Plant Circular RNA Database

Detail information of Os03g0619400_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os03g0619400_circ_g.2
					
						ID in PlantcircBase
						osa_circ_020668
					
						Alias
						Os_ciR8673
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 23487049-23488299  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os03g0619400						
					
						Parent gene annotation
						
							Chaperone, tailless complex polypeptide 1 domain containing prot
ein. (Os03t0619400-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os03g0619400_circ_g.1 Os03g0619400_circ_g.3 Os03g0619400_circ_g.4 Os03g0619400_circ_g.5 Os03g0619400_circ_g.6 						
					
						Support reads
						2/1
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0619400-01:5

General Information
CircRNA Name	Os03g0619400_circ_g.2
ID in PlantcircBase	osa_circ_020668
Alias	Os_ciR8673
Organism	Oryza sativa
Position	chr3: 23487049-23488299 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os03g0619400
Parent gene annotation	Chaperone, tailless complex polypeptide 1 domain containing prot ein. (Os03t0619400-01)
Parent gene strand	+
Alternative splicing	Os03g0619400_circ_g.1 Os03g0619400_circ_g.3 Os03g0619400_circ_g.4 Os03g0619400_circ_g.5 Os03g0619400_circ_g.6
Support reads	2/1
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0619400-01:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							AATGCTCCATTTCTTTGTGAAACTATAGGTTACAGAATGGCTGTTGAGTGTGCTAGAAACGCTT
TGCTGGAGAGGACCATGGACAACAAAGAAAATATAGgtaaatggagagggtgctgaaggatgat
gctgttgaggagaagggcgaacgtgccaggatggtgtgaaaaccctttcttatcgatttgacct
ttttaatt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 440
Transcript exons: 23487049-23487109,23487217-23487282,23487370-23487481,23
487673-23487801,23488228-23488299
GTAAATGGAGAGGGTGCTGAAGGATGATGCTGTTGAGGAGAAGGGCGAACGTGCCAGGATGGCA
GCCTTCATTGGTGCTATGGCTATTGCAGATTTGGTCAAGACTACATTGGGACCTAAAGGAATGG
ACAAGATCTTGCAATCAACAGGCAGAGGGAGGAGTGTTACTGTTACAAATGATGGGGCTACAAT
TTTGAAGTCCCTTCATATAGACAACCCTGCTGCAAAGGTCCTTGTTGACATCTCAAAAGTTCAA
GATGATGAAGTTGGTGACGGGACAACCTCTGTGGTTGTTTTGGCTGGAGAACTTTTGAGGGAGG
CTGAAAAGTTAGTTAACATGAAAATTCATCCAATGACTATAATTGCAGGTTACAGAATGGCTGT
TGAGTGTGCTAGAAACGCTTTGCTGGAGAGGACCATGGACAACAAAGAAAATATAG
						
					

						Genomic sequence
						
							GTAAATGGAGAGGGTGCTGAAGGATGATGCTGTTGAGGAGAAGGGCGAACGTGCCAGGATGGTG
TGAAAACCCTTTCTTATCGATTTGACCTTTTTAATTGGTTTTTCTTAGTTAGTTGTATAGATCC
TGCAATTTTACACAACAACCTGGTGTCTTTAAATTGGCAGGCAGCCTTCATTGGTGCTATGGCT
ATTGCAGATTTGGTCAAGACTACATTGGGACCTAAAGGAATGGTATGCTCCTTAGTGCTAGACT
GCTAGTGCTTGAAGTACATGATTCCAGTGTAGTAAATTTTTACATGTGGCAATATTTATGTTCA
GGACAAGATCTTGCAATCAACAGGCAGAGGGAGGAGTGTTACTGTTACAAATGATGGGGCTACA
ATTTTGAAGTCCCTTCATATAGACAACCCTGCTGCAAAGGTCCTTGTTGGTATCCTTTCATTTG
AATCTGTTGCTTGATGATTTTTCATACTAACACAATAGCAAGTCATAGATGAAGTGGCAAAGGG
GCAAGTTTCCTTTATACAGCAACATGCGTACTTATAGTTTCTCATGCTACCTCATTTGAGTATT
TGATATGTATGATGTATCAAGGAAGTTAAGTCCTTAACGCATTGTTAGACATCTCAAAAGTTCA
AGATGATGAAGTTGGTGACGGGACAACCTCTGTGGTTGTTTTGGCTGGAGAACTTTTGAGGGAG
GCTGAAAAGTTAGTTAACATGAAAATTCATCCAATGACTATAATTGCAGGTAATTCTCATCGTT
GGTTTAATATGCTTTCCAGGCTCACCATATTTTGCATGATTTGTATAATATGGTATTCCTAACA
TATCAAACTAGGTTAACTCAATTAATTAGTCACCTCTTCAATATGTGTAAATTGGTTTGTTGAG
GGTAAAATTCTCAACACGCACTAGTTCATGTACTACTATAAATCTATATTACAATTGACAATAG
AAGTGCGTTGAGATTTTCGCAAGGACAATACAACAGATGGAAGATTGCTTGGTTGGTTTGAAAA
TTGGAAGTTAGCATTCTTAAATAGTTGGTTGAATGCAGTTTGTTCATATGCATAGGCTGAATTC
AGATTTTTCACTGCACCAACAAGAAAAACCATTATGTGAATTCATGAGTACACTAACATCTCCA
ATGCTCCATTTCTTTGTGAAACTATAGGTTACAGAATGGCTGTTGAGTGTGCTAGAAACGCTTT
GCTGGAGAGGACCATGGACAACAAAGAAAATATAG
						
