Plant Circular RNA Database

Detail information of Os06g0114000_circ_g.32

			
						 General Information
					
						CircRNA Name
						Os06g0114000_circ_g.32
					
						ID in PlantcircBase
						osa_circ_029426
					
						Alias
						Os_ciR10827
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr6: 815013-815458  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os06g0114066						
					
						Parent gene annotation
						
							Hypothetical protein. (Os06t0114066-00)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os06g0114000_circ_g.1 Os06g0114000_circ_g.2 Os06g0114000_circ_g.3 Os06g0114000_circ_g.4 Os06g0114000_circ_g.5 Os06g0114000_circ_g.6 Os06g0114000_circ_g.7 Os06g0114000_circ_g.8 Os06g0114000_circ_g.9 Os06g0114000_circ_g.10 Os06g0114000_circ_g.11 Os06g0114000_circ_g.12 Os06g0114000_circ_g.13 Os06g0114000_circ_g.14 Os06g0114000_circ_g.15 Os06g0114000_circ_g.16 Os06g0114000_circ_g.17 Os06g0114000_circ_g.18 Os06g0114000_circ_g.19 Os06g0114000_circ_g.20 Os06g0114000_circ_g.21 Os06g0114000_circ_g.22 Os06g0114000_circ_g.23 Os06g0114000_circ_g.24 Os06g0114000_circ_g.25 Os06g0114000_circ_g.26 Os06g0114000_circ_g.27 Os06g0114000_circ_g.28 Os06g0114000_circ_g.29 Os06g0114000_circ_g.30 Os06g0114000_circ_g.31 						
					
						Support reads
						2/2
					
						Tissues
						root/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os06t0114000-03:1
Os06t0114000-02:2
Os06t0114066-00:2
Os06t0114000-03:1
Os06t0114000-02:2

General Information
CircRNA Name	Os06g0114000_circ_g.32
ID in PlantcircBase	osa_circ_029426
Alias	Os_ciR10827
Organism	Oryza sativa
Position	chr6: 815013-815458 JBrowse»
Reference genome	IRGSP-1.0.38
Type	u-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os06g0114066
Parent gene annotation	Hypothetical protein. (Os06t0114066-00)
Parent gene strand	+
Alternative splicing	Os06g0114000_circ_g.1 Os06g0114000_circ_g.2 Os06g0114000_circ_g.3 Os06g0114000_circ_g.4 Os06g0114000_circ_g.5 Os06g0114000_circ_g.6 Os06g0114000_circ_g.7 Os06g0114000_circ_g.8 Os06g0114000_circ_g.9 Os06g0114000_circ_g.10 Os06g0114000_circ_g.11 Os06g0114000_circ_g.12 Os06g0114000_circ_g.13 Os06g0114000_circ_g.14 Os06g0114000_circ_g.15 Os06g0114000_circ_g.16 Os06g0114000_circ_g.17 Os06g0114000_circ_g.18 Os06g0114000_circ_g.19 Os06g0114000_circ_g.20 Os06g0114000_circ_g.21 Os06g0114000_circ_g.22 Os06g0114000_circ_g.23 Os06g0114000_circ_g.24 Os06g0114000_circ_g.25 Os06g0114000_circ_g.26 Os06g0114000_circ_g.27 Os06g0114000_circ_g.28 Os06g0114000_circ_g.29 Os06g0114000_circ_g.30 Os06g0114000_circ_g.31
Support reads	2/2
Tissues	root/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os06t0114000-03:1 Os06t0114000-02:2 Os06t0114066-00:2 Os06t0114000-03:1 Os06t0114000-02:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CCGAAGATAGAGAAGATGGCTTCTTGTCTGATACAATTCCGGTTGGCGCAGCCATGAGGCCAAC
CGTAGAAGTGGCACCGAATGTTGAAGCCATGATTTCctctcttgcaactgtaacaccatcattg
acaatcctaggggacccatacttgctctccaaaacaacattccttccctttggtcccagtgtaa
ctccaaca

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 351
Transcript exons: 815013-815138,815234-815458
CTCTCTTGCAACTGTAACACCATCATTGACAATCCTAGGGGACCCATACTTGCTCTCCAAAACA
ACATTCCTTCCCTTTGGTCCCAGTGTAACTCCAACAAGGTCAGCAAGCTTGTTTACTCCAGTCT
GAAGCTTCTTGATAGCCGAGCCATCCTTGTTGAAGTACAACTCCTTGGCAGCTTTTACCCTGAA
GTTGCACTTCCTCTGAAGGCGTGCATTGCGTGGTCGGCTGGCAACAGAAACAGATGACACCGAA
GATAGAGAAGATGGCTTCTTGTCTGATACAATTCCGGTTGGCGCAGCCATGAGGCCAACCGTAG
AAGTGGCACCGAATGTTGAAGCCATGATTTC
						
						Genomic sequence
						
							CTCTCTTGCAACTGTAACACCATCATTGACAATCCTAGGGGACCCATACTTGCTCTCCAAAACA
ACATTCCTTCCCTTTGGTCCCAGTGTAACTCCAACAAGGTCAGCAAGCTTGTTTACTCCAGTCT
GACATGTAGAAATAACTAATGAGTGCCTGGTCAAGGCTAAGAATCATAGTGACCAAAATTTGAT
AAGATGTGTCCAAGAACTTTGGAGCTCACCTGAAGCTTCTTGATAGCCGAGCCATCCTTGTTGA
AGTACAACTCCTTGGCAGCTTTTACCCTGAAGTTGCACTTCCTCTGAAGGCGTGCATTGCGTGG
TCGGCTGGCAACAGAAACAGATGACACCGAAGATAGAGAAGATGGCTTCTTGTCTGATACAATT
CCGGTTGGCGCAGCCATGAGGCCAACCGTAGAAGTGGCACCGAATGTTGAAGCCATGATTTC

