Plant Circular RNA Database

Detail information of AT2G46270_circ_g.2

			
						 General Information
					
						CircRNA Name
						AT2G46270_circ_g.2
					
						ID in PlantcircBase
						ath_circ_018676
					
						Alias
						AT2G46270_C1, AT2G46270_C1
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr2: 19001012-19001411  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI2
					
						Parent gene
						
							AT2G46270						
					
						Parent gene annotation
						
							G-box-binding factor 3
							
						Parent gene strand
						+
					
						Alternative splicing
						
							2_circ_ag.4 AT2G46070_circ_g.1 AT2G46090_circ_g.1 AT2G46090_circ_g.2 AT2G46090_circ_g.3 AT2G46100_circ_g.1 AT2G46100_circ_g.2 AT2G46140_circ_g.1 AT2G46170_circ_g.1 AT2G46180_circ_g.1 AT2G46180_circ_g.2 AT2G46180_circ_g.3 AT2G46180_circ_g.4 AT2G46180_circ_g.5 AT2G46180_circ_g.6 AT2G46180_circ_g.7 AT2G46180_circ_g.8 AT2G46192_circ_g.1 AT2G46192_circ_g.2 ENSRNA049495738_circ_g.1 AT2G46200_circ_g.1 AT2G46225_circ_g.1 AT2G46225_circ_g.2 AT2G46225_circ_g.3 AT2G46225_circ_g.4 AT2G46230_circ_g.1 AT2G46230_circ_g.2 AT2G46230_circ_g.3 2_circ_igg.4 AT2G46240_circ_g.1 AT2G46240_circ_g.2 AT2G46260_circ_g.1 AT2G46260_circ_g.2 AT2G46270_circ_g.1 AT2G46270_circ_g.3 AT2G46270_circ_g.4 AT2G46280_circ_g.1 2_circ_ag.2 AT2G46280_circ_g.3 2_circ_ag.4 AT2G46280_circ_g.5 2_circ_ag.6 2_circ_ag.7 AT2G46280_circ_g.8 2_circ_ag.9 2_circ_ag.10 AT2G46280_circ_g.11 2_circ_ag.12 AT2G46320_circ_g.1 AT2G46320_circ_g.2 AT2G46320_circ_g.3 AT2G46320_circ_g.4 AT2G46330_circ_g.1 AT2G46340_circ_g.1 AT2G46340_circ_g.2 AT2G46340_circ_g.3 AT2G46340_circ_g.4 AT2G46340_circ_g.5 AT2G46340_circ_g.6 AT2G46340_circ_g.7 AT2G46340_circ_g.8 						
					
						Support reads
						NA
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT2G46270.3:3
AT2G46270.4:3
AT2G46270.2:3
AT2G46270.1:3

General Information
CircRNA Name	AT2G46270_circ_g.2
ID in PlantcircBase	ath_circ_018676
Alias	AT2G46270_C1, AT2G46270_C1
Organism	Arabidpsis thaliana
Position	chr2: 19001012-19001411 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRI2
Parent gene	AT2G46270
Parent gene annotation	G-box-binding factor 3
Parent gene strand	+
Alternative splicing	2_circ_ag.4 AT2G46070_circ_g.1 AT2G46090_circ_g.1 AT2G46090_circ_g.2 AT2G46090_circ_g.3 AT2G46100_circ_g.1 AT2G46100_circ_g.2 AT2G46140_circ_g.1 AT2G46170_circ_g.1 AT2G46180_circ_g.1 AT2G46180_circ_g.2 AT2G46180_circ_g.3 AT2G46180_circ_g.4 AT2G46180_circ_g.5 AT2G46180_circ_g.6 AT2G46180_circ_g.7 AT2G46180_circ_g.8 AT2G46192_circ_g.1 AT2G46192_circ_g.2 ENSRNA049495738_circ_g.1 AT2G46200_circ_g.1 AT2G46225_circ_g.1 AT2G46225_circ_g.2 AT2G46225_circ_g.3 AT2G46225_circ_g.4 AT2G46230_circ_g.1 AT2G46230_circ_g.2 AT2G46230_circ_g.3 2_circ_igg.4 AT2G46240_circ_g.1 AT2G46240_circ_g.2 AT2G46260_circ_g.1 AT2G46260_circ_g.2 AT2G46270_circ_g.1 AT2G46270_circ_g.3 AT2G46270_circ_g.4 AT2G46280_circ_g.1 2_circ_ag.2 AT2G46280_circ_g.3 2_circ_ag.4 AT2G46280_circ_g.5 2_circ_ag.6 2_circ_ag.7 AT2G46280_circ_g.8 2_circ_ag.9 2_circ_ag.10 AT2G46280_circ_g.11 2_circ_ag.12 AT2G46320_circ_g.1 AT2G46320_circ_g.2 AT2G46320_circ_g.3 AT2G46320_circ_g.4 AT2G46330_circ_g.1 AT2G46340_circ_g.1 AT2G46340_circ_g.2 AT2G46340_circ_g.3 AT2G46340_circ_g.4 AT2G46340_circ_g.5 AT2G46340_circ_g.6 AT2G46340_circ_g.7 AT2G46340_circ_g.8
Support reads	NA
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT2G46270.3:3 AT2G46270.4:3 AT2G46270.2:3 AT2G46270.1:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CTTAAATCCCTTTCAGCATATGATGTCACCATATGGAGCACCCTATGCTGCTGTTTATCCTCAT
GGAGGAGGAGTTTACGCTCATCCCGGTATTCCCATGgatcaaacaaatgttcatgtctaccctg
attgggcagctatgcaggttagtcttttttttattacgctccagttatgtactagtaataccat
gattaata

