Plant Circular RNA Database

Detail information of Os03g0254800_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os03g0254800_circ_g.4
					
						ID in PlantcircBase
						osa_circ_018914
					
						Alias
						Os_ciR1873
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 8178732-8181019  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os03g0254800						
					
						Parent gene annotation
						
							Similar to Chorismate synthase 1, chloroplast precursor (EC 4.2.
3.5) (5- enolpyruvylshikimate-3-phosphate phospholyase 1). (Os03
t0254800-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os03g0254800_circ_g.1 Os03g0254800_circ_g.2 Os03g0254800_circ_g.3 Os03g0254800_circ_g.5 Os03g0254800_circ_g.6 Os03g0254800_circ_g.7 Os03g0254800_circ_g.8 Os03g0254800_circ_g.9 Os03g0254800_circ_g.10 Os03g0254800_circ_g.11 Os03g0254800_circ_g.12 						
					
						Support reads
						6/1
					
						Tissues
						root/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0254800-01:10

General Information
CircRNA Name	Os03g0254800_circ_g.4
ID in PlantcircBase	osa_circ_018914
Alias	Os_ciR1873
Organism	Oryza sativa
Position	chr3: 8178732-8181019 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os03g0254800
Parent gene annotation	Similar to Chorismate synthase 1, chloroplast precursor (EC 4.2. 3.5) (5- enolpyruvylshikimate-3-phosphate phospholyase 1). (Os03 t0254800-01)
Parent gene strand	-
Alternative splicing	Os03g0254800_circ_g.1 Os03g0254800_circ_g.2 Os03g0254800_circ_g.3 Os03g0254800_circ_g.5 Os03g0254800_circ_g.6 Os03g0254800_circ_g.7 Os03g0254800_circ_g.8 Os03g0254800_circ_g.9 Os03g0254800_circ_g.10 Os03g0254800_circ_g.11 Os03g0254800_circ_g.12
Support reads	6/1
Tissues	root/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0254800-01:10

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GGGCATCCACTGATTACGCAACCAACACCGCCTCCATGAGACTCTCCATAAGTTGCAACCTGGA
AGTAGTTCCCAAATACATTTGCAGACGCCTTCACCTcccaatagtcgcggttggcttgaaagct
actttgaagtatataatttcaccatttgatatccctccctgcatggacatacacattataaata
ctaagaca

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 941
Transcript exons: 8178732-8178797,8178938-8179032,8179141-8179243,8179457-
8179588,8179677-8179757,8179959-8180051,8180208-8180291,
8180472-8180530,8180635-8180711,8180869-8181019
CCCAATAGTCGCGGTTGGCTTGAAAGCTACTTTGAAGTATATAATTTCACCATTTGATATCCCT
CCCTGCACACCGCCTGAACGATTAGTTCTTGTTCTCACATTTCCAGCCTCATCCATATAGAACT
CATCATTATGCTCACTTCCAGTGTAGTCAGTACCTGCAAATCCACTGCCGATCTCAAACCCCTT
GCTTGCAGGAAGAGAAAGCATAGCTTTCGCCAATTCAGCCTCAAGTTTGTCAAATACAGGAGAG
CCAATCCCACGAGGAACATTTCTTGCAATGCATGTGACCACACCACCAATCGAATCACCTCTAA
CTCGTACTTTATCGATTGCATCAATCATCTTCTGTGCATATTCTGGATCAGGACATCTAACAAT
GTTGCTTTCTATCTGTTCCATTGTTACAGTGTCATAATCAACGGCATCTTCTGGTAGTACAACT
TGATGCACCTTGGACACAAACGCCAAGATCTCTACTCCAGATTTGAGCTTAAGAATTTTCTTTG
CAAGAGCTCCTGCAGCCACCCTTCCAATGGTCTCTCTTGCTGATGATCTTCCACCTCCCTGCAC
TGCTCTAACACCGTATTTGAAGTCATAAGTTGCATCTGCATGTGAAGGTCTGTAGGCCTTAGCC
ATTTCACTGTAATCACCCCCTCTCTGATCTGTGTTCGGGACAAAAACATGAATTGGTGTCCCAG
TGGTCATTCCTTCATGTGTCCCTGAAAGAATTTTGCAAGTGTCAGTCTCCTTTCTTGGGGTGGT
TATTCTGCTCTGGCCTGGCCGTCTCCGGTCGAGTTCTACTTGCATATCTGCTTCAGTAAGTGGG
ATTCTGGGTGGGCATCCACTGATTACGCAACCAACACCGCCTCCATGAGACTCTCCATAAGTTG
CAACCTGGAAGTAGTTCCCAAATACATTTGCAGACGCCTTCACCT
						
