATGGCGGGGAAGAAGAAAAACATGAGATGGA
TATTGCCCGTGAAAGAAGTTGGAGAGGAAAATTCAGGCGAAGAGGAAGAGTTATAAGGCCTGGT
GCAAACCGAGTCGATGGCAAGTGCCCGCTAACTCCACTTGAG
						
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	CCGTGAAAGAAGTTGGAGAGGAAAATTCAGGCGAAGAGGAAGAGTTATAAGGCCTGGTGCAAAC CGAGTCGATGGCAAGTGCCCGCTAACTCCACTTGAGttaacaaatgtgtggaatcttaagttgc atcaagtttggaagccttgtattgcccatactgtttctcaagaaggttttcatttttttcagcc agtagcaa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 810 Transcript exons: 33524897-33524969,33527143-33527219,33533730-33533818,33 536561-33536758,33538067-33538271,33538401-33538568 TTAACAAATGTGTGGAATCTTAAGTTGCATCAAGTTTGGAAGCCTTGTATTGCCCATACTGTTT CTCAAGAAGGTTTTGCAGAACTGCCAAAGTCGAATGGTTACCTCATAATTGAAGCAAATGGTGG ATTGAACCAACAAAGGTTATCGATCTGTGATGCTGTAGCAGTTGCAGGACTACTAAATGCTACT CTTGTTATTCCAATTTTTCATTTAAATAGCGTATGGAGAGACTCTAGCAAGTTCTGTGACATAT TTGATGAGGACTTCTTCATATATGCTCTAAGGAATCATGTGAAGGTGGTACGACAACTCCCTGA AGATATGCTACTGAAGTTTGACAATAATATAAGTAGAATTGTTAACTTGAGAGTAAAGGCATGG TCCAGCCCTACCTATTATCTTCTAAAGGTCCTGCCAAAGCTGAAGGAGTTAGGGGCTGTGCGCA TTGCACCGTTTTCTAACAGATTGGCTCATTCGGTTCCTCCAAACATCCAAGGGTTGCGATGTTT GTGCAACTTTGAAGCACTTAGGTTTTCCGAACCCATACGGATGCTTGCAGCAAAGATGGTTGAT CGAATGGTAAAAAAGAGCTCTAAAACTAGTGGAAGATATGTTTCGGTGCACATCCGCTTTGAAG AGGACATGGTTGCATTTTCATGCTGTATTTATGATGGCGGGGAAGAAGAAAAACATGAGATGGA TATTGCCCGTGAAAGAAGTTGGAGAGGAAAATTCAGGCGAAGAGGAAGAGTTATAAGGCCTGGT GCAAACCGAGTCGATGGCAAGTGCCCGCTAACTCCACTTGAG
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.480434794

Conservation Information
Conserved circRNAs	NA
PMCS	0.480434794

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							33536647-33538565(+)
						