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 225
Transcript exons: 19001012-19001056,19001139-19001234,19001328-19001411
GATCAAACAAATGTTCATGTCTACCCTGATTGGGCAGCTATGCAGGCATATTATGGTCCAAGAG
TAGCAATGCCTCCTTATTACAATTCAGCTATGGCTGCATCTGGTCATCCTCCTCCTCCTTACAT
GTGGAATCCTCAGCATATGATGTCACCATATGGAGCACCCTATGCTGCTGTTTATCCTCATGGA
GGAGGAGTTTACGCTCATCCCGGTATTCCCATG
						
						Genomic sequence
						
							GATCAAACAAATGTTCATGTCTACCCTGATTGGGCAGCTATGCAGGTTAGTCTTTTTTTTATTA
CGCTCCAGTTATGTACTAGTAATACCATGATTAATAATGATTTTCATGATGTGTTGCTGCTAGG
CATATTATGGTCCAAGAGTAGCAATGCCTCCTTATTACAATTCAGCTATGGCTGCATCTGGTCA
TCCTCCTCCTCCTTACATGTGGAATCCTCAGGTACTAAACACAAACACGAATCATCTTGTGCTA
ATATGGTAAAGATTAGGAAGACGTTTCATTGGTGTTTTGTTGGTCTTAAATCCCTTTCAGCATA
TGATGTCACCATATGGAGCACCCTATGCTGCTGTTTATCCTCATGGAGGAGGAGTTTACGCTCA
TCCCGGTATTCCCATG

Sequences
Splice junction sequence	CTTAAATCCCTTTCAGCATATGATGTCACCATATGGAGCACCCTATGCTGCTGTTTATCCTCAT GGAGGAGGAGTTTACGCTCATCCCGGTATTCCCATGgatcaaacaaatgttcatgtctaccctg attgggcagctatgcaggttagtcttttttttattacgctccagttatgtactagtaataccat gattaata
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 225 Transcript exons: 19001012-19001056,19001139-19001234,19001328-19001411 GATCAAACAAATGTTCATGTCTACCCTGATTGGGCAGCTATGCAGGCATATTATGGTCCAAGAG TAGCAATGCCTCCTTATTACAATTCAGCTATGGCTGCATCTGGTCATCCTCCTCCTCCTTACAT GTGGAATCCTCAGCATATGATGTCACCATATGGAGCACCCTATGCTGCTGTTTATCCTCATGGA GGAGGAGTTTACGCTCATCCCGGTATTCCCATG
Genomic sequence	GATCAAACAAATGTTCATGTCTACCCTGATTGGGCAGCTATGCAGGTTAGTCTTTTTTTTATTA CGCTCCAGTTATGTACTAGTAATACCATGATTAATAATGATTTTCATGATGTGTTGCTGCTAGG CATATTATGGTCCAAGAGTAGCAATGCCTCCTTATTACAATTCAGCTATGGCTGCATCTGGTCA TCCTCCTCCTCCTTACATGTGGAATCCTCAGGTACTAAACACAAACACGAATCATCTTGTGCTA ATATGGTAAAGATTAGGAAGACGTTTCATTGGTGTTTTGTTGGTCTTAAATCCCTTTCAGCATA TGATGTCACCATATGGAGCACCCTATGCTGCTGTTTATCCTCATGGAGGAGGAGTTTACGCTCA TCCCGGTATTCCCATG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.244577667

Conservation Information
Conserved circRNAs	NA
PMCS	0.244577667

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							19001052-19001408(+)
19001345-19001157(+)
						
						Potential amino acid sequence
						
							MQAYYGPRVAMPPYYNSAMAASGHPPPPYMWNPQHMMSPYGAPYAAVYPHGGGVYAHPGIPMDQ
TNVHVYPDWAAMQAYYGPRVAMPPYYNSAMAASGHPPPPYMWNPQHMMSPYGAPYAAVYPHGGG
VYAHPGIPMDQTNVHVYPDWAAMQAYYGPRVAMPPYYNSAMAASGHPPPPYMWNPQHMMSPYGA
PYAAVYPHGGGVYAHPGIPMDQTNVHVYPDWAAMQAYYGPRVAMPPYYNSAMAASGHPPPPYMW
NPQHMMSPYGAPYAAVYPHGGGVYAHPGIPM
MEHPMLLFILMEEEFTLIPVFPWIKQMFMSTLIGQLCRHIMVQE*
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						response to drought stress

Functional Information
Coding potential	Y
Potential coding position	19001052-19001408(+) 19001345-19001157(+)
Potential amino acid sequence	MQAYYGPRVAMPPYYNSAMAASGHPPPPYMWNPQHMMSPYGAPYAAVYPHGGGVYAHPGIPMDQ TNVHVYPDWAAMQAYYGPRVAMPPYYNSAMAASGHPPPPYMWNPQHMMSPYGAPYAAVYPHGGG VYAHPGIPMDQTNVHVYPDWAAMQAYYGPRVAMPPYYNSAMAASGHPPPPYMWNPQHMMSPYGA PYAAVYPHGGGVYAHPGIPMDQTNVHVYPDWAAMQAYYGPRVAMPPYYNSAMAASGHPPPPYMW NPQHMMSPYGAPYAAVYPHGGGVYAHPGIPM MEHPMLLFILMEEEFTLIPVFPWIKQMFMSTLIGQLCRHIMVQE*
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	response to drought stress

			
						 Other Information
					
						References
						Zhang et al., 2019

circRNA

Detail information of AT2G46270_circ_g.2

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