					

						Genomic sequence
						
							CCCAATAGTCGCGGTTGGCTTGAAAGCTACTTTGAAGTATATAATTTCACCATTTGATATCCCT
CCCTGCATGGACATACACATTATAAATACTAAGACAACGGTGTTATACTATTTACTAGTATTTT
TTTAATCTAGAATGCAGCTAGATGTAGCGAAAACAATAAATAATATAACTGTAAAACAACAGGG
GACTAGGATAAAACCTGCACACCGCCTGAACGATTAGTTCTTGTTCTCACATTTCCAGCCTCAT
CCATATAGAACTCATCATTATGCTCACTTCCAGTGTAGTCAGTACCTGGGCACAAGTTATTAGT
TAGTATTGAGGCCAGTAGCAAAGTAGTTGGCATGCACAGCTATTTGCAAAAAATAAATCAACTA
ATTATGTGCAGATTAATTCAATTACCTGCAAATCCACTGCCGATCTCAAACCCCTTGCTTGCAG
GAAGAGAAAGCATAGCTTTCGCCAATTCAGCCTCAAGTTTGTCAAATACAGGAGAGCCAATCCC
CTACAAATTGTCGTTGCTCTATCATATCAGTAGCTCAACAGAGAAAAAATATTTTTCTAATGAA
TGATCAAGCTTCAATGTTCAAACAGATTTTGTGCAAATTCCAAAGCAGGGCGGTCATAAGCAGA
GAGTGCAAACGATGAGCTTATTTAGTTTATTTTAGCTGGAGGACATATATCAAAATAAACATAA
CTGCAGGGTTTAAATACTTACACGAGGAACATTTCTTGCAATGCATGTGACCACACCACCAATC
GAATCACCTCTAACTCGTACTTTATCGATTGCATCAATCATCTTCTGTGCATATTCTGGATCAG
GACATCTAACAATGTTGCTTTCTATCTGGAAAATAAAATAAATATAATATTCAGCAAATGCCTT
CAGAGACTGTCCAGAAGTGTTAAACTTGAAAATGGTGGGAACCACAGACCTGTTCCATTGTTAC
AGTGTCATAATCAACGGCATCTTCTGGTAGTACAACTTGATGCACCTTGGACACAAACGCCAAG
ATCTGCAATTGAAAATATTTTGCTGCTATGAGACATTGGACAGAATTTGTTGCATGGCTTCAGC
ATATTTAGGATGTATATTCGAATGCTGATTCATTTTTCTTCAGCGTCCAATTGTTCATGGATAA
GTAAACATGTCAATTGCGATGCTTGATACAAATCTTGATCAGTTAAGAGTAAAAGGTTGCCAAG
TAGGATCACACCTCTACTCCAGATTTGAGCTTAAGAATTTTCTTTGCAAGAGCTCCTGCAGCCA
CCCTTCCAATGGTCTCTCTTGCTGATGATCTTCCACCTCCCTATCATTCGGTTGATAGAAGATG
TTGGTTAGGCATTCAAGTAAGAGAAGTTTAGGTACCACAGTTTTAGACATTCAAATAAGAGAAG
TTTAGGTACTACAGTTGATGGTTCAATGAATGGCTATATTACACATTATAAAACATGAAAAAAA
GTACCTGCACTGCTCTAACACCGTATTTGAAGTCATAAGTTGCATCTGCATGTGAAGGTCTGTA
GGCCTTAGCCATTTCACTGTAATCCTGTGGGAATGAATATGGTTATTTCCAGCTTTAATATGTT
ACTCCCTCTGTCCCAAAATATAAGCATTTTTAGCTATGAATCTGGGCAATTGTGTGTCCAGATT
CGTAGCCAAAAGTTGTTATATTTTGGGACGGAGGTAGTATTTCAATACATTAATGCGAAAAAAC
TTAGGTACTTACACCCCCTCTCTGATCTGTGTTCGGGACAAAAACATGAATTGGTGTCCCAGTG
GTCATTCCTGCAGAAAACCCATCAATGCTATCGTGAATATGAGACAAGCTAATACTCTAAATTC
AAAAATATTTTTTCCAAGTTTATCCCATGTCCTGAAAGACACCATACCTTCATGTGTCCCTGAA
AGAATTTTGCAAGTGTCAGTCTCCTTTCTTGGGGTGGTTATTCTGCTCTGGCCTGGCCGTCTGT
GATAATCAAAATTTTATGTCAGTTAACATTATGGTGTAATGTAACATAACAATCATATTCTGCA
AGAGGTTAGCAGGATTGCATTTGCATAATCCTGTTTTTGACAGAAGCCAGCTGTCAGCACAGAA
ATAATGGATTTTTCAAGAGACAAACCTCCGGTCGAGTTCTACTTGCATATCTGCTTCAGTAAGT
GGGATTCTGGGTGGGCATCCACTGATTACGCAACCAACACCGCCTCCATGAGACTCTCCATAAG
TTGCAACCTGGAAGTAGTTCCCAAATACATTTGCAGACGCCTTCACCT
						