						Potential amino acid sequence
						
							MLLKFDNNISRIVNLRVKAWSSPTYYLLKVLPKLKELGAVRIAPFSNRLAHSVPPNIQGLRCLC
NFEALRFSEPIRMLAAKMVDRMVKKSSKTSGRYVSVHIRFEEDMVAFSCCIYDGGEEEKHEMDI
ARERSWRGKFRRRGRVIRPGANRVDGKCPLTPLELTNVWNLKLHQVWKPCIAHTVSQEGFAELP
KSNGYLIIEANGGLNQQRLSICDAVAVAGLLNATLVIPIFHLNSVWRDSSKFCDIFDEDFFIYA
LRNHVKVVRQLPEDMLLKFDNNISRIVNLRVKAWSSPTYYLLKVLPKLKELGAVRIAPFSNRLA
HSVPPNIQGLRCLCNFEALRFSEPIRMLAAKMVDRMVKKSSKTSGRYVSVHIRFEEDMVAFSCC
IYDGGEEEKHEMDIARERSWRGKFRRRGRVIRPGANRVDGKCPLTPLELTNVWNLKLHQVWKPC
IAHTVSQEGFAELPKSNGYLIIEANGGLNQQRLSICDAVAVAGLLNATLVIPIFHLNSVWRDSS
KFCDIFDEDFFIYALRNHVKVVRQLPEDMLLKFDNNISRIVNLRVKAWSSPTYYLLKVLPKLKE
LGAVRIAPFSNRLAHSVPPNIQGLRCLCNFEALRFSEPIRMLAAKMVDRMVKKSSKTSGRYVSV
HIRFEEDMVAFSCCIYDGGEEEKHEMDIARERSWRGKFRRRGRVIRPGANRVDGKCPLTPLELT
NVWNLKLHQVWKPCIAHTVSQEGFAELPKSNGYLIIEANGGLNQQRLSICDAVAVAGLLNATLV
IPIFHLNSVWRDSSKFCDIFDEDFFIYALRNHVKVVRQLPEDMLLKFDNNISRIVNLRVKAWSS
PTYYLLKVLPKLKELGAVRIAPFSNRLAHSVPPNIQGLRCLCNFEALRFSEPIRMLAAKMVDRM
VKKSSKTSGRYVSVHIRFEEDMVAFSCCIYDGGEEEKHEMDIARERSWRGKFRRRGRVIRPGAN
RVDGKCPLTPLE(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						fruit ripening

Functional Information
Coding potential	Y
Potential coding position	33536647-33538565(+)
Potential amino acid sequence	MLLKFDNNISRIVNLRVKAWSSPTYYLLKVLPKLKELGAVRIAPFSNRLAHSVPPNIQGLRCLC NFEALRFSEPIRMLAAKMVDRMVKKSSKTSGRYVSVHIRFEEDMVAFSCCIYDGGEEEKHEMDI ARERSWRGKFRRRGRVIRPGANRVDGKCPLTPLELTNVWNLKLHQVWKPCIAHTVSQEGFAELP KSNGYLIIEANGGLNQQRLSICDAVAVAGLLNATLVIPIFHLNSVWRDSSKFCDIFDEDFFIYA LRNHVKVVRQLPEDMLLKFDNNISRIVNLRVKAWSSPTYYLLKVLPKLKELGAVRIAPFSNRLA HSVPPNIQGLRCLCNFEALRFSEPIRMLAAKMVDRMVKKSSKTSGRYVSVHIRFEEDMVAFSCC IYDGGEEEKHEMDIARERSWRGKFRRRGRVIRPGANRVDGKCPLTPLELTNVWNLKLHQVWKPC IAHTVSQEGFAELPKSNGYLIIEANGGLNQQRLSICDAVAVAGLLNATLVIPIFHLNSVWRDSS KFCDIFDEDFFIYALRNHVKVVRQLPEDMLLKFDNNISRIVNLRVKAWSSPTYYLLKVLPKLKE LGAVRIAPFSNRLAHSVPPNIQGLRCLCNFEALRFSEPIRMLAAKMVDRMVKKSSKTSGRYVSV HIRFEEDMVAFSCCIYDGGEEEKHEMDIARERSWRGKFRRRGRVIRPGANRVDGKCPLTPLELT NVWNLKLHQVWKPCIAHTVSQEGFAELPKSNGYLIIEANGGLNQQRLSICDAVAVAGLLNATLV IPIFHLNSVWRDSSKFCDIFDEDFFIYALRNHVKVVRQLPEDMLLKFDNNISRIVNLRVKAWSS PTYYLLKVLPKLKELGAVRIAPFSNRLAHSVPPNIQGLRCLCNFEALRFSEPIRMLAAKMVDRM VKKSSKTSGRYVSVHIRFEEDMVAFSCCIYDGGEEEKHEMDIARERSWRGKFRRRGRVIRPGAN RVDGKCPLTPLE(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	fruit ripening

			
						 Other Information
					
						References
						Yin et al., 2018

circRNA

Detail information of Solyc04g045470.2_circ_g.1

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