Plant Circular RNA Database

Detail information of AT4G34640_circ_g.10

			
						 General Information
					
						CircRNA Name
						AT4G34640_circ_g.10
					
						ID in PlantcircBase
						ath_circ_034683
					
						Alias
						At_ciR5080, Ath_circ_FC2997, Ath_circ_FC2998
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr4: 16539996-16541296  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						PcircRNA_finder, find_circ
					
						Parent gene
						
							AT4G34640						
					
						Parent gene annotation
						
							Squalene synthase 1
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT4G34640_circ_g.1 AT4G34640_circ_g.2 AT4G34640_circ_g.3 AT4G34640_circ_g.4 4_circ_ag.5 AT4G34640_circ_g.6 AT4G34640_circ_g.7 4_circ_ag.8 AT4G34640_circ_g.9 						
					
						Support reads
						2/9
					
						Tissues
						leaf/whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT4G34640.2:6
AT4G34640.1:6

General Information
CircRNA Name	AT4G34640_circ_g.10
ID in PlantcircBase	ath_circ_034683
Alias	At_ciR5080, Ath_circ_FC2997, Ath_circ_FC2998
Organism	Arabidpsis thaliana
Position	chr4: 16539996-16541296 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	PcircRNA_finder, find_circ
Parent gene	AT4G34640
Parent gene annotation	Squalene synthase 1
Parent gene strand	+
Alternative splicing	AT4G34640_circ_g.1 AT4G34640_circ_g.2 AT4G34640_circ_g.3 AT4G34640_circ_g.4 4_circ_ag.5 AT4G34640_circ_g.6 AT4G34640_circ_g.7 4_circ_ag.8 AT4G34640_circ_g.9
Support reads	2/9
Tissues	leaf/whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT4G34640.2:6 AT4G34640.1:6

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TGACATAGGTTGACAAGAACGATCCAAATGCCAGTAAGACACTAAACCGACTTGAAGCCGTTCA
GAAACTCTGCAGAGACGCTGGAGTTCTTCAAAACAGgtagaaactgttgatgactacgatgaat
actgccactatgttgctgggcttgttggtttaggtttgtcgaaactcttcctcgctgcaggatc
agaggttt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 659
Transcript exons: 16539996-16540145,16540254-16540358,16540461-16540607,16
540783-16540858,16541019-16541107,16541205-16541296
GTAGAAACTGTTGATGACTACGATGAATACTGCCACTATGTTGCTGGGCTTGTTGGTTTAGGTT
TGTCGAAACTCTTCCTCGCTGCAGGATCAGAGGTTTTGACACCAGATTGGGAGGCGATTTCCAA
TTCAATGGGTTTATTTCTGCAGAAAACAAACATTATCAGAGATTATCTTGAGGACATTAATGAG
ATACCAAAATCCCGCATGTTTTGGCCTCGCGAGATTTGGGGCAAATATGCTGACAAGCTTGAGG
ATTTAAAATACGAGGAGAACACAAACAAATCCGTACAGTGCTTAAATGAAATGGTTACCAATGC
GTTGATGCATATTGAAGATTGCCTGAAATACATGGTTTCCTTGCGTGATCCTTCCATATTTCGG
TTCTGTGCCATCCCTCAGATCATGGCGATTGGAACACTTGCATTATGCTATAACAATGAACAAG
TATTCAGAGGCGTTGTGAAACTGAGGCGAGGTCTTACTGCTAAAGTCATTGATCGTACAAAGAC
AATGGCTGATGTCTATGGTGCTTTCTATGATTTTTCCTGCATGCTGAAGACAAAGGTTGACAAG
AACGATCCAAATGCCAGTAAGACACTAAACCGACTTGAAGCCGTTCAGAAACTCTGCAGAGACG
CTGGAGTTCTTCAAAACAG
						
					