					

			
		

Sequences
Splice junction sequence	GGGCATCCACTGATTACGCAACCAACACCGCCTCCATGAGACTCTCCATAAGTTGCAACCTGGA AGTAGTTCCCAAATACATTTGCAGACGCCTTCACCTcccaatagtcgcggttggcttgaaagct actttgaagtatataatttcaccatttgatatccctccctgcatggacatacacattataaata ctaagaca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 941 Transcript exons: 8178732-8178797,8178938-8179032,8179141-8179243,8179457- 8179588,8179677-8179757,8179959-8180051,8180208-8180291, 8180472-8180530,8180635-8180711,8180869-8181019 CCCAATAGTCGCGGTTGGCTTGAAAGCTACTTTGAAGTATATAATTTCACCATTTGATATCCCT CCCTGCACACCGCCTGAACGATTAGTTCTTGTTCTCACATTTCCAGCCTCATCCATATAGAACT CATCATTATGCTCACTTCCAGTGTAGTCAGTACCTGCAAATCCACTGCCGATCTCAAACCCCTT GCTTGCAGGAAGAGAAAGCATAGCTTTCGCCAATTCAGCCTCAAGTTTGTCAAATACAGGAGAG CCAATCCCACGAGGAACATTTCTTGCAATGCATGTGACCACACCACCAATCGAATCACCTCTAA CTCGTACTTTATCGATTGCATCAATCATCTTCTGTGCATATTCTGGATCAGGACATCTAACAAT GTTGCTTTCTATCTGTTCCATTGTTACAGTGTCATAATCAACGGCATCTTCTGGTAGTACAACT TGATGCACCTTGGACACAAACGCCAAGATCTCTACTCCAGATTTGAGCTTAAGAATTTTCTTTG CAAGAGCTCCTGCAGCCACCCTTCCAATGGTCTCTCTTGCTGATGATCTTCCACCTCCCTGCAC TGCTCTAACACCGTATTTGAAGTCATAAGTTGCATCTGCATGTGAAGGTCTGTAGGCCTTAGCC ATTTCACTGTAATCACCCCCTCTCTGATCTGTGTTCGGGACAAAAACATGAATTGGTGTCCCAG TGGTCATTCCTTCATGTGTCCCTGAAAGAATTTTGCAAGTGTCAGTCTCCTTTCTTGGGGTGGT TATTCTGCTCTGGCCTGGCCGTCTCCGGTCGAGTTCTACTTGCATATCTGCTTCAGTAAGTGGG ATTCTGGGTGGGCATCCACTGATTACGCAACCAACACCGCCTCCATGAGACTCTCCATAAGTTG CAACCTGGAAGTAGTTCCCAAATACATTTGCAGACGCCTTCACCT
Genomic sequence	CCCAATAGTCGCGGTTGGCTTGAAAGCTACTTTGAAGTATATAATTTCACCATTTGATATCCCT CCCTGCATGGACATACACATTATAAATACTAAGACAACGGTGTTATACTATTTACTAGTATTTT TTTAATCTAGAATGCAGCTAGATGTAGCGAAAACAATAAATAATATAACTGTAAAACAACAGGG GACTAGGATAAAACCTGCACACCGCCTGAACGATTAGTTCTTGTTCTCACATTTCCAGCCTCAT CCATATAGAACTCATCATTATGCTCACTTCCAGTGTAGTCAGTACCTGGGCACAAGTTATTAGT TAGTATTGAGGCCAGTAGCAAAGTAGTTGGCATGCACAGCTATTTGCAAAAAATAAATCAACTA ATTATGTGCAGATTAATTCAATTACCTGCAAATCCACTGCCGATCTCAAACCCCTTGCTTGCAG