Plant Circular RNA Database

Detail information of AT2G21160_circ_g.5

			
						 General Information
					
						CircRNA Name
						AT2G21160_circ_g.5
					
						ID in PlantcircBase
						ath_circ_014141
					
						Alias
						Ath_circ_FC1281
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr2: 9068944-9069914  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, PcircRNA_finder
					
						Parent gene
						
							AT2G21160						
					
						Parent gene annotation
						
							Translocon-associated protein subunit alpha
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT2G21160_circ_g.1 AT2G21160_circ_g.2 AT2G21160_circ_g.3 AT2G21160_circ_g.4 AT2G21160_circ_g.6 AT2G21160_circ_g.7 AT2G21160_circ_g.8 						
					
						Support reads
						21
					
						Tissues
						root, aerial, whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT2G21160.1:5
AT2G21160.2:5

General Information
CircRNA Name	AT2G21160_circ_g.5
ID in PlantcircBase	ath_circ_014141
Alias	Ath_circ_FC1281
Organism	Arabidpsis thaliana
Position	chr2: 9068944-9069914 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, PcircRNA_finder
Parent gene	AT2G21160
Parent gene annotation	Translocon-associated protein subunit alpha
Parent gene strand	+
Alternative splicing	AT2G21160_circ_g.1 AT2G21160_circ_g.2 AT2G21160_circ_g.3 AT2G21160_circ_g.4 AT2G21160_circ_g.6 AT2G21160_circ_g.7 AT2G21160_circ_g.8
Support reads	21
Tissues	root, aerial, whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT2G21160.1:5 AT2G21160.2:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TCTTCTCAGCGGCGAATCTGTTTTCCTTTTAACACTCGGAATCGGTCTCCTCCTCCTCCTCGGT
CTATGGGCATACAGCCAAGTTCAGCGTCTCACCAAGttgctagatgtcaatcggatgccgagga
tcattcaagtcttgttgacgatgttgtgggagaaaacactgatgatgcggttgaggaagatgac
catgattt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 590
Transcript exons: 9068944-9069111,9069202-9069252,9069349-9069437,9069526-
9069603,9069711-9069914
TTGCTAGATGTCAATCGGATGCCGAGGATCATTCAAGTCTTGTTGACGATGTTGTGGGAGAAAA
CACTGATGATGCGGTTGAGGAAGATGACCATGATTTGGATATGAACTTGTCCTCATTTCCAGGA
GTTGAGACTGTTTGTGTTTTCCCCAAAAACAGTGCCAAATTGGTTCCAGCTGGAGAAGAGACTG
AGCTCCTTGTTGGTCTTAAAAATGAAGGGAAAACTAGAGTAGGTGTGATGGGAATTAGGGCCAG
CGTTCATCTTCCTTATGATCATAAGTTGTTGGTTCAGAATCTAACCATGTTGAGACTTAACAAT
GCATCCATCCCAACGTCTCTTCAAGCAACATTCCCATACATCTTTGCAGTCAGCCAGTACTTGC
AGCCTGGAGCATTTGATCTGGTGGGTTATATCATATACGATGTGGAAGGAAAGCCATACCAGAG
TGTCTTCTACAATGGAACCATAGAGGTAGTTGAATCTGGAGGTCTTCTCAGCGGCGAATCTGTT
TTCCTTTTAACACTCGGAATCGGTCTCCTCCTCCTCCTCGGTCTATGGGCATACAGCCAAGTTC
AGCGTCTCACCAAG
						
					

						Genomic sequence
						
							TTGCTAGATGTCAATCGGATGCCGAGGATCATTCAAGTCTTGTTGACGATGTTGTGGGAGAAAA
CACTGATGATGCGGTTGAGGAAGATGACCATGATTTGGATATGAACTTGTCCTCATTTCCAGGA
GTTGAGACTGTTTGTGTTTTCCCCAAAAACAGTGCCAAATGTATGTGTTTACTCCTCCTTTCTT
ACGTATTTATCTATCCGTTGTTGTGATCTTAAAGATTTGGTTTTTAATGCTCTTTGTTTCCTGC
AGTGGTTCCAGCTGGAGAAGAGACTGAGCTCCTTGTTGGTCTTAAAAATGAAGGTAGCTAATAC
CTCTTTTAGCTTTAGCTCTCTTTGTGTTGAGTTTTCTGTTTTGTTGTCAAAACTAAATATCTCC
AAATTGGCTGTGACTTAACAGGGAAAACTAGAGTAGGTGTGATGGGAATTAGGGCCAGCGTTCA
TCTTCCTTATGATCATAAGTTGTTGGTTCAGAATCTAACCATGTTGGTAAGCATTTGACTAGGA
AACTATTTGTCTAAATATTTGTTATCATTTTATTGTTTTTTGTTGTTCACATGCTTGCATTCTA
TTGCAGAGACTTAACAATGCATCCATCCCAACGTCTCTTCAAGCAACATTCCCATACATCTTTG
CAGTCAGCCAGTACTTGCAGGTAAACATATTCCACAATAGACAATACATCATTCTCGTTCATTC
TTAAAATACAATTGTTATTGAGAAAAAACTCCGAAAGATCTAATCTTGCTTGGTGTTGCACAGC
CTGGAGCATTTGATCTGGTGGGTTATATCATATACGATGTGGAAGGAAAGCCATACCAGAGTGT
CTTCTACAATGGAACCATAGAGGTAGTTGAATCTGGAGGTCTTCTCAGCGGCGAATCTGTTTTC
CTTTTAACACTCGGAATCGGTCTCCTCCTCCTCCTCGGTCTATGGGCATACAGCCAAGTTCAGC
GTCTCACCAAG
						
