Plant Circular RNA Database

Detail information of Os03g0784400_circ_g.6

			
						 General Information
					
						CircRNA Name
						Os03g0784400_circ_g.6
					
						ID in PlantcircBase
						osa_circ_022138
					
						Alias
						Os03circ28554
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 32557681-32558951  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, CIRI, PcircRNA_finder, SMALT, Segemehl, circRNA_finder, find_circ
					
						Parent gene
						
							Os03g0784400						
					
						Parent gene annotation
						
							Protein of unknown function DUF1692 domain containing protein. (
Os03t0784400-01);Similar to predicted protein. (Os03t0784400-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os03g0784400_circ_g.1 Os03g0784400_circ_g.2 Os03g0784400_circ_g.3 Os03g0784400_circ_g.4 Os03g0784400_circ_g.5 Os03g0784400_circ_g.7 						
					
						Support reads
						2/9
					
						Tissues
						leaf/shoot, root, seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0784400-01:3

General Information
CircRNA Name	Os03g0784400_circ_g.6
ID in PlantcircBase	osa_circ_022138
Alias	Os03circ28554
Organism	Oryza sativa
Position	chr3: 32557681-32558951 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, CIRI, PcircRNA_finder, SMALT, Segemehl, circRNA_finder, find_circ
Parent gene	Os03g0784400
Parent gene annotation	Protein of unknown function DUF1692 domain containing protein. ( Os03t0784400-01);Similar to predicted protein. (Os03t0784400-02)
Parent gene strand	+
Alternative splicing	Os03g0784400_circ_g.1 Os03g0784400_circ_g.2 Os03g0784400_circ_g.3 Os03g0784400_circ_g.4 Os03g0784400_circ_g.5 Os03g0784400_circ_g.7
Support reads	2/9
Tissues	leaf/shoot, root, seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0784400-01:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TCTATCCTTTGGTCCAAAATATCCTGGAATTCATAATCCACTGGATGAGACTACAAGGATACTC
CATGATACAAGTGGGACCTTCAAATACTATATCAAGatcatgaccatgagcatgaagatgaaca
aaagaagcaagagcacacctttaatgaagatgcagaaaaaatggttaagagtgtgaaacaagca
atggaaaa

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 347
Transcript exons: 32557681-32557796,32557908-32557991,32558805-32558951
ATCATGACCATGAGCATGAAGATGAACAAAAGAAGCAAGAGCACACCTTTAATGAAGATGCAGA
AAAAATGGTTAAGAGTGTGAAACAAGCAATGGAAAATGGTGAAGGGTGCCGAGTTTATGGTGTT
CTAGATGTGCAGAGGGTTGCTGGTAACTTCCATATCTCGGTTCATGGTTTGAACATTTTTGTTG
CTGAGAAGATTTTTGATGGGTCAAGCCATGTGAACGTCAGTCATATCATCCATGATCTATCCTT
TGGTCCAAAATATCCTGGAATTCATAATCCACTGGATGAGACTACAAGGATACTCCATGATACA
AGTGGGACCTTCAAATACTATATCAAG
						
					

						Genomic sequence
						
							ATCATGACCATGAGCATGAAGATGAACAAAAGAAGCAAGAGCACACCTTTAATGAAGATGCAGA
AAAAATGGTTAAGAGTGTGAAACAAGCAATGGAAAATGGTGAAGGGTGCCGAGTAAGATATTTC
TCTACCAGTATCCTTTAATTTATGGAATGGTATTATTAAGGCTATTATCATCAGGCATACCATG
TACTTGTTAAAACATTTTGCTTTCAATTTTCACAGGTTTATGGTGTTCTAGATGTGCAGAGGGT
TGCTGGTAACTTCCATATCTCGGTTCATGGTTTGAACATTTTTGTTGCTGAGAAGGTTATTCAT
CTAAAACTGCATATTTTTTTTACTTCTGTGCTTCCATCTGTATGCTTTTTGCTGTGAAATATGT
TAATTTCTTTTTGTATGAAGTTGCCAGGATTATTGTATAAGCTTCTAGGTTTTCAGTTTATGAC
AGATCATGTTCACAAGGGTTTAAAATTATTCAACTAAAAATCCCTTCAGAAACTATTGAAACCA
GCCAGCTCCTACTTCCTGGTAATGCCATATTGGACAAGTGCTTTGTGCATCTTGTACAAGACTT
CCTAAAACTGTCCACAATTAGGATAATGTTTTAATGGTAAACAGATGCTAGTGATTACGAGAGA
AGGAAAATAACACAACCTAATGATAATAATAATAATAAGTTAGCCAGTAAACAACTCAATACTC
ACCAGTTTGGTGCACACCAGATCAAGCTGGGATTGGAACAAAAAAAAGGACGTGGGAAGTATCT
TCTTTTGGCCATCACATTATATAATTGTCAGCTTATATGAACCACAATATTTTCTTTACATTGG
ATGTGCTTATGTCTCTTCGATTTTTATGAAAAATATGCATTAAAAAGCAACCACCTAGGTAGCT
AGGCGGTGCACTACTGCCTAAGGCGCCTAATTGATTTATTATGCCGCATAGGTGGCTTGCTAGC
CCCGTCTTATGGTGTACTACACTCTGGTATGCCGCCTAACTAGCACATAAAGGCCTCAGTGATT
TCAACCTCTTTTTGTGCTACCTATAAATAAGTGTTTTATGCTTTTATTTGTATTGTTATTACTC
TCTATGTAAATTTTCATTTTCATCTGTTGTATGTAGATTTTTGATGGGTCAAGCCATGTGAACG
TCAGTCATATCATCCATGATCTATCCTTTGGTCCAAAATATCCTGGAATTCATAATCCACTGGA
TGAGACTACAAGGATACTCCATGATACAAGTGGGACCTTCAAATACTATATCAAG
						