GAAGAGAAAGCATAGCTTTCGCCAATTCAGCCTCAAGTTTGTCAAATACAGGAGAGCCAATCCC CTACAAATTGTCGTTGCTCTATCATATCAGTAGCTCAACAGAGAAAAAATATTTTTCTAATGAA TGATCAAGCTTCAATGTTCAAACAGATTTTGTGCAAATTCCAAAGCAGGGCGGTCATAAGCAGA GAGTGCAAACGATGAGCTTATTTAGTTTATTTTAGCTGGAGGACATATATCAAAATAAACATAA CTGCAGGGTTTAAATACTTACACGAGGAACATTTCTTGCAATGCATGTGACCACACCACCAATC GAATCACCTCTAACTCGTACTTTATCGATTGCATCAATCATCTTCTGTGCATATTCTGGATCAG GACATCTAACAATGTTGCTTTCTATCTGGAAAATAAAATAAATATAATATTCAGCAAATGCCTT CAGAGACTGTCCAGAAGTGTTAAACTTGAAAATGGTGGGAACCACAGACCTGTTCCATTGTTAC AGTGTCATAATCAACGGCATCTTCTGGTAGTACAACTTGATGCACCTTGGACACAAACGCCAAG ATCTGCAATTGAAAATATTTTGCTGCTATGAGACATTGGACAGAATTTGTTGCATGGCTTCAGC ATATTTAGGATGTATATTCGAATGCTGATTCATTTTTCTTCAGCGTCCAATTGTTCATGGATAA GTAAACATGTCAATTGCGATGCTTGATACAAATCTTGATCAGTTAAGAGTAAAAGGTTGCCAAG TAGGATCACACCTCTACTCCAGATTTGAGCTTAAGAATTTTCTTTGCAAGAGCTCCTGCAGCCA CCCTTCCAATGGTCTCTCTTGCTGATGATCTTCCACCTCCCTATCATTCGGTTGATAGAAGATG TTGGTTAGGCATTCAAGTAAGAGAAGTTTAGGTACCACAGTTTTAGACATTCAAATAAGAGAAG TTTAGGTACTACAGTTGATGGTTCAATGAATGGCTATATTACACATTATAAAACATGAAAAAAA GTACCTGCACTGCTCTAACACCGTATTTGAAGTCATAAGTTGCATCTGCATGTGAAGGTCTGTA GGCCTTAGCCATTTCACTGTAATCCTGTGGGAATGAATATGGTTATTTCCAGCTTTAATATGTT ACTCCCTCTGTCCCAAAATATAAGCATTTTTAGCTATGAATCTGGGCAATTGTGTGTCCAGATT CGTAGCCAAAAGTTGTTATATTTTGGGACGGAGGTAGTATTTCAATACATTAATGCGAAAAAAC TTAGGTACTTACACCCCCTCTCTGATCTGTGTTCGGGACAAAAACATGAATTGGTGTCCCAGTG GTCATTCCTGCAGAAAACCCATCAATGCTATCGTGAATATGAGACAAGCTAATACTCTAAATTC AAAAATATTTTTTCCAAGTTTATCCCATGTCCTGAAAGACACCATACCTTCATGTGTCCCTGAA AGAATTTTGCAAGTGTCAGTCTCCTTTCTTGGGGTGGTTATTCTGCTCTGGCCTGGCCGTCTGT GATAATCAAAATTTTATGTCAGTTAACATTATGGTGTAATGTAACATAACAATCATATTCTGCA AGAGGTTAGCAGGATTGCATTTGCATAATCCTGTTTTTGACAGAAGCCAGCTGTCAGCACAGAA ATAATGGATTTTTCAAGAGACAAACCTCCGGTCGAGTTCTACTTGCATATCTGCTTCAGTAAGT GGGATTCTGGGTGGGCATCCACTGATTACGCAACCAACACCGCCTCCATGAGACTCTCCATAAG TTGCAACCTGGAAGTAGTTCCCAAATACATTTGCAGACGCCTTCACCT