						Genomic sequence
						
							GTAGAAACTGTTGATGACTACGATGAATACTGCCACTATGTTGCTGGGCTTGTTGGTTTAGGTT
TGTCGAAACTCTTCCTCGCTGCAGGATCAGAGGTTTTGACACCAGATTGGGAGGCGATTTCCAA
TTCAATGGGTTTATTTCTGCAGGTACAACAATGTATTTTGAGGTGTACAATGTTTCTTCTTCCT
CAAATCTTTTGCAATTTCGGACAATTTCTATCATTGAATGATTTATAACACTGGTCTTATTTGC
AGAAAACAAACATTATCAGAGATTATCTTGAGGACATTAATGAGATACCAAAATCCCGCATGTT
TTGGCCTCGCGAGATTTGGGGCAAATATGCTGACAAGCTTGAGGTGCATACATACCACTCTCAA
GCTCTCATCTTAGGTCTGATGTTACCCATATTGACTATTTTCTGACAGTGCCTTTTTGGTGATT
TGTAAATTGTAATGTAGGATTTAAAATACGAGGAGAACACAAACAAATCCGTACAGTGCTTAAA
TGAAATGGTTACCAATGCGTTGATGCATATTGAAGATTGCCTGAAATACATGGTTTCCTTGCGT
GATCCTTCCATATTTCGGTTCTGTGCCATCCCTCAGGTTAGAGAACGAAATATCACATAATTGT
TAAGTCTTTAAACTTCACATTGAGAACTGTTTATATTGTTTTCCTCTTCATATGGAATTAGGTA
TTCTCCTCTTTAACTACGTCTGCACTAATATAGAATGCCTTAGAAGTTAATATTGCTTTGCTTG
GGTGTATTTTCTTGATTAGATCATGGCGATTGGAACACTTGCATTATGCTATAACAATGAACAA
GTATTCAGAGGCGTTGTGAAACTGAGGCGAGGTAAGATGTTAAGCCTTAGAAATTATGTAGGCC
AATCCCTAATTTCTATTTATGAGAAAGGAAAACCCGTTATCTTTTATTATCAACGAGCTTGAGA
ACGTGTAGTAGCTAGCCTTATTATCTGAAGGCTAATATCGCTGAACAACTATCTATTGTTTAGG
TCTTACTGCTAAAGTCATTGATCGTACAAAGACAATGGCTGATGTCTATGGTGCTTTCTATGAT
TTTTCCTGCATGCTGAAGACAAAGGTAGTCATCAGTTCCATGTTTGTGATTAGTTTTTCACTCA
GTTTCTGTTTAAAGAAAAGAATCATATGTGAATTTTCTATCGACATATATGACATAGGTTGACA
AGAACGATCCAAATGCCAGTAAGACACTAAACCGACTTGAAGCCGTTCAGAAACTCTGCAGAGA
CGCTGGAGTTCTTCAAAACAG
						
					

			
		