					

			
		

Sequences
Splice junction sequence	TCTTCTCAGCGGCGAATCTGTTTTCCTTTTAACACTCGGAATCGGTCTCCTCCTCCTCCTCGGT CTATGGGCATACAGCCAAGTTCAGCGTCTCACCAAGttgctagatgtcaatcggatgccgagga tcattcaagtcttgttgacgatgttgtgggagaaaacactgatgatgcggttgaggaagatgac catgattt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 590 Transcript exons: 9068944-9069111,9069202-9069252,9069349-9069437,9069526- 9069603,9069711-9069914 TTGCTAGATGTCAATCGGATGCCGAGGATCATTCAAGTCTTGTTGACGATGTTGTGGGAGAAAA CACTGATGATGCGGTTGAGGAAGATGACCATGATTTGGATATGAACTTGTCCTCATTTCCAGGA GTTGAGACTGTTTGTGTTTTCCCCAAAAACAGTGCCAAATTGGTTCCAGCTGGAGAAGAGACTG AGCTCCTTGTTGGTCTTAAAAATGAAGGGAAAACTAGAGTAGGTGTGATGGGAATTAGGGCCAG CGTTCATCTTCCTTATGATCATAAGTTGTTGGTTCAGAATCTAACCATGTTGAGACTTAACAAT GCATCCATCCCAACGTCTCTTCAAGCAACATTCCCATACATCTTTGCAGTCAGCCAGTACTTGC AGCCTGGAGCATTTGATCTGGTGGGTTATATCATATACGATGTGGAAGGAAAGCCATACCAGAG TGTCTTCTACAATGGAACCATAGAGGTAGTTGAATCTGGAGGTCTTCTCAGCGGCGAATCTGTT TTCCTTTTAACACTCGGAATCGGTCTCCTCCTCCTCCTCGGTCTATGGGCATACAGCCAAGTTC AGCGTCTCACCAAG
Genomic sequence	TTGCTAGATGTCAATCGGATGCCGAGGATCATTCAAGTCTTGTTGACGATGTTGTGGGAGAAAA CACTGATGATGCGGTTGAGGAAGATGACCATGATTTGGATATGAACTTGTCCTCATTTCCAGGA GTTGAGACTGTTTGTGTTTTCCCCAAAAACAGTGCCAAATGTATGTGTTTACTCCTCCTTTCTT ACGTATTTATCTATCCGTTGTTGTGATCTTAAAGATTTGGTTTTTAATGCTCTTTGTTTCCTGC AGTGGTTCCAGCTGGAGAAGAGACTGAGCTCCTTGTTGGTCTTAAAAATGAAGGTAGCTAATAC CTCTTTTAGCTTTAGCTCTCTTTGTGTTGAGTTTTCTGTTTTGTTGTCAAAACTAAATATCTCC AAATTGGCTGTGACTTAACAGGGAAAACTAGAGTAGGTGTGATGGGAATTAGGGCCAGCGTTCA TCTTCCTTATGATCATAAGTTGTTGGTTCAGAATCTAACCATGTTGGTAAGCATTTGACTAGGA AACTATTTGTCTAAATATTTGTTATCATTTTATTGTTTTTTGTTGTTCACATGCTTGCATTCTA TTGCAGAGACTTAACAATGCATCCATCCCAACGTCTCTTCAAGCAACATTCCCATACATCTTTG CAGTCAGCCAGTACTTGCAGGTAAACATATTCCACAATAGACAATACATCATTCTCGTTCATTC TTAAAATACAATTGTTATTGAGAAAAAACTCCGAAAGATCTAATCTTGCTTGGTGTTGCACAGC CTGGAGCATTTGATCTGGTGGGTTATATCATATACGATGTGGAAGGAAAGCCATACCAGAGTGT CTTCTACAATGGAACCATAGAGGTAGTTGAATCTGGAGGTCTTCTCAGCGGCGAATCTGTTTTC CTTTTAACACTCGGAATCGGTCTCCTCCTCCTCCTCGGTCTATGGGCATACAGCCAAGTTCAGC GTCTCACCAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.327320591

Conservation Information
Conserved circRNAs	NA
PMCS	0.327320591

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017;Chen et al., 2017a

circRNA

Detail information of AT2G21160_circ_g.5

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