Plant Circular RNA Database

Detail information of Os06g0245800_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os06g0245800_circ_g.1
					
						ID in PlantcircBase
						osa_circ_030362
					
						Alias
						Os06circ06484
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr6: 7575240-7580756  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						SMALT, Segemehl
					
						Parent gene
						
							Os06g0245800						
					
						Parent gene annotation
						
							Similar to Alanyl-tRNA synthetase. (Os06t0245800-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os06g0245800_circ_g.2 Os06g0245800_circ_g.3 Os06g0245800_circ_g.4 						
					
						Support reads
						2
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os06t0245800-01:12

General Information
CircRNA Name	Os06g0245800_circ_g.1
ID in PlantcircBase	osa_circ_030362
Alias	Os06circ06484
Organism	Oryza sativa
Position	chr6: 7575240-7580756 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	SMALT, Segemehl
Parent gene	Os06g0245800
Parent gene annotation	Similar to Alanyl-tRNA synthetase. (Os06t0245800-01)
Parent gene strand	+
Alternative splicing	Os06g0245800_circ_g.2 Os06g0245800_circ_g.3 Os06g0245800_circ_g.4
Support reads	2
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os06t0245800-01:12

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TAAGAACAACCAGAAACGAAGTATCATCTCTACGCAGCAAAATCGCGGTGCTCAAAGCAGCATC
CCTCGCAAATAAAGCTACAACTATCGATAACACGAGggtcgttgggctgtgcgggagattgcgt
cgtgaggagtatgggttcgtctcgtgagcgcggggtgctcgtgaagaccagcagctcatcaggt
ttagtaat

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	TAAGAACAACCAGAAACGAAGTATCATCTCTACGCAGCAAAATCGCGGTGCTCAAAGCAGCATC CCTCGCAAATAAAGCTACAACTATCGATAACACGAGggtcgttgggctgtgcgggagattgcgt cgtgaggagtatgggttcgtctcgtgagcgcggggtgctcgtgaagaccagcagctcatcaggt ttagtaat
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.151721325

Conservation Information
Conserved circRNAs	NA
PMCS	0.151721325

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							7578815-7575291(+)
7575328-7580217(-)
7575751-7580688(-)
						
						Potential amino acid sequence
						
							MRLRLSRVSPILNFWDRTPFYAESGGQVGDNGFLYVYGEEDAKQKAVIEINDVQKSLGNIFVHK
GTIKQGSVEVGKEIDAAVDAKLRQGAKAHHTATHLLQSALKSIIGSETSQAGSLVAFDRLRFDF
NFHRPLSEEELMKIESLVNQWVSSATHLETKVMDLQDAKNAGAIAMFGEKYGEQVRVVEVPGVS
MELCGGTHVSNTAEIRGFKIISEQGIASGVRRIEAVAGDAFVEYVCARDNYMRCLCSSLKVKAE
DVNGRVETILEELRTTRNEVSSLRSKIAVLKAASLANKATTIDNTRVVGLCGRLRREEYGFVS*
(+)
MSCWSSRAPRAHETNPYSSRRNLPHSPTTLVLSIVVALFARDAALSTAILLRRDDTSFLVVLSS
SRIVSTLPFTSSALTLRDEHKHRI*(-)
MASPLHSPELAAPTSWVADSTEADELLVFTSTPRSRDEPILLTTQSPAQPNDPRVIDSCSFICE
GCCFEHRDFAA*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	7578815-7575291(+) 7575328-7580217(-) 7575751-7580688(-)
Potential amino acid sequence	MRLRLSRVSPILNFWDRTPFYAESGGQVGDNGFLYVYGEEDAKQKAVIEINDVQKSLGNIFVHK GTIKQGSVEVGKEIDAAVDAKLRQGAKAHHTATHLLQSALKSIIGSETSQAGSLVAFDRLRFDF NFHRPLSEEELMKIESLVNQWVSSATHLETKVMDLQDAKNAGAIAMFGEKYGEQVRVVEVPGVS MELCGGTHVSNTAEIRGFKIISEQGIASGVRRIEAVAGDAFVEYVCARDNYMRCLCSSLKVKAE DVNGRVETILEELRTTRNEVSSLRSKIAVLKAASLANKATTIDNTRVVGLCGRLRREEYGFVS* (+) MSCWSSRAPRAHETNPYSSRRNLPHSPTTLVLSIVVALFARDAALSTAILLRRDDTSFLVVLSS SRIVSTLPFTSSALTLRDEHKHRI(-) MASPLHSPELAAPTSWVADSTEADELLVFTSTPRSRDEPILLTTQSPAQPNDPRVIDSCSFICE GCCFEHRDFAA(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015

circRNA

Detail information of Os06g0245800_circ_g.1

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