Sequences
Splice junction sequence	TGACATAGGTTGACAAGAACGATCCAAATGCCAGTAAGACACTAAACCGACTTGAAGCCGTTCA GAAACTCTGCAGAGACGCTGGAGTTCTTCAAAACAGgtagaaactgttgatgactacgatgaat actgccactatgttgctgggcttgttggtttaggtttgtcgaaactcttcctcgctgcaggatc agaggttt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 659 Transcript exons: 16539996-16540145,16540254-16540358,16540461-16540607,16 540783-16540858,16541019-16541107,16541205-16541296 GTAGAAACTGTTGATGACTACGATGAATACTGCCACTATGTTGCTGGGCTTGTTGGTTTAGGTT TGTCGAAACTCTTCCTCGCTGCAGGATCAGAGGTTTTGACACCAGATTGGGAGGCGATTTCCAA TTCAATGGGTTTATTTCTGCAGAAAACAAACATTATCAGAGATTATCTTGAGGACATTAATGAG ATACCAAAATCCCGCATGTTTTGGCCTCGCGAGATTTGGGGCAAATATGCTGACAAGCTTGAGG ATTTAAAATACGAGGAGAACACAAACAAATCCGTACAGTGCTTAAATGAAATGGTTACCAATGC GTTGATGCATATTGAAGATTGCCTGAAATACATGGTTTCCTTGCGTGATCCTTCCATATTTCGG TTCTGTGCCATCCCTCAGATCATGGCGATTGGAACACTTGCATTATGCTATAACAATGAACAAG TATTCAGAGGCGTTGTGAAACTGAGGCGAGGTCTTACTGCTAAAGTCATTGATCGTACAAAGAC AATGGCTGATGTCTATGGTGCTTTCTATGATTTTTCCTGCATGCTGAAGACAAAGGTTGACAAG AACGATCCAAATGCCAGTAAGACACTAAACCGACTTGAAGCCGTTCAGAAACTCTGCAGAGACG CTGGAGTTCTTCAAAACAG
Genomic sequence	GTAGAAACTGTTGATGACTACGATGAATACTGCCACTATGTTGCTGGGCTTGTTGGTTTAGGTT TGTCGAAACTCTTCCTCGCTGCAGGATCAGAGGTTTTGACACCAGATTGGGAGGCGATTTCCAA TTCAATGGGTTTATTTCTGCAGGTACAACAATGTATTTTGAGGTGTACAATGTTTCTTCTTCCT CAAATCTTTTGCAATTTCGGACAATTTCTATCATTGAATGATTTATAACACTGGTCTTATTTGC AGAAAACAAACATTATCAGAGATTATCTTGAGGACATTAATGAGATACCAAAATCCCGCATGTT TTGGCCTCGCGAGATTTGGGGCAAATATGCTGACAAGCTTGAGGTGCATACATACCACTCTCAA GCTCTCATCTTAGGTCTGATGTTACCCATATTGACTATTTTCTGACAGTGCCTTTTTGGTGATT TGTAAATTGTAATGTAGGATTTAAAATACGAGGAGAACACAAACAAATCCGTACAGTGCTTAAA TGAAATGGTTACCAATGCGTTGATGCATATTGAAGATTGCCTGAAATACATGGTTTCCTTGCGT GATCCTTCCATATTTCGGTTCTGTGCCATCCCTCAGGTTAGAGAACGAAATATCACATAATTGT TAAGTCTTTAAACTTCACATTGAGAACTGTTTATATTGTTTTCCTCTTCATATGGAATTAGGTA TTCTCCTCTTTAACTACGTCTGCACTAATATAGAATGCCTTAGAAGTTAATATTGCTTTGCTTG GGTGTATTTTCTTGATTAGATCATGGCGATTGGAACACTTGCATTATGCTATAACAATGAACAA GTATTCAGAGGCGTTGTGAAACTGAGGCGAGGTAAGATGTTAAGCCTTAGAAATTATGTAGGCC AATCCCTAATTTCTATTTATGAGAAAGGAAAACCCGTTATCTTTTATTATCAACGAGCTTGAGA ACGTGTAGTAGCTAGCCTTATTATCTGAAGGCTAATATCGCTGAACAACTATCTATTGTTTAGG TCTTACTGCTAAAGTCATTGATCGTACAAAGACAATGGCTGATGTCTATGGTGCTTTCTATGAT TTTTCCTGCATGCTGAAGACAAAGGTAGTCATCAGTTCCATGTTTGTGATTAGTTTTTCACTCA GTTTCTGTTTAAAGAAAAGAATCATATGTGAATTTTCTATCGACATATATGACATAGGTTGACA AGAACGATCCAAATGCCAGTAAGACACTAAACCGACTTGAAGCCGTTCAGAAACTCTGCAGAGA CGCTGGAGTTCTTCAAAACAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.282601437

Conservation Information
Conserved circRNAs	NA
PMCS	0.282601437

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017;Chen et al., 2017a

circRNA

Detail information of AT4G34640_circ_g.10

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