Sequences
Splice junction sequence	CCGAAGATAGAGAAGATGGCTTCTTGTCTGATACAATTCCGGTTGGCGCAGCCATGAGGCCAAC CGTAGAAGTGGCACCGAATGTTGAAGCCATGATTTCctctcttgcaactgtaacaccatcattg acaatcctaggggacccatacttgctctccaaaacaacattccttccctttggtcccagtgtaa ctccaaca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 351 Transcript exons: 815013-815138,815234-815458 CTCTCTTGCAACTGTAACACCATCATTGACAATCCTAGGGGACCCATACTTGCTCTCCAAAACA ACATTCCTTCCCTTTGGTCCCAGTGTAACTCCAACAAGGTCAGCAAGCTTGTTTACTCCAGTCT GAAGCTTCTTGATAGCCGAGCCATCCTTGTTGAAGTACAACTCCTTGGCAGCTTTTACCCTGAA GTTGCACTTCCTCTGAAGGCGTGCATTGCGTGGTCGGCTGGCAACAGAAACAGATGACACCGAA GATAGAGAAGATGGCTTCTTGTCTGATACAATTCCGGTTGGCGCAGCCATGAGGCCAACCGTAG AAGTGGCACCGAATGTTGAAGCCATGATTTC
Genomic sequence	CTCTCTTGCAACTGTAACACCATCATTGACAATCCTAGGGGACCCATACTTGCTCTCCAAAACA ACATTCCTTCCCTTTGGTCCCAGTGTAACTCCAACAAGGTCAGCAAGCTTGTTTACTCCAGTCT GACATGTAGAAATAACTAATGAGTGCCTGGTCAAGGCTAAGAATCATAGTGACCAAAATTTGAT AAGATGTGTCCAAGAACTTTGGAGCTCACCTGAAGCTTCTTGATAGCCGAGCCATCCTTGTTGA AGTACAACTCCTTGGCAGCTTTTACCCTGAAGTTGCACTTCCTCTGAAGGCGTGCATTGCGTGG TCGGCTGGCAACAGAAACAGATGACACCGAAGATAGAGAAGATGGCTTCTTGTCTGATACAATT CCGGTTGGCGCAGCCATGAGGCCAACCGTAGAAGTGGCACCGAATGTTGAAGCCATGATTTC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_016932 						
					
						PMCS
						0.249867545

Conservation Information
Conserved circRNAs	osi_circ_016932
PMCS	0.249867545

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							815413-815234(+)
815454-815015(-)
815082-815292(-)
						
						Potential amino acid sequence
						
							MRPTVEVAPNVEAMISSLATVTPSLTILGDPYLLSKTTFLPFGPSVTPTRSASLFTPV*(+)
MASTFGATSTVGLMAAPTGIVSDKKPSSLSSVSSVSVASRPRNARLQRKCNFRVKAAKELYFNK
DGSAIKKLQTGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREEIMASTFGATSTV
GLMAAPTGIVSDKKPSSLSSVSSVSVASRPRNARLQRKCNFRVKAAKELYFNKDGSAIKKLQTG
VNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREEIMASTFGATSTVGLMAAPTGIVS
DKKPSSLSSVSSVSVASRPRNARLQRKCNFRVKAAKELYFNKDGSAIKKLQTGVNKLADLVGVT
LGPKGRNVVLESKYGSPRIVNDGVTVAREEIMASTFGATSTVGLMAAPTGIVSDKKPSSLSSVS
SVSVASRPRNARLQRKCNFRVKAAKELYFNKDGSAIKKLQTGVNKLADLVGVTLGPKGRNVVLE
SKYGSPRIVNDGVTVARE(-)
MLFWRASMGPLGLSMMVLQLQERKSWLQHSVPLLRLASWLRQPELYQTRSHLLYLRCHLFLLPA
DHAMHAFRGSATSG*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	815413-815234(+) 815454-815015(-) 815082-815292(-)
Potential amino acid sequence	MRPTVEVAPNVEAMISSLATVTPSLTILGDPYLLSKTTFLPFGPSVTPTRSASLFTPV(+) MASTFGATSTVGLMAAPTGIVSDKKPSSLSSVSSVSVASRPRNARLQRKCNFRVKAAKELYFNK DGSAIKKLQTGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREEIMASTFGATSTV GLMAAPTGIVSDKKPSSLSSVSSVSVASRPRNARLQRKCNFRVKAAKELYFNKDGSAIKKLQTG VNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREEIMASTFGATSTVGLMAAPTGIVS DKKPSSLSSVSSVSVASRPRNARLQRKCNFRVKAAKELYFNKDGSAIKKLQTGVNKLADLVGVT LGPKGRNVVLESKYGSPRIVNDGVTVAREEIMASTFGATSTVGLMAAPTGIVSDKKPSSLSSVS SVSVASRPRNARLQRKCNFRVKAAKELYFNKDGSAIKKLQTGVNKLADLVGVTLGPKGRNVVLE SKYGSPRIVNDGVTVARE(-) MLFWRASMGPLGLSMMVLQLQERKSWLQHSVPLLRLASWLRQPELYQTRSHLLYLRCHLFLLPA DHAMHAFRGSATSG(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os06g0114000_circ_g.32

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