					

			
		

Sequences
Splice junction sequence	TCTATCCTTTGGTCCAAAATATCCTGGAATTCATAATCCACTGGATGAGACTACAAGGATACTC CATGATACAAGTGGGACCTTCAAATACTATATCAAGatcatgaccatgagcatgaagatgaaca aaagaagcaagagcacacctttaatgaagatgcagaaaaaatggttaagagtgtgaaacaagca atggaaaa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 347 Transcript exons: 32557681-32557796,32557908-32557991,32558805-32558951 ATCATGACCATGAGCATGAAGATGAACAAAAGAAGCAAGAGCACACCTTTAATGAAGATGCAGA AAAAATGGTTAAGAGTGTGAAACAAGCAATGGAAAATGGTGAAGGGTGCCGAGTTTATGGTGTT CTAGATGTGCAGAGGGTTGCTGGTAACTTCCATATCTCGGTTCATGGTTTGAACATTTTTGTTG CTGAGAAGATTTTTGATGGGTCAAGCCATGTGAACGTCAGTCATATCATCCATGATCTATCCTT TGGTCCAAAATATCCTGGAATTCATAATCCACTGGATGAGACTACAAGGATACTCCATGATACA AGTGGGACCTTCAAATACTATATCAAG
Genomic sequence	ATCATGACCATGAGCATGAAGATGAACAAAAGAAGCAAGAGCACACCTTTAATGAAGATGCAGA AAAAATGGTTAAGAGTGTGAAACAAGCAATGGAAAATGGTGAAGGGTGCCGAGTAAGATATTTC TCTACCAGTATCCTTTAATTTATGGAATGGTATTATTAAGGCTATTATCATCAGGCATACCATG TACTTGTTAAAACATTTTGCTTTCAATTTTCACAGGTTTATGGTGTTCTAGATGTGCAGAGGGT TGCTGGTAACTTCCATATCTCGGTTCATGGTTTGAACATTTTTGTTGCTGAGAAGGTTATTCAT CTAAAACTGCATATTTTTTTTACTTCTGTGCTTCCATCTGTATGCTTTTTGCTGTGAAATATGT TAATTTCTTTTTGTATGAAGTTGCCAGGATTATTGTATAAGCTTCTAGGTTTTCAGTTTATGAC AGATCATGTTCACAAGGGTTTAAAATTATTCAACTAAAAATCCCTTCAGAAACTATTGAAACCA GCCAGCTCCTACTTCCTGGTAATGCCATATTGGACAAGTGCTTTGTGCATCTTGTACAAGACTT CCTAAAACTGTCCACAATTAGGATAATGTTTTAATGGTAAACAGATGCTAGTGATTACGAGAGA AGGAAAATAACACAACCTAATGATAATAATAATAATAAGTTAGCCAGTAAACAACTCAATACTC ACCAGTTTGGTGCACACCAGATCAAGCTGGGATTGGAACAAAAAAAAGGACGTGGGAAGTATCT TCTTTTGGCCATCACATTATATAATTGTCAGCTTATATGAACCACAATATTTTCTTTACATTGG ATGTGCTTATGTCTCTTCGATTTTTATGAAAAATATGCATTAAAAAGCAACCACCTAGGTAGCT AGGCGGTGCACTACTGCCTAAGGCGCCTAATTGATTTATTATGCCGCATAGGTGGCTTGCTAGC CCCGTCTTATGGTGTACTACACTCTGGTATGCCGCCTAACTAGCACATAAAGGCCTCAGTGATT TCAACCTCTTTTTGTGCTACCTATAAATAAGTGTTTTATGCTTTTATTTGTATTGTTATTACTC TCTATGTAAATTTTCATTTTCATCTGTTGTATGTAGATTTTTGATGGGTCAAGCCATGTGAACG TCAGTCATATCATCCATGATCTATCCTTTGGTCCAAAATATCCTGGAATTCATAATCCACTGGA TGAGACTACAAGGATACTCCATGATACAAGTGGGACCTTCAAATACTATATCAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_005157* osi_circ_013724 						
					
						PMCS
						0.478678807

Conservation Information
Conserved circRNAs	osi_circ_005157* osi_circ_013724
PMCS	0.478678807

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							32558849-32557684(+)
32557750-32557761(+)
						
						Potential amino acid sequence
						
							MIYPLVQNILEFIIHWMRLQGYSMIQVGPSNTISRS*(+)
MVKSVKQAMENGEGCRVYGVLDVQRVAGNFHISVHGLNIFVAEKIFDGSSHVNVSHIIHDLSFG
PKYPGIHNPLDETTRILHDTSGTFKYYIKIMTMSMKMNKRSKSTPLMKMQKKWLRV*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	32558849-32557684(+) 32557750-32557761(+)
Potential amino acid sequence	MIYPLVQNILEFIIHWMRLQGYSMIQVGPSNTISRS(+) MVKSVKQAMENGEGCRVYGVLDVQRVAGNFHISVHGLNIFVAEKIFDGSSHVNVSHIIHDLSFG PKYPGIHNPLDETTRILHDTSGTFKYYIKIMTMSMKMNKRSKSTPLMKMQKKWLRV(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015;Chu et al., 2017

circRNA

Detail information of Os03g0784400_circ_g.6

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