					

			
		

Sequences
Splice junction sequence	AATGCTCCATTTCTTTGTGAAACTATAGGTTACAGAATGGCTGTTGAGTGTGCTAGAAACGCTT TGCTGGAGAGGACCATGGACAACAAAGAAAATATAGgtaaatggagagggtgctgaaggatgat gctgttgaggagaagggcgaacgtgccaggatggtgtgaaaaccctttcttatcgatttgacct ttttaatt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 440 Transcript exons: 23487049-23487109,23487217-23487282,23487370-23487481,23 487673-23487801,23488228-23488299 GTAAATGGAGAGGGTGCTGAAGGATGATGCTGTTGAGGAGAAGGGCGAACGTGCCAGGATGGCA GCCTTCATTGGTGCTATGGCTATTGCAGATTTGGTCAAGACTACATTGGGACCTAAAGGAATGG ACAAGATCTTGCAATCAACAGGCAGAGGGAGGAGTGTTACTGTTACAAATGATGGGGCTACAAT TTTGAAGTCCCTTCATATAGACAACCCTGCTGCAAAGGTCCTTGTTGACATCTCAAAAGTTCAA GATGATGAAGTTGGTGACGGGACAACCTCTGTGGTTGTTTTGGCTGGAGAACTTTTGAGGGAGG CTGAAAAGTTAGTTAACATGAAAATTCATCCAATGACTATAATTGCAGGTTACAGAATGGCTGT TGAGTGTGCTAGAAACGCTTTGCTGGAGAGGACCATGGACAACAAAGAAAATATAG
Genomic sequence	GTAAATGGAGAGGGTGCTGAAGGATGATGCTGTTGAGGAGAAGGGCGAACGTGCCAGGATGGTG TGAAAACCCTTTCTTATCGATTTGACCTTTTTAATTGGTTTTTCTTAGTTAGTTGTATAGATCC TGCAATTTTACACAACAACCTGGTGTCTTTAAATTGGCAGGCAGCCTTCATTGGTGCTATGGCT ATTGCAGATTTGGTCAAGACTACATTGGGACCTAAAGGAATGGTATGCTCCTTAGTGCTAGACT GCTAGTGCTTGAAGTACATGATTCCAGTGTAGTAAATTTTTACATGTGGCAATATTTATGTTCA GGACAAGATCTTGCAATCAACAGGCAGAGGGAGGAGTGTTACTGTTACAAATGATGGGGCTACA ATTTTGAAGTCCCTTCATATAGACAACCCTGCTGCAAAGGTCCTTGTTGGTATCCTTTCATTTG AATCTGTTGCTTGATGATTTTTCATACTAACACAATAGCAAGTCATAGATGAAGTGGCAAAGGG GCAAGTTTCCTTTATACAGCAACATGCGTACTTATAGTTTCTCATGCTACCTCATTTGAGTATT TGATATGTATGATGTATCAAGGAAGTTAAGTCCTTAACGCATTGTTAGACATCTCAAAAGTTCA AGATGATGAAGTTGGTGACGGGACAACCTCTGTGGTTGTTTTGGCTGGAGAACTTTTGAGGGAG GCTGAAAAGTTAGTTAACATGAAAATTCATCCAATGACTATAATTGCAGGTAATTCTCATCGTT GGTTTAATATGCTTTCCAGGCTCACCATATTTTGCATGATTTGTATAATATGGTATTCCTAACA TATCAAACTAGGTTAACTCAATTAATTAGTCACCTCTTCAATATGTGTAAATTGGTTTGTTGAG GGTAAAATTCTCAACACGCACTAGTTCATGTACTACTATAAATCTATATTACAATTGACAATAG AAGTGCGTTGAGATTTTCGCAAGGACAATACAACAGATGGAAGATTGCTTGGTTGGTTTGAAAA TTGGAAGTTAGCATTCTTAAATAGTTGGTTGAATGCAGTTTGTTCATATGCATAGGCTGAATTC AGATTTTTCACTGCACCAACAAGAAAAACCATTATGTGAATTCATGAGTACACTAACATCTCCA ATGCTCCATTTCTTTGTGAAACTATAGGTTACAGAATGGCTGTTGAGTGTGCTAGAAACGCTTT GCTGGAGAGGACCATGGACAACAAAGAAAATATAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.272826399

Conservation Information
Conserved circRNAs	NA
PMCS	0.272826399

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							23487054-23487065(+)
23487229-23488254(-)
						
						Potential amino acid sequence
						
							MERVLKDDAVEEKGERARMAAFIGAMAIADLVKTTLGPKGMDKILQSTGRGRSVTVTNDGATIL
KSLHIDNPAAKVLVDISKVQDDEVGDGTTSVVVLAGELLREAEKLVNMKIHPMTIIAGYRMAVE
CARNALLERTMDNKENIGKWRGC*(+)
MKAAILARSPFSSTASSFSTLSIYLYFLCCPWSSPAKRF*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	23487054-23487065(+) 23487229-23488254(-)
Potential amino acid sequence	MERVLKDDAVEEKGERARMAAFIGAMAIADLVKTTLGPKGMDKILQSTGRGRSVTVTNDGATIL KSLHIDNPAAKVLVDISKVQDDEVGDGTTSVVVLAGELLREAEKLVNMKIHPMTIIAGYRMAVE CARNALLERTMDNKENIGKWRGC(+) MKAAILARSPFSSTASSFSTLSIYLYFLCCPWSSPAKRF(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os03g0619400_circ_g.2

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