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_014145 						
					
						PMCS
						0.340464033

Conservation Information
Conserved circRNAs	osi_circ_014145
PMCS	0.340464033

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							8180956-8178992(+)
8180893-8181016(-)
						
						Potential amino acid sequence
						
							MRLSISCNLEVVPKYICRRLHLPIVAVGLKATLKYIISPFDIPPCTPPERLVLVLTFPASSI*(
+)
MQVELDRRRPGQSRITTPRKETDTCKILSGTHEGMTTGTPIHVFVPNTDQRGGDYSEMAKAYRP
SHADATYDFKYGVRAVQGGGRSSARETIGRVAAGALAKKILKLKSGVEILAFVSKVHQVVLPED
AVDYDTVTMEQIESNIVRCPDPEYAQKMIDAIDKVRVRGDSIGGVVTCIARNVPRGIGSPVFDK
LEAELAKAMLSLPASKGFEIGSGFAGTDYTGSEHNDEFYMDEAGNVRTRTNRSGGVQGGISNGE
IIYFKVAFKPTATIGR*(-)
						
						Sponge-miRNAs
						
							osa-miR156j-5p osa-miR156k 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	8180956-8178992(+) 8180893-8181016(-)
Potential amino acid sequence	MRLSISCNLEVVPKYICRRLHLPIVAVGLKATLKYIISPFDIPPCTPPERLVLVLTFPASSI( +) MQVELDRRRPGQSRITTPRKETDTCKILSGTHEGMTTGTPIHVFVPNTDQRGGDYSEMAKAYRP SHADATYDFKYGVRAVQGGGRSSARETIGRVAAGALAKKILKLKSGVEILAFVSKVHQVVLPED AVDYDTVTMEQIESNIVRCPDPEYAQKMIDAIDKVRVRGDSIGGVVTCIARNVPRGIGSPVFDK LEAELAKAMLSLPASKGFEIGSGFAGTDYTGSEHNDEFYMDEAGNVRTRTNRSGGVQGGISNGE IIYFKVAFKPTATIGR(-)
Sponge-miRNAs	osa-miR156j-5p osa-miR156k
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os03g0254800_circ_g.4

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