Plant Circular RNA Database

Detail information of Os08g0137100_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os08g0137100_circ_g.3
					
						ID in PlantcircBase
						osa_circ_035768
					
						Alias
						Os_ciR11459
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr8: 2081897-2082706  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os08g0137100						
					
						Parent gene annotation
						
							WD40 Polycomb protein of the PRC2 complex, Regulation of vegetat
ive and reproductive development (Os08t0137100-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os08g0137100_circ_ag.1 Os08g0137100_circ_ag.2 Os08g0137100_circ_g.4 Os08g0137100_circ_g.5 Os08g0137100_circ_g.6 Os08g0137200_circ_g.1 Os08g0137200_circ_g.2 Os08g0137200_circ_g.3 Os08g0137200_circ_g.4 						
					
						Support reads
						2/3
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os08t0137100-01:4

General Information
CircRNA Name	Os08g0137100_circ_g.3
ID in PlantcircBase	osa_circ_035768
Alias	Os_ciR11459
Organism	Oryza sativa
Position	chr8: 2081897-2082706 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os08g0137100
Parent gene annotation	WD40 Polycomb protein of the PRC2 complex, Regulation of vegetat ive and reproductive development (Os08t0137100-01)
Parent gene strand	+
Alternative splicing	Os08g0137100_circ_ag.1 Os08g0137100_circ_ag.2 Os08g0137100_circ_g.4 Os08g0137100_circ_g.5 Os08g0137100_circ_g.6 Os08g0137200_circ_g.1 Os08g0137200_circ_g.2 Os08g0137200_circ_g.3 Os08g0137200_circ_g.4
Support reads	2/3
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os08t0137100-01:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AAATCTTGTATTTCTGCATGCCAACACATTATTTTTACAGGCTGACTAATCCGCAATGCAAATC
TGCGATAAGGCAGACTGCCGTGTCATTTGATGGAAGagtgttgacaatgaaattgtgctgtggg
agccaaaaacaaaagaacaaagtcccggggaggtgttataattcaacttctctagaactacaga
ccttaatt

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 281
Transcript exons: 2081897-2081956,2082119-2082215,2082481-2082544,2082647-
2082706
AGTGTTGACAATGAAATTGTGCTGTGGGAGCCAAAAACAAAAGAACAAAGTCCCGGGGAGGGTA
GCATTGATATTCTTCAGAAGTATCCTGTGCCAGAATGTGATATCTGGTTTATCAAATTCTCATG
CGATTTTCACTTCAATCAATTGGCAATAGGCAACCGTGAAGGAAAAGTCTTTGTCTGGGAAGTA
CAGTCCAGTCCTCCTGTTTTAACTGCTCGGCTGACTAATCCGCAATGCAAATCTGCGATAAGGC
AGACTGCCGTGTCATTTGATGGAAG
						
						Genomic sequence
						
							AGTGTTGACAATGAAATTGTGCTGTGGGAGCCAAAAACAAAAGAACAAAGTCCCGGGGAGGTGT
TATAATTCAACTTCTCTAGAACTACAGACCTTAATTCATTTTACTTGTCTTCTGATCTGATCAT
CTCAACAGTTTTCTGTCATGTTACTTTAGTTTTCACTATCCCTTTCCATACGTCTCCCTCTCAA
GTAAAATTACTTTTTTTTTTTCAAATGTAGGGTAGCATTGATATTCTTCAGAAGTATCCTGTGC
CAGAATGTGATATCTGGTTTATCAAATTCTCATGCGATTTTCACTTCAATCAATTGGCAATAGG
TAATATCTCCCAAATTGTGTTGTTACTTTGGAAAATTTGTGACAATATTGAAAATGTTTTGTTT
GTGGGAAACGGTCATACTCAGTACTCACAAGTCAAAAGATTACATTCAATAAAAAGAATTGTGG
AATGTTGCCCAGCACCCTTAATGTTGTGACAAAAAAGAAACTAATTCACATATTTAAACTCATT
GGGTTTAGATTATTATTTTTAAACAAATACTACACTGAATACTCAAAGATAACCATGTGCTTAT
TTAAACAGGCAACCGTGAAGGAAAAGTCTTTGTCTGGGAAGTACAGTCCAGTCCTCCTGTTTTA
ACTGCTCGGTAAGTTCCAAGAACTTCAGTTGTTTCCCATCCTTTATATGCACTTGGTTTTGGAC
GCCACTAAATCTTGTATTTCTGCATGCCAACACATTATTTTTACAGGCTGACTAATCCGCAATG
CAAATCTGCGATAAGGCAGACTGCCGTGTCATTTGATGGAAG

Sequences
Splice junction sequence	AAATCTTGTATTTCTGCATGCCAACACATTATTTTTACAGGCTGACTAATCCGCAATGCAAATC TGCGATAAGGCAGACTGCCGTGTCATTTGATGGAAGagtgttgacaatgaaattgtgctgtggg agccaaaaacaaaagaacaaagtcccggggaggtgttataattcaacttctctagaactacaga ccttaatt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 281 Transcript exons: 2081897-2081956,2082119-2082215,2082481-2082544,2082647- 2082706 AGTGTTGACAATGAAATTGTGCTGTGGGAGCCAAAAACAAAAGAACAAAGTCCCGGGGAGGGTA GCATTGATATTCTTCAGAAGTATCCTGTGCCAGAATGTGATATCTGGTTTATCAAATTCTCATG CGATTTTCACTTCAATCAATTGGCAATAGGCAACCGTGAAGGAAAAGTCTTTGTCTGGGAAGTA CAGTCCAGTCCTCCTGTTTTAACTGCTCGGCTGACTAATCCGCAATGCAAATCTGCGATAAGGC AGACTGCCGTGTCATTTGATGGAAG
Genomic sequence	AGTGTTGACAATGAAATTGTGCTGTGGGAGCCAAAAACAAAAGAACAAAGTCCCGGGGAGGTGT TATAATTCAACTTCTCTAGAACTACAGACCTTAATTCATTTTACTTGTCTTCTGATCTGATCAT CTCAACAGTTTTCTGTCATGTTACTTTAGTTTTCACTATCCCTTTCCATACGTCTCCCTCTCAA GTAAAATTACTTTTTTTTTTTCAAATGTAGGGTAGCATTGATATTCTTCAGAAGTATCCTGTGC CAGAATGTGATATCTGGTTTATCAAATTCTCATGCGATTTTCACTTCAATCAATTGGCAATAGG TAATATCTCCCAAATTGTGTTGTTACTTTGGAAAATTTGTGACAATATTGAAAATGTTTTGTTT GTGGGAAACGGTCATACTCAGTACTCACAAGTCAAAAGATTACATTCAATAAAAAGAATTGTGG AATGTTGCCCAGCACCCTTAATGTTGTGACAAAAAAGAAACTAATTCACATATTTAAACTCATT GGGTTTAGATTATTATTTTTAAACAAATACTACACTGAATACTCAAAGATAACCATGTGCTTAT TTAAACAGGCAACCGTGAAGGAAAAGTCTTTGTCTGGGAAGTACAGTCCAGTCCTCCTGTTTTA ACTGCTCGGTAAGTTCCAAGAACTTCAGTTGTTTCCCATCCTTTATATGCACTTGGTTTTGGAC GCCACTAAATCTTGTATTTCTGCATGCCAACACATTATTTTTACAGGCTGACTAATCCGCAATG CAAATCTGCGATAAGGCAGACTGCCGTGTCATTTGATGGAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.237488889

Conservation Information
Conserved circRNAs	NA
PMCS	0.237488889

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							2082128-2082654(-)
						
						Potential amino acid sequence
						
							MLPSPGLCSFVFGSHSTISLSTLFHQMTRQSALSQICIAD*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	2082128-2082654(-)
Potential amino acid sequence	MLPSPGLCSFVFGSHSTISLSTLFHQMTRQSALSQICIAD*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os08g0137100_circ